A Selected Chronological Bibliography of Biology and Medicine

 

Part 2A

 

c. 1810— 1857

 

 

Compiled by James Southworth Steen, Ph.D.

Delta State University

 

Dedicated to my loving family

 

This document celebrates those secondary authors and laboratory technicians without whom most of this great labor of discovery would have proved impossible.

 

Please forward any editorial comments to: James S. Steen, Ph.D., Professor Emeritus, jsteen08@bellsouth.net










 

c. 1810

Mary Anning (GB) was celebrated as the outstanding fossil collector of her time. Among the many fossils collected and prepared by her are the first ichthyosaur skeleton and the first plesiosaur skeleton known to the English community. The ichthyosaur fossil was probably discovered sometime between 1809 and 1811, when Mary was only 10 to 12 years old. And while Mary did find the majority of the remains, her brother had discovered part of the animal twelve months earlier. Most of Mary's finds ended up in museums and personal collections without credit being given to her as the discoverer of the fossils (1867). Note: Ichthyosaurs flourished c. 200M-190M B.C.E.. Pleisosaurs flourished c. 203M-66M B.C.E.

 

1810

William Hyde Wollaston (GB) isolated cystic oxide (cystine) from unusual kidney stones (1206; 2167). Note: This was the second amino acid to be discovered.

Jöns Jakob von Berzelius (SE) named it cystine (Gk. cystine, bladder) (1959).

Karl Axel Hampus Mörner (SE) was the first to isolate cystine from a protein hydrolysate (animal horn) (1268).

Richard August Carl Emil Erlenmeyer, Jr. (DE) was the first to synthesize cystine (630; 631).

 

Louis Antoine Planche (FR) observed that extracts of plant roots would turn alcoholic solutions of guaiac resin a blue color. The agent responsible for this change was found to be water-soluble and thermolabile (1477; 1478). This represents an early account of enzymatic activity.

 

Joseph Louis Gay-Lussac (FR) found that grape juice preserved for over a year by the Appert method would upon opening ferment within a few days. An unopened control bottle of Appert’s grape juice remained unchanged.

Finding that bottles of Appert’s preserves showed no oxygen Gay-Lussac concluded that this gas must play a vital part in the fermentation process. He introduced some small and intact grapes into a bell jar standing over mercury. He filled the jar several times with hydrogen gas to displace any oxygen and he then ruptured the grapes by means of an iron rod and watched the effect. For twenty-five days no fermentation had taken place, but it soon occurred when he admitted into the bell jar some bubbles of oxygen. The oxygen introduced was soon proved to have disappeared while carbon dioxide was evolved. From this Gay-Lussac concluded that oxygen was necessary to start fermentation but not for its continuance. Grape juice, which had been preserved and poured into a fresh bottle, could be re-preserved by subsequent heating. These results obtained with grape juice were also found to apply to preserved meat, fish, and mushrooms (737).

 

Pierre André Latreille (FR) originated the invaluable notion of type species of a genus. This concept, quite new at the time, is particularly known from the Table des Genres avec l'Indication de l'Espèce qui leur sert de Type (1074). Note: Similarly, he favored the method of naming families after one of the constituent genera, rather than some defining feature of the group, implicitly designating a type genus for the family.

 

Robert Brown (GB) was the first to demonstrate that the gymnosperms (conifers, ginkgo, and cycads) are a group apart from the angiosperms (flowering plants) and distinguished from these in having naked ovules. He was the first to explain the floral morphology and pollination in the Asclepiadaceae (Milkweed family) (299).

Phillip Parker King (GB) quotes Brown, “It would entirely remove the doubts that may exist respecting the point of impregnation, if cases could be produced where the ovarium was either altogether wanting, or so imperfectly formed, that the ovulum itself became directly exposed to the action of the pollen…such, I believe, is the real explanation of the structure of Cycadeae, of Coniferae, of Ephedra, and even of Gnetum.” (1006)

 

Gaspard Laurent Bayle (FR) pointed out that tubercles might be present in patients before symptoms appear and correlated tubercles with cavity formation. He described acute miliary tuberculosis, tubercular laryngitis, lymphadenitis and enteritis, and insisted that tuberculosis was a specific disease, not a condition brought on by another disease (89).

 

Franz Joseph Gall (DE-FR) and Johann Gaspar Spurzheim (DE) were the first to point out that the nerve tissue we call gray matter is important in higher thought processes while the white matter represents connecting nerves. They demonstrated that the cranial nerves issue from the medulla oblongata and not the cerebral hemisphere. They also promoted the belief that character traits and mental aberrations are organic, inborn, god given; espousing the idea that a careful study of the external appearance of the skull could be used to predict the talents and mental characteristics of the possessor (phrenology) (726; 727).

 

Thomas Copeland (GB) wrote the first English book on general colo-rectal surgery (430).

 

1811

“ On laying bare the roots of the spinal nerves, I found that I could cut across the posterior fasciculus of nerves which took its origin from the posterior portion of the spinal marrow without convulsing the muscles of the back, but that, on touching the anterior fasciculus with the point of the knife, the muscles of the back were immediately convulsed.” Charles Bell (96)

 

Georges Léopold Chrétien Frédéric Dagobert Cuvier (FR) and Alexandre-Théodore Brongniart, Jr. (FR) concluded from their studies of sedimentary rock strata in the Paris Basin that the relative position of a layer is an indication of its relative age (295; 464; 465).

 

Lorenzo Romano Amedeo Carlo Avogadro (IT) proposed that two equal volumes of gases of any type, if kept at the same pressure and temperature, contain equal numbers of molecules. This became known as Avogadro’s law (59).

 

Joseph Louis Gay-Lussac (FR) and Louis Jacques Thénard (FR) determined the elementary composition of sugar for the first time (736).

 

Henri Braconnot (FR) isolated d-mannite, the sweet principle of manna, from Agaricus mushrooms. He claimed that it was non-fermentable (231; 232). The manna that is used as an agreeable food in the East, and as a purgative for children in the West is caused to flow from the Tamarix mannifera shrub, by the punctures of a small insect, Coccus maniparus.

 

Henri Braconnot (FR), working with mushrooms, discovered fungine (chitin), the earliest known polysaccharide (230).

Antoine Odier (FR) in his survey of the insect cuticle renamed fungine as chitine (meaning tunic in Greek) (1369). It is spelled chitin in German and English.

Charles Marie Benjamin Rouget (FR) isolated chitosan (1629).

 

Louis Odier (CH) discovered greatly enlarged and very painful nerves, which he named neuromes (neuroma) (1370).

William Wood (GB) observed and described neuromas in 24 amputation stumps (2168).

 

Caspar Wistar (US) wrote the first systematic treatise on anatomy to be published in North America (2155). His friend Thomas Nuttall (GB) named the wisteria vine for him.

 

Peter Cullen (GB) defined a case of splenitis acutus with unexplainable milky blood (454).

Alfred-Armand-Louis-Marie Velpeau (FR) described a 63-year-old florist who developed an illness characterized by fever, weakness, urinary stones, and substantial enlargement of the liver and spleen. Velpeau noted the blood of this patient had a consistency "like gruel” and speculated the appearance of the blood was due to white corpuscles (1913).

Alfred Francois Donné (FR) detected a maturation arrest of the white blood cells (541).

John Hughes Bennett (GB) used the term leucocythemia to describe this pathological condition (104).

John Hughes Bennett (GB) and Rudolph Ludwig Karl Virchow (DE) were the first to describe chronic myeloid leukemia; Virchow’s description was post-mortem (104; 1925).

Rudolf Ludwig Carl Virchow (DE) coined the term leukemia and was the first to describe the abnormal excess of white blood cells in patients with the clinical syndrome described by Velpeau and Bennett (1934). Acute lymphoblastic leukemia (ALL) or acute lymphoid leukemia is an acute form of leukemia, or cancer of the white blood cells, characterized by the overproduction of cancerous, immature white blood cells—known as lymphoblasts.

 

Elisha North (US) wrote an essay concerning the epidemic of spotted fever in New England. This is the first book on cerebrospinal meningitis; in it North recommended the use of the clinical thermometer, not in general use until the time of Wunderlich (1354).

 

Gaspard Vieusseux (CH), in 1810, was the first to describe lateral medullary infarction, noting, "Vertigo, unilateral facial numbness, loss of pain and temperature appreciation in the opposite limbs, dysphasia [sic] and hoarseness, minor tongue involvement, hiccups (cured by taking up the habit of a morning cigarette) and a drooped eyelid." (1205)

Adolf Wallenberg (DE) provided a very detailed description of the clinical signs of lateral medullary infarction with accurate localization of the lesion in the lateral medulla supplied by the posterior inferior cerebellar artery (PICA). He later proved this at postmortem (2075-2077). This condition is often called Wallenberg’s syndrome.

 

John Syng Dorsey (US) successfully ligated the external iliac artery (545).

 

1812

Karl Friedrich Gauss (DE) wrote Theoria Combinationis Observationum Erroribus Minimis Obnoxia [Theory of Least Squares] (735). This is basic to the statistical evaluation of data.

 

Joseph von Fraunhofer (DE), in 1812, invented an achromatic objective consisting of two different lenses in contact with one another. He and Pierre Louis Guinand (CH) developed ways to free optical glass of imperfections. They continued to improve lenses and prisms with additional inventions (926).

 

Joseph von Fraunhofer (DE) invented the spectroscope and discovered the 574 dark lines in the solar spectrum. These lines were later named for him. He also measured the wavelength of sodium light by means of diffraction grating (1973).

 

Konstantin Sigizmundovich Kirchhoff; Gottlieb Sigismund Constantin Kirchhoff (DE-RU) obtained the hydrolysis of starch to sugars in dilute acids (sulfuric, nitric, oxalic, etc.) (1011).

 

Johann Jacob Paul Moldenhawer (DE) had the idea that vascular bundles are complex structures composed of xylem and phloem (1260).

 

Louis Nicolas Vauquelin (FR) discovered phospholipids while studying material extracted from brain tissue. He also noted that the medulla oblongata and the spinal cord contain more fatty material and less protein than grey matter (1911).

 

William Charles Wells (US-GB), in an often-ignored study, applied the principle of natural selection to the evolution of man. The application was limited to the question of how different skin colors arose (2115).

Patrick Matthew (GB) predates Charles Robert Darwin (GB) in the proposal of a theory of natural selection. In his book, On Naval Timber and Arboriculture, he wrote, "As nature, in all her modifications of life, has a power of increase far beyond what is needed to supply the place of what falls by Time's decay, those individuals who possess not the requisite strength, swiftness, hardihood, or cunning, fall prematurely without reproducing -- either a prey to their natural devourers, or sinking under disease, generally induced by want of nourishment, their place being occupied by the more perfect of their own kind, who are pressing on the means of subsistence. There is more beauty and unity of design in this continual balancing of life to circumstance, and greater conformity to those dispositions of nature which are manifest to us, than in total destruction and new creation. It is improbable that much of this diversification is owing to commixture of species nearly allied, all change by this appears very limited, and confined within the bounds of what is called species; the progeny of the same parents, under great differences of circumstance, might, in several generations, even become distinct species, incapable of co-reproduction." (1219)

 

Napoleon's army was attacked again by typhus and dysentery (the "bloody flux") during his invasion of Russia, both on the march eastward and again on the return, where disease was exacerbated by severe cold and starvation. It is estimated that only about 30,000 survived of the nearly 600,000 troops that began the campaign (1030).

 

René-Joachim-Henri Dutrochet (FR) was one of the first to place the rotifers in their own separate natural group, the class Rotifera (594). They are in the phylum Aschelminthes (or Nemathelminthes).

 

Kaspar Friedrich Wolff; Caspar Frederick Wolff) (DE-RU) described the embryonic development of the intestines in the chick (2163-2165).

 

Guillaume Dupuytren (FR) was the first to successfully excise the lower jaw, in 1812 (586).

 

Antonio Scarpa (IT) pinned an authoritative work on hernia, from which are derived the eponyms Scarpa's fascia and Scarpa's triangle of the thigh (1673).

 

John Collins Warren (US) gave probably the first description of the relationship between cardiac death and ‘ossification’ of the coronary arteries. He further drew attention to poor realization in the medical community of the correlation between the extent of such ossification and the pre-mortem symptoms of angina, to the extent that it is clear that some of his patients had what was later recognised to be silent ischaemia (2092).

 

Georges Léopold Chrétien Frédéric Dagobert Cuvier (FR) was the first to extend the system of classification to fossils. He suggested that fossils found in the area around Paris are thousands of centuries old. This casual observation pushed the age of the earth well beyond its commonly accepted limits. Cuvier also published a paper explaining that the fossil animals he studied bore no resemblance to anything still living. In short, Cuvier proposed the theory of extinction. He coined the word pterodactyl (wing-finger). For his discoveries related to fossils he is considered the founder of paleontology (457-459; 461; 463; 1639). Through the rigorous application of his correlation theory Cuvier was able to correctly identify entire animals from a few bones and demonstrate that these animals were indeed extinct, e.g. he identified pterosaurs as flying reptiles. His conclusions represented the foundation of modern paleontology, yet they would be largely ignored for many years (460).

 

The New England Journal of Medicine was founded (1144).

 

Proceedings of the Royal Academy of Sciences of Amsterdam was founded.

 

1813

"But these are deeds which should not pass away,

And names that must not wither." George Gordon (Lord Byron) (775)

 

David Brewster (GB) suggested that viewing would be improved if the front element of a microscope’s objective lens could be immersed in the liquid in which the object of study was mounted. It is he who recommended that oil immersion would improve achromatic viewing (248).

 

Jöns Jakob Berzelius (SE) established that the elements in inorganic substances are bound together in definite proportions by weight (the law of constant proportions) (141; 142).

 

Bernard Courtois (FR) was the first to prepare iodine when he observed purple vapors rising from kelp ashes that he had acidified with sulfuric acid and heated. The purple vapors condensed on a cold surface, forming nearly black crystals (445). Note: He performed this experiment in 1811.

Joseph Louis Gay-Lussac (FR) reported that the substance he calls iode is likely an element (738). Later he is confident it is a new element (739).

Humphrey Davy (GB) tested the substance Courtois had discovered and pronounced it a new element, which he named iodine (488).

 

Michel-Eugène Chevreul (FR) was the first to report the participation of water during the saponification process (367).

 

Michel-Eugène Chevreul (FR) was the first to isolate the following acids: margaric (a mixture of palmitic and stearic acids), butyric, caproic, capric, and isovaleric (acide phocénique), along with stearic (stearine), palmitic, and oleic, the three most common and important constituents of fats and oils. He determined that spermaceti, the wax-like substance from the head of the sperm whale, when boiled with alkali, produced soap, later identified as potassium palmitate, but did not yield glycerol as a residue. The residue was insoluble in water but soluble in alcohol and ether. He called it cetin, which was later identified as cetyl alcohol. He was the first lipid specialist to discover the concept of fatty acids and clearly demonstrate that fats have the structure of ethereal salts and are a combination of glycerol and fatty acids, easily separated by saponification (366; 370).

 

Jean Vincent Félix Lamouroux (FR) provided an important advance in the study of the algae when he became the first to propose a system of classification into major taxa based in part on color. He proposed a general classification for the marine algae, which he divided into Fucaceae, Florideae, Dictyoteae, Ulvaceae, Alcyonideae, and Spongodieae. Except for the last two, these groups have been maintained in present classifications. He discovered two distinct types of reproduction among the Florideae 1) tubercles called seeds (cystocarps) and 2) capsules called tetrasporocysts. Lamouroux described many new genera of algae (1064; 1065).

 

Frederick Pursh; Frederick Traugott Pursh (DE) described plants from some forty European collections along with some brought back from "The Lewis and Clark Expedition", or "Corps of Discovery Expedition" (1804–1806) in his Flora Americae Septentrionalis (1527). He is honored by the genus Purshia (Rosaceae).

 

Augustin Pyramus de Candolle (CH), in his book Théorie Élémentaire de la Botanique, introduced the word taxonomy to mean the classification of plants based on their gross anatomy (498). He was the first to perceive the major trends of floral evolution in the angiosperms. In 1824, he initiated the monumental Prodromus Systematis Naturalis Regni Vegetabilis, which proposed to classify and describe every species of known seed plant including its ecology, phytogeography, and evolution. De Candolle produced the first seven volumes; the remaining 10 were completed under editorship of his son. His system of plant classification is largely in use today (499; 500).

 

John Hay (GB) gave an excellent account of a family exhibiting hereditary hemophilia (853). See, Al-Zahrawi, c.1000

 

Thomas Bateman (GB) described papular urticaria, calling it lichen urticus. He said that its first appearance was in the form of irregular inflamed wheals, so closely resembling the spots excited by the bites of insects as almost to deceive the observer. The inflammation subsides in a day or two leaving small, elevated, itching papules. The old wheals subside while new ones appear in succession until the whole body and limbs are spotted with papules which here and there become confluent in small patches. Both the wheals and the papules are accompanied by intense itching (84). Note: This condition, as of the year 2016, is considered to be a hypersensitive reaction to insect bites.

 

Francois Magendie (FR) and Gilbert Breschet (FR) observed that rabies could be induced in healthy dogs using the saliva from rabid humans and that it could be transmitted from carnivores to herbivores. Although they did this work together around 1813-1820 Magendie published in 1821 and Breschet waited until 1840 (63; 245; 1179).

 

Francois Magendie (FR) proved that the stomach is passive rather than active in vomiting. This was essentially correct; however, he did fail to observe the active role of the pyloric end of the stomach (1187).

 

Francois Magendie (FR) showed that the epiglottis is not necessary for swallowing, which disproved the accepted doctrine that the epiglottis was necessary to cover the glottis to prevent food from entering the trachea (1176).

 

Antonio Scarpa (IT) presented the first illustrations of arteriosclerosis (1671).

 

Felix Vicq d'Azyr (FR) discovered the claustrum, a thin layer of grey matter outside the external capsule of the brain, dividing it from the white matter of the insula (1918).

 

Henry M. Onderdonk (US), in 1813, successfully ligated the femoral artery (1376).

 

1814

“ Tout m ́dicament d’ailleurs n’est pas ́galement bien indiqu ́ ` toute heure” (“All medicines are not equally indicated effective given at different hours of the day”) Julien-Joseph Virey (1940).

 

 Jean-Jacques Colin (FR), Henri-Francois Gaulthier de Claubry (FR), and Friedrich Strohmeyer (DE) independently discovered that iodine reacts with starch to form a blue color (405; 1816).

Francois-Vincent Raspail (FR) introduced the starch-iodine reaction into botanical microtechnique. He described the distribution of starch in flower, fruit, and embryo of the Gramineae. He also introduced the frozen section technique (71; 1547).

 

William Forsyth (GB), in 1802, first described the use of lime-sulfur against powdery mildew on fruit trees (701).

David Weighton (GB), in 1814, suggested a mixture of sulfur with lime water to treat mildew on fruit trees (2107; 2108).

 

Benjamin Collins Brodie (GB) reported, "Respecting the functions of the stomach, I divided these nerves [the vagi] in the neck of a dog, for the purpose of ascertaining the influence which they possess on the secretion of the gastric juice…. We may conclude that the suppression of the secretions…sufficiently demonstrate, that the secretions of the stomach and intestines are very much under the control of the nervous system." (289)

 

Karl Frederich Burdach (DE) observed that, "…in the frog, the little ovum freed from the ovary is carried to the far distant orifice of the oviduct; in birds, however, and in mammals, as in man, the tube is connected to the ovary and, having encircled that, takes that fluid into itself; this movement next is accomplished through the swelling of the tube and the filling of its vessels." He further noted, "The fetus just produced freely swims in the amniotic liquid" and "…the amnion, in fact, rolled around the umbilicus and investing the fetus, is seen to form the skin." (328; 1244) Amnion comes from the Greek amnos, meaning lamb; named no doubt, in some ancient sheepfold when the ewes were giving birth.

 

Julien-Joseph Virey (FR) envisioned biological rhythms to be innate in origin and controlled by living clocks entrained by periodic environmental changes, such as the day-night alternation in light and darkness. He also reported that the effects of drugs vary according to their administration time. But, above all, he collected and published quantified time series that demonstrated human circadian and annual mortality rhythms. Statistical analysis of Virey's data using modern time series methods confirms his deduction that human mortality exhibits rhythmicity (1587; 1940).

 

Robert C. Graham (GB) first described a clinical condition later to be called Leriche syndrome (781).

René Leriche (FR) described a constellation of symptoms in male patients, which became known as Leriche syndrome or aortoiliac occlusive disease. The syndrome consists of the following triad: (a) absent or diminished femoral pulses; (b) intermittent claudication with pallor, coldness and diffuse muscle atrophy of both the lower extremities; and (c) impotence. Leriche believed that segmental atherosclerosis caused this syndrome and proposed that restoration of the blood supply could be curative. He suggested in the 1920s that resection of the obliterated segment and repair with a vascular graft would be the ideal treatment for this syndrome (1102; 1103).

Jean Kunlin (FR) realized Leriche’s prediction when in 1947 he successfully performed the first end-to-side anastomosis using an autogenous venous graft (1052; 1053). Note: because of fibrotic conditions end-to-end anastomoses could not be done on the patient in the 1951 paper. Kunlin had no other choice but end-to-side implantations of the venous graft into the femoral artery above and below the resected area. Thus, the bypass graft procedure was born by serendipity.

Jacques Oudot (FR) was the first to resect the terminal aorta for the Leriche syndrome. He replaced the aorta with a preserved 24-day-old homologous aortic graft using end-to-end anastomoses. Six months later, because of thrombosis of the right iliac limb of the graft, Oudot placed a crossover graft from the left distal external iliac to the right external iliac—the first extra-anatomic bypass graft (1382).

 

Abraham Colles (IE) wrote a paper on treatment of fracture of the carpal extremity of the radius, which was so masterful that this fracture came to be known as a Colles fracture. He treated the fracture with tin splints to stabilize the wrist after closed reduction of the fracture (406).

 

c. 1815

Friedrich Benjamin Osiander (DE) invented uterine traction forceps of superior design (544).

 

1815

"Why has not anyone seen that fossils alone gave birth to a theory about the formation of the earth, that without them, no one would have ever dreamed that there were successive epochs in the formation of the globe?" Léopold Chrétien Frédéric Dagobert Cuvier (462). See: Anaximander, c. 580 B.C.E. and Xenophanes c. 570-480 B.C.E.

 

William Prout (GB) speculated that the atomic weights of all the elements are exact multiples of that of hydrogen or half that of hydrogen (1511).

 

Jean Baptiste Biot (FR) showed that when organic compounds are liquid or in solution they might, in effect, rotate polarized light either clockwise or counterclockwise. He suggested that this was due to an asymmetry that might exist in the molecules themselves (163). This represents the origin of stereochemistry.

Jean Baptiste Biot (FR) and Jean-Francois Persoz (FR) gave the name dextrin to the sugar solution produced when starch is hydrolyzed with mineral acids because the resulting solution rotates polarized light to the right (164).

 

Heinrich August Vogel (DE) discovered that glucose reduces heavy metals dissolved in alkaline solution causing deposition of the metal and oxidation of the glucose. This phenomenon would later be used as the basis for several tests for sugars (1941).

Carl A. Trommer (DE) introduced alkaline copper sulfate solution as a sensitive test for glucose (1881).

Hermann Christian Fehling (DE) greatly improved on the sensitivity of Trommer’s test for glucose with his aqueous solution of copper sulfate, sodium tartrate, and sodium hydroxide (646).

Friedrich August Kekulé von Stradonitz (DE) interpreted this reaction to indicate that glucose is an aldehyde. Ref

Rudolf Fittig (DE) stated that the simple sugars contain aldehyde groups (681).

Heinrich Kiliani (DE) proved this to be correct (1003).

 

Konstantin Sigizmundovich Kirchhoff; Gottlieb Sigismund Constantin Kirchhoff (DE-RU) preformed an experiment, which converted four parts of water, two parts of starch, and malt into a starch paste. This paste began to liquefy into sweet syrup. His results showed that gluten had the capacity to convert a larger quantity of starch into sugar. Thus, Kirchhoff laid the foundation for the discovery of amylase (1010).

 

William Kirby (GB), William Spence (GB) and Agostino Bassi (IT) were among the first to suspect that fungi could infect insects (80; 81; 1009). Note: William Kirby (GB) and William Spence (GB) wrote An Introduction to Entomology (first edition in 1815). This was the first modern entomology text (1009).

 

Michel-Eugène Chevreul (FR) demonstrated that the sugar from the urine of a diabetic is identical with grape sugar (glucose). This was an important step in recognizing that diabetes is a disease of sugar metabolism (368).

 

Abraham Colles (IE) was the first to tie the subclavian artery (407).

Charles Aston Key (GB) successfully ligated the subclavian artery for aneurysm at the axilla (1001).

 

Johann Friedrich Meckel (called the Younger) (DE) produced a teratology, which was the first comprehensive, analytical description of human congenital birth defects. He was one of the first to recognize that certain defects represent merely the persistence of anatomical conditions that are normal at an earlier stage of the embryo, e.g. cleft palate and ectopia cordis (abnormally superficial position of the heart). Other defects he attributed to local disturbances of growth during embryonic development (1229; 1231).

 

Francisco Romero (ES), in the opinion of some, became the first heart surgeon when, in 1801, he performed an open pericardiostomy to treat a pericardial effusion. The patient was a 35-year-old farmer named Antonio de Mira from whom five pounds of bloody fluid was drained after which he made a good recovery; going back to work in 4 months. Three years after the operation his only complaint was pain in the incision. Romero presented his work at the Society of the School of Medicine in Paris in 1815 (45; 1784).

Henry C. Dalton (US) sutured a pericardial wound. The operation took place on September 6, 1891 (469).

Daniel Hale Williams (US), on 9 July, 1893, treated a stab wound victim by sewing up a tear in the pericardium but leaving the heart muscle itself alone, allowing a small nick there — about one tenth of an inch in length — to heal on its own (2136). This does not qualify as open-heart surgery.

 

Benjamin Winslow Dudley (US), a remarkable surgeon in "rural" Kentucky, performed 225 lithotomies, the first 100 without death, successfully trephinated the skull in five patients, and successfully ligated the subclavian artery for axillary aneurysm and the common carotid for an intracranial aneurysm.

While Dudley was professor of anatomy and surgery in the medical department of Transylvania University in Lexington, it was considered equal to the best medical schools in the east. As a youth he managed to study with a local practitioner on the Kentucky frontier then graduate with an M.D. degree from the University of Pennsylvania, in 1806. After a brief period of practice in Kentucky he raised the funds to send himself to Europe where he studied with many of the medical luminaries of his time before returning to Lexington, Kentucky (569; 1198).

 

Guillaume Dupuytren (FR) successfully ligated the external iliac, 1815 (587).

 

Jacques Lisfranc (FR) devised an operation for partial amputation of the foot at the tarsometatarsal articulation (1125).

 

Jean Etienne Dominique Esquirol (FR) distinguished between “petit mal” and “grand mal” (632).

 

1816

John Vaughan Thompson (IE), in 1816, trailed a fine muslin hoop net in the seas off Madagascar to collect small life forms. See, Thompson, 1829.

John Cranch (GB) almost simultaneously used fine nets to collect small aquatic life forms during the James Kingston Tackey expedition to the river Zaire (Congo) (1086). Note: Thompson and Cranch were possibly the first to collect and describe plankton.

 

Jean Vincent Félix Lamouroux (FR) described marine hydrozoa and bryozoa (1065).

 

William Jackson Hooker (GB) wrote British Jungermanniae, which established hepaticology (the study of liverworts) as an independent discipline (913). He became director of Royal Botanic Gardens, Kew, in 1841. An energetic director, he and his son, Joseph Dalton Hooker, oversaw a rapid growth in the garden's library and land holdings taking it to a world-famous status. Joseph Dalton Hooker became director in 1865.

 

William Prout (GB) showed that the urine of a boa constrictor contains 90 percent uric acid (1512).

William Prout (GB) discovered that uric acid reacts with ammonia to yield murexide, which has a violet color. This color reaction became the basis for a delicate test for uric acid (1513).

 

René-Joachim-Henri Dutrochet (FR) demonstrated the analogy between the fetal envelopes in ovipara and vivipara suggesting a unity of the main features in the development of animals (595).

 

Karl Ferdinand von Graefe (PL-DE), in 1816, and Philbert Roux (FR), in 1819, independently performed the first closure of congenital cleft soft palate (1799; 1980).

 

Marie-Jules-César Lelorgne de Savigny (FR) established the homology of the jaws with other appendages of all insects whether biting or sucking (507).

Martin Heinrich Rathke (DE) in a paper on the isopod, Asellus, then on many other invertebrates, recognized that antennae, jaws, and feet exhibit developmental homology (1561; 1563).

 

John King (US), in 1816, operated for abdominal pregnancy, saving both mother and child (1005).

 

1817-1823

The first great cholera pandemic of the 19th century swept Asia, probably originating near Calcutta and spreading from there throughout Southeast Asia, Japan and China. The death toll from this outbreak is not known, however, based on the 10,000-recorded deaths among British troops, researchers estimate that hundreds of thousands across India succumbed to the disease. In 1820, 100,000 people died on the Indonesian island of Java alone. Although it spread as far as Southern Russia and the Middle East, an exceptionally cold winter in 1823-24 kept it from reaching Western Europe (1030). Note: Scholars usually refer to a wave of seven cholera pandemics, and generally describe them as occurring 1817-23, 1826-37, 1846-63, 1865-75, 1881-96, and 1902-23, and 1961-present (1238).

 

1817

"Nature has neither core nor shell; she is everything at once." Johann Wolfgang von Goethe (1977)

 

Pierre-Jean Robiquet (FR) was the first to note the chemical nature of narcotine and isolate it in a pure state (1618).

 

Johann Baptist von Spix (DE) and Carl Friedrich Philipp von Martius (DE) conducted zoological and botanical explorations of Brazil (1817–1820) (1783).

 

Francois Magendie (FR) isolated emetine (1178).

Edward Bright Vedder (US) was the first to demonstrate the value of emetine in the treatment of amoebic dysentery (1912).

Leonard Rogers (GB) established the clinical use of emetine in the treatment of amoebic dysentery (1621; 1622).

 

Pierre-Joseph Pelletier (FR) and Joseph-Bienaimé Caventou (FR) isolated and named chlorophyll (chlorophyll, Greek, green leaf) (1441).

 

Amos Eaton (US) wrote his Manual of Botany for the Northern States, which was an important predecessor of Gray's Manual (604).

 

Leopold Gmelin (DE) described the bile acids and developed the Gmelin Test for detecting the presence of bile pigments (1857). He was also the first to apply the names ester and ketone to two classes of organic compounds. His most notable contribution, however, was the Handbuch der Chemie, first published in a 2-volume version in 1817 and 1819 and later enlarged to 13 volumes. The work was translated into English as Handbook of Chemistry (19 vol., 1848-71) (751).

 

Thomas Bateman (GB) and Robert Willan (GB) first described and later assigned a name to molluscum contagiosum, a cutaneous and mucosal eruption of a contagious nature (85; 86).

William Henderson (GB) and Robert Paterson (GB) described the intracytoplasmic inclusion bodies now known as molluscum bodies or Henderson-Paterson bodies (304; 868; 1429).

Max Juliusberg (DE), Udo J. Wile (US), and Lyle B. Kingery (US) were able to extract filterable virus from lesions of molluscum contagiosum and show transmissibility (984; 2130).

Ernest William Goodpasture (US) later described the similarities of molluscum and vaccinia (771).

 

Henry Jacob Bigelow (US) compiled a survey of the medicinal plants of the United States. It was one of the first two books in America to include plates printed in color (155). Bigelow also co-authored the first national pharmacopoeia in 1820.

 

Alexandre John Gaspard Marcet (CH-GB) found xanthine in kidney stones—naming it xanthic oxide (1206).

 

Karl Friedridi Philipp Martius (DE) was the first to describe the hyphomycetes (1213).

 

Christian Heinrich Pander (LV), from his study of over a thousand chick eggs announced the trilaminar structure of the chick blastoderm, a terminology that he coined. He suggested that developing chick embryos contain three germ layers. The delamination of the blastoderm (young embryo) results in the formation of the mucous membrane (endoderm) and the serous membrane (ectoderm). The serous membrane undergoes delamination, giving rise to a third layer, the vascular membrane (mesoderm) (1405; 1406).

 

Francois Magendie (FR) wrote the first modern physiology textbook in which the importance of nitrogenous foods (protein) in the diet of mammals was demonstrated (1177).

 

James Parkinson (GB) wrote a little known medical monograph entitled Observations on the Nature and Cure of Gout, however, his Essay on the Shaking Palsy gained him immortality in the annals of medicine. He described what became known as Parkinson’s disease thus, “Involuntary tremulous motion, with lessened muscular power, in parts not in action and even when supported; with a propensity to bend the trunk forwards, and to pass from a walking to a running pace: the senses and intellect being uninjured” (1418; 1419).

Jean-Martin Charcot (FR) and Edme Félix Alfred Vulpian (FR), four decades later, added rigidity to Parkinson's excellent clinical description and attached the name la maladie de Parkinson [Parkinson's disease] to the syndrome (361; 362).

Edouard Brissaud (FR) and Henry Meige (FR) suggested that paralysis agitans (Parkinson’s) might be due to a vascular lesion in the substantia nigrans of the mid-brain (285).

Constantin Trétiakoff (FR) provided pathological evidence, which supported Brissaud’s suggestion (1875).

Charles Foix (FR) and Jean Nicolesco (FR) showed that the specific lesions in Parkinson’s disease are in the substantia nigra of the mid-brain (697; 698).

Herbert Ehringer (AT) and Oleh Hornykiewicz (AT) showed that brain dopamine is lower than normal in Parkinson's disease patients (620).

Walther Birkmayer (AT) and Oleh Hornykiewicz (AT) injected Parkinson’s disease patients with L-DOPA thereby producing a spectacular improvement of all motor deficits of the patients (168).

Oleh Hornykiewicz (AT) proved the existence of a nigro-striatal dopamine pathway in the human brain (922).

George Constantin Cotzias (GR-US), Paul S. Papavasiliou (US), and Rosemary Gellene (US) demonstrated the effectiveness of accommodating patients to large daily dosages of L-DOPA in the treatment of Parkinson's disease (442; 443).

Jan J. Korten (NL), Antoine Keyser (NL), Ed M.G. Joosten (NL), Fons J.M. Gabreëls (NL), and Eileen Critchley (GB) introduced the use of carbidopa (Sinemet) as a treatment for Parkinson's disease (4; 449; 1035).

Eric Olaf Backlund (SE), Per-Ola Granberg (SE), Bertil Hamberger (SE), Evert Knutsson (SE), Anders Martensson (SE), Göran C. Sedvall (SE), Ake Sieger (SE), and Lars Olson (SE) surgically transplanted parts of the adrenal medulla autologously into the brain of a patient with severe Parkinson's disease (62). The results have been promising.

Mihael H. Polymeropoulos (GR-US), Christian Lavedan (US), Elizabeth Leroy (US), Susan E. Ide (US), Anindya Dehejia (US), Amalia Dutra (US), Brian Pike (US), Holly Root (US), Jeffrey Rubenstein (US), Rebecca Boyer (US), Edward S. Stenroos (US), Settara Chandrasekharappa (IN-US), Aglaia Athanassiadou (GR), Theodore Papapetropoulos (GR), William G. Johnson (US), Alice M. Lazzarini (US), Roger C. Duvoisin (US), Giuseppe Di Iorio (IT), Lawrence I. Golbe (US), and Robert L. Nussbaum (US) discovered a mutation in the alpha-synuclein gene identified in families with Parkinson's disease (1488).

 

Thomas Bateman (GB) and Robert Willan (GB) described the nature of recurrent Herpes simplex virus infection accurately as "a restricted group of localized vesicles with a short, self-limited course." This publication was particularly important because it contained descriptions of herpes iris (now known as erythema multiforme) and eczema due to external irritation. It also contained descriptions of molluscum contagiosum (86).

 

Eugène Houssard (FR) described chronic subdural hematoma which he thought was of inflammatory origin (925). Chronic subdural hematoma had been recognized by Wepfer in 1657.

 

India experienced an outbreak of cholera.

 

The first issue of the Journal of the Academy of Natural Sciences of Philadelphia was published. It is the first natural history journal from North America.

 

1818

"All truth passes through three stages. First, it is ridiculed. Second, it is violently opposed. Third, it is accepted as being self-evident.” Arthur Schopenhauer (DE) (1699)

 

Jöns Jakob Berzelius (SE) discovered selenium (143).

 

Pierre-Joseph Pelletier (FR) and Joseph-Biènaimé Caventou (FR) reported the isolation of pure strychnine from the beans of Strychnos ignatii (Saint Ignatius’s bean) and Strychnos nux vomica (1442; 1443).

 

Michel-Eugène Chevreul (FR) identified what he called cholestérine (cholesterol) (Gk. Chole, bile + stereos, solid) as an unsaponifiable fat (369).

 

Georg August Goldfuss (DE) coined the name Protozoa (Gk, protos, first, zoon, animal) but he did not restrict it just to the protozoa as we know them today (759).

 

Giovanni Battista Amici (IT) described circulating protoplasm in Chara cells (31).

 

Pieter de Riemer (NL) appears to have been the first to freeze tissues in order to permit fine sectioning and to use anatomical sections for anatomical illustration (505).

 

Thomas Nutall (GB), while in North America, worked on the Torrey and Gray Flora of North America, and three volumes of an updated version of F. Andrew Michaux's North American Sylva. His Genera of North American Plants and a Catalogue of the Species of the Year 1817 was the first of its kind prepared by an on-the-spot American botanist (965; 1365).

 

Étienne Geoffroy Saint-Hilaire (FR) wrote Philosophie Anatomique, in which he asked the question: "Can the organization of vertebrated animals be referred to one uniform type?" The answer for Geoffroy was yes: he saw all vertebrates as modifications of a single archetype, a single form. Vestigial organs and embryonic transformations might serve no functional purpose, but they indicated the common derivation of an animal from its archetype. Geoffroy spent much time drawing up rules for deciding when structures in two different organisms were variants of the same type -- in modern terminology, when they were homologous. He observed the way embryos develop in different animals and that the central nervous system of an insect lies along its belly while that of a human lies along its back. From this he theorized that they represented two lineages from a common ancestor (1648). This work provided important evidence, which Darwin used in his On the Origin of Species by Means of Natural Selection.

 

Christian Gottfried Ehrenberg (DE) while studying Syzygites megalocarpus (Sporodina grandis) described the fungal zygospore for the first time. This was the first recognition of sex in the fungi (614).

 

John Cheyne (GB) and William Stokes (IE) described what came to be called Cheyne-Stokes respiration. This is characterized by breathing with rhythmical variations in intensity, i.e., occurring in cycles (372; 1806).

Camille Biot (FR) described an abnormal pattern of breathing characterized by groups of quick, shallow inspirations followed by regular or irregular periods of apnea. In common medical practice, Biot's respiration is often clinically equivalent to Cheyne-Stokes respiration (162). Note: This breathing pattern is seen in medullary compression of the brain, the Biot sign.

 

Valentine Mott (US) was the first to ligate the innominate artery for aneurysm. The patient was a fifty-seven-year-old sailor at New York Hospital. The anesthesia administered was a drink containing seventy drops of tincture of opium. The patient survived for twenty-five days (1271).

Elias S. Cooper (US) removed the medial end of the clavicle and a portion of the upper end of the sternum to improve the exposure to the innominate artery, this being the first time this valuable maneuver was employed during ligation of the innominate. The patient was treated for a combined aneurysm of the common carotid and subclavian arteries. He survived for nine days postoperative (429).

Andrew Woods Smyth (US) was the first surgeon to report long-term survival after ligation of the innominate artery. The operation took place at the Charity Hospital in New Orleans on 15 May 1864. Dr. Smyth ligated the right common carotid and the innominate for an aneurysm of the right subclavian artery in a 32-year-old mulatto man. The patient survived for eleven years (1768).

 

Astley Paston Cooper (GB) successfully ligated the common carotid and the external iliac arteries for aneurysms. In 1817, he performed the first recorded case of ligation of the aorta for aneurysm. The patient's right leg remained viable, but the left leg was totally ischemic, livid, and cold, and the patient died 40 hours later. Cooper’s fascia (the fascia transversalis) and Cooper’s hernia (retroperitoneal hernia) are named to commemorate him. He developed widely followed methods of treating dislocations and fractures (422-428).

 

Jean-Louis-Marc Alibert (FR) found that there is a clinical association between psoriasis and arthritis .

 

Guillaume Dupuytren (FR) was the first to successfully treat aneurysm by compression (584).

 

1819

Theodor von Grotthuss; Theodor Christian Johann Dietrich von Grotthuss (DE) was the first to recognize that light can bring about some chemical reactions as it is absorbed by molecules (1981).

 John William Draper (GB-US) rediscovered that light can bring about some chemical reactions as molecules absorb it (553). The Draper-Grotthuss law is an expression of their discovery.

 

Nicolas Clement (FR) and Charles Desormes (FR) performed experiments between 1819-1824) which led to the introduction of the term calorie for the unit of heat (24). Their calorie was a kg-calorie (modern kcal). It was defined as the quantity of heat to raise a kg of water by 1-degree C. About 1929 this definition was superseded when a committee of the British Academy of Sciences proposed the g-calorie as an alternate unit of energy (1105).

 

Henri Braconnot (FR) boiled various plant products such as sawdust, linen, and bark with acid and from the process obtained glucose (233). This had previously been obtained by the boiling of starch with acid. See, Kirchhof, 1815.

It was easy to decide that the molecule of starch was built up out of glucose units and that in many plants there must be some nonstarch material that was also built up out of glucose units. It was this nonstarch material that he was breaking down.

 

Louis Jacques Thénard (FR) is credited with the discovery of hydrogen peroxide, the enzyme catalase, and being the first to analyze an enzymatic reaction quantitatively (1829).

Oscar Loew (US) is also credited with the discovery of catalase. See, Oscar Loew, 1901.

James Batcheller Sumner (US) and Alexander L. Dounce (US) crystallized catalase (1820).

 

Joseph Louis Proust (FR) was the first to isolate leucine. It came from among the fermentation products of milk. He called it oxyde caséique (1510).

Henri Braconnot (FR) isolated and named leucine (Gk. leukos, white) from muscle tissue and wool (234).

Gerardus Johannes Mulder; Gerrit Jan Mulder (NL) was the first to secure leucine in relatively pure form and assign to it a correct formula (1288; 1291). Leucine was proved to be aminoisocaproic acid in 1891.

 

Pierre-Joseph Pelletier (FR) and Joseph-Bienaimé Caventou (FR) isolated the alkaloids brucine and veratrine (1444).

 

Simon Rudolph Brandes (DE) isolated atropine in a very pure form (236).

Philipp Lorenz Geiger (DE) and Heinrich F. Mein (DE) purified atropine from belladonna (Atropa belladonna) roots and hyoscyamine from henbane (743; 1234). Atropine is used to treat thirty plus ailments from hay fever to the tremors of Parkinson’s disease (paralysis agitans). Belladonna gets its name from the fact that ladies used it in eye drops to make their eyes sparkle. It blocks acetylcholine in the musculature of the iris resulting in dilation.

Simon Rudolph Brandes (DE) isolated an impure sample of atropine, an alkaloid, from Atropa belladonna a member of the Solanaceae. He tested its effects on himself and birds (237).

 Alfred Ladenburg (DE) isolated the alkaloid hyoscine (scopolamine) from the Solanaceae. It is similar in structure to atropine and hyoscyamine and blocks acetylcholine (1060).

 

Louis Adelbert von Chamisso; Louis Charles Adélaiede de Chamissot (DE) used the phrase alteration of generations (metagenesis) when he described the life cycle of tunicates (benthic invertebrates) (1967).

Johannes Japetus Smith Steenstrup (DK) explained how alternation of asexual and sexual generations occurs among the coelenterates, trematodes, and tunicates (1794; 1796). He is commemorated by Steenstrupia Forbes, 1846; Onchnesoma steenstrupi Koren & Danielssen, 1875; Lepidophyllum steenstrupi Odhner, 1902; Melinnacheres steenstrupi Bresciani & Lützen, 1961; Prionospio steenstrupi Malmgren, 1867; Myxicola steenstrupi Krøyer, 1856; Mimonectes steenstrupii Bovallius, 1885; Hesione steenstrupii de Quatrefages, 1866.

 

Karl Asmund Rudolphi (SE-DE) was the first to describe dicroceliasis in man. This is an infection, usually of the biliary duct, by the trematode fluke Dicrocoelium dendriticum, which Rudolphi named (1637; 1638).

Arthur Looss (DE) discovered D. hospos (1138).

Wendell H. Krull (US) and Cortland R. Mapes (US) published the full life cycle in a series of papers from 1951-1953 detailing their observations and experiments. It was known that D. dendriticum affected sheep, but everything else was a mystery. The first link in the chain was the discovery of the first intermediate host, the land snail Cochlicopa lubrica (synonym: Cionella lubrica). Next came the discovery that the slime balls coughed up by the snails could be a potential method of transfer of the parasite. Shortly thereafter, the ant, Formica fusca was found to be the second intermediate host by which sheep were infected (1042).

 

Johann Gottfried Bremser (DE-AT) provided an accurate classification of Enterobius vermicularis (Oxyuris vermicularis), distinguishing it from other oxyurids and ascarids. Commonly called the pinworm, it is the etiologic agent of the the most pervalent nematode infection of humans in temperate climates, affecting mainly children less than 12 years of age (242).

 

Charles Turner Thackrah (GB) found that blood collected after flowing over tissues, say in an open wound, coagulated more quickly than if the blood had been drawn directly from the blood vessel (1826; 1827). This was the first reported evidence for what William Henry Howell (US) would name tissue factor and Pierre Nolf (FR) would name thromboplastic substance (927; 928; 1351).

 

John Bostock (GB) gave an excellent clinical description of hay fever (summer catarrh) (201).

 

William Prout (GB) in 1816, after trying hydriodate of potash (potassium iodate) on himself in small doses and experiencing no ill effects, suggested iodine treatment for goiter. This therapy was successfully adopted by Dr. John Elliotson (1791–1868) at St. Thomas’s Hospital early in 1819 (1518).

 

René-Théophile-Hyacinthe Laënnec (FR) was the first to realize that damage in the lungs and small grey nodules scattered throughout the body are both manifestations of pulmonary tuberculosis. In 1816, he invented the stethoscope (Gk. the chest, to look at), an instrument whose use for listening to the chest was popularized by Nikolai Sergeievich Korotkoff (RU). Laënnec coined words like rales, bronchophony, pectoriloquy, and egophany. He described the symptomology, the clinical course, the physical findings, and the pathological anatomy of pneumonia, apoplexy of the lungs, gangrene, emphysema, cysts of the lung, tuberculosis, pleurisy, pleural effusion, pneumo-thorax, edema of the lungs, pulmonary tuberculosis, pulmonary abscess and gangrene, bronchiectasis, infarction, vesicular and bronchial breathing, amphoric and cracked-pot resonance, metallic tinkling, presystolic thrill which he described but misunderstood, and alcoholic cirrhosis of the liver which he described and christened. He was the first to recognize the unity of the tuberculosis condition, which had previously been thought to be several different diseases. He noted variations in the heart’s contractions with or without palpable pulse reflecting atrial fibrillation (1061-1063).

 

Karl Frederich Burdach (DE) named the globus pallelus or globus pallidus (pale clump) and the putamen (shell) and described in detail the fasciculus gracilis. He will be remembered as a neuroanatomist with his name associated as in Burdach’s fiber, fissure, nucleus, and tract. He named claustrum, brachium conjunctivum (superior cerebellar peduncle), and the cuneus. Finally, he localized vision in the posterior part of the brain and assigned the sensation of consciousness to the thalamic region of the brain (329).

William Benjamin Carpenter (GB) would take this idea further when he linked the thalamus with the cerebral cortex by saying, "The Sensory Ganglia constitute the seat of consciousness not merely for impressions on the Organ of Sense, but also for changes in the cortical substance of the cerebrum so that until the latter have reacted downwards upon the Sensorium, we have no consciousness either of the formation of ideas, or of any intellectual process of which these may be the subjects." (340)

George N. Thompson (US) and Johannes M. Nielsen (US) "concluded that the engramme system essential to crude consciousness is located where the mesencephalon, subthalamus, and hypothalamus meet." (1831)

 

Philbert-Joseph Roux (FR) performed the first staphylorrhaphy (surgical closure of a cleft palate) in 1819 (1632).

 

Guillaume Dupuytren (FR) successfully ligated the subclavian artery (585).

 

The American Journal of Science was founded.

 

1820

Eleven physicians meet in Washington, D.C., to establish the U.S. Pharmacopeia, the first compendium of standard drugs for the United States. Only 217 drugs that met the criteria of “most fully established and best understood” were admitted.

 

Pierre-Joseph Pelletier (FR) and Joseph-Bienaimé Caventou (FR) isolated and purified the alkaloid colchicine, which they extracted from meadow saffron (Colchicum autumnale), also called autumn crocus (1445).

John Want (GB) discovered that colchic (colchicine) from Colchicum autumnalis was the secret ingredient in “medicinal water” used to treat gout (2090).

Alfred Houdé (FR), in 1884, obtained a crystallized preparation of colchicine (924).

 

Friedlieb Ferdinand Runge (DE) isolated an alkaloid stimulant from mocha beans and named it caffeine (1640).

Pierre-Joseph Pelletier (FR) isolated caffeine from coffee beans (1439).

Hermann Emil Fischer (DE) and Lorenz Ach (DE) synthesized caffeine (679).

 

Henri Braconnot (FR) isolated what he called sucre de gélatine (later named glycocolle, then glycine) by heating gelatin in the presence of dilute sulfuric acid. Initially he thought glycine was a sugar because of its sweet taste (234). This was the first instance in which a pure amino acid was obtained from a protein by acid hydrolysis. Glycine is also called aminoacetic acid.

Auguste Laurent (FR) determined the correct empirical formula for the amino acid glycine (1077).

William Henry Perkin (GB) and Baldwin F. Duppa (GB) synthesized glycine by treating bromoacetic acid with ammonia. This was the first amino acid to be manufactured (1449).

Robert Brainard Corey (US) and Gustav Albrecht (US) determined the structure of the amino acid, glycine. This was the first amino acid structure solved (432).

 

Frederick Christian Accum (DE), working in England, wrote a treatise in which he described the numerous kinds of food adulteration practiced at the time and the various methods available to detect them. This treatise had an impact on most of the civilized world and spawned a generation of books on the subject in England, the United States, and Europe. Ultimately, it resulted in the modern era of food regulatory statutes (8). Thomas Wakeley (GB), Member of Parliament and founder and editor of The Lancet, set up a sanitary commission whose findings led to the passage of the Adulteration Act and Sale of Food and Drugs Act.

 

Christian Friedrich Nasse (DE) formulated Nasse's law: hemophilia occurs only in males and is passed on by unaffected females (1325).

Johann Lukas Schönlein (DE) coined the term hemophilia (1697).

 

William Norris (GB) noted that fungoid disease (melanoma), a pigmented skin cell cancer, was especially prevalent in one family under study. This of course suggested a familial influence on the appearance of the cancer (1353).

 

Charles Robert Harington (GB) and George Barger (GB) determined the structure of thyroxine and synthesized it (831).

 

Martin Heinrich Rathke (DE) reported on the embryonic development of the genital and urinary organs (1554; 1559).

 

Robert Gooch (GB) described postpartum psychosis (769).

 

1821

Stephen Elliott (US) authored the remarkable, A Sketch of the Botany of South Carolina and Georgia (625). He is commemorated by the genus Elliottia.

 

Johann Friederich Meckel (called the Younger) (DE) proposed that higher animals, during their development, pass through the stages of lower animals, through which periodical and class differences can be traced (1230).

 

Jean-Antoine Colladon (CH) observed what is now recognized as dominance when he crossed grey and white mice and found that their offspring were always either grey or white, but never intermediates. He continued these experiments as far as the third generation and it is evident that what he was doing was to make back-crosses between heterozygous grey mice and homozygous recessive white mice; but the absence of blending, itself a discovery, led him to conclude that grey mice and white mice were different species (496; 504). Some consider this the discovery of the law of hybridization.

 

Pierre-Fidèle Bretonneau (FR) proposed the general theory that different diseases are due to different specific causes. He said, “The specificity of diseases is proved by such a mass of facts, and there is probably no truth better demonstrated and more fruitful.” This concept is often referred to as the doctrine of etiological specificity. In 1821, he read a paper before the Paris Académie de Médecine in which he concluded that a sizable epidemic of throat distemper which occurred in and about Tours, France in 1819 was due to a specific disease, characterized by the formation of a false membrane in the respiratory tract. He asserted that croup, malignant angina, and scorbutic gangrene of the gums were all the same malady, a specific disease, for which he proposed the name diphtérite (diphtheritis), because of the unique membrane in the throat, clearly differentiating it from other afflictions of the throat. Diphtheritis is derived from two Greek words, one meaning skin or membrane and the other meaning inflammation. Later the designation was changed to diphtheria, the term by which the disease is known today. Bretonneau said, “it is vain to deny that contagion, if not the source of epidemics, is the source of most epidemics.” He also demonstrated the typical ulcers on Peyer’s patches in cases of typhoid fever and differentiated these from tuberculous lesions (246; 247). Pierre-Fidèle Bretonneau (FR) recorded the first successful use of tracheotomy in a case of diphtheria (1238).

 

Antonio Scarpa (IT) provides a classic description of sliding hernia, or hernia of the large bowel (1672).

 

Valentine Mott (US), in 1821, excised the right side of the lower jaw, after ligating the carotid artery (1272).

 

Charles Waterton (GB) opened the world's first nature reserve on the grounds of his estate at Walton Hall near Wakefield, England.

 

Le Journal de Physiologie Expérimentale was founded. It is the first periodical devoted exclusively to physiology (1198).

 

1822

Marie Humbert Bernard Gaspard (FR) thoroughly investigated the process of putrefaction in animals. At this time, putrefaction was believed to be intimately associated with the development of septicemia, pyemia, putrid infection, and putrid intoxication. It was supposed that these conditions were due to the absorption of putrid substances into the blood stream because the injection of putrid matter into an animal invariably led to illness or death (732; 733). Note: The modern definition of putrefaction is the enzymatic decomposition of organic matter, especially proteins, by anaerobic microorganisms, with formation of malodorous substances such as indole, skatole, cadaverine, and putrescine. Pyemia is the disease state due to the presence of pyogenic microorganisms in the blood and the formation, wherever these organisms lodge, of embolic or metastatic abscesses. Septicemia is a severe bacteremic infection usually involving invasion of the bloodstream. Sepsis is the poisoning of an individual by products of putrefaction, or a severe toxic febrile state resulting from infection with pyogenic microbes, with or without septicemia.

 

Francois Magendie (FR) introduced to medicine the effects and uses of morphine, veratrine, brucine, piperine, emetine, as well as quinine, and strychnine (1180; 1183).

 

Herbert Mayo (GB) assigned the motor nerve fibers of the face muscles to the VIIth nerve, the facial nerve, and common sensibility to the Vth nerve, the trigeminus (1224).

 

Etienne Geoffrey Saint-Hilaire (FR), Detlev Arendt (DE), Katharina Nubler-Jung (DE), Scott A. Holley (US), P. David Jackson (US), Edward Michael de Robertis (US), Yoshiki Sasai (US), Lu Bin (US), F. Michael Hoffmann (US), and Edwin L. Ferguson (US) provided evidence that there was an inversion of the dorsoventral axis during animal evolution. A conserved system of extracellular signals provides positional information for the allocation of embryonic cells to specific tissue types both in Drosophila and vertebrates; the ventral region of Drosophila is homologous to the dorsal side of the vertebrate. Developmental studies are now revealing some of the characteristics of the ancestral animal that gave rise to the arthropod and mammalian lineages, for which De Robertis and Sasai proposed the name Urbilateria (43; 44; 506; 909; 1647).

 

Pierre Salomon Ségalas d'Etchepare (FR) found that polyuria follows an injection of urea (1731).

Friedrich Wöhler (DE) was the first to introduce the idea that the role of the kidney is to keep in equilibrium the blood content of water and of those substances, which appear in the urine. He observed that the blood is alkaline while the urine is acid; the urine becomes alkaline after the ingestion of the salts of organic acids; the solutes of the urine are excreted independently of one another; and an excess of solute in the urine entails an increased excretion of water (2156). This work is the origin of the concept of osmotic diuresis.

Charles Chossat (FR) found a connection between urinary water excretion and the excretion of “animal substances,” that is, those containing nitrogen (373).

Friedrich G. Goll (CH), Karl Friedrich Wilhelm Ludwig (DE), and Max Hermann (AT) showed that urine flow could be stopped by lowering the blood pressure by either bleeding, compression of the renal artery, stimulation of the vagus, or by cutting the spinal cord and that diuresis results from a rise of blood pressure (764; 886).

Charles Robert Richet (FR) and Robert Mustard-Martin (FR) revealed that: intravenous lactose, saccharose, levulose, and dextrose each caused the immediate appearance of the sugar in the urine, followed by an increase in urine flow within the first minute (1606; 1607); the polyuria was dependent on the amount of sugar that was injected and not on its concentration (1608).

Charles Eduard Hédon (FR) and Jean Arrous (FR) discovered that the diuretic response to the intravenous injection of the same weight of various sugars is proportional to their molar concentration (859).

Emile Charles Achard (FR) and Joseph Castaigne (FR) developed a urinary test using methylene blue dye to examine kidney function. The criterion used was to find the percentage of dye, injected subcutaneously, that showed up in the urine within a 24-hour period (9).

Hermann Strauss (DE), Georges Fernand Isidore Widal (FR), André Lemierre (FR), and Adolphe Javal (FR) concluded that edema is due solely to the urinary retention of sodium chloride and introduced the low sodium diet for congestive heart failure (1813; 1814; 2123-2126).

Leo Ambard (FR) provided a way to globally evaluate renal function by using the ratio plasma urea/urine urea output. This Ambard's Constant is the initial step of the concept of clearance (30).

 

Karl Friedrich Burdach (DE), in 1822, named the cingular gyrus (the collosal convolution) and distinguished lateral and medial geniculate (knee -like bends) (329).

 

Jean Zuléma Amussat (FR) was one of the inventors of lithotripsy (33).

 

James Carson (GB) discussed the value of induced pneumothorax for the treatment of a diseased lung (342).

Carlo Forlanini (IT) proposed that ablation of the lung or artificial pneumothorax could be a positive contribution to the therapy of phthisis (tuberculosis) (700).

William Cayley (GB) used artificial pneumothorax induced by pleural incision to treat intractable hemoptysis (349).

 

Samuel Jackson (US) mentions what later became known as Korsakoff’s psychosis in a review of the peripheral neuritis of alcoholism (970).

 Sergei Sergeievich Korsakoff; Sergei Sergeyevich Korsakov (RU) emphasized the association of alcoholic polyneuropathy with a specific pattern of mental disturbance— Korsakoff’s psychosis— as follows: "This mental disorder appears at times in the form of sharply delineated irritable weakness of the mental sphere, at times in the form of confusion with characteristic mistakes in orientation for place, time and situation, and at times as an almost pure form of acute amnesia, where the recent memory is most severely involved, while the remote memory is well preserved . . . Some have suffered so widespread memory loss that they literally forget everything immediately.” This syndrome is characterized by a severe memory defect, especially for recent events, for which the patient compensates by confabulation (1033). Korsakoff wrote on paranoia (paranoia hyperphantastica as he described it in his textbook) and classified psychiatric illnesses (1034). Known as a humanitarian, he improved conditions in mental institutions. He was the first great psychiatrist in Russia. He is considered a moral genius, as were Philippe Pinel (FR) and Jean-Martin Charcot (FR).

 

Alexandre-Francois Ollivier (FR) produced experimental hospital gangrene by autoinfection (1372).

 

Storks injured by arrows (termed as pfeilstorch in German) traceable to African tribes were found in Germany in 1822 and constituted some of the earliest evidence of long distance migration in European birds (822).

 

Jean-Baptiste-Julien d' Omalius d'Halloy (BE) first used the term Terrain Cretace (Cretaceous Period) to describe chalk and greensand of Northern France (1374; 1375). The Cretaceous is usually noted for being the last portion of the Age of Dinosaurs, but that does not mean that new kinds of dinosaurs did not appear then.

 

William Daniel Conybeare (GB) and William Phillips (GB) named the Carboniferous Period in their book Outlines of Geology of England and Wales (419).

 

William Buckland (GB) published an account of how ancient hyenas lived and fed, in Kirkdale cave near Kirkbymoorside in the Vale of Pickering, North Yorkshire, England. Bones in the cave included hippopotamus (the farthest north any such remains have been found), elephant, and the remains of numerous cave hyenas and their coprolites. This is one of the first descriptions of living habits based on fossil evidence (318). Note: Material covering the bones dates them at c. 125 K BP. Note: The word archaeologists now use for fossil feces – coprolites – is a word invented by Buckland.

 

Gideon Algernon Mantell (GB), as an amateur paleontologist, discovered and described from Cretaceous England many fossilized animals including: Megalosaurus, Iguanodon, Pelorosaurus, the first discovered brachiosaur, and Hylaeosaurus (1202; 1203).

 

The German scientific society Versammlungen Deutscher Naturforscher und Ärzte was founded at Leipzig.

 

1823

Johann Wolfgang Döbereiner (DE) discovered that powdered platinum greatly accelerates certain chemical reactions (531).

Jöns Jakob von Berzelius (SE) was to later name this phenomenon katalytische kraft (catalytic power) (1961-1964). The term is coined on page 243 of the 1836b article. See, Fulhame, 1794

 

Joakim Frederik Schouw (DK) wrote Grundzüge einer Allgemeinen Pflanzengeographie in which he divided the vegetation of Europe into twenty-two botanical regions, and, thereby became a co-founder of scientific phytogeography (1700).

 

John Vaughn Thompson (IE) first discovered the nature of crustacean larvae between 1823 and 1826. By observing the transformation of a zoea into a 10-legged megalopa and the metamorphosis of swimming cyprids into benthic barnacles, Thompson not only clarified the taxonomic affinities of barnacles (originally considered baby geese, then molluscs) and zoeae (previously a distinct genus of uncertain affinity), but also paved the way for further work on invertebrate larvae (1833; 1835).

 

Thomas Stewart Traill (GB) made the first attempt to determine the fat content of human blood. He found 4.5% of liquid fat in his sample (1870). Audubon named the Traill's Flycatcher after him.

 

Herbert Mayo (GB) discovered that the optic tubercles and the crura of the brain give the pupillary reflex when the stump of the optic nerve is irritated (1225).

 

Alexandre John Gaspard Marcet (CH-GB) gave the earliest description of a case of what would later be called alcaptonuria. It was in a male infant 17 months of age. The child’s diaper was stained a deep purple color immediately after birth. The urine turned black soon after being voided and exposed to the air. It exhibited rapid blackening with alkalies and a failure of acids to restore the original color (1207).

 

Léon Rostan (FR) suggested that cerebral softening might be caused by defective arterial supply to the brain. He said, "…this change in the brain seems often to be a senile degeneration, showing great similarity to the gangrene of old age. As in the latter disease cerebral softening appears to be a disorganization in which the vessels designed to bring blood and life to the affected organs are ossified…by the process of old age." He pointed out that cerebral softening may be confined to the cortex of the brain and that this malady is accompanied by loss of memory, loss of voluntary movement, mental derangement, senile dementia, and finally by complete coma (1628).

 

Charles-Édouard Brown-Séquard (FR) proved that cerebral activity is dependent on oxygen provided by circulating blood (310).

 

Guillaume Dupuytren (FR) was the first to treat wry neck by subcutaneous section of the sternomastoid muscle, 1822 (58). See, Valentine Mott, 1818.

 

George James Guthrie (GB) wrote the first systematic English textbook on ophthalmic surgery (819).

 

William Buckland (GB) found a human skeleton within the Paviland Cave on the south coast of the Gower peninsula, South Wales. It was covered with red ocher and ceremonially buried with ivory ornaments and perforated seashells, the circumstances hinting at a ritual or shamanic use of the site. Buckland thought it dated from the Roman occupation and called it the Red Lady (319). Note: The skeleton was later identified as Cro-Magnon and dated from roughly 30 K - 20 K years ago; the Early Upper Paleolithic (Early Stone Age).

 

The medical journal The Lancet was founded (1144).

 

1824-1836

James Edward Smith (GB), William Jackson Hooker (GB), and Miles Joseph Berkeley (GB) published their book, The English Flora, to which Berkeley contributed 155 fungus genera, 1,360 species, many of them new (1765).

 

1824

Nicolas Léonard Sadi Carnot (FR) was the first to consider quantitatively the manner in which heat and work are interconnected. He was thus the founder of thermodynamics (339).

 

Hugo von Mohl (DE) provided a clear account of the monocot vascular bundle. In doing so, he concluded that the tissues in the vascular bundles are the same as in dicots; the difference is only in that they are arranged differently (2047; 2052).

 

Thomas Say (US) was an outstanding entomologist, conchologist, and herpetologist who participated in many scientific expeditions and wrote many scientific works. Two of his noteworthy publications are cited (1669; 1670).

 

René-Joachim-Henri Dutrochet (FR) described the movement of vesicular globules (leukocytes) from the vascular lumen to the extravascular space in the transilluminated tadpole tail (596).

Gabriel Andral (FR) and William Addison (GB) reported almost simultaneously the first descriptions of leukocytes; both concluded that the red as well as the white globules of the blood were altered in disease (15; 37). Addison also deduced that pus cells were blood leukocytes that had passed through the wall of capillary vessels.

William Addison (GB) discovered the emigration of polymorphonuclear leukocytes from circulation into tissues, and that this movement increased during inflammation (15; 16).

Gabriel Andral (FR) wrote this first monograph on hematology and one of the most important works in the history of hematology. Here he first uses the terms anemia and hyperanemia and clearly describes a number of diseases of the blood including lead poisoning, septicemia and polycythaemia (37).

Augustus Volnay Waller (FR-GB) independently discovered the emigration of polymorphonuclear leukocytes in inflammation (2078; 2079).

Thomas Wharton Jones (GB) reported for the first time the ameboid movements of leukocytes. Jones differentiated the leukocytes into non-granular, finely granulated, and coarsely granulated types; later to be called lymphocytes, neutrophils, and eosinophils, respectively (981).

Casimir Joseph Davaine (FR) and Nathanael Lieberkühn (DE) observed leukocyte ameboid movement (478; 1118).

Ernst Heinrich Philipp August Haeckel (DE) described the ability of molluscan leukocytes to ingest India ink particles (821).

Maximilian Johann Sigismund Schultze (DE) observed ingestion of particles by mammalian polymorphonuclear leukocyte blood cells (1707).

Albert von Recklinghausen (DE) described granular cells in the frog mesentery; later named mast cells by Paul Ehrlich. He noted translocational locomotion of leukocytes for the first time and observed ingestion of particles by mammalian polymorphonuclear blood cells (2054).

William Peyer (GB) and Maximilian Johann Sigismund Schultze (DE) gave descriptions of translocational locomotion and phagocytosis, which were nearly as complete as any made today (1459; 1708).

Julius Friedrich Cohnheim (DE) showed that the essential feature of inflammation is the passage of white blood cells through the walls of capillaries (diapedesis), and that pus and pus-cells are formed in this way from blood. These experiments traced the migration of stained leukocytes to the center of inflammation in the cornea (400-402). Note: This is the discovery of the origin of pus.

Karl Ewald Konstantin Hering (DE), in 1868, found that leukocytes became adherent to blood vessels near sites of injury (1242).

Christian Albert Theodor Billroth (DE-AT) noted that connective tissue destruction appeared to be associated with leukocyte collections in inflamed sites (161).

Julius Arnold (DE) mentioned that leukocytes pass through specialized openings between endothelial cells via “para-cellular” migration, which he referred to as diapedesis (47).

 

John Goss (GB) observed segregation of a recessive trait in peas but failed to note numerical ratios. In the same year, Alexander Seton (GB) published similar observations (776; 1734).

 

Moritz Herold (DE) was the first to do considerable original work on the embryonic development of a division of the invertebrates—the aranea (spiders) (887).

 

James Scarth Combe (GB) was the first to describe a case of what would later be called pernicious anemia (413).

Thomas Addison (GB) and Hermann Lebert (DE) independently described idiopathic or pernicious anemia based on single cases. Addison called it melasma suprarenale (11-13; 1088).

Michael Anton Biermer (DE) provided the classic description of pernicious anemia (pernicious because no remedy could be found) characterized by anemia, general languor and debility, remarkable feebleness of the heart's action, irritability of the stomach, and a peculiar change of the color in the skin resulting from the deterioration of the adrenal cortex (152; 153).

William Gardner (CA) and William Osler (CA) wrote a thorough and classic account of pernicious anemia (729). Note: This is the first complete account of pernicious anemia and beyond question a major achievement of clinical endocrinology.

George Richards Minot (US) and William Parry Murphy (US) demonstrated that adding liver to their diet could successfully treat patients suffering from pernicious anemia. They suspected that a dietary deficiency of some vitamin was the root cause of the disease (1253; 1319).

William Bosworth Castle (US) and Thomas Hale Ham (US) showed that the substance responsible for preventing pernicious anemia arises from the combination of an intrinsic factor in the gastric juice and an extrinsic factor in the diet. This antianemic factor is stored in the liver (344; 345). Castle called the essential gastric secretion "intrinsic factor" because it is formed in the body.

Lucy Wills (GB) described treatment of pernicious anemia of pregnancy and tropical macrocytic anemia, with special reference to yeast extract (folic acid) as a curative agent (2140).

Tom Douglas Spies (US), Carl F. Vitter (US), Mary B. Koch (US), Margaret H. Caldwell (US) experimentally proved that folic acid from yeast extract is the curative agent for macrocytic anemia (1782).

Lionel Berk (US), William Bosworth Castle (US), Arnold D. Welch (US), Robert W. Heinle (US), Rudolf Anker (US) and Martin Epstein (US) found that absence of gastric juice carrying intrinsic factor would fail to activate and protect vitamin B12 thus leading to pernicious anemia (111).

This intrinsic factor was subsequently found to be a glycoprotein with which vitamin B12 (cyanocobalamin) must combine to be absorbed by the gut. Intrinsic factor is secreted by parietal cells of the gastric glands in the stomach, where it binds with the vitamin. Thus bound, intrinsic factor protects vitamin B12 from digestion as it passes through the gastrointestinal tract. See, Grasbeck, 1965.

Horace Albert Barker (US), Herbert Weissbach (US), and Robert D. Smyth (US) isolated several forms of the B12 coenzyme in pure form and showed that it contained the elements of adenine and a pentose linked to the corrinoid (75).

Mary Shaw Shorb (US) showed that there is a direct relationship between the ability of liver extracts to cure pernicious anemia and their ability to stimulate the growth of the microorganism Lactobacillus lactis Dorner. Thus, a bioassay method was established for what would later prove to be vitamin B12 (1739; 1740).

Edward Lawrence Rickes (US), Norman G. Brink (US), Frank R. Koniuszy (US), Thomas R. Wood (US), and Karl August Folkers (US) were the first to purify, crystallize, and characterize the vitamin B12 (cyanocobalamin) molecule, which surprisingly contains a cyanide group and a cobalt atom (1609). Randolph West (US) clinically verified the ability of the crystalline preparation to arrest Addisonian pernicious anemia (2119).

P. Galen Lenhert (US) and Dorothy Mary Crowfoot Hodgkin (GB) demonstrated by X-ray diffraction analysis that the coenzyme form of B12 was formed by the direct ligation of C-5 of 5-deoxyadenosine to the Co atom at the center of the corrinoid ring of vitamin B12. This was the first demonstration of the existence of a biologically stable and functional carbon-metal bond (1101).

 

D. Schuette (DE) was possibly the first to report use of cod liver oil as a treatment for rickets (wrickken = to twist) (1227; 1704). Note: cod-liver oil had been a folk remedy since time inmemorial.

Francis Glisson (GB), in 1650, recommended rachitis—Greek for spine—as the medical term now used (750).

Pierre Paul Broca (FR) described rickets as a nutritional disease (288).

 

William Prout (GB) discovered that the stomachs of dogs, rabbits, horses, cats, hares, and humans contain free hydrochloric acid (1514).

Friedrich Heinrich Bidder (LV-DE) and Carl Ernst Heinrich Schmidt (LV-DE) proved that the acid of gastric juice is exclusively hydrochloric acid. They quantitatively analyzed gastric juice collected by means of a fistula created in different species of live fasting animals (150).

 

William Frédéric Edwards (GB-FR) studied minimum and maximum temperatures compatible with life; heat production in young animals and adults; resistance of young animals to cold and oxygen deprivation; the importance of humidity, pressure, and movement of air in the loss of heat by transpiration; and the role of light in the development of batrachians (frogs, toads, and newts) (610; 611).

 

Henry Hill Hickman (GB) used carbon dioxide to anesthetize animals prior to surgery and suggested it use on man (343; 895).

 

Jean Civiale (FR), by modifying the primitive lithotrite of Albucasis, introduced a trilable, grasping and fragmenting instrument in 1824. This can be considered the beginning of the use of lithotriptors and ‘endourology’ in stone fragmentation. Henry Bigelow (), in 1874, developed a stronger and harder lithotrite, which was introduced into the bladder with the help of anaesthesia. He filled the bladder, crushed the stones, and evacuated the fragments. This was called litholopaxy. Suddenly, the mortality rate dropped from 25% to 2.4%.

 

William Buckland (GB) published Notice on the Megalosaurus or giant fossil lizard of Stonesfield. This was the first time a dinosaur fossil was described and named (the term dinosaur did not yet exist). In the same science meeting where he described Megalosaurus Buckland also announced the first fossil mammal from the Age of Reptiles (320). Note: In 1822, Gideon Algernon Mantell (GB) found the fossilized tooth of a Cretaceous animal he would characterize as herbivorous, reptilian, and tens of feet long; he would name it Iguanodon. After he prepared a paper reporting Iguanodon for the Royal Society the aforementioned William Buckland urged Mantell to delay, consequently it was Buckland not Mantell who first published on a dinosaur discovery.

 

The Royal Society for the Prevention of Cruelty to Animals (RSPCA) was founded at London, England.

 

The Annales des Sciences Naturelles was founded.

 

1825

Albert Policard (FR) and André Paillot (FR) were among the first to use epi-illumination fluorescence microscopy (vertically incident exciting light) (1486).

Johan Sebastiaan Ploem (NL) developed epi-illumination with narrow band blue and green light (1481; 1482).

Johan Sebastiaan Ploem (NL) and Hans J. Tanke (NL) popularized epi-illumination using fluorescence microscopy (1483).

 

Michael Faraday (GB) reported that benzene reacted with chlorine in sunlight to give a solid body and dense, viscous fluid. This was undoubtedly the first sample of technical benzene hexachloride (BHC). He knew nothing of its biological activity (640).

Teunis van der Linden (NL) was the first to isolate and describe γ-hexachlorcyclohexane (1903). Giovanni Battista Amici The gamma isomer came to be known as lindane in his honor. Lindane (gamma-hexachlorocyclohexane) has been widely used as an antiscabetic and a pediculicidal agent with reasonably good efficacy. Bender mentions the insecticidal properties of hexachlorocyclohexane (HCH) in a patent paper in 1933. HCH was first patented in the 1940s. It should not be confused with benzene hexachloride (BHC), a mixture of various isomers and by-products. Note: Lindane is a nerve poison that interferes with GABA neurotransmitter function.

 

Augustin Pyramus de Candolle (CH) wrote Mémoires sur la Famille des Léguminenses, the beginning of phylogenetic interpretation of this family (501).

 

Robert E. Grant (GB) created the name Porifera "pore-bearer" from (L. porus - pore; ferre - to bear) for the sponges. He began his work in 1825, becoming the first great student of the morphology and physiology of the sponges (787).

 

Henri-Marie Ducrotay de Blaineville (FR) proposed that Malacozoa replace the name Mollusca. Although this was not adopted the term malacologist survived as the name of one who studies mollusks (497).

 

Pierre André Latreille (FR) elevated the batrachians (frogs, toads, and newts) into a separate class, the amphibians (1075).

 

Johannes Evangelista Purkinje; Jan Evangelista Purkyne (CZ) discovered the germinal vesicle (nucleus of the oocyte) in the germ-disk of birds. He was the first to describe the blastula of a fertilized animal egg (bird) (1520; 1522).

Karl Ernst von Baer (EE-DE-RU) discovered the mammalian ovum (1949).

Jean Jacques Marie Cyprien Victor Coste (FR), Jacques Matthieu Delpech (FR), and Thomas Wharton Jones (GB) independently discovered the germinal vesicle (nucleus of the oocyte) in mammals (441; 980).

Rudolph Wagner (DE) discovered an important formation in the ovum of several species of mammals, which he called the macula germinativa – later known as the nucleolus (2068).

 

Martin Heinrich Rathke (DE) discovered embryonic precursors of gills in the embryos of higher animals that lack gills as adults (1555; 1556). He found branchial clefts and branchial arches in the embryos of birds and mammals then worked out the detailed homologies of the gill arches and hyoid in the higher vertebrates. He followed the embryological history of these structures and found that the branchial clefts disappear eventually and that the blood vessels adapt themselves to the lungs. He held that the upper jaw develops from the first visceral arch as a process independent of the lower jaw (1559; 1560; 1562).

Karl Bogislaus Reichert (DE) described the fate of the first and second visceral arches in Anura and Urodela (1584).

 

William Beaumont (US), over a period of several years, observed the responses of the stomach of his famous patient, Alexis St. Martin, who had a permanent fistula into his stomach as the result of a gunshot wound. He extended the work of others on digestion by making clear the physiological responses of the gastric mucosa to chemical and mechanical stimuli. He established the presence of a “chemical principal” in the gastric juice and demonstrated that an empty stomach is devoid of acid; the presence of stomach acid is induced by the presence of food (91; 92; 1148). See, Helm, 1801

 

Caleb Hillier Parry (GB), in 1825, gave one of the earliest descriptions of exophthalmic goiter from a case he observed in 1786 (1420). Note: This and thyromegaly can be symptoms of hyperthyroidism.

Robert James Graves (IE) described a syndrome of palpitation, goiter, and exophthalmos which would later be called Grave's disease (790).

 

Ernst Heinrich Weber (DE) and Wilhelm Eduard Weber (DE) were the first to make a detailed application of hydrodynamic principles to the study of the circulation of the blood (2102).

 

Francois Magendie (FR) later gave an excellent description of cerebrospinal fluid and identified the median aperture (also known as the medial aperture, and foramen of Magendie) which drains cerebrospinal fluid from the fourth ventricle into the cisterna magna (1181; 1182; 1186).

J.-J.-A.-Ernest Faivre (FR) and Hubert Luschka (DE) provided microscopic studies, which gave origin to the hypothesis that the choroid plexuses of the cerebral ventricles elaborate the cerebrospinal fluid (637; 1159).

Auguste Pettit (FR) and Joseph Girard (FR) reported an increase in volume of the choroidal cytoplasm after injections of muscarin, pilocarpine, etc., chemicals known to increase the production of cerebrospinal fluid (1457).

Lewis Hill Weed (US) presented evidence that the perivascular spaces of the central nervous system also pour a small amount of fluid into the subarachnoid space (2104).

Lewis Hill Weed (US) and Harvey Williams Cushing (US) recorded the outflow of cerebrospinal fluid from a catheter inserted into the third ventricle through the aqueduct of Sylvius thus demonstrating an intraventricular source of the fluid (2105).

Walter J. Meek (US) confirmed the work of Pettit and Girard (1232).

Louis Barkhouse Flexner (US) and Hyman Winters (US) found that the cerebrospinal fluid is passed by the cells of the choroid plexuses, which do work in the process, in small amount into the neural cavities (688; 689).

 See, Domenico Felice Antonio Cotugno, 1770

 

Pierre Charles Alexandre Louis (FR) is considered by many to be the founder of medical statistics. He emphasized the careful collection of facts that had been well and completely observed followed by an equally careful analysis of the facts. He called this the numerical method. In his book, Recherches Anatomico-Pathologiques sue la Phthisie, he reported on 123 cases of phthisis (pulmonary tuberculosis) carefully reporting the symptoms, lesions in the various organs, and clinical course of each patient. In his book, Recherches Anatomiques Pathologiques et Therapeutiques sur la Maladie Conne sous les noms de Fièvre Putride, Adynamique, Ataxique, Typhoide, etc., he reported on 138 patients suffering from typhoid using his same careful methodology. In his Recherches sur les Effets de la Saigéee dans Quelques Maladies Inflammatoires, et sur l’Action de l’Emetique et des Vesicatoires dans la Pneumonie, he presented statistical evidence that venesection (bloodletting) never arrested an attack of pneumonia and appeared to have no beneficial effect (1145-1147).

 

Johannes Evangelista Purkinje; Jan Evangelista Purkyne (CZ) discovered what later was called the Purkinje shift or Purkinje effect. Blue objects became brighter than yellow objects when they were placed in dim light. This was later explained, as the rods, which are responsible for vision in dim light, are more sensitive to short wavelengths than to long wavelengths (1521).

 

John Edward Gray (GB) proposed the family name Hominidae for members of the zoological family of man (797).

 

1826-1828

Dengue fever spread from Savannah, Georgia, to other cities along the southeastern coast of the United States, and through the Caribbean (1030).

 

1826-1837

The second cholera pandemic of the 19th century, and the most devastating one, began in Bengal and spread through India in 1826. It reached Afghanistan in 1827 and spread further into central Asia and the Middle East. By late 1830 it had reached Moscow, and from there spread westward into Europe in 1831. It reached England on a ship from Hamburg in October 1831 and spread throughout the British Isles; in England alone 22,000 died. Irish immigrants, fleeing poverty and the potato famine, carried the disease from Europe to North America. On their arrival in the summer of 1832, 1,220 died in Montreal and another thousand across Quebec. The disease then entered the U.S. through Detroit and New York and reached Latin America by 1833. Another outbreak across England and Wales began in 1848, killing 52,000 over two years (1030).

 

1826

René-Joachim-Henri Dutrochet (FR) discovered and named the phenomenon of osmosis, the free passage of water through a membrane that may restrict the movement of substances in solution. He measured osmotic pressure and determined that the pressure related directly to the concentration of dissolved solute. Dutrochet realized that this explained how roots take up water, the movement of water within the plant, and turgor (597-599). See, Jean Antoine Nollet, 1748.

Wilhelm Friedrich Philipp Pfeffer (DE) discovered that if he placed a protein solution inside a semipermeable membrane immersed in water the internal pressure of the bag would rise. The process is osmosis and Pfeffer showed how one might measure the osmotic pressure resulting. He was the first to measure osmotic pressure. Pfeffer also showed that the pressure depended upon the size of the molecules dissolved within the bag. This meant that, from osmotic pressure, the molecular weight of specific proteins could be determined. In this way Pfeffer was able to make the first reasonably reliable measurements of the size of giant molecules (1460).

Burton Edward Livingston (US) gave an excellent presentation of the current state of knowledge regarding diffusion and osmosis in plants (1128).

Per Fredrik Thorkelsson Scholander (SE-NO-US) proposed the solvent tension theory of osmosis (1693).

 

Augustin Sageret (FR) experimentaly crossed muskmelons and cantelopes. He noticed that his parent forms differed from one another in characters that could be contrasted in pairs: flesh white or yellow, seeds white or yellow, flavor acid or sweet. He observed that some of these characters were dominant over others and that the F1 generation was uniform, and he concluded that a hybrid did not show fusion of parental characters, but a 'distribution.' This, however, was not equivalent to a discovery of segregation of unit factors (496; 1645). In this paper Sageret canonized the concept of dominance, the genetic term still in use.

 

Friedrich Tiedemann (DE) and Leopold Gmelin (DE) demonstrated that digestion is, in fact, a complicated series of processes, involving several organs (previously it was thought to be a simple dissolution process). They found that some substances are transformed during digestion, e.g., starch to glucose. They showed that various digestive juices have discrete properties, e.g., secretions of the pancreas can digest proteins, and saliva cannot. They discovered that some biliary substances are purely excretory, e.g., bile pigments, while others play important physiological roles, e.g., absorption of fats. They also discovered shortly after William Prout (GB) and independently of him that the stomach produces hydrochloric acid. Gmelin’s nitric acid test for the presence of bilirubin in chyle, blood serum, and urine is contained here (1856; 1857).

 

Urbain Leblanc (FR) described in cattle what he mistakenly thought was a tumor, so he named it osteosarcoma tumors (lumpy jaw; actinomycosis; oral necrobacillosis; clyers), the most prominent symptoms being swelling and suppuration of the jaw (338; 1089; 1090).

William Dick (GB) treated ‘clyers’ (lumpy jaw, actinomycosis) of cattle with iodine as early as 1839 (25).

Otto Bollinger (DE) described a series of pathologic processes of the jaws and throats of cattle, which had previously been considered as osteosarcoma, bone cancer and wooden tongue. He demonstrated the granulomatous nature of the condition and described detritus containing granulation cells, leukocytes, and opaque, yellow granular bodies, which he regarded as true fungi (196).

Carl Otto Harz (DE) was the first to call the infectious agent ray fungus, or actinomycosis (848).

James A. Cahill, Jr. (US) described actinomycosis in man (338).

James Homer Wright (US) clarified the pathology of actinomycosis (2169).

 

Charles Bell (GB) recognized what he termed a sixth sense, which later was characterized as proprioceptive function (97).

 

Johannes Petrus Müller (DE) showed that sensory nerves could interpret an impulse in but one way, e.g., the optic nerve, however stimulated, records a flash of light whether light is involved or not. In his honor this is called Müller’s law or Muller’s doctrine of specific nerve energies or Müller's law of specific irritability. He discovered the Müllerian duct; worked out the finer structure of glands, bones, and cartilage; described the uriniferous capsule (also described by Bowman); discovered the lymph heart in the frog; discovered the Müllerian duct; and worked out the microscopic anatomy of the glandular and cartilaginous tissues; made a distinction between secretion and excretion. He isolated chondrin and glutin and emphasized, based on his microscopic studies, that there is considerable histological variation among tumors. He started the journal, Archiv für Anatomie und Physiologie, also called Müller’s Archiv. His book, Handbuch der Physiologie des Menschen fur Vorlesungen (Handbook of Human Physiology for Lectures) was monumental (1299-1308). Note: He was an inspiring teacher as witnessed to by his many famous pupils including: Theodor Ambrose Hubert Schwann (DE), Friedrich Gustav Jacob Henle (DE), Emil Heinrich du Bois-Reymond (CH-DE), Karl Vierordt (DE), Rudolph Ludwig Karl Virchow (DE), and Hermann Ludwig Ferdinand von Helmholtz (DE).

 

René-Théophile-Hyacinthe Laënnec (FR) coined the term cirrhosis during a discussion of liver disease, "This type of growth [of the liver] belongs to the group of those which are confused under the name of scirrhus. I believe we ought to designate it with the name of cirrhosis, because of its color. Its development in the liver is one of the most common causes of ascites and has the peculiarity that…the tissue of the liver is absorbed, and it ends often…by disappearing entirely." (1061)

 

Jean-Nicolas Marjolin (FR) first described cancer-like ulcers such as those occasionally seen in chronically irritated or scarred skin. He provided evidence for the involvement of chronic inflammation in the development of cancer. He observed the growth of squamous cancer around the open chronically inflamed wound.

 (1210). In 1850, Robert William Smith (GB), a professor of surgery at Trinity College in Dublin, in his essay Observations upon the Warty Ulcers, first linked Marjolin’s name to the phenomenon he described.

 

Antoine Lembert (FR) introduced a suture, which ensures that during suture of the intestine (enterorrhaphy) serous surface is applied to serous surface when suturing the intestine. This technique laid the foundation of all modern gastric and intestinal surgery (1100).

Johann Friedrich Dieffenbach (DE) was the first to successfully apply Lembert’s suture when he performed the first resection of the small intestine, in a patient with a gangrenous segment of the bowel (527).

Christian Albert Theodor Billroth (DE) performed a number of bowel resections, which are here recounted by Ernst Hauer (DE) (851).

 

Guillaume Dupuytren (FR) gave the first clear pathological description of congenital dislocation of the hip joint. He distinguished this syndrome caused by failure of fetal development of the acetabulum from deformities due to tuberculosis and pyoarthrotic disease of the hip joint (588).

 

Johann Friedrich Dieffenbach (DE) achieved the first surgical closure of the hard palate (524; 525; 1685).

 

Carl Friedrich Benedictus Quittenbaum (DE), in 1826, is credited with the first elective splenectomy. The patient only lived six hours postoperatively (1533; 2114).

Jules Emile Péan (FR) reported the first successful splenectomy. He described his operation on a 20-year-old woman who he believed had a large ovarian or uterine tumor (1436).

Bernard Delaitre (FR), Bruno Maignien (FR), Philippe Icard (FR), Brendan J. Carroll (US), Edward H. Phillips (US), Chester J. Semel (US), Moses J. Fallas (US), and Leon Morgenstern (US) almost simultaneously reported the first laproscopic splenectomy (341; 513).

 

The journal Bijdragen Tot de Natuurku`ndige Wetenschappen was founded.

 

1827-1828

There is an epidemic of acrodynia (epidemic erythema) in France. Acrodynia means pain of the extremities. Karl Petren (SE) notes that the so-called epidemic of acrodynia may have been due to arsenical poisoning. Arsenic was used in the French wine districts for destroying the parasites that attack the grape vines (157; 1453).

 

1827

"I have never yet examined the body of a patient dying with dropsy attended coagulable urine, in whom some obvious derangement was not discovered in the kidneys.... In all the cases in which I have observed the albuminous urine, it has appeared to me that the kidney has itself acted a more important part and has been more deranged both functionally and organically than has generally been imagined." Richard Bright (253)

 

Auguste Arthur Plisson (FR) was the first to isolate aspartic acid. It came from an extract of the root of the marshmallow plant (Althaea officinalis) (1480). Plisson named it.

Karl Heinrich Leopold Ritthausen (DE) described the isolation of proteins from plant seeds in a long series of experiments. He also isolated the long known aspartic acid from hydrolysates of legumin and conglutin (1614; 1615). Aspartic acid is also known as aminosuccinic acid.

 

William Prout (GB) was the first to divide the components of foodstuffs into saccharinous, oleaginous, and albuminous (a historic designation since they correspond to later carbohydrates, fats, and proteins). He recognized fat as an important nutrient in the diet along with protein and carbohydrate. He also determined the composition of urea and lithic acid (uric acid) (1515-1517).

 

Pierre-Jean-François Turpin (FR), an outstanding artist, reports and illustrates his observations of cell division in algae (1890).

 

John James Audubon (FR-US), an artist of birds without peer, began the publication of his four-volume work, The Birds of America (54). This elephant folio is over three feet high and contains 435 hand-colored aquatint plates. This is the most famous of all ornithological works. His five-volume Ornithological Biography followed in 1831. It contains the bird descriptions for The Birds of America (55).

 

Thomas Hodgkin (GB) and Joseph Jackson Lister (GB) described for the first time the true microscopic structure of a range of tissues, e.g., striated muscle and biconcave blood cells. Some science historians consider this paper the origin of animal histology (902).

 

Augustin Sageret (FR) made crosses of the so-called “cantaloupe” and “chaté” varieties of melons, which have different grooves and netting. He studied the combination of these characters in hybrids (1646). Sageret cited unit characters in human eye color and used the term dominant.

 

John Creery Ferguson (IE) was the first in the British Isles to auscultate a fetal heart tone. He had obtained his fetal stethoscope from Jacques Kergeradec (FR) in Paris during 1827.

 

Karl Ernst von Baer (EE-DE-RU) was the first to point out that the ovarian follicle contains the mammalian egg. He made it clear that mammalian development (including man’s) is not fundamentally different from that of other animals. He regarded the sperm cells as entozoa, i.e., parasites, and named them spermatozoa (1949; 1950; 1952; 1953).

 

Francois Magendie (FR) discovered the median aperture in the roof of the fourth ventricle connecting it with the subarachnoid space. This later became known as the foramen of Magendie (1186).

Hubert Luschka (DE) described the paired lateral foramina in the meningeal roof of the fourth ventricle (Luschka’s foramen). They drain the fourth ventricle into the subarachnoid space at the cerebellopontine angle (1160).

 

Nathan Smith (US) gave this early and important description of “necrosis” which today we would call osteomyelitis (1766). Note: In New England at this time it was known as fever-sore.

 

Richard Bright (GB) gave an excellent description of a syndrome characterized by dropsy (edema), sclerosis of the kidney, albuminous urine, cardiac hypertrophy, and an increase in blood urea. In his honor it has been called Bright’s disease (glomerulonephritis) or Morbus Brightii. With nothing more sophisticated than a candle and a silver spoon, he discovered protein in urine, the diagnostic characteristic of Bright's disease of the kidneys. He was the first to suggest a connection between a large heavy heart and contracted kidneys.

Bright also wrote on cerebral lesions, acute yellow atrophy of the liver, nephritis, condensation of the lung in whooping cough, pathologic lesions in typhoid fever, paralysis and tetanus, cerebral hemorrhage, laryngeal phthisis (pulmonary tuberculosis), acute otitis, pancreatic diabetes, unilateral convulsions, and status lymphaticus (202; 250-284).

Frederick Henry Horatio Akbar Mahomed (GB), using a primitive sphygmograph, described high blood pressure (1193). He also linked left ventricular hypertrophy to hypertension due to nephritis and reported the presence of high blood pressure in patients without renal disease (1195; 1196).

Robert M. Kark (ZA-GB-US), Conrad L. Pirani (US), Victor E. Pollak (US), Robert C. Muehrcke (US), and John D. Blainey (US) perfected the technique of renal biopsy in the prone position. They biopsied patients and diagnosed many cases of nephritic syndromes, and toxemia of pregnancy (987).

 

John Rhea Barton (US) performed a femoral osteotomy between the greater and lesser trochanters to secure motion in an ankylosed hip. He performed a subtrochanteric osteotomy of the femur for a severe flexion-adduction deformity of the hip (79). This has been called the first successful arthroplasty.

 

Ludwig van Beethoven (DE) was a 56-year-old man who presented with fever and chills associated with ascites, abdominal pain and a hacking cough with scant hemoptysis. Another dominant feature of his medical history was deafness, which had its onset at the age of 28. Hemoptysis and epistaxis became more frequent and Beethoven eventually developed anuria, became comatose, and died.

Michael S. Donnenberg (US), Michael T. Collins (US), R. Michael Benitez (US), and Philip A. Mackowiak (US) support Beethoven’s cause of death as pneumonia, possibly complicated by bacterial peritonitis; tertiary syphilis with gummatous cirrhosis, luetic otitis, and luetic iridocyclitis (resolved); and irritable bowel syndrome (543; 1174).

Collin S. Karmody (US) and Edgar S. Bachor (US) offered a diagnosis of inflammatory bowel disease (IBD) to provide a single entity that explains most of Beethoven's symptoms and was finally the cause of his death. They concluded that Beethoven's sensorineural hearing loss was an immunopathy associated with IBD (988).

 

Valentine Mott (US) was the first to ligate the common iliac artery at its origin. This was during an operation for aneurysm (1273; 1274).

 

The Medical Gazette was founded in London.

 

The American Journal of the Medical Sciences was founded.

 

The Western Journal of the Medical and Physical Sciences was founded.

 

1828

Robert Brown (GB) reported that under the microscope pollen grains in water, jiggled around irregularly. He tried nonliving particles of about the same size and found the same type of motion. This became known as Brownian movement or Brownian motion (300; 301).

Albert Einstein (CH-US) and Jean Perrin (FR) later supported Brown’s concept (623; 624; 1450). See, Jan Ingen-Housz (Ingenhousz), c. 1790. Note: Brownian motion provided the first incontrovertible evidence of the existence of atoms/molecules. While others had speculated on the existence of atoms, Einstein and Perrin are given credit for being the first to realize that Brownian motion offered evidence for their existence.

 

Friedrich Wöhler (DE) was the first person to accomplish the in vitro synthesis of an organic compound from inorganic compounds. He reacted lead cyanate or silver cyanate with ammonia to produce urea crystals (1966; 2157).

Antoine Béchamp (FR) obtained urea in vitro by protein oxidation (93).

 

Francois-Vincent Raspail (FR), in 1828, noted that plant proteins and sugars together give a purplish-red color when treated with strong sulfuric acid, thus providing the basis of the aldehyde test for the amino-acid tryptophan nearly fifty years before it was introduced (1548).

Francois-Vincent Raspail (FR) applied chemical tests to the protoplasm of Chora, which form the bases of three reactions that are still widely used in the laboratory for the identification of proteins, the xanthoproteic reaction, Liebermann's test and the aldehyde reaction. He tested the reaction of protoplasm with a blue dye obtained from a species of sun spurge, found in the Mediterranean, which turned pink in acid solution (1548; 1549). This anticipated the Voisenet-Fürth reaction.

Count Karl Axel Hampus Mörner (SE) quantified the xanthoproteic reaction (1269).

 

Francois-Vincent Raspail (FR) used hydrochloric acid to detect carbohydrates (furfural or Liebermann reaction). In 1829, he applied the natural acid-base indicator, turnsole, to living cells and determined that the cell interior is acidic. In the same year he proved the presence or absence of various metals in cells by incinerating them and analyzing the ashes and demonstrated by what became known as the xanthoproteic, Liebermann, and aldehyde tests the presence of albuminous matter in the cytoplasm (he called it sap) of plant cells (1550). He is also credited with determining the agent of scabies (the mite Sarcoptes scabiei). See, Giovan Cosimo Bonomo, 1687.

 

Christian Gottfried Ehrenberg (DE) coined the term bacterium as a representative name for some bacterial types. The word comes from the Greek meaning “small stick” (619).

 

Adolphe-Théodore Brongniart (FR) recognized four successive periods of vegetation on earth, each characterized geologically. Three were particularly well characterized: the first, extending to the end of the Carboniferous, dominated by the vascular cryptogams; the third, covering the Jurassic and the Cretaceous, dominated by ferns and the gymnosperms; the fourth, which was the Tertiary, dominated by the dicotyledons.

He also divided the vegetable kingdom into six classes: Agame (thallophytes), cellular cryptogams (liverworts and mosses, i.e., Hepaticae and Muscae), vascular cryptogams, and three classes of phanerogams: gymnosperms, monocotyledonous angiosperms, and dicotyledonous angiosperms.

Adolphe-Théodore Brongniart (FR) made the first attempt at a synthesis of paleobotany: the inventory of fossil genera and the place of these genera in natural classification. He is one of the founders of paleobotany (293; 294).

 

Gottfried Reinhold Treviranus (DE) was the first to accurately draw the rods of the retina, which he called cylinders. He saw these rods in the retinas of birds, mammals, amphibians, and fish. Treviranus associated these structures and their terminal papillae with the optic nerve and with the reception of light (1876; 1878). He incorrectly thought that the rods faced the vitreous chamber.

Friedrich Heinrich Bidder (LV-DE) was the first to recognize the inverted nature of the retinal receptors (147).

Heinrich Müller (DE) identified and numbered the principle layers of the retina (1296; 1297).

Rudolf Albert von Kölliker (DE) described two distinct types of nerve endings in the retina (rods and cones) (2007; 2008).

Maximillian Johann Sigismund Schultze (DE) described the inner and outer segments of the photoreceptors and other elements of the retina, including bipolar cells. He detailed all 10 layers of the retina and proposed that rods are responsible for colorless night vision, whereas cones are necessary for daylight color vision and high-resolution spatial vision. Schultze noted that there is a strong correlation between the peak period of an animal’s activity and the ratio of rods to cones in its retina (1709-1711).

Henri Parinaud (FR) and Augustin Charpentier (FR), independently of Schultze, postulated two classes of photoreceptors (rods and cones), one sensitive to dim light, and the other to daylight. Parinaud wrote that night blindness (hemeralopia) is due to a defect in the rods (363; 1413).

Wilhelm Friedrich Kühne (DE) recognized that stimulation of the cones of the retina mediated the sensation of color while the rods conveyed light or darkness (1046).

Santiago Ramón y Cajal (ES) showed conclusively that in the vertebrate retina there are not only independent receptors and retinal ganglion cells but also bipolar cells between them (1543).

Johannes Adolf von Kries (DE) formulated the modern "duplicity" or "duplexity" theory of vision mediated by rods at low light levels and three types of cones at higher light levels (2014).

 

Karl Ernst von Baer (EE-DE-RU) wrote On Developmental History of Animals, which marks the beginning of modern scientific embryology and contains his strong opposition to preformationism. His publications between 1828 and 1838 described the germ layers of the developing embryo (he thought there were four layers) and how the early stages of the development of vertebrate embryos were quite similar even among creatures that in the end were quite dissimilar. This implies that development proceeds from the sculpturing of general features to the chiseling of fine details, which give the species its distinctive characteristics. He worked out the genesis of all the principal organs from the germ layers. His work led him to conclude that relationships among animals could be deduced more properly by comparing embryos than by comparing adult structure (the origin of comparative embryology). Von Baer was able to show that embryonic notochords appear among vertebrates and some non-vertebrates. This feature-in-common led to their being placed together in the phylum Chordata (1950-1953). See, Pander 1817

 

Karl Ernst von Baer (EE-DE-RU) theorized that, "The eye seems to be an outgrowth of the neural tube, which protrudes through the muscle layer as far as the skin layer, and the outer parts of the eye are changes in the skin evoked as a result." (1951)

Hans Spemann (DE) performed experimental analysis of lens formation in the frog. He cauterized the prospective retina arrangement in the neurula stage of Rana fusca. A few days later, Spemann observed that both the eye and the lens were missing on the operated side of the tadpole. In those cases where the retinal rudiment had not been destroyed, the ability to form lenses appeared to correlate with the ability of the remnant to contact the overlying ectoderm. Spemann claimed that contact of the optic vesicle with the overlying ectoderm was needed to turn that ectoderm into a lens, but he did not know whether it was a sufficient cause. Moreover, he did not know yet whether the optic cup instructed the ectoderm to form a lens or merely acted as a trigger to permit a pre-existing potency to become expressed (1775).

Warren Harmon Lewis (US) provided stringent experimental proof of lens induction following displacement of optic vesicles (which he had pushed caudally underneath the skin). He also achieved heteroplastic lens induction, by placing Rana sylvatica epidermis over the denuded optic vesicle of R. palustris, where it produced a lens (1117).

Helen Dean King (US) found that if the optic cup and the overlying ectoderm are from the same species, lens induction is more likely to be successful (1004).

Ross Granville Harrison (US) obtained experimental induction of the lens by transplanting the optic cup (834).

 

Martin Heinrich Rathke (DE) described the embryonic development of lungs and air sacs in birds (1557).

Armand Juillet (FR) published a comprehensive treatise embracing the anatomical, embryological, histological, and comparative study of the bird's lung (983).

 

Léopold Chrétien Frédéric Dagobert Cuvier (FR) and Achille Valenciennes (FR) wrote Histoire Naturelle des Poissons [Natural History of the Fishes] which summarized and logically organized everything known about fishes up to that time. It included 11,253 pages of text and 650 plates in 22 volumes issued between 1828 and 1849. The work described 4,514 species of fish, 2,311 of these new to science. By studying fish skeletons and internal organs Cuvier separated out spiny-ray finned fishes (acanthopterygians) and realized the importance of whether the pelvis is attached skeletally to the bony structure, not just its relative placement (466).

Charles Alexandre Lesueur (FR) was a student of Cuvier. He made a cabinet of fish dwelling within the Great Lakes and Saint Lawrence River regions.

 

Leon Dufour (FR) established the generic name Gregarina and considered them to be worms (now known to be sporozoans). He observed them in association with insects (571). Filippo Cavolini (IT), in 1787, described a gregarine from the glandular appendages of the stomach of the crustacean Pachygraspus marmoratus Stim. He thought it to be a tapeworm (348).

Rudolf Albert von Kölliker (CH) reported gregarines in the intestine of barnacles and characterized them from the intestine of Balanus pallidus as Gregarina balani. He noted the unicellular nature of gregarines and added considerably to our knowledge of the frequent occurrence and wide distribution of these organisms (2003).

Norman Dion Levine (US) and Virginia Ivens (US) renamed them to the phylum Apicomplexa (1112).

 

James Annersley (GB) wrote, Researches into the Causes, Nature and Treatment of the More Prevalent Diseases of India and of Warm Climates Generally, which is regarded as containing the first accurate descriptions of both intestinal and hepatic amoebiasis (39).

George Budd (GB) made the connection between amoebic dysentery and liver abscesses (322).

Friedrich Alekshandrevitch Lösch; Fedor Lesh (DE) discovered the amoeba, Amoeba coli (Entamoeba histolytica), in 1873 in Russia, and established the relationship between the parasite and the disease in dogs experimentally infected with amoebae from humans (1141; 1142).

Stephanos Kartulis (GR) found Entamoeba histolytica in intestinal ulcers of Egyptian patients suffering from dysentery. He noted that he never found amoebae from nondysenteric cases (993; 994).

Heinrich Hermann Robert Koch (DE) and Georg Theodor August Gaffky (DE) found Entamoeba histolytica in sections of human intestine (1026).

Stephanos Kartulis (GR) also showed that cats could be infected with amoebae per rectum and thus develop dysentery (995).

William Thomas Councilman (US) and Henri Amadée Lafleur (US), while working at the Johns Hopkins Hospital, authored a paper which represented a definitive statement of what was known about the pathology of amoebiasis at the end of the 19th century, and much of it is still valid today (444).

Heinrich Irenaeus Quincke (DE) and Ernst Roos (DE) distinguished Entamoeba histolytica from Enatamoeba coli (1532).

Fritz Richard Schaudinn (DE) was one of the first to show that dysentery can be caused by the amoeba he named Entamoeba histolytica. He distinguished it from the harmless Entamoeba coli (1675).

Alexandre Joseph Emilé Brumpt (FR) proposed two species to distinguish pathogenic and non-pathogenic infections: E. dysenteriae and E. dispar (315).

Based on DNA evidence it has been established that two distinct species exist within what was originally known as Entamoeba histolytica. These are E. dispar and E. histolytica, for the nonpathogenic and pathogenic forms, respectively.

Ernest Linwood Walker (US) and Andrew Watson Sellards (US) demonstrated the infective cyst form of E. histolytica (2073).

 

Thomas Hodgkin (GB) discovered aortic insufficiency (899).

Dominic John Corrigan (GB) described the typical pulse of aortic insufficiency, since commonly known as the Corrigan pulse. He identified it as a sharpely declining "water hammer" pulse (434). Note: This clinical discovery is all the more remarkable because all he had was fingers, a wooden monaural stethoscope, and his great medical acumen.

 

Jean Léonard Marie Poiseuille (FR) demonstrated that blood pressure rises during expiration and falls during inspiration. He also discovered that at each heartbeat the dilatation of an artery is about 1/23 of normal (1484). Poiseuille accurately measured blood pressure using his hemodynamometer. Mercury was layered over potassium carbonate (anticoagulant) in a tube. The end containing the potassium carbonate was connected directly to the blood stream.

 

Jacques Mathieu Delpech (FR) made one of the most significant discoveries of operative orthopedics, namely subcutaneous tenotomy. He also established the tuberculous nature of Pott’s disease, established the true function of the ligaments, and presented a complete list of human physical deformities (514-516).

 

James Syme (GB) performed one of the most remarkable operations of the 19th century. He removed, without anesthesia, a four and one-half pound tumor from the lower jaw of Robert Penman. The patient not only survived the operation, but also remained in excellent health for many years afterwards. The operation was performed with the patient sitting in an ordinary chair, and in all took twenty-four minutes with the loss of seven or eight ounces of blood (1821).

 

Valentine Mott (US) excised the left clavicle for osteosarcoma (1275).

 

S. Pomeroy White (US) successfully ligated the internal iliac artery (2121).

 

John Abercrombie (GB) wrote Pathological and Practical Researches on Diseases of the Brain and Spinal Cord, regarded as the first textbook in neuropathology (6).

 

Boston Medical and Surgical Journal was founded.

 

Glasgow Medical Journal was founded.

 

Lancette Francaise (Gazette des Hopitaux) was founded.

 

1829-1833

In the Pacific Northwest, malaria (the ague) killed an estimated 150,000 Native Americans. Other diseases may have contributed to the death toll, but contemporary writing describes symptoms that closely correspond to those of malaria. The disease was probably introduced in February 1829 by a ship reaching Oregon after coming from Chile, carrying infected mosquitoes in water tanks onboard ship. The Columbia River was flooded at the time, creating stagnant water in which the mosquitoes could breed (1030).

 

1829-1832

During late 1829 an influenza epidemic began in China, then spread from there to the Philippines in September 1830, to Indonesia in January 1831, through the Malay Peninsula and into Asia in 1832. The disease also broke out in Moscow and St. Petersburg in the winter of 1830-31 and spread westward overland through the summer of 1831. By November it had reached the U.S. and continued to spread there in 1832. Another outbreak of influenza spread through Asia and Europe during 1836-37 but except for a single Canadian focus did not reach North America (1030).

 

1829

"Animals have no habits but those that result from the structure of their organs; if the latter varies, they vary in the same manner all their springs of action, all their faculties and all their actions." Étienne Geoffroy Saint-Hilaire (40)

 

Thomas Graham (GB) discovered that the rate of diffusion of a gas is inversely proportional to the square root of its molecular weight. He also showed that the various forms of phosphoric acid differed in their hydrogen content. This introduced chemists to the idea of polybasic acids (782; 783).

 

Louis René Le Canu (FR) isolated and correctly characterized cholesterine (cholesterol) from an extract of hen egg yolk (1081).

 

Johann Friedrich Gustav von Eschscholtz (EE-RU-DE) created the orders Ctenophorae, Discophorae (all medusae), and Siphonophorae (1968).

Von Eschscholtz was one of the first and most important scientists in the exploration of the Pacific, Alaska, and California. Among his publications were the System der Akalephen (1829), and the Zoologischer Atlas (1829–1833). Von Eschscholtz was the first naturalist to describe the acorn worm (Balanoglossus), which he encountered in the Marshall Islands in 1825(1968; 1969). He is commemorated by the plant genus Eschscholzia.

 

Joseph Claude Recamier (FR) coined the term metastasis to describe the transfer of disease from one organ or body area to another to which it is not directly connected (1577).

 

John Stevens Henslow (GB) was a botanist, a vicar of the church, a professor of mineralogy and botany at Cambridge University, and the teacher of Charles Darwin who recommended that Darwin serve as a naturalist aboard H.M.S. Beagle (884; 885). He began teaching at Cambridge in 1826.

 

Martin Heinrich Rathke (DE) found confirmation of the germ-layer theory of Pandar and von Baer in the embryonic development of the invertebrate crayfish (1558).

 

Léon Jean Baptiste Cruveilhier (FR) in his lavish pathological atlases, among other things, described and illustrated cysts, colic diverticulosis, fibrous bodies of the breast, diffuse cerebral sclerosis, gelatinous disease of the peritoneum, dilation of the veins of the abdominal wall, and disseminated (multiple) sclerosis for the first time. He also gave an early description of progressive muscular atrophy, and gastric ulcer (414; 452; 453). His description of enteric ulcer follows: “There is first an erosion of the mucosa…. The erosion or inflammation becomes an ulcer…. Simple ulcer…does not present other than a gross resemblance to cancerous ulcer… The best proof, however, that these ulcerations are not cancerous is their curability…. The [principal] symptoms are…loss of appetite or bizarre appetite, insurmountable distress, difficult digestion…heavy pains in the epigastrium, and sometimes epigastric pain extremely sharp during the process of digestion or indeed when there is no food in the stomach…. The patient has the sensation of an enemy who is always present.”

Friedrich Theodor von Frerichs (DE) provided an exhaustive description of the pathological and clinical findings of multiple sclerosis. He named this affliction brain sclerosis, “hirnsklerose” and was the first to diagnose it in a living patient, which was confirmed by autopsy years later (1974).

 

Gabriel Andral (FR) recognized a direct correlation between the pathology of the blood and that of the organs and tissues. He urged chemical analysis of the blood, especially in morbid conditions. He discussed the role of the blood in plethora, anemia, pyrexia, and so-called organic diseases such as cardiac hypertrophy (36).

 

Michael Sars (NO) and John Graham Dalyell (GB) worked out the life cycle of Aurelia aurita and, more generally, of scyphozoans; demonstrating the relationship between the polyploid and medusoid stages (470; 1664; 1665; 1668).

 

John Vaughan Thompson (IE) published the first records of the vast community of planktonic life. He correctly described barnacles as crustaceans and was the first to describe the planktonic stages of crabs (1834; 1836; 1838; 1840-1842; 1844).

Hermann Burmeister (AR) also saw the larval stages of barnacles and realized that they should be placed with the crustaceans and not with the molluscs (331).

 

Johann Friedrich Georg Christian Martin Lobstein (DE-FR) coined the term arteriosclerosis (1130).

 

Benjamin Guy Babington (GB) invented the laryngoscope, which he called a glottiscope (61). His colleague, W. Thomas Hodgkin (GB), later suggested the name laryngiscope.

Alfred Kirstein (DE) learned of an inadvertent tracheal insertion of an esophagoscope and proceeded to develop a rigid laryngoscope with transmitted light for direct observation. This consisted of a lamp within the handle, focused on a lens and redirected through the scope by a prism (1014).

 

Valentin Hermann Weidenbusch (DE) was the first to synthesized paraldehyde, a central nervous system depressant (2106).

Vincenzo Cervello (IT-GB) was the first to introduce paraldehyde into clinical practice (356).

 

Dominique Jean Larrey (FR) performed the first successful surgery on the pericardium, the patient died within a month (1071).

 

Johann Friedrich Dieffenbach (DE) is considered the founder of modern plastic surgery. He performed the first successful closure of both hard and soft palate; made early references to tenotomy, myotomy, otoplasty, early rhinoplasty, and blepharoplasty. Between 1829 and 1845 Dieffenbach wrote important texts on surgery such as Die Operative Chirurgie (529; 2179).

 

Valentine Mott (US) ligated the carotid during operation for an aneurysm of the arteria innominata (1274; 1276).

Valentine Mott (US) ligated the carotid during operation for anastomosing aneurysm in a three-month old infant (1277).

 

Isidore Geoffroy Saint-Hilaire (FR) suggested that in some apes and man certain infantile characteristics such as large brain and great possibility for adaptation persist into adulthood (1651). This was long before Louis Lodewijk Bolk (NL) enunciated the theory of neoteny. See, Bolk, 1918.

 

Alexandre-Théodore Brongniart, Jr. (FR) named the Jurassic Period in geologic history for the extensive marine limestone exposures of the Jura Mountains, in the region where Germany, France and Switzerland meet (296). Note: Today this represents the middle Jurassic.

 

Jules Pierre Desnoyers (FR) was the first to apply the term Quaternary to a geological time when he discussed Tertiary sediments of the Seine River Valley (he was incorrect) (518).

Henri P.I. Reboul (FR) redefined the period from approximately 2 Ma to the present (1576).

 

James Smithson (GB) donated seed money in his will for the founding of the Smithsonian Institution in Washington, DC.

 

1830-1831

Cholera overruns Russia and invades Western Europe (730).

 

1830

"There is nothing in a caterpillar that tells you it’s going to be a butterfly."Yisrael ben Gedalyah Lipschutz (1124)

 

Charles Lyell (GB) wrote Principles of Geology, a three-volume book on geology, in which he popularized and amplified the Huttonian view that geological change is gradual over eons of time and that the forces for change are the same today as in the past, namely heat and erosion (uniformitarianism). Emphasis on the immensity of geological time was his greatest contribution to or understanding of the earth’s history. It was he who first named the Eocene, Miocene, and Pliocene geological epochs (1162-1164). This new view of the geological past suggested that the question about man’s own antiquity was capable of an empirical answer.

 

David Brewster (GB) discovered the laws governing polarization of light by refraction (249).

 

Joseph Jackson Lister (GB), a London wine merchant, discovered the principle of aplanatic foci then subsequently developed the modern type of compound microscope free of chromatic and spherical aberrations. Only from this time can modern microscopy be said to date. He was the father of Joseph, Lord Lister, the surgeon (1127).

 

Johann Justus von Liebig (DE) discovered the compound he named hippuric (from equine urine) acid. In contrast to benzoic acid it contains nitrogen (2020).

 

Jöns Jakob von Berzelius (SE) was the first to formally define the concept of isomerism. “By isomeric substances I understand those which possess the same chemical composition and the same atomic [molecular] weight, but different properties.” He coined the terms isomer and isomerism (1958). He named the phenomenon isomeria.

 

Louis René Le Canu (FR) was the first to prepare blood hematin (iron protoporphyrin) in pure form. He determined that the iron in blood is associated with the hematin pigment (1082; 1083).

Friedrich Tiedemann (FR) prepared blood hematin (iron protoporphyrin) in crude form (1855). Porphyrin comes from the Greek meaning purple.

Johann Joseph Scherer (DE) described a purple-red iron-free residue that he had extracted from blood using concentrated sulfuric acid (1676).

Gerardus Johannes Mulder; Gerrit Jan Mulder (NL) called Scherer's purple-red extract “iron-free hematin” (1292).

Johann Ludwig Wilhelm Thudichum (DE-GB) recognized the “splendid blood-red” fluorescence of Scherer's hematin, which he purified and called “cruentine” (1848).

Ernst Felix Immanuel Hoppe-Seyler (DE) called the purple substance found in iron-free hematin hemato-porphyrin, in other words “purple-blood” (918; 919).

 

Diederich Franz Leonhard von Schlechtendal (DE), Christian Julius Wilhelm Schiede (DE), Ferdinand Deppe (), and Louis Adelbert von Chamisso (FR-DE) described many of the most important plants of Mexico (2059).

 

William Sharpey (GB) wrote on cilia and ciliary motion. “In the course of some investigations on the development of the tadpole, in which I was lately engaged, I was accidentally led to observe, that the surface of the animal possessed the power of exciting currents of water contiguous to it, in a constant and determinate direction” (1735).

 

John Vaughan Thompson (IE) was the first to recognize the class Polyzoa (1837).

Christian Gottfried Ehrenberg (DE) introduced the name Bryozoa (moss-like animals), which replaced Polyzoa (619).

 

Christian Gottfried Ehrenberg (DE) coined the genus name Euglena in 1830 but it was not diagnosed until 1838 (615; 617).

 

Etienne Geoffroy Saint-Hilaire (FR) originated the concept of parallel evolution in which both the evolution of the phyla and their adaptive convergences are allowed for during classification (1649).

 

Friedrich Schlemm (DE) discovered the corneal nerves (1684).

 

William MacKenzie (GB) presented a classic description of the symptomatology of glaucoma, and was probably the first to draw attention to the increase of intra-ocular pressure as a characteristic of the condition (1173).

 

John Conolly (GB) practiced the doctrines of Philippe Pinel (FR) and introduced the non-restraint treatment of the insane (415). “Restraint and neglect are synonymous. They are a substitute for the thousand attentions needed by a disturbed patient” (416).

 

1831

Samuel Guthrie (US), Eugène Soubeiran (FR), and Johann Justus von Liebig (DE), independently and very near the same time, are all credited with being the first to prepare chloroform. Liebig also synthesized chloral hydrate by chlorinating ethanol (820; 1774; 2022; 2023).

Jean Baptiste André Dumas (FR) determined the composition of and named chloroform (578).

Mathias Eugenius Oscar Liebreich (DE) was the first to use choral hydrate as a sedative-hypnotic (1121). It is infamous as “Knock out drops” or the "Mickey Finn" but has only had legitimate medical use as a sedative in minor surgery and as a topical analgesic (734).

Chloral hydrate, was the first synthetic drug that could truly be called sleep inducing (hypnotic), it depresses the CNS. It became the first commercial sleeping pill but use was discontinued because it irritates the endothelium of blood vessels causing phlebitis. Another negative is that its hypnotic effect is often too long lasting. A Chicago bar owner by the name of Mickey Finn used it to “knock out” rich patrons then rob them.

 

Johann Justus von Liebig (DE) described a novel apparatus for measuring the carbon content of organic materials upon combustion. It was based on trapping carbon dioxide in a five-bulb trap filled with potash (KOH) (2021).

 

Heinrich Wilhelm Ferdinand Wackenroder (DE), in 1831, isolated beta-carotene from carrot roots (Daucus carotus) (2177).

Eduard Schwarz (AT), a ships doctor, knew that night blindness was a recurring problem among sailors on long sea voyages. The wisdom of traditional liver therapy was largely being ignored. On one voyage (1857-1859) he deprived the sailors of liver, 75 of the 352 men developed the condition. Every evening when dusk came, they lost their vision and had to be led about like the blind. Schwartz fed them ox or pork liver and found that the night vision in all the afflicted was restored (1725; 2161).

Richard Martin Willstätter (DE) and Walter Mieg (DE) established the empirical formula of beta-carotene as C40H56 (2141).

Harry Steenbock (US), Erwin G. Gross (US), Paul W. Boutwell (US), and Mariana T. Sell (US) observed that yellow foods are good sources of vitamin A (retinol), whereas white foods and red foods are not (1790-1793).

Elisabeth von Euler-Chelpin (DE), Hans Karl August Simon von Euler-Chelpin (DE-SE), Harry Hellström (SE), and Thomas Moore (GB) offered proof that carotene is provitamin A (1263-1266; 1970).

Paul Karrer (RU-CH), A. Helfenstein (CH), Hansuli Wehrli (CH), and Albert Wettstein (CH) determined the chemical structure of beta-carotene (990).

Paul Karrer (RU-CH), Rudolph Morf (CH), and Kurt Schöpp (CH) worked out the constitutional formula for vitamin A (retinol) and proved that it is related to carotenoids in structure; showing that beta-carotene consists of two vitamin A molecules end to end minus two water molecules (991; 992).

Otto Isler (CH), W. Huber (CH), A. Ronco (CH), and Max Kofler (CH) synthesized vitamin A (966).

Paul Karrer (RU-CH) and Conrad Hans Eugster (CH) synthesized beta-carotene (989).

Hans Herloff Inhoffen (DE), Ferdinand Bohlmann (DE), Käthe Bertram (DE), Günter Rummert (DE), and Horst Pommer (DE) synthesized beta-carotene (963).

Nicholas A. Milas (US), Pauls Davis (), Igor Belic (), and Dragutin A. Fles (HR) synthesized beta-carotene (1249).

John E. Dowling (US) and George Wald (US) reported that the general tissue functions of vitamin A that support growth and maintenance in the rat are served also by vitamin A acid; but since this substance is not reduced, it forms neither the alcohol, the form in which vitamin A is stored, nor the aldehyde (retinene) needed for the synthesis of visual pigments. For this reason, rats maintained on vitamin A acid, though growing normally and otherwise in good condition, become extremely night-blind, and eventually blind. The failure to form visual pigments also has specific anatomical consequences: the outer segments of the visual cells deteriorate, followed by the loss of almost all the cells themselves, in an otherwise normal retina. These anatomical changes resemble those observed in certain hereditary forms of blindness and in human retinitis pigmentosa (546).

Masamitsu Kanai (US), Amiram Raz (US), and DeWitt S. Goodman (US) isolated and characterized retinol-binding protein: the transport protein for vitamin A (retinol) in human plasma (986).

 

Friedrich Tiedemann (DE) and Leopold Gmelin (DE) reported a color test for the presence of protein. They treated the proteins with chlorine water. The proteins had usually been at least partially broken down by harsh treatment (1857).

 

Erhard Friedrich Leuchs (DE) described the hydrolysis of starch by saliva, due to the presence of an enzyme in saliva (ptyalin or salivary amylase) (1107; 1108).

Anselme Payen (FR) and Jean-François Persoz (FR) isolated an amylase complex from germinating barley and named it diastase (1434).

Jöns Jakob von Berzelius (SE) named it ptyalin (to spit) (1960).

Louis Mialhe (FR) purified what he called animal diastase (salivary amylase or alpha amylase) by precipitating it with alcohol (1245; 1246).

 

Alexander Jakulowitsch Danilewsky; Alexander Jakulowitsch Danielewski (RU) separated pancreatic amylase from trypsin (475).

 

Robert Brown (GB) published a paper in England describing the microscopic structure of the reproductive organs of the Orchideae and Asclepiadeae. This study established the nucleus as a constant feature of plant cells and as fundamental unit of cells. It was Brown who coined the term nucleus, meaning little nut. "In each cell of the epidermis of a great part of the family, especially of those with membranous leaves, a single circular areola, generally somewhat more opaque than the membrane of the cell, is observable…only one areola belongs to each cell…This areola, or nucleus of the cell as perhaps it might be termed, is not confined to the epidermis, being also found not only in the pubescence of the surface particularly when jointed, as in Cypripedium, but in many cases in the parenchyma or internal cells of the tissue…The nucleus of the cell is not confined to the Orchideae but is equally manifest in many other Monocotyledonous families; and I have found it, hitherto however in very few cases, in the epidermis of Dicotyledonous plants." (302; 303) Brown is also credited with being among the first to recognize the significance of pollen in fertilization. Note: Franz Julius Ferdinand Meyen (DE) very likely described the nucleus of the cell while examining the algae Spirogyra. If true, this would make Meyen the discoveror of the cell nucleus (1243).

 

Lorenz Oken (DE) reasoned that growth and development of both plants and animals is driven by the multiplication and specialization of the primary vesicles (cells). He states that the process of differentiation between the primary vesicles produces vessels through which sap runs (1371).

 

Marshall Hall (GB) in explaining the distribution of the blood vessels in the tail of the stickleback wrote, “The arteries run immediately along the ray, giving off a few capillary vessels in its course; where the ray divides, the artery gives off a branch to supply the new space formed by this division; at the extremity of the ray, the artery

turns and assumes the character of a vein.” He described for the first time the finer arteriolo-venular communications known in modern terminology as direct channels or thoroughfare channels and reported that in the mesenteric circulation, arteries were seen to give off a minute branch, "and this early to turn around and pursue a venous course." (824; 1066) See, Marcello Malpighi, 1661.

Carl Joseph Eberth (DE), Leopold Auerbach (DE), and Christoph Theodor Aeby (CH) independently published papers which clearly described the cellular nature of capillary walls (18; 57; 606).

 

William Brooke O’Shaughnessy (IE) used intravenous potassium and sodium to successfully treat the loss of these ions in cholera patients (1368).

O’Shaughnessy noted electrolyte imbalance in cholera cases. "The blood drawn in the worst of cases of the cholera is unchanged in its anatomical or globular structure…. It has lost a large proportion of its water, 1000 parts of…serum having but the average of 860 parts of water…. It has lost also a great proportion of its neutral saline ingredients…. Of the free alkali contained in healthy serum, not a particle is present in some…cases, and barely a trace in others…. All the salts deficient in the blood…are present in large quantities in the peculiar white dejected matters." (1367)

Thomas Latta (GB) introduced the practice of intravenous infusion of saline solution to patients suffering from shock associated with cholera. "I at length resolved to throw the fluid immediately into the circulation. In this, having no precedent to direct me, I proceeded with much caution." His first patient was an ‘aged female’, and he used the basilic vein. The result was remarkable, "Ounce after ounce was injected ... when six pints had been injected, she expressed in a firm voice that she was free from all uneasiness" (1076; 1233).

Robert Lewins (GB), Thomas Craigie (GB), and John Macintosh (GB) used the same technique at very near the same date (1113).

William Edmonds Horner (US) found that the rice-water stools in cases of Asiatic cholera consist of epithelium stripped from the small intestine (920; 921).

Frank Macfarlane Burnet (AU), and Joyce D. Stone (AU) reported on how Vibrio cholerae damages the epithelial lining of the gastrointestinal tract (332; 333).

Robert A. Phillips (US) with Taiwanese associates demonstrated that cholera is a disease of extracellular fluid depletion due to reverse sodium transport in the gut. Their treatment of this disease with massive intravenous salt and water repletion resulted in prevention of shock and avoidance of death (1465).

 

Salomon Levi Steinheim (DE) and Jean Baptiste-Hippolyte Dance (FR) were the first to describe the clinical symptoms of tetany (472; 1798).

Francois Remy Lucien Corvisart (FR) coined the term tetanie (tetany) in his thesis (436).

Lothar von Frankl-Hochwart (AT) performed detailed work on tetany then published the first comprehensive monograph dealing with this disease (1972).

 

James Syme (GB) penned a booklet detailing cases where joint excision could be used instead of amputation for diseased joints, as in tuberculosis, and injured joints. In 1842, Syme described an amputation at the ankle. This amputation bears his name, as it replaced a portion of below knee amputations, which were ordinary practice at that time (1822; 1823).

 

Guillaume Dupuytren (FR) described the condition now known as Dupuytren’s contracture (contracture of palmar fascia) and cured it by operation (589; 592).

Felix Platter (CH) was the first to describe flexion contracture deformity of the fingers (Dupuytren’s contracture). This work also contains the first known report of death from hypertrophy of the thymus in an infant (1479).

 

The H.M.S. Beagle put to sea on a cartographic voyage with Charles Robert Darwin (GB) aboard as naturalist.

 

1832

"She had apparently reached the last moments of her earthly existence [when the first injection was given]...The sharpened features, and sunken eyes and fallen jaw, pale and cold, bearing the manifest impress of death's signal began to glow with returning animation…and in the short space of half an hour, when six pints were injected, she was free from all uneasiness…and fancied that all she needed was a little sleep." James L. Gamble recalling Thomas Latta's account in 1832 of a patient and her response to rapid replacement of fluid loss (728). See, Latta in 1831.

 

William Frédéric Edwards (GB-FR) and Jean-Baptiste Marie Baudry de Balzac (DE-FR) were the first to declare that gelatin alone could not replace albuminous bodies in animal nutrition (612). At this time gelatin referred to a substance formed by boiling meat, tendons, etc. with water, rather than the product of deep-seated hydrolysis as would occur with Papin’s digestor.

 

Bartolomeo Bizio (IT) made a study of ‘blood spots’ on communion wafers, and found them to consist of a bacterium, which he named Serratia marcescens. He used bread as a growth medium (175).

 

Barthélemy Charles Joseph Dumortier (BE) was the first to observe cell division in a multicellular organism, Conferva aurea (alga), and to conclude from direct experimental evidence that the growth of the organism was determined by this mechanism alone (581). See, Lorenz Oken, 1831

 

Augustin Pyramus de Candolle (CH) theorized that all plants liberate substances into the soil adjacent to them. These excreted chemicals may be beneficial to some neighboring plants and harmful to others. He observed that the movements of the stem and leaves in response to light produce differential growth effects on their opposite sides and coined the term tropism to connote this movement of a plant towards a source of light (heliotropism) (502). Taxis is often used in reference to tropic behavior in animals.

 

Thomas Nuttall (GB-US) wrote A Manual of the Ornithology of the United States and Canada that was to become the standard text on the subject for most of the 19th century (1366).

 

Étienne Geoffroy Saint-Hilaire (FR) originated modern experimental teratology when he produced abnormal development in chick embryos by varying their environment. These results were reported by his son Isidore Saint-Hilaire and provided an argument against preformation (1652).

 

Johann Lukas Schönlein (DE) and Edward Heinrich Henoch (DE) described allergic non-thrombocytopenic purpura (Schönlein-Henoch purpura) and made the connection between purpura and abdominal pains (883; 1696; 1698). Note: also called purpura rheumatica

William Heberden (GB) had been the first to report this condition (Heberden-Willan disease) (856).

William Osler (CA) was the first to suggest the relationship of the condition to allergy (1380). Note: This syndrome is an allergic reaction to bacteria (especially ß-hemolytic streptococci), food or drugs; it is a form of anaphylactoid (allergic) or non-thrombopenic purpura, which is the most common connective-tissue disorder in children. Schönlein-Henoch purpura is a systemic vasculitis (inflammation of blood vessels) and is characterized by deposition of immune complexes containing the antibody immunoglobulin A (IgA).

 

Thomas Hodgkin (GB) described the malignant lymphoma which bears his name—Hodgkin’s disease (900; 901). See, Marcello Malpighi, 1666.

Samuel Wilks (GB), a fellow physician, also described this lymphoma disease and named it for Hodgkin (2133).

William Osler (CA) mentions chemotherapy for lymphoma (Fowler's solution - arsenic containing medicinal) (1378).

Carl Sternberg (AT) and Dorothy Reed (US) independently described the giant cells associated with Hodgkin’s lymphoma (1579; 1800). They are now called Reed-Sternberg cells.

Paul Chevalier (FR) and Jean Jacques Robert Bernard (FR) gave the first description of the use of high dosage radiotherapy in the treatment of Hodgkin's disease (364).

Louis S. Goodman (US), Maxwell M. Wintrobe (US), William Dameshek (US), Morton J. Goodman (US), Alfred Gilman (US) and Margaret T. McLennan (US) reported on nitrogen mustard therapy. They used methyl-bis(beta-chloroethyl) amine hydrochloride and tris(beta-chloroethyl) amine hydrochloride to treat Hodgkin's disease, lymphosarcoma, leukemia and certain allied and miscellaneous disorders. Then, together with a thoracic surgeon named Gustav Lindskog (US), they injected a less volatile form of mustard gas called mustine (nitrogen mustard) into a patient who had nonHodgkin's lymphoma. The patients tumour masses were significantly reduced for a few weeks after treatment and although the patient had to return to receive more chemotherapy, this marked the beginning of the use of cytotoxic agents for the treatment of cancer (770). Note: This could be the first phase I/II trial on record.

Martin Schneider (US), Edgar J. Poth (US), and William C. Levin (US) found that nitrogen mustard hydrochloride (mechlorethamine) has an antineoplastic effect in Hodgkin's lymphoma (1692).

Emil Frei III (US), Vincent T. DeVita, Jr. (US), John H. Moxley, III (US), Arthur A. Serpick (US), Paul P. Carbone (US), Robert C. Young (US), Bruce A. Chabner (US), Susan P. Hubbard (US), George P. Canellos (US), Brian J. Lewis (US), Dan L. Longo (US), Susan M. Hubbard (US), Margaret N. Wesley (US), Richard I. Fisher (US), Elaine S. Jaffe (US), and Costan Berard (US) made outstanding contributions to the concept of combination therapy in the treatment of Hodgkin’s lymphoma. They demonstrated that MOPP (mechlorethamine, Oncovin [vincristine], procarbazine, and prednisone) chemotherapy could cure advanced Hodgkin's lymphoma (522; 707; 1114; 1134; 2175).

Franziska Jundt (DE), Nina Raetzel (DE), Christine Muller (DE), Cornelis F. Calkhoven (DE), Katharina Kley (DE), Stephen Mathas (DE), Andreas Lietz (DE), Achim Leutz (DE), and Bernd Dorken (DE) determined the mechanism that causes normal B-lymphocytes to mutate into the cancerous cells in Hodgkin's lymphoma (985).

Jason S. Knight (US), Nikhil Sharma (US), and Erle S. Robertson (US) determined the link between Epstein-Barr Virus (EBV) and several cancers including Hodgkin's lymphoma via molecular elimination of the retinoblastoma protein (Rb) (1018).

 

Jean Victor Audouin (FR) and Henri Milne-Edwards (FR) discussed the correlation between the increasing degree of exposure to air from low to high-tide levels and the pattern of distribution of organisms in narrow horizontal zones (53). Today this is referred to as vertical zonation.

 

James Hope (GB) described aortic valve murmurs (915).

 

Johann Friedrich Dieffenbach (DE) was the first to attempt cardiac catheterization on a human, performed during an unsuccessful attempt to obtain blood from a patient suffering from cholera (526).

 

Valentine Mott (US) ligated both carotid arteries simultaneously (at an interval of 15 minutes) during an operation for carcinoma of the parotids. Death occurred within 24 hours with the patient in a state of coma (1278).

 

Guillaume Dupuytren (FR) gave lectures covering the entire surgical panorama of that day, often casuistically disposed with anamnesis, pathological anatomy, differential diagnostics, therapy, postoperative course and unfortunate cases closed with an autopsy report (590; 591).

 

London England experienced an outbreak of cholera followed by two more in 1849 and 1855.

 

Johann Justus von Liebig (DE) established the journal Annalen der Pharmazie, later renamed the Annalen der Chemie und Pharmazie.

 

The Proceedings of the Royal Society of London was founded.

 

1833

"The external world is all-powerful in alteration of the form of organized bodies… these [modifications] are inherited, and they influence all the rest of the organization of the animal, because if these modifications lead to injurious effects, the animals which exhibit them perish and are replaced by others of a somewhat different form, a form changed so as to be adapted to the new environment." Étienne Geoffroy Saint-Hilaire (FR) (1650)

 

Karl Ludwig Reichenbach (DE) discovered creosote (1583).

 

Joseph Louis Gay-Lussac (FR) and Théophile Jules Pelouze (FR) determined the elemental composition of lactic acid (741).

 

Joseph Louis Gay-Lussac (FR) proposed that all plant seeds contain nitrogen (740).

 

Hugo von Mohl (DE) demonstrated that sporangia of pteridophytes (ferns, horsetails, and club-mosses) and the pollen-producing organs of the phanerograms (seed plants) are analogous (2048).

 

Francois-Vincent Raspail (FR) used microincineration to demonstrate that leaves and other parts of plants can be calcined and reduced to ashes, losing all traces of organic matter, without the loss of general structural characters (1551).

Raphael Eduard Liesegang (DE) modified the technique to deal with sections of animal tissues, which were calcined on slides for microchemical analysis (1123).

 

Ferdinand Rose (DE) observed that a green precipitate developed when a protein solution (e.g., egg white or ox serum) was treated with copper sulfate. In the alkaline range, the precipitate became soluble and formed a deep blue-violet color (1625).

Gustav Heinrich W. Wiedemann (DE) showed that the blue-violet color resulting from the reaction of albumins with a solution of alkaline copper sulfate is due to the substance he called biuret (biuret had been identified as a product of the prolonged heating of urea at high temperatures). This is the origin of the famous Biuret Test for proteins (2127).

Karl Heinrich Leopold Ritthausen (DE) and R. Pott (DE) first applied the biuret reaction to the study of proteins (1616).

 

Anselme Payen (FR) and Jean-Francois Persoz (FR) isolated and purified a substance from malt extract, which hastened the conversion of starch to sugar and was destroyed by boiling. They called it diastase (Gk. diastasis, separation). This enzyme, which today we call amylase, was the first enzyme to be purified. Its name set the fashion of using the suffix -ase to name enzymes (1458).

Theodor Ambrose Hubert Schwann (DE) conducted similar experiments in 1836 (1716). These organic catalysts were not yet called enzymes—a name later coined by Wilhelm Friedrich Kühne (DE) (1044). Note from page 190 in Kühne's article: "Um Missverständnissen vorzubeugen und lästige Umschreibungen zu vermeiden schlägt Vortragender vor, die ungeformten oder nicht organisirten Fermente, deren Wirkung ohne Anwesenheit von Organismen und ausserhalb derselben erfolgen kann, als Enzyme zu bezeichnen." Translation : In order to avoid misunderstandings and cumbersome circumlocutions, the presenter proposes to designate as "enzymes" the unformed or not organized ferments, whose action can occur without the presence of organisms and outside of the same.

 

Alfons Wendt (CZ), under the direction of Johannes Evangelista Purkinje; Jan Evangelista Purkyne (CZ), discovered the sudoriferous (sweat) glands of the skin with their excretory ducts (2116).

 

Armand Trousseau (FR) and Amédée Bonnet (FR) reported 200 cases of diphtheria treated with tracheostomy (1882; 1884).

 

William Wallace (IE), in 1833, originally described the disease known variously as lymphogranuloma venereum (LGV), lymphogranuloma inguinale, lymphopathia venerea, Durand-Nicolas-Favre disease, tropical bubo, climatic bubo, strumous bubo, and poradenitis inguinales (2074).

N. Joseph Durand (FR), Joseph Nicolas (FR), and Maurice Favre (FR) defined it as a clinical and pathological entity (593). The causative agent is Chlamydia trachomatis (serologic type L1, L2, or L3).

Wilhelm Siegmund Frei (DE) developed a skin test for lymphogranuloma venereum. It consists of intracutaneous administration of heated pus from an infected bubo, a positive reaction being indicated by the development of an inflamed necrotic area (708).

Sven Curt Alfred Hellerström (SE) and Erik Wassen (SE) transmitted lymphogranuloma venereum to monkeys by intracerebral inoculation (867).

Murray Sanders (US), and Geoffrey Rake (US), Clara M. McKee (US), and Morris F. Schaffer (US) successfully cultivated the agent of lymphogranuloma venereum in tissue culture and chick embryos respectively (1542; 1660).

 

John Hilton (GB) gave the first macroscopic description of parasitic cysts of Trichina in human muscle (897).

Richard Owen (GB) named the organism Trichina spirilis. Louis Joseph Alcide Railliet (FR) later changed the name to Trichinella spirilis (1383; 1541).

Joseph Leidy (US) discovered the cyst of the human parasite Trichina spiralis in pork (1095; 1097).

Rudolf Ludwig Karl Virchow (DE) was the first to describe the adult form of the Trichina worm (1937).

Friedrich Albert Zenker (DE) noted the intestinal and muscular forms of trichinosis and concluded that eating raw pork infected humans (2181).

Karl Georg Friedrich Rudolf Leuckart (DE) and Friedrich Albert Zenker (DE) found that Trichina spiralis causes trichonosis in man (1109; 1110; 2180).

James Paget (GB) and Samuel Wilks (GB) gave the first microscopic description of Trichina cysts in human muscle (1398; 1399).

 

Gilbert Breschet (FR) traced human fetal development from conception until birth. He described embryonic and fetal development, which lead to parturition and birth defects (243).

 

Evory Kennedy (IE) gave the first detailed account of fetal heart tones (1000).

 

Valentine Mott (US) ligated the right subclavian artery within the scaleni during an operation for aneurysm (1279).

 

Carl von Rokitansky (CZ-AT) practiced as a pathologist in Vienna at the Allgemeines Krankenhaus from 1833 to 1875. During this time, he either personally performed or supervised 59,786 autopsies (of which at least 25,000 were medico-legal) reporting the effects of disease on the tissues and organs. This was a novel approach and paved the way for treating patients to halt the anatomical changes occurring during a disease state rather than treating the symptoms, as was common practice. For centuries the accepted method of attempting a cure had been treatment of symptoms, e.g., lower a fever by wrapping the patient in cold towels and placing ice in the mouth.

Rokitansky was one of the first to recognize a relationship between the brain and digestive tract function by pointing out that infectious lesions involving the base of the brain were commonly associated with gastric hemorrhage and perforations of stomach and duodenum. He was the first to differentiate between lobar and lobular pneumonia, gave the first pathological account of spondyloisthesis, the first accurate description of acute yellow atrophy of the liver, and the correct classification of patent ductus arteriosis as a congenital lesion (1694; 2056).

 

1833-1834

A pandemic of influenza occurs in Europe (455).

 

1834 - 1854

André Marie Constant Duméril (FR) and Gabriel Bibron (FR) authored Erpétologie Générale; ou, Histoire Naturelle Complète des Reptiles, a ten-volume work representing a major summary of the field of herpetology. It gives a comprehensive scientific account of the reptiles in general (including the amphibians as the distinct order Batrachia), as to their structure and physiology as well as their systematics, together with an historical account of the literature of the subject (580). Note: Auguste H. A. Dumeril (FR), son of the senior author, aided in the preparation of volumes 7 and 9 after the death of Bibron.

 

1834-1836

There is a dysentery (the "bloody flux") pandemic in Central Europe (3).

 

1834

Michael Faraday (GB) in his report On Electrochemical Decomposition introduced terms suggested to him by William Whewell (GB), and still used today (e.g., anode, cathode, electrolyte, ion, anion, cation) (641; 2138).

 

Friedlieb Ferdinand Runge (DE) obtained carbolic acid (now called phenol) from coal tar (1641).

 

Friedlieb Ferdinand Runge (DE) discovered that coal tar contains several interesting compounds. One of these gave a beautiful bright red color on contact with hydrochloric acid. He called it pyrrole after the Greek word for ‘fiery red oil’ (1642). Aniline blue or its constituents are used to stain collagen as the fiber stain in Masson's trichrome, as well as to reveal callose structures in plant tissues. It can be used in the Mallory's connective tissue stain and Gömöri trichrome stain. It is used in differential staining.

Thomas Anderson (GB) obtained pyrrole in a pure state from ivory oil. He transformed it into its potassium salt then hydrolyzed and distilled it to yield pure pyrrole (35).

Johann Friedrich Wilhelm Adolf Baeyer (DE) and Adolf Emmerling (DE) suggested the structure of pyrrole (67).

Chichester Alexander Bell (GB) and Edwin Lapper (GB) synthesized pyrrole (98).

Ludwig Knorr (DE) and Carl Ludwig Paal (DE) developed synthetic methods for producing derivatives of pyrrole (1019; 1395).

Hans Fischer (DE) and Erich Bartholomäus (DE), Amandus Hahn (DE), W. Zimmerman (DE), Heinrich Röse (DE), Hans Orth (DE), and Bruno Pützer (DE) synthesized naturally occurring pyrrolic pigments (652; 655; 658; 659; 661; 666-668; 670; 672; 676).

Leon Pavel Teodor Marchlewski (PL), in collaboration with Wilhelm Marceli Nencki (PL) proved that hemin and chlorophyll are structurally related (657; 1208). These are the tetrapyrrolic pigments of life.

Hans Fischer (DE) showed that the urine and feces of a case of congenital porphyria, a disease then recently discovered, contained uroporphyrin and coproporphyrin (652; 654; 668).

Hans Fischer (DE) discovered porphyrin syntheses then subsequently synthesized over 130 isomers (652; 655; 670).

Hans Fischer (DE) introduced iron into protoporphyrin thus synthesizing hemin indistinguishable from natural hemin obtained from hemoglobin (656).

Paula Sachs (DE) found that urine from patients with acute porphyria gives a positive Ehrlich reaction, which is characteristic for pyrrole and its derivatives (1644).

Gerry H. Cookson (GB) and Claude Rimington (GB) characterized the component responsible for the positive Ehrlich reaction as porphobilinogen, the precursor of all tetrapyrrolic pigments known (421).

Hans Fischer (DE) developed the techniques necessary to synthesize biliverdin and bilirubin (651; 653; 660; 662-665; 669; 671). Note: In the spleen heme is converted to biliverdin, which is converted to unconjugated bilirubin. Unconjugated bilirubin enters the blood prior to being converted to conjugated bilirubin in the liver. It then enters the small intestine in bile. Conjugated bilirubin is converted to urobilinogen in the small intestine then transported to the kidneys via the blood. In the kidney urobilinogen is converted to urobilin, which is excreted in the urine.

Hans Fischer (DE) put forward his formula for the structure of chlorophyll a. He found that the formula for chlorophyll b is the same as that for chlorophyll a, except that in the former a formyl group replaces the methyl group in pyrrole ring II of the latter (673-675). At his death Fischer had nearly completed the synthesis of chlorophyll. Note: See, Woodward 1960 for the synthesis of chlorophyll.

Hans Fischer (DE) discussed the relationship between hemin and chlorophyll (657).

 

Johann Justus von Liebig (DE) and Eilhardt Mitscherlich (DE) established the elementary composition of uric acid (1254; 2024).

 

Carl Julius Fritsche (DE) correctly described the structure of starch granules (714).

 

Roch-Théogéne Guérin-Varry (FR) fractionated starch from wheat (Triticum spp.) into an integumentary part and two soluble parts, which he called amidon and amylin. When he analyzed these soluble fractions, he discovered that they contained hydrogen and oxygen in the same ratio as occurred in water (812-814).

 

Anselme Payen (FR), in 1834, purified cellulose from plant tissue and named it. The name cellulose started the trend of using the -ose suffix in naming sugars (1431).

 

Ludovici Davidis de Schweinitz; Lewis David von Schweinitz (US) described over 3,000 species of fungi, more than half of which were species new to science (508). De Schweinitz was considered a world authority on the cryptogamia.

 

Christian Gottfried Ehrenberg (DE) classified the cyphonautes larvae of bryozoans as rotifers (616).

A. Schneider (DE) observed the metamorphosis of Electra (1689).

Henri Prouho (FR) presented additional descriptions of cyphonautes (1509).

Jules Barrois (FR) and D.W.J. Vigelius (NL) described coronate larvae of ascophoran bryozoans for the first time (78; 1924).

 

Johann Nepomuk Eberle (DE) showed that an acidic extract of the gastric mucosa causes the dissolution of coagulated egg white (605).

Theodor Ambrose Hubert Schwann (DE) showed that glandular structures in the gastric mucosa of the stomach are responsible for the properties of gastric juice. He also discovered that corrosive sublimate (mercuric chloride) formed a precipitate in such extracts, and that on converting the mercury to its insoluble sulfide and filtering, the filtrate possessed a high degree of digestive power. The active substance thus precipitated he called pepsin (Gk. pepsis, cooking, digestion). This paper constitutes the first mechanistic enzyme study. "Free acid is…essential in digestive action [but is] however not the only active element…. Since [another substance] active in very small amounts carries on the digestion of the most important animal nutrients, one might with justice apply to it the name pepsin." (1716; 1717) See, Beaumont, 1825.

Francois Remy Lucien Corvisart (FR) used pepsin therapeutically to improve digestion (437; 438).

Karl Gotthelf Lehmann (DE), in 1853, gave the name peptone to the end product of pepsin-hydrochloric acid action on proteins (1093).

Rudolf Peter Heinrich Heidenhain (DE) noticed in the stomach three types of cells in the gastric glands and showed that one, the chief or zymogenic cells, secret the enzyme pepsin, a second type the parietal cells secrete hydrochloric acid, and the third type is the epithelial cell (861; 862).

Nikolai Nikolaevich Lubavin (RU-CH-DE) observed that the action of pepsin on proteins results in the uptake of water and cleavage, with the formation of peptones and later leucine and tyrosine (1149).

Joseph Dalton Hooker (GB), Eugen Franz von Gorup-Besánez (AT-DE) and H. Will (DE) concluded that carnivorous plants produce an enzyme like pepsin (911; 1978).

John Howard Northrop (US) was the first to crystallize the enzyme pepsin. He demonstrated that it is a protein (1355; 1356).

Joseph Stewart Fruton (PL-US) and Max Bergman (DE-US) produced a synthetic polypeptide that they cleaved with purified pepsin, thus demonstrating that pepsin hydrolyzes peptide bonds (110; 717).

 

Robert Bentley Todd (IE-GB) defined peripheral neuritis and described the sensory element in sphincter control (1860).

 

Friedrich August von Alberti (DE) first used the term Triassic when he recognized the unity of the three characteristic strata that compose the sedimentary deposits of the Triassic period in Northern Europe. The three-division sequence consists of variegated Bunter sandstone, Muschelkalk (shell) sandstone, and Keuper sandstone (1946). The Triassic Period of the Mesozoic Era was a time of transition and follows the largest extinction event in the history of life, and so is a time when the survivors of that event spread and recolonized.

The organisms of the Triassic can be considered to belong to one of three groups: holdovers from the Permo-Triassic extinction, new groups which flourished briefly, and new groups which went on to dominate the Mesozoic world. The holdovers included the lycophytes, glossopterids, and dicynodonts. While those that went on to dominate the Mesozoic world include modern conifers, cycadeoids, and the dinosaurs.

Rocks rich in Triassic fossils include: the Moenkopi Formation, Arizona; Ischigualasto Badlands, Argentina; Newark Supergroup, Eastern U.S.; Djadochta, Mongolia; and the Chinle Formation, Arizona.

 

The journal Müller’s Archiv (Archiv für Anatomie und Physiologie) was founded.

 

Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences, Paris was founded. It was subdivided into series A, B, and C in 1966.

 

India Journal of Medical Sciences was founded.

 

c. 1835

Ernst Heinrich Weber (DE) discovered that the minimum difference in intensity that the brain can distinguish between two sensations of identical kind maintains a constant relationship to the total intensity of sensation (2100).

 

John Hunter (GB) advised in cases of breast cancer, "We should examine, for example, the glands in the groin or axilla, to ascertain if they are thickened or swoln, and also the course of the absorbents; for if these are sound and movable, then the disease may be safely removed. But if they are deep or fixed as not to admit of removal, then we must consider whether the whole limb can be removed, above the consequent tumours; and if not, we ought to do nothing." In cases of the cancerous testicle Hunter remarks, "but when I recollected that the scrotum was affected, I knew it to be owing to the lymphatics of the scrotum, which pass through the groin." (533; 938) This illustrates his appreciation of lymphatic involvement in malignant disease. Note: John Hunter died in 1793, so the original manuscript was written some time earlier.

 

John Hunter (GB) was the first to clearly describe the pathological details of the formation of sequestra in chronic osteomyelitis. "Exfoliation. —This is the separation of a dead bone from the living, and is not generally understood … When a piece of bone becomes dead, it is then to the animal machine as any other extraneous body and adheres only by the attraction of cohesion to the machine. The first business of the machine, therefore, is to get rid of this cohesion and discharge it …" (938) Note: John Hunter died in 1793, so the original manuscript was written some time earlier.

 

John Hunter (GB) was the first to present a satisfactory theory of the mechanism of referred pain. "This delusion of the senses makes disease seem where it really is not, from the different seat of the symptoms and of the diseased part…When the trunk of the nerve is injured, the pain is referred to the termination of it, as, after amputation of the leg, pain is felt in the toes." (938) Note: John Hunter died in 1793, so the original manuscript was written some time earlier.

Samuel Martyn (GB) discussed the concept of referred pain (1214).

John Hilton (GB) discussed a case for referred pain and described the synovial membrane as a serous membrane endowed with secreting and absorbing powers (898).

 

John Hunter (GB) explained accommodation by the eye. "I saw no power that could adapt the eye to the various distances … unless we suppose the crystalline humour [lens] to be varied in figure." (938) Note: John Hunter died in 1793, so the original manuscript was written some time earlier.

 

John Hunter (GB) observed that the organ of hearing in Mollusks (Cephalopods) is of different construction from that in fishes. He suggested a progression from the invertebrates through the vertebrates, with the Mollusks lacking a semicircular canal, the Cyclostomes having one semicircular canal, the lamprey two, the cartilaginous and bony fishes three (938). Note: John Hunter died in 1793, so the original manuscript was written some time earlier.

 

1835-1839

Typhus fever is epidemic in Scotland (446).

 

1835

Lambert Adolphe Jacques Quetelet (BE) showed the importance of statistical analysis for biologists and laid the foundation of biometry (1530; 1531)

 

Michel-Eugène Chevreul (FR) extracted creatine (Gk. kreas, flesh) from meat (371).

Johann Justus von Liebig (DE), in 1847, confirmed the presence of creatine in the muscle of several animals in and noted that a fox shot during the hunt contained ten times more creatine than a fox living in captivity. He also described the formation of creatinine from creatine (2038).

 

 

Pierre-Jean Robiquet (FR) made the dye rufigallol (412).

Pierre-Joseph Pelletier (FR) refined his procedure for fractioning opium and separated codeine, paramorphine (thebaine), and pseudo-morphine (oxydimorphine, dehydromorphine). The latter was found to be non-poisonous and to contain less carbon and more oxygen than morphine (1440).

 

Johann Justus von Liebig (DE) proved that the first step in the oxidation of alcohol by organisms is the formation of an aldehyde (2025; 2026).

 

Friedrich Gustav Jacob Henle (DE) was among the first authors to use the term cell in its modern biological context. Writing in volume 13 of Encyclopädisches Wörterbuch der Medicinischen Wissenschaften he described the microscopic anatomy of the prismatic epithelium of the gall bladder saying, “If they are turned upward under the microscope, these surfaces appear more or less angular and like cells” (871).

 

Athanase Peltier (FR) and Félix Dujardin (FR) demonstrated that the spermatozoa are produced in the lining of the seminiferous tubules of the testis (573; 574; 1448).

Rudolf Albert von Kölliker (CH), in 1847, demonstrated the true development of the spermatozoa, showing that they are not extraneous bodies, but originate in testicular cells and fertilize the ovum (2002).

 

Hugo von Mohl (DE), for the first time, carefully described some details of mitosis in plants including the appearance of the cell plate between daughter cells. He remarked, "Cell division is everywhere easily and plainly seen in Confervae, Mycelia, Chara and also in terminal buds and root tips of Phanerogams." He described and drew quadripartition by furrowing in the pollen-mother-cells of Cucurbita (2049).

Wilhelm Friedrich Benedikt Hofmeister (DE) used Tradescantia and other species of plants to provide the first accurate descriptions of all stages of mitosis. He was able to describe the loss of the nuclear membrane prior to cell division. He also presented evidence of cell division leading to the formation of tetrads within pollen (meiosis) but did not grasp its significance (906).

Friedrich Anton Schneider (DE) published one of the first reasonably accurate descriptions of indirect cell division which Walther Flemming (DE) would name mitosis. His material was the dividing egg of Mesostomum ehrenbergii, one of the platyhelminths. He observed prophase, metaphase, and the formation of a spindle. He did not name them (1691). Schneider discovered the connection between the adult and larva of phoronids and is commemorated by Spirinia schneideri Villot, 1875 and Protodrilus schneideri Langerhans, 1880) (1690).

Otto Bütschli (DE) and Hermann Fol (CH) independently described complex nuclear changes which occurred during cell division and which are now termed mitosis (335; 699).

Édouard-Gérard Balbiani (FR) was the first to recognize the mechanical significance of the mitotic events. He noted that narrow little sticks (chromosomes) were divided into two groups then partitioned between the two daughter cells (72).

W. Schleiclier (DE) had named this type of cell division karyokinesis (1680; 1681).

Walther Flemming (DE) was a pioneer in applying stains to cells. The nucleus had strong affinity for one of the dyes he used so he called the nuclear material chromatin (Greek, color) and speculated that it might be synonymous with nuclein. When he dyed a section of growing tissue, cells were caught at different stages of cell division and he could sort out the successive stages through which the chromatin material passed. As the process of cell division began, the chromatin coalesced into short threadlike objects that eventually came to be called chromosomes (colored bodies). Because these thread-like chromosomes were so characteristic a feature of cell division, Flemming named the process mitosis, from a Greek word for thread. He chose the epithelial cells of salamander larvae for his studies.

As cell division proceeded, the chromosomes doubled in number. After that came what seemed the crucial step. The chromosomes, entangled in the fine threads of a structure that Flemming named the aster (star), were pulled apart along their length, half going to one end of the cell, half to the other. The cell then divided and the two daughter cells, each left with an equal supply of the chromatin material. And, because of the doubling of the chromosomes before the division, each daughter cell had as much chromatin as the original undivided cell (683-687).

W. Schleiclier (DE), working with frog and cat tissue, was one of the first to describe mitosis in animal cells. He coined the term karyokinesis to describe the nuclear events during mitosis (1680; 1681).

Eduard Adolf Strasburger (PL-DE) accurately described the processes of mitotic cell division and demonstrated that cells are formed directly from previously existing cells (1808; 1809).

Walther Flemming (DE) studied amphibian oocytes, described their giant nucleus or germinal vesicle, and discovered their unusual lampbrush chromosomes, and multiple, peripheral nucleoli. He coined the terms chromatin, mitosis, and spireme (686).

Wilhelm Pfitzner (DE) coined the term chromomeres to name the granules that may appear along a chromosome (1462).

Giulio Cesare Bizzozero (IT), in 1883, made drawings of cells in various stages of mitosis in spleen tissue from the Triton (large sea snail) (1226).

Wilhelm Roux (DE) hypothesized that mitosis is a devise, which ensures that the qualitative properties of the cell nucleus are equally distributed to the two daughter cells. He proposed that each of the chromosomes carries a different genetic load. Roux posited that chromatin grains had distinctly different qualities. Chromatin grains were arranged along chromosomes and each chromosome (Mutterfaden, mother thread) was able to split into two daughter threads or chromatids in present terminology. In this way the two halves of each chromatin grain could be distributed with the help of the spindle apparatus to opposite poles of the cell, irrespective of any possible brisk movements, which might disarrange parts of the filaments, if only it was secured that the filaments were not torn into pieces and dissolved from their centre. Roux further postulated that under normal circumstances two daughter threads resulting from a given mother thread should always be distributed to opposites sites. Otherwise the very reason of the molecular division (Molekularteilung) [of chromatin grains] would be abolished and the latter would become superfluous (1633; 1634).

Eduard Adolf Strasburger (PL-DE) coined the terms prophase, metaphase, and anaphase (1810).

Karl Rabl (DE), from his work with Salamandra maculata und Proteus, theorized that the chromosomes retain their individuality in all stages of the cell cycle. He conjectured that the threads of chromatin into which a given chromosome dissolves when the nucleus enters the resting stage condense again into the same chromosome at the next mitosis. He insisted on constancy in the number of chromosomal filaments characteristic of a given tissue (1534; 1535).

Heinrich Wilhelm Gottfried Waldeyer; Heinrich Wilhelm Gottfried von Waldeyer-Hartz (DE) coined the word chromosome to refer to the chromatin staves (2072). Note: He called them chromosomes because of how well they absorbed chemical dyes under the microscope.

Theodor Boveri (DE) observed that chromatin is the substance that transforms into chromosomes during mitosis (223).

David Paul Hansemann (DE) described in detail the mitotic figures of 13 different carcinoma samples. In every case, he found examples of aberrant mitotic figures. These included multipolar mitoses and anaphase figures that showed asymmetric distribution of 'chromatin loops' (or chromosomes). He postulated that these aberrant cell divisions were responsible for the decreased or increased chromatin content found in cancer cells (1982). This is called the aneuploidy theory of cancer development.

Henrik Lundegardh (SE) coined the term interphase as it applies to mitosis (1158).

Theodor Boveri (DE) suggested that aberrant mitoses lead to the unequal distribution of chromosomes, which, in most cases, are detrimental. Yet, on occasion, a "particular, incorrect combination of chromosomes" will generate a malignant cell endowed with the ability of unlimited growth (schrankenloser vermehrung), which will pass the defect on to its progeny. He foretold the existence of cell-cycle checkpoints (hemmungseinrichtungen), tumor-suppressor genes (teilungshemmende chromosomen) and oncogenes (teilungsfoerdernde chromosomen). He further envisaged that poisons (including nicotine), radiation, physical insults, pathogens, chronic inflammation and tissue repair might all be linked to the development of cancer by indirectly promoting aberrant mitoses or other events that cause chromosome imbalances. Boveri applied his model further to explain the emergence of different tumor types within one tissue, and anticipated the clonal origin of tumors, the allelic loss of recessive chromosome elements, the heritability of cancer susceptibilities, the similarity of the steps that initiate tumorigenesis and those responsible for cancer progression, and the sensitivity of cancer cells to radiotherapy (222; 224).

 

Gottfried Reinhold Treviranus (DE) asserted that spermatozoa are analogous to the pollen of plants (1877).

 

Agostino Bassi (IT), a lawyer by trade and amateur scientist by love, demonstrated that a fungus—later named Botrytis bassiana in his honor—is the causative agent of calcinaccio, a devastating disease of silkworms (the English called it muscardine). This was the first demonstration that an infectious animal disease was due to a microbe. William Bulloch (GB), the historian, says, “He is justly regarded as the real founder of the doctrine of pathogenic microorganisms of vegetable origin" (326).

Bassi also wrote on the use of germicides such as heat, alcohol, acids, alkalies, sulfur, chlorine, calcium chloride, and potassium nitrate. For cholera he recommended immediate isolation of the patient followed by disinfection of their clothes and excreta. When vaccinating children in series he strongly recommended sterilizing the needle between each vaccination to prevent complications or the transference of diseases other than vaccinia (81; 83; 326).

 

Felix Dujardin (FR) associated the sarcode (protoplasm) of protozoa with life processes and placed the foraminiferans among the rhizopods (572).

 

John Vaughn Thompson (IE) published a breakthrough in echinoderm embryology: that stalked pentacrinoids were actually young comatulids (1839).

 

Johannes Evangelista Purkinje; Jan Evangelista Purkyne (CZ) and Gabriel Gustav Valentin (DE-CH) discovered ciliary movements on epithelial cells within the multicellular animal body (1526).

 

Michael Sars (NO) was a pioneer in studies of development, metamorphosis and metagenesis of several marine animal groups, like Scyphozoa, Mollusca, Asteroida, and Annellida. Along with Sven Lovén (SE) he found and described the trochophore larvae (annelid). He was the first to describe the veliger larvae of mollusks (1837, 1840) and the bipinnaria larvae (1835), which he later identified as a stage in development of sea stars (1665-1667).

 

Pierre Charles Alexandre Louis (FR) was the first doctor to question the usefulness of bloodletting. He considered this approach to have had very limited advantages (347).

François- Vincent Raspail (FR) questioned the method in 1845 saying: "But why resort to violent and bloody means? Do you wish to calm fever? You will not succeed by bleeding [ . . . ] So leave your lancet there, it has made enough troubles since Hippocrates." (1553)

 

Jean Baptiste Bouillaud (FR) used experiments as well as controlled clinical and autopsy findings, to confirm the view of Gall, that the center for articulate speech is located in the anterior lobes of the brain. He further pointed out the difference between the ability to create words and the ability to articulate them—internal and external language.

Bouillaud was an outstanding cardiologist, being the first to name and describe accurately the endocardium and endocarditis. He realized that endocarditis usually began with exudation and terminated with cicatrisation (scaring) and deformity of the valves. He pointed out the association between endocarditis and acute articular rheumatism (rheumatic heart disease). See, Baillie, 1793. He elucidated the mechanism and significance of the normal heart sounds, described the positive venous pulse in the neck, stressed its value in the diagnosis of tricuspid insufficiency, and described the bruit du diable, gallop rhythm, the double sound at the apex in mitral stenosis, the friction in pericarditis, extrasystoles, and auricular fibrillation (208-210).

Pierre-Carl-Èdouard Potain (FR) determined that the “Gallop rhythm" [bruit de galop] results from the abruptness with which the dilation of the ventricle takes place during the pre-systolic period…. It appears to be an indirect consequence of the excessive arterial tension which interstitial nephritis produces (1492).

Jean-Baptiste Bouillaud (FR) coined and defined the terms endocarditis and valvular endocarditis (207).

Samuel Wilks (GB) discussed bacterial endocarditis as a source of pyemia in peripheral circulation (2134).

 

James Young Simpson (GB) wrote on diseases of the placenta (1748), peritonitis in the fetus (1750), hernia in the fetus (1751), and hermaphrodism (1749).

 

Wilhelm Friedrich von Ludwig (DE) described Ludwig's angina, otherwise known as angina ludovici; a serious, potentially life-threatening cellulitis or connective tissue infection, of the floor of the mouth, usually occurring in adults with concomitant dental infections and if left untreated, may obstruct the airways, necessitating tracheotomy (2043).

Cellulitis is a diffuse inflammation of connective tissue, caused by bacteria, with severe inflammation of dermal and subcutaneous layers of the skin. Cellulitis can be caused by normal skin flora or by exogenous bacteria. Erysipelas is the term used for a more superficial infection of the dermis and upper subcutaneous layer that presents clinically with a well-defined edge.

 

Jean Zuléma Amussat (FR) presented his method for alleviating hemorrhages of the arteries (34).

 

1836

"The great rule is, to avoid everything which obviously deranges the stomach." Richard Bright (264)

 

Auguste Laurent (FR) advanced the hypothesis that the structural grouping of atoms within molecules determines how the molecules combine in organic reactions (1363).

 

Gerardus Johannes Mulder; Gerrit Jan Mulder (NL), a pioneer in the investigations of molecules associated with living tissue, analyzed silk, egg albumin, serum albumin, blood fibrin, casein, gelatin from several sources, and other substances of similar properties. He concluded that they all contained a common radical which, at the suggestion of von Berzelius, he called protéine, later protein (Gk. proteios, primary) (1282-1287; 1290; 1293). Six years later he wrote, "There is present in plants and animals a substance which … performs an important function in both. It is one of the very complex substances, which under various circumstances may alter their composition and serves … for the regulation of chemical metabolism … It is without doubt the most important of the known components of living matter, and it would appear that, without it, life would not be possible. The substance has been named protein." (1289)

 

Jöns Jakob von Berzelius (SE) demonstrated that the hydrolysis of starch is catalyzed more efficiently by malt diastase than by sulfuric acid and published the first general theory of chemical catalysis (1961-1964). Note: The 1836b paper contains the first definition of catalysis.

Jöns Jakob von Berzelius (SE) coined the term catalysis to name a force involved in the decomposition of hydrogen peroxide. "The catalytic force is reflected in the capacity that some substances have, by their mere presence and not by their own reactivity, to awaken activities that are slumbering in molecules at a given temperature. I shall also call catalysis the decomposition of bodies by this force."(1961-1964)

Jöns Jakob von Berzelius (SE) attributed fermentation to the catalytic force doctrine, which says that yeasts serve as catalysts setting off the fermentation process. Fermentation itself is purely chemical, not requiring living things to carry it out (1962).

 

Charles Giles Bridle Daubeny (GB) investigated the efficiency of different parts of the visible light spectrum in photosynthesis (477).

 

Franz Joseph Andreas Nicolas Unger (AT) proposed the chemical concept of plant distribution, i.e., that mineral content of rocks and soils is the major edaphic influence on substrate-specific plant distribution (1892).

 

Franz Schulze (DE) proved that when a solution containing animal or vegetable matter is boiled, no putrefaction sets in provided that all air, which can have access to the liquid, is previously passed through strong sulfuric acid. Heating the air gives the same result. He also found that boiling would stop a wine fermentation that is in progress. The wine fermentation will remain inactive if air contacting it is treated as above (1712).

 

Asa Gray (US) championed Darwin’s theory of evolution and authored several important botanical works including: Darwinana; Elements of Botany; Flora of North America; How Plants Grow; Field, Forrest, and Garden Botany; and Manual of Botany of the Northern United States (791-796). The Manual of Botany of the Northern United States is now called Gray’s Manual of Botany. It has for many years been the most important and influential book dealing with the Northern American flora.

Merritt Lyndon Fernald (US) authored the eighth edition of Gray's Manual of Botany, the standard for plant identification in North America (648).

 

William Henry Harvey (IE), a great student and popularizer of the algae, was the first to subdivide them into four divisions based on the dominant pigment in their thallus: the Chlorospermeae (green algae), Rhodospermeae (red algae), Melanospermeae (brown algae) and Diatomaceae (diatoms and desmids) (843-847). Today these are called Rhodophyta, Heterokontophyta, Chlorophyta, and Diatomaceae.

Edward Arthur Lionel Batters (GB) presented a systematic correlation of records, extensive distribution mapping, and the development of identification keys for the algae (88).

 

Constantine Samuel Rafinesque; Constantine Samuel Rafinesque-Schmaltz (TR-FR-IT-US), a brilliant eccentric self-taught polymath, published 6,700 binomial names of plants, many of which have priority over more familiar names (189; 1240).

In his Flora Telluriana he details 2,000 new species of plants (1537; 1538).

Rafinesque was the first to declare earthworks, primarily from the Ohio Valley, as the "Ancient Monuments of America." He listed more than 500 such archaeological sites in Ohio and Kentucky (1539; 2096).

 

John Vaughan Thompson (IE) was the first to describe the barnacle, Sacculina, and its parasitism of the shore crab, Carcinus maenas (1843).

 

Alfred Francois Donné (FR), while examining women with abnormal vaginal discharge, discovered and named Trichomonas vaginalis. He at first believed it to be the infectious agent in gonorrhea. He later determined it to be a normal inhabitant of the female genital tract. Donné was, by this work, the first to describe living organisms in pathological conditions, as observed by modern methods (538).

 

John Le Couteur (GB) published a summary of his work on wheat breeding. This summary has been the basis and origin of variety testing (1085).

Patrick Sheriff (GB), c. 1850, improved cereals by deliberately selecting individual ears of great excellence and segregating their progeny from mingling with mediocre stock. He became a celebrated breeder and originator of desirable varieties (512). “A good variety may be safely regarded as the forerunner of a better one” (1738).

 

Richard Bright (GB) and Thomas Addison (GB) gave a very accurate description of appendicitis (284). Appendicitis had been described as early as 30 B.C.E. See, Aulus Cornelius Celsus.

 

John Vaughan Thompson (IE) discovered the Pentacrinus europaeus, and showed that it is the larval form of the feather-star Antedon (Comatula) (1839).

 

Gilbert Breschet (FR) discovered the rete mirabile (marvelous network), the organ that enables whales and dolphins to survive at great depths (244). The rete mirabile exchanges heat, ions, or gases between vessel walls so that the two bloodstreams within the rete maintain a gradient with respect to temperature, or concentration of gases or solutes.

 

Charles Dickens (GB), the novelist, described his character Joe the Fat Boy as loud snoring, hypersomnambulant, obese, with a bizarre personality, polycythemia, and congestive heart failure (523). Doubtless what is today called obstructive sleep apnea syndrome.

Albert G. Bickelmann (US), C. Sydney Burwell (US), Eugene Debs Robin (US), and Robert D. Whaley (US) described a medical case, which they named Pickwickian Syndrome. Their patient, a 51-year-old business executive who stood 5 feet 5 inches and weighed over 260 pounds entered the hospital because of obesity, fatigue, and somnolence (146). The name was chosen because the symptoms are the same as those of the fat boy, Joe, described by Charles Dickens in his The Posthumous Papers of the Pickwick Club, 119 years earlier.

 

Joseph Honoré Simon Beau (FR) was one of the first to characterize myocardial insufficiency and inability of the heart to perform a complete systole. This incomplete contraction involves the ventricles primarily (90).

 

Philippe Frédéric Blandin (FR) wrote the first general treatise on plastic surgery, including a review of the history of plastic surgery (182).

 

Christian Jurgensen Thomsen (DK) studied the characteristics of the tools from different periods of prehistory. Based on the predominant materials of which these tools were made, he divided early human history into the Stone Age, the Bronze (Brass) Age, and the Iron Age (1845). Note: The three-age system originated with Lucretius, however, this work popularized the concept in a practical way.

Thomsen’s work led to the Law of Association, which deduces that objects placed in a grave as part of a burial generally consist of things in use at the time of interment.

John Lubbock (GB) further subdivided the Stone Age into Paleolithic and Neolithic periods (1150). Late in the nineteenth century, the Mesolithic period was conceived.

 

Adam Sedgwick (GB), while working in central Wales, proposed the existence of a separate system below the Silurian, which he named the Cambrian -- commemorating Cambria, the Latin name for Wales (1728). This represents the discovery of the Cambrian Period of the Paleozoic Era. The system was not fully recognized until the faunas were described by Frederick Mac Coy (IE)-AU) and John W. Salter (GB) (1657; 1727).

 

1837-1840

England experiences an epidemic of smallpox (red plague) and typhus fever.

 

1837-1850

An epidemic of cerebrospinal meningitis spreads out over Europe from Bayonne, France.

 

1837

"It is quite impossible for any man to gain information respecting acute disease, unless he watch its progress. Day after day it must be seen; the lapse of eight and-forty hours will so change the face of disease.... Acute disease must be seen at least once a-day by those who wish to learn; in many cases twice a-day will not be too often." Richard Bright. Address delivered at the Commencement of a Course of Lectures on the Practice of Medicine (273).

 

Giovanni Battista Amici (IT) developed very high-powered achromatic lenses for the compound microscope (32). See, Beeldsnijder, 1791.

 

Johann Justus von Liebig (DE) detailed the constitution of organic compounds and described the modern method of chemical analysis. With this book von Liebig introduced the concept of metabolism into physiology. He envisioned his book, titled Die Thier-chemie in later editions, as a complement to his researches into the chemistry of plants. In it he showed, like Lavoisier, that animal heat is not innate, but the result of combustion, introduced the concept of metabolism (Stoffwechsel); and classified animal foodstuffs as fats, carbohydrates and proteins according to their function. He thus became the founder of the modern science of nutrition. Liebig provided one of the first comprehensive pictures of the overall meaning of the ceaseless chemical exchanges which form an integral part of the vital processes (2027; 2031-2033; 2036).

 

Johann Justus von Liebig (DE) showed that aldehyde is an intermediary product in the oxidation of alcohol to acetic acid (2028; 2029).

 

Friedrich Wöhler (DE) and Johann Justus von Liebig (DE) isolated the enzyme emulsin from almond extract (2160).

 

Charles Cagniard-Latour; Cagniard de la Tour (FR) stated that the non-motile globular yeasts of beer are organized bodies probably belonging to the vegetable kingdom. He found that wines contain similar yeast globules which can survive cooling to -5°C in liquid carbon dioxide. He believed that yeast activity formed carbonic acid and alcohol from sugar. Latour observed yeast budding and stated that yeast is a mass of globular bodies capable of reproduction and therefore organized. He stated that yeast is not a simple chemical substance, as had been supposed (336; 337).

 

Theodor Ambrose Hubert Schwann (DE) examined beer yeast with a microscope and described granules, which were often arranged in rows resembling a segmented fungus. He concluded that yeast is without doubt a plant and clearly connected the yeast plant with the fermentation process. He observed that yeast budding, gas development, and fermentation are frequently concurrent events and noted that when the process is at an end the yeast sink to the bottom. He pointed out the differences between the yeasts of beer and wine and spoke of the yeast plant as Zuckerpilz (sugar fungus), from which the term Saccharomyces was later derived. Schwann also noted the requirement of a nitrogenous substance during the yeast fermentation.

He carried out experiments that led him to conclude that putrefaction is brought about by microorganisms (1718-1720).

 

Friedrich Traugott Kützing (DE) described the microbial nature of the scum called mother of vinegar that grows on alcohol, which is being converted to vinegar.

He was clearly of the opinion that yeast was not a chemical substance but a living thing, and in a discussion on the terms organic and inorganic he developed the idea that all fermentation is vital. Kützing was possibly the first investigator to suggest that different fermentations were due to physiologically different organisms (1055).

 

August Carl Joseph Corda (CZ) produced a large body of work on the fungi in which he used the microscope to add thousands of new microscopic species (431).

 

Joseph-Henri Léveillé (FR), Ferdinand Moritz Ascherson (DE), August Carl Joseph Corda (CZ), Johann Friedrich Klotzsch (DE), Philipp Phoebus (DE) and Miles Joseph Berkeley (GB) each independently described the structure of the fungal basidium. Léveille’s paper is best known, for in it he coined the terms basidium and cystidium. Berkeley's 1936 paper introduced the term mycology (49; 112; 113; 116; 431; 1017; 1111; 1466).

 

Friedrich Gustav Jacob Henle (DE) was the first to describe the epithelia of the skin and intestines, defined columnar and ciliated epithelium, and pointed out that this tissue constitutes the true lining membrane of all free surfaces of the body and the inner lining of its tubes and cavities (872).

 

Pierre-François-Olive Rayer (FR) was the first to describe glanders in man (1572). He demonstrated the transmissibility of glanders (a bacterial disease primarily of solipeds) by infecting a horse with material from a case of glanders in a human subject (1573).

Étinne-Guillaume La Fosse (FR) wrote a treatise on the cause of glanders in horses (1057).

Friederich August Johannes Löffler (DE) and Johann Wilhelm Schütz (DE) discovered that the bacterium Pseudomonas mallei (Burkholderia mallei) is the cause of glanders. This is primarily a disease of horses, mules, and the ass (1131).

John W. Nelson (GB), Catherine J. Doherty (GB), Peter H. Brown (GB), Andrew P. Greening (GB), Mary E. Kaufmann (GB), and John R.W. Govan (GB) found that Burkholderia cepacia (Pseudomonas cepacia) is an important pathogen of pulmonary infections in people with cystic fibrosis (CF) (1333).

 

Matthias Jakob Schleiden (DE) published detailed observations on the origin and development of the plant ovule. He confirmed that the pollen tube grows to the ovule and observed that it enters the ovule through the micropyle (1682).

 

René-Joachim-Henri Dutrochet (FR) reported that stomata communicate with lacunae in deeper plant tissue, that only cells containing green pigment can absorb carbon dioxide and transform light energy to chemical energy and demonstrated that mushrooms are in fact the fruiting bodies of the mycelium, detected heat from an individual plant and from an insect muscle (600).

 

Alfred Francois Donné (FR) reported the presence of globules and granular bodies in human milk (539). Some of these were very likely to have been cells.

 

Hugo von Mohl (DE) discovered chloroplasts in plant cells, calling them Chlorophyllkörnern (chlorophyll granules) (1786; 2050; 2053).

 

Theodor Ludwig Wilhelm Bischoff (DE) published a study on respiration, in which he proved that free carbonic acid and oxygen coexisted in blood (169).

 

Louis René Le Canu (FR) found that cholesterol is a constituent of normal human blood (1083; 1084).

 

Ernst Friedrich Burdach; Karl Friedrich Burdach (DE) published a book on microscopic anatomy of the nervous system (330).

 

Heinrich Gustav Magnus (DE) discovered that blood, whether from artery or from vein, contains large amounts of both oxygen and carbon dioxide, that the carbon dioxide released in the lungs is not formed locally by oxidation but has been carried there by the blood, and that more oxygen and less carbon dioxide is contained in arterial than in venous blood. This suggested that carbon dioxide might facilitate the release of oxygen from the blood and that combustion takes place in the capillaries rather than in the lungs (1189; 1192).

Heinrich Gustav Magnus (DE) was the first to postulate that oxygen and carbon dioxide cross the alveolar-capillary membrane of the lungs by diffusion (1190).

 

John Gould (GB) was recruited by Charles Darwin to identify his bird specimens collected during the voyage of the Beagle. It was Gould who identified Darwin's Galapagos finches as separate species, thus providing one of the crucial insights in the development of Darwin's theory. Today these unique finches are known as Darwin's finches or Galapagos finches.

Peter Raymond Grant (GB-CA-US) and Barbara Rosemary Grant (GB-CA-US) spent forty years proving that Charles Darwin did not know the full strength of his theory of evolution. They showed that among the finches of the Galapagos Islands, natural selection sometimes takes place so rapidly we can watch it work (784-786).

Jonathan Weiner (US) wrote The Beak of the Finch: A Story of Evolution in Our Time which described and supported the early work of the Grants (2109).

 

George Newport (GB) reported that there is a correlation between activity and elevated body temperature in a moth, a bumblebee, and a beetle (1335).

Marius Nielsen (DK) showed that human body temperature rises during strenuous activity and is then regulated at a high level corresponding to work output (1342).

Schack August Steenberg Krogh (DK) and Eric Zeuthen (DK) concluded that the temperature of an insect's flight muscle during pre-flight warm-up determines its maximal rate of work output during flight. They demonstrated that wing movements during both pre-flight shivering and flight, are associated with a steady rise in muscle temperature until temperatures approaching human body temperature are reached. The butterfly Venessa could fly when its muscle temperature was as low as 25 degrees C. Their observations of flight muscle temperature in the bumblebee Bombus horti found that the bumblebee's temperatures paralleled those of the butterfly; the thoracic muscles heated up to at least 30°C before flight. In the large lamellicorn beetle, Geotrupes stercorarius, the flight muscles were active even though the wings did not move. When insect subjects stopped exercising they cooled rapidly to their initial body temperatures, and from the cooling curve Krogh and Zeuthen calculated the insects' energy expenditures. They concluded that the heating process during insect pre-flight warm-up is unlikely to be an adaptation for the discharge of nervous impulses from the ganglia to the muscles. Instead, they state that it is "required to allow the muscular engine to develop the energy expenditure for flight" (1040).

 

Isidore Geoffroy Saint-Hillarie (FR), in 1837, lectured at the Athena in Paris on animal behavior. He coined the word ethnologic (ethnology), meaning the study of the relationships of organized beings in the family and society, in the aggregate and the community, and teratology, meaning the study of abnormal individuals (1653). Ethology and ethnology (the study of human groups) are so close in spelling that confusion often arises.

 

Smallpox (red plague) appeared in a Sioux tribe in Missouri, June 1837, then spread to Blackfoot and other tribes in Montana and Saskatchewan. The last previous outbreak among the Blackfoot had been in 1781, so by 1837 most of the population was susceptible (1030).

 

William Lonsdale (GB), in 1837, suggested from a study of the fossils of the South Devon limestones that they would prove to be of an age intermediate between the Carboniferous and Silurian systems. It was Lonsdale who coined the term Devonian, commemorating Devon county, England (1135).

Adam Sedgwick (GB) and Roderick Impey Murchison (GB) presented researches on certain rocks in Devonshire, England, which had a distinctive fossil assemblage that led them to propose a new division of the geological time scale -- the Devonian. Sedgwick and Murchison first used Devonian in a publication (1729). This represents the discovery of the Devonian Period—408 Ma to 360 Ma—of the Paleozoic Era.

 

1838

Jöns Jakob von Berzelius (SE) gave the name organic to substances of living or once-living tissue (1965).

 

Anselme Payen (FR) discovered and determined the chemical composition of cellulose (1431).

Alexandre-Théodore Brongniart, Jr. (FR), Théophile-Jules Pelouze (FR), and Jean-Baptiste-André Dumas (FR), in their report to the French national Academy, coined the terms glucose and cellulose. They defined cellulose as the major constituent of wood, related chemically to starch. They defined it as "... a compound which fills the cells and which makes up the substance of the wood itself." (297) As the group’s recorder, Dumas was most likely the person who coined these words.

 

Jean Baptiste Joseph Dieudonné Boussingault (FR) found evidence that nitrogen enters legume plants by some unknown mechanism, but not the non-legumes (211).

Jean Baptiste Joseph Dieudonné Boussingault (FR) carried out greenhouse experiments, which clearly showed that leguminous plants could fix atmospheric nitrogen (214).

Karl Friedrich Johannes Lachmann (DE) studied the nodules in the roots of legumes and observed bacteria (1059).

Albert B. Frank (DE) showed that the root nodules in legumes are formed due to infection by the organism he named Schinzia leguminosarum believing it to be a fungus (703).

Martinus Willem Beijerinck (NL) isolated symbiotic nitrogen-fixing bacteria (Rhizobium) from root nodules of leguminous plants by using an enrichment culture medium minus nitrogen containing compounds. He speculated but could not prove that these bacteria fixed nitrogen (95).

Albert B. Frank (DE) realized that the infectious agent in the root nodules of legumes is a bacterium. He names it Rhizobium leguminosarum (704).

Perry William Wilson (US), Ervin W. Hopkins (US), Edwin Broun Fred (US), Carl E. Georgi (US), Fred S. Orcutt (US), Miner R. Salmon (US), Joseph C. Burton (US), V.S. Bond (US), Wayne William Umbreit (US), Philip M. West (US), Sylvan B. Lee (US), George Bond (US), and Robert Harza Burris (US) established: 1) growth substance requirements for rhizobia, 2) the effects of carbon dioxide and light intensity on nitrogen fixation, 3) the pN2 function and the pO2 function in nitrogen fixation, 4) the Michaelis constant for nitrogen fixation in Red Clover, 5) that by using heavy nitrogen they could collect data supporting ammonia as a key intermediate in nitrogen fixation, and 6) that hydrogen is a specific and competitive inhibitor of nitrogen fixation in free living Azotobacter vinelandii and in the rhizobia of red clover (334; 706; 745; 2118; 2143-2154).

Robert F. Fisher (US), Thomas T. Egelhoff (US), John T. Mulligan (US), and Sharon Rugel Long (US) discovered that legumes release a flavonoid, which penetrates the rhizobial cells and stimulates a gene-activating protein. The activated bacterial genes produce a Nod factor, which leads to nodule formation by triggering cell division in the legume (680).

Patrice Lerouge (FR), Philippe Roche (FR), Catherine Faucher (FR), Fabienne Maillet (FR), Georges Truchet (FR), Jean Claude Promé (FR), Jean Dénarié (FR), and Frédéric Debelle (FR) purified NodRm-1 (a sulfated beta-1, 4-tetrasaccharide of D-glucosamine), the major alfalfa specific signal from Rhizobium meliloti, which causes root hair deformation and forecasts nodular formation (1104; 1620).

 

Christian Gottfried Ehrenberg (DE) described the first observations of wave-shaped flagella, which he thought were probably necessary for motility (618).

 

George Owen Rees (GB) described a method for isolating sugar from diabetic blood serum (1580).

 

Eugène-Melchior Péligot (FR) determined that the sugar in diabetic urine is grape sugar (glucose) (1438).

 

Johannes Evangelista Purkinje; Jan Evangelista Purkyne (CZ), in 1837, discovered the pear-shaped cells in the cerebellar cortex which bear his name (1523). Note: Tradition has it that Purkinje was the first to use the microtome, Canada balsam, glacial acetic acid, potassium dichromate, and the Drummond limelight in the study of tissues.

 

Theodor Ambrose Hubert Schwann (DE) described ganglion-globules in the gray matter of the brain and spinal cord that contain nuclei and nucleoli, also the double-contoured white substance, which forms the sheath around white nerve fibers (the sheath of Schwann), even noting that the sheath material was fat-like in nature and responsible for the white color of the fibers. It is he for whom the Schwann cells that make up the nerve sheaths are named (1721; 1722; 1724).

 

Christian Gottfried Ehrenberg (DE) proposed that all bacteria be placed in the class Infusoria. He put the study of microorganisms on a systematic basis and was able to establish several groups by clearly recognizing fundamental morphological distinctions, such as those differentiating the spirochetes from certain of the protozoa with which others had grouped them. Some of the names he used, such as bacterium and spirillum, are still used today. It was he who first used the generic term Spirochaeta for the large free-living forms he was observing. He describes the use of indigo and carmine to reveal the stomachs [food vacuoles] of infusorians (617).

 

John Torrey (US), professor of botany at what is now Columbia University, coauthored A Flora of North America with Asa Gray and authored A Flora of the State of New York (1868; 1869). He is commemorated by the genus Torreya (a rare Florida gymnosperm).

 

George Johnston (GB) coined the term hydroida as it applied to animals (977).

 

Robert Remak (PL-DE) mentions that nonmyelinate, sympathetic fibers, "are covered with small, oval or rounded, but more rarely irregular, corpuscles, which exhibit one or more nuclei, in size almost equal to the nuclei of the ganglion globules [nerve cells]," thus most likely referring to "Schwann cells" of nonmyelinated fibers. He suggested that nerve fiber and nerve cell are joined and demonstrated that the neurofibers originated in the ganglionic cells (1589). See, Ross Granville Harrison, 1907.

 

Carlo Matteucci (IT) discovered that the frog’s heart, upon contraction, produces an electric current (1216; 1218).

 

George Gulliver (GB), Friedrich Gustav Jakob Henle (DE), Julius Vogel (DE), and William Hewson (GB) indicated that leukocytes are components of the pus found in suppurating wounds (816; 873; 893; 1942).

 

Johannes Petrus Müller (DE) was the first to describe multinucleate cells in vertebrate tissue. He was studying tumor tissue at the time. Müller was a distinguished physiologist and considered the founder of scientific medicine in Germany (1306).

 

Martin Heinrich Rathke (DE) worked on the embryonic development of the vertebrate skull and among other things found that the two lobes of the pituitary gland have separate embryologic origins. A portion of the roof of the pharynx pushes upward towards the floor of the brain forming the anterior pituitary (adenohypophysis, pars distalis, pars tuberalis, pars intermedia). Where it meets a portion of the brain pushing downward forming the posterior pituitary (neurohypophysis, pars nervosa). Rathke's pouch eventually loses its connection with the pharynx (888; 1564; 1565; 1567).

 

Phillippe Ricord (FR) established syphilis and gonorrhea as separate and distinct diseases. He classified the symptoms of syphilis as 1) Primary symptom (accident primitif), chancre from the direct action of the virus which it produces, and by means of which it propagates itself, 2) Secondary symptoms, or symptoms of general infection, and 3) Tertiary symptoms, (accidents tertiares) occurring at indefinite periods, but generally long after the cessation of the primary affection.

He warns that when gonorrheal matter is in the conjunctive of the eyes one must always insist on quick application of silver nitrate to preserve sight (1610).

 

Jean Etienne Dominique Esquirol (FR) was the first to clinically describe what later became known as Down’s syndrome (633).

Édouard Séguin (FR) also described the Down’s syndrome (1732).

John Langdon Haydon Down (GB) described the syndrome that bears his name, but did not discover its cause (547-550).

Jérôme Jean Louis Marie LeJeune (FR), Marthe Gautier (FR), Raymond Alexandre Turpin (FR), Patricia Ann Jacobs (GB), Albert Gordon Baikie (GB-AU), William Michael Court-Brown (GB), and John A. Strong (GB) were the first to associate a genetic disease in humans with a chromosomal abnormality. They found that Down’s syndrome patients possess an extra small acrocentric chromosome number 21 (972; 1098; 1099). Down’s syndrome is one of the most common causes of mental retardation.

Paul Emanuel Polani (ES-GB), Joseph H. Briggs (GB), Charles Edmund Ford (GB), Constance M. Clarke (GB), and Jeremy M. Berg (GB) discovered a three-generation family with translocation Down syndrome (1485).

 

Edward Selleck Hare (GB), Publio Ciuffini (IT), Henry Khunrath Pancoast (US), and José W. Tobías (AR) described the medical condition later known as Hare’s syndrome or Ciuffini-Pancoast-Tobías syndrome or Pancoast’s disease or superior pulmonary sulcus tumor (830; 1403; 1404; 1859).

 

Karl Wigand Maximilian Jacobi (DE) presented the first formulation of the concept and intent of psychosomatic medicine (an attempt to integrate psychopathology with biology and physiology) (971).

 

Isaac Ray (US) wrote the first treatise on the medical jurisprudence of insanity (1571).

 

Pierre-Francois Verhulst (BE) developed the logistic model of population to describe the self-limiting growth of a biological population (1916; 1917).

Raymond Pearl (US) and Lester James Reed (US) rediscovered the S-shaped logistic curve when they found that human population growth over time seemed to follow this curve (1437).

Vito Volterra (IT) used the logistic equation to construct a nonlinear differential equation model of competition between two species and developed a model of predation in a two-species system, if prey increase, predators will also until prey decrease. As the predators starve, the prey increases. The two populations fluctuate out of phase with each other due to the length of the gestation period delaying the population peaks; i.e., the predator population is still growing after the prey population has begun to decline (1945).

Alfred James Lotka (US) anticipated Volterra with a mathematical model of two-species predation (1143). Today these are known as the Lotka-Volterra equations.

Alexander John Nicholson (AU) and Victor Albert Bailey (AU) tried to improve on the Lotka-Volterra predation model by considering the effects of competition from members of the same species, as well as delays caused by age distribution of the populations (1339).

 

Thomas Bell (GB), Charles Robert Darwin (GB), Thomas Campbell Eyton (GB), George Scharf (GB), and George Robert Waterhouse (GB) published The Zoology of the Voyage of H.M.S. Beagle, Under the Command of Captain Fitzroy, R.N., During the Years 1832 to 1836. : Published With the Approval of the Lords Commissioners of Her Majesty's Treasury (100).

Charles Robert Darwin (GB) published the second edition of The Voyage of the Beagle in 1845 (476).

 

The Great United States Exploring Expedition of 1838–1842 was launched to the Pacific Ocean and surrounding lands. It was of major importance to the growth of science in the United States, in particular the then-young field of oceanography (1789).

 

Jacques Boucher De Crèvecouer De Perthes (FR) found near Abbeville, France, the first evidence of Stone Age man. He determined that they were from the Pleistocene epoch. De Perthes was the first to develop the idea that prehistory could be measured based on periods of geologic time (205; 206).

 

1839

"Every mentally ill person is also physically ill." Johannes Baptista Friedreich (DE) (712)

 

"The elementary parts of all tissues are formed of cells in an analogous, though very diversified manner, so that it may be asserted, that there is one universal principle of development for the elementary parts of organisms, however different, and that this principle is the formation of cells." Theodor Ambrose Hubert Schwann (1722)

 

Michael Faraday (GB) showed that the laws of definite and multiple proportions hold not only for chemical elements, but also for electricity (642).

 

Johann Wolfgang Döbereiner (DE) introduced a test whereby the amount of fermentable sugar present could be determined by the amount of carbon dioxide released by yeast placed in the unknown mixture (532).

 

Anselme Payen (FR) isolated from plants a compound that he recognized as a carbohydrate and which he termed cellulose because it was derived from cell membranes of plants. Payen considered cellulose to be an isomer of starch and dextrine and ascribed the different properties of the three compounds to different states of aggregation (1432; 1433).

 

Pierre-Jean Robiquet (FR) predicted that there is a relationship between an animal’s surface area and its food requirements (1619).

 

Charles Thornton Coathupe (GB) reported that the amount of carbon dioxide in air expired by humans varied between 3.63% and 4.37% (385).

 

Theodor Ambrose Hubert Schwann (DE) introduced the term metabolic (Gk. metabolikon, disposed to cause or suffer change) to denote the chemical transformations undergone by cell constituents and surrounding material (1239).

 

Bernard Rudolf Konrad von Langenbeck (DE) discovered that a yeast-like organism was associated with the white patches of thrush in the mouth, pharynx, and esophagus in a case of typhoid fever (2015). Note: He is perhaps best known today as the "father of the surgical residency."

Fredrik Theodor Berg (SE) recognized the etiological agent of thrush as a mold-like fungus (105).

David Gruby (HU-FR) found Candida albicans in thrush and demonstrated its fungal nature (807).

John Hughes Bennett (GB) reported a cryptogram as the cause of buccal thrush in infants (103).

Fredrik Theodor Berg (SE) independently discovered that a fungus (Candida albicans) causes thrush in man (106; 107).

J. Stuart Wilkinson (GB) described vulvovaginal candidiasis for the first time (2132).

Charles-Philippe Robin (FR) named the causative agent Oïdium albicans (Candida albicans) (1617).

Max B. Burchardt (DE) published experimental, microscopical observations of the thrush fungus. He studied its growth in epithelial preparations on microscope slides over 2 or 3 days and observed septate, branched filaments bearing lateral buds, some filaments growing by the lengthening of terminal buds. These filaments were pseudohyphae, which he described and illustrated (327).

David Haussmann (FR) demonstrated that the causative organism in both vulvovaginal candidiasis and oral candidiasis was Oïdium albicans (Candida albicans) (852; 1166).

Raymond Jacques Adrien Sabouraud (FR) would rediscover Gruby’s findings in 1894.

Christine Marie Berkhout (NL) later proposed the genus name Candida for this yeast-like fungus (118).

 

Johann Justus von Liebig (DE) was convinced that fermentations were of a purely chemical nature and not associated with life forms. His prestige made it difficult for those who espoused that microorganisms caused fermentations to be heard. He demonstrated that the source of animal heat is really the consumption of the fuel taken in through the stomach and lungs (2030).

Johann Justus von Liebig (DE) strongly defends his abiotic chemical explanation for fermentation even in the face of the growing evidence for a biotic explanation (2041; 2042).

Louis Pasteur (FR), in response to von Liebig, brilliantly presses home the case for a biotic theory of fermentation (1428).

Benno Müller-Hill (DE) wrote, "Pasteur was right with his experiments, but Liebig was right with his intuition that fermentation was simple chemistry." (1316)

 

Pierre Sarrus (FR) and Jean-Francois Rameaux (FR) proposed what was called the “surface law”. They suggested that the heat production of different-sized species should be related to their surface area rather than their body masses if they were to maintain the same body temperature (1663). Note: D’Arcy Wentworth Thompson (GB) reminds us, “we are taught by elementary mathematics—and by Archimedes himself—that in similar figures the surface increases as the square, and the volume as the cube, of the linear dimensions.” Thompson then describes the implications of size for both structure and function of animals and plants (1830).

Max Rubner (DE), in 1883, demonstrated the “surface law” in accurate respiration trials on dogs (1636).

Max Kleiber (CH-US) supported the concept that the basal metabolism of animals differing in sex is nearly proportional to their respective body surfaces, i.e., the surface law (1015; 1016).

Victor Regnault (FR) and Jules Reiset (FR) developed an innovative respirometer (one that provided oxygen at the rate it was consumed) and measured the metabolic rates of several animals (including rabbits, dogs, fowl, ducks, finches, sparrows, frogs, salamanders, lizards and beetles). Because their measurements were carried out at temperatures ranging from ~15 °C to ~23 °C (average ~18 °C), and generally on animals feeding, these were not measurements of basal metabolism. However, they did report a number of seminal findings: (i) mammals and birds of the same size had similar mass-specific rates of oxygen consumption; (ii) the mass-specific oxygen consumption of the small bird species (average mass ~23 g) was more than 8-fold greater than the larger birds (average mass ~1.4 kg); and (iii) there was a huge difference in the metabolic rates of the endotherms (mammals and birds) compared to the ectothermic vertebrates (amphibians and reptiles). For example, with respect to this last point, although they were approximately the same size, the mass-specific oxygen consumption rates of the small bird species was ~135 times that of the amphibians and reptiles.

Interestingly, Regnault and Reiset also showed that when they kept rabbits and dogs in an atmosphere that had 2–3 times normal oxygen levels they had the same metabolic rates as those in normal atmospheric oxygen. In other words, metabolic rate seemed to be an intrinsic characteristic of the species and was not limited by the supply of oxygen. In this way it differed from combustion (1582).

Schack August Steenberg Krogh (SE) showed that: (i) the Standard Metabolic Rate of cold-blooded animals (measured at the same temperature as each other) and expressed relative to body mass increased with decreasing body size; and (ii) even when compared at the same temperature that the "oxidative energy of the tissues is greater in the warm-blooded than in the cold-blooded organism." (1039)

 

Martin Heinrich Rathke (DE) described the embryonic development of the snake (1565; 1566).

Martin Heinrich Rathke (DE) and Henry James Clark (US) described the embryonic development of the turtle (378; 1569).

Martin Heinrich Rathke (DE) described the embryonic development of the crocodile (1570).

 

Johann Lukas Schönlein (DE) discovered that favus (honeycomb) of the human scalp, a disease long known, is due to a parasitic fungus (later named Achorion schoenleinii then Trichophyton schoenleinii) growing at the roots of the hair (1697). Note: this was the first infectious disease the etiology of which was scientifically explained. The citation’s title contains the term impetigo, which at this time meant an inflammatory skin disease characterized by isolated pustules.

David Gruby (HU-FR) described the fungi associated with four of the common types of ringworm in man. He conclusively proved for the first time, that a microbial organism could cause disease in man. Culturing the fungus of favus (honeycomb disease) of the scalp he reproduced the disease by reinoculating the fungus into normal areas of the skin, thereby fulfilling what became famous forty years later as Koch’s postulates, in the study of anthrax (805; 806). This experiment proved, for the first time, that a microorganism was the cause of a human disease. See, Schönlein, 1839.

Robert Remak (PL-DE) cultured the etiologic agent of favus on apple slices, induced the infection in himself and validly described it as Achorion schoenleinii, in honor of his colleague Schönlein (1593).

 

Johann Lukas Schönlein (DE) is also attributed with naming the disease, Tuberculosis (1697). Prior to Schönlein's designation, Tuberculosis had been called "consumption".

 

Jean Baptiste Joseph Dieudonné Boussingault (FR) and Louis Frédéric Le Bel (FR) quantitatively studied the balance between the elementary constitution of the maintenance ration of a cow and that of the excretions and the milk (221).

 

Francois Magendie (FR) was possibly the first experimenter to induce anaphylaxis. He found that rabbits that tolerated an initial injection of egg albumin often died upon receiving a second injection (1184).

 

Alfred-Armand-Louis-Marie Velpeau (FR) described hidradenitis suppurativa, a chronic acneiform infection of the cutaneous apocrine glands that also can involve adjacent subcutaneous tissue and fascia (1914).

 

Bogislaus Palicki (CZ), in his dissertation directed by Johannes Evangelista Purkinje (Jan Evangelista Purkyne) (CZ), discovered Purkinje fibers in heart muscle (1400; 1401).

Wilhelm Kaspar (CZ), in 1839, in his dissertation directed by Johannes Evangelista Purkinje (Jan Evangelista Purkyne) (CZ), discovered Purkinje fibers in the wall of the uterus (996).

Johannes Evangelista Purkinje (CZ) reports the discovery of Purkinje fibers (1525).

 

Pierre-François-Olive Rayer (FR) is recognized as the "father" of renal microscopy. It was probably Rayer who first used the term Bright's disease (maladie de Bright) (1574).

 

James Hope (GB) described mitral regurgitation and discussed why it is undesirable. He also discussed how to diagnose pulmonary stenosis (916).

 

Isaac E. Taylor (US) and James Augustus Washington (US), in 1839, administered a solution of morphine in an Anel syringe. This was one of the earliest subcutaneous injections of an anesthetic (42).

 

Eduard Caspar Jacob von Siebold (DE) wrote the first and arguably the most important history of obstetrics (2060).

 

Samuel David Gross (US), an outstanding surgeon, wrote Elements of Pathology and Anatomy, the first exhaustive treatise on pathological anatomy in the English language. In 1854, he wrote A Practical Treatise on Foreign Bodies in the Air-Passages, the first systematic treatise on this subject and, in 1859, finished System of Surgery, published in two volumes totaling 2,360 pages. Gross became world famous being honored by Oxford University with the D.C.L. degree and Cambridge University with the L.L.D. degree (802-804).

 

Richard Owen (GB) announced one of his most important discoveries: his identification of the bones of a giant bird from New Zealand. The Moa now ranks among the world's most famous extinct birds (1384).

 

Roderick Impey Murchison (GB), while studying the rocks of Wales, documented rock strata containing a distinctive set of fossils, one in which very few fish were found, but that included numerous different types of trilobites, brachiopods, and other such fossils. Murchison named the system of rocks containing such fossils the Silurian, commemorating the Silures, a Celtic tribe living in the Welsh Borderlands at the time of the Romans (1317). This represents the discovery of the Silurian Period of the Paleozoic Era.

 

1840-1849

There was a pandemic of cholera.

 

c. 1840

Sam Shuster (GB), from an analysis of original correspondence, established that Karl Marx's incapacitating skin disease was hidradenitis suppurativa, not 'boils' as was universally assumed at the time and since. Shuster says, "The psychological effect of this illness on the man and his work appears to have been considerable …In addition to reducing his ability to work, which contributed to his depressing poverty, hidradenitis greatly reduced his self-esteem." (1741)

 

1840

“Race after race resigned their fleeting breath— The rocks alone their curious annals save.” Timothy Abbott Conrad (417)

 

Germain Henri Hess (CH-RU) measured the heats evolved in various reactions and was able to demonstrate that the quantity of heat produced in going from substance A to substance B was the same no matter by what chemical route the reaction proceeded or in how many stages (892).

 

Johann Justus von Liebig's Thierchemie which united the fields of chemistry and physiology was published (2032).

 

Christian Friedrich Schönbein (CH) discovered and named ozone (Gk. ozein, to smell). He found that it forms during electrical discharges (1695).

Sydney Chapman (GB) published the theory of ozone formation and depletion. The reactions are still valid and called the 'Chapman cycle' or the 'Chapman reactions'. Oxygen and ozone are transformed into each other. The bonds are broken by photolysis due to solar radiation. To break the bond in O2 the energy of the sunlight must be higher (wavelength shorter than 240 nm), than for ozone (wavelength shorter than 900 nm). Formation and depletion are in equilibrium and the net result is a 'zero' reaction: Oxygen absorbs in the highly energetic UV-C range, ozone in the slightly less energetic UV-B range. Longer wavelengths partially pass the atmosphere and reach the Earth surface (359).

Richard S. Stolarski (US) and Ralph J. Cicerone (US) proposed that chlorine coming from rocket fuel in supersonic transport (SST) aircrafts could destroy ozone (1807).

Mario J. Molina (MX-US) and F. Sherwood Rowland (US) published an article highlighting the threat to the ozone layer posed by chlorofluorocarbons (CFCs) used as refrigerants, aerosol sprays, and in making plastic foams (1262).

Joseph C. Farman (GB), Brian G. Gardiner (GB), and Jonathan D. Shanklin (GB) reported a 40% decrease in ozone over the Antarctic between 1977 and 1984 (643).

 

Johann Justus von Liebig (DE) and Friedrich Wöhler (DE) attributed decomposition, eremacausis (Gk. erema, quietly + causis, burning), putrefaction, and fermentation to chemical instability of certain substances which were able to communicate their instability to other substances in succession. Liebig asserted that animalcules are the result, not the cause of fermentation and further that "if agitated in a vessel filled with a solution of sugar the molecules of yeast communicate their condition to the particles of sugar, the result being the formation of alcohol and carbon dioxide, compounds in which the constituents are retained in combination with a greater force than in sugar. In contact with sugar, yeast disappears, and none is reproduced, but when added to the gluten (albumin) contained in vegetable juices new yeast is formed. Yeast, therefore, is produced from gluten."

Johann Justus von Liebig (DE) introduced the concept of metabolism and was the first to realize that the addition of a single fertilizer will increase crop yield only if a particular soil can deliver all the other necessary nutrients. Depending on the circumstances, therefore, any of the essential minerals (nitrates, phosphates, potassium, etc.) might become the controlling factor. This generalization became known as Liebig's "Law of the Minimum" and it remains today a central concept in agriculture (2031). Note: Thanks to Felicjan Odrowąż Sypniewski's (PL-DE) notes and observations Karl Phillip Sprengel (DE) formulated his "Theorem of Minimum." Johann Justus von Liebig, who later popularized this theorem, was mistakenly attributed to its authorship (1785)).

 

Jean Louis Rodolphe Agassiz (CH-US) wrote a large five-volume work on fossil fishes. He was the first to conclude—from his studies of glaciers— that there had been an Ice Age. He borrowed the phrase ice age (Eiszeit) from a poem by his friend Karl Schimper (21-23; 1209). He is commemorated by Phascolosoma agassizii Keferstein, 1867, Polydora agassizi Claparède, 1969, Linvillea agassizi (McCrady, 1857), and Aglauropsis agassizi Fr. Müller, 1865.

James Croll (GB) was the first to suggest that variations in the Earth’s orbit might have initiated ice ages (450).

Milutin Milankovitch; Milutin Milankovic (Serbian), in 1930, found that for Croll’s theory to be a more accurate predictor of ice ages it must consider that over long periods of time the Earth tilts, pitches, and wobbles relative to the Sun (1248).

 

Johannes Evangelista Purkinje; Jan Evangelista Purkyne (CZ) was one of the first to use a mechanical microtome to prepare thin sections of tissue for microscopic analysis, introducing glacial acetic acid, potassium dichromate and Canada balsam in the preparation of tissue samples for microscopic examination. He was aware of the cellular nature of the skin and other animal organs. He coined the word protoplasm in reference to the living embryonic material in the egg and together with Hugo von Mohl (DE) who applied the term protoplasm to the living content of plant cells, established the protoplasm concept (1524; 1817; 2051).

Ferdinand Julius Cohn (DE) unified the concept of protoplasm by applying it equally to both plant and animal cells (392).

 

René-Joachim-Henri Dutrochet (FR) detected heat production by a plant and by an insect’s muscles during movement (601).

 

Friedrich Gustav Jacob Henle (DE), one of the most celebrated anatomists of the world and the greatest histologist of his time, was one of Heinrich Hermann Robert Koch’s teachers. He wrote, Von den Miasmen und Contagien und von den Miasmatisch-Contagiösen Krankheiten (On Miasmas and Contagions and Concerning Miasmatic-Contagious Diseases), a monograph in which he lays the foundation for Koch’s postulates and stated, "The material of contagions is not only organic but a living one and is indeed endowed with a life of its own, which is, in relation to the diseased body, a parasitic organism." He insisted that in order to claim a given microbe caused a disease (1) there be constancy in the association of a given disease and its supposed parasitic cause, as well as its absence in other diseases, (2) isolation and separation from other microbes, and finally, (3) proof of the power of the isolated germ to produce disease (880).

He was the first to describe the epithelium of the skin and intestines, to define columnar and ciliated epithelium, and to point out the importance of epithelium as the lining membrane of all free surfaces of the body and of its tubes and cavities. His Allgemeine Anatomie was the first systematic treatise on microscopic anatomy. In it, for the first time, he described the presence of smooth muscle in the endothelial lining of small arteries, a finding critical to the later understanding of the vasomotor mechanism. The 1866-1871 citation contains the first logical account and nomenclature of the axes and planes of the body. The sections on ligaments, the muscles, the viscera, and the nervous system are very important (874; 875; 877; 879). The loop of Henle in the nephritic unit of the kidney is named for him. He described these tubular loops as running perpendicular to the kidney surface and penetrating at a variable depth in the medulla. The descending portion of these loops had a small outer diameter (thin limb) as compared to that of the ascending portion located in the outer medulla (thick limb) (878).

Rudolf Albert von Kölliker (CH) confirmed Henle’s finding of smooth muscle in the endothelial wall of arteries (2001).

Henle and Karl von Pfeufer (DE) established the journal, Zeitschrift für Rationelle Medizin.

 

Friedrich Ludwig Hünefeld (DE) reported seeing brick red, sharp edged crystals of blood pigment in dried menstrual blood of humans and swine, which had been placed between glass plates in a desiccator (937). Note: The discovery of hemoglobin

 

William Bowman (GB) published his paper on the minute structure and functions of the striated, voluntary muscle. He coined the term sarcolemma (225; 226).

 

Louis-Michel-Francois Doyère (FR), Wilhelm Friedrich Kühne (DE), Charles Marie Benjamin Rouget (FR), and Louis-Antoine Ranvier (FR) demonstrated that motor nerves end on muscle fiber at a specialized structure, the plaque terminale, or endplate. There, the nerve loses its myelin sheath and branches on top of a cytoplasmic eminence of the muscle fiber particularly rich in nuclei (551; 1043; 1546; 1630; 1631).

 

Carlo Mattecci (IT) had demonstrated the electrical negativity of a cross section of muscle and proceeded to show the electrical oscillations in a tetanized muscle (1217).

 

Benedikt Stilling (DE) was the first to investigate vasomotor nerves (1803).

 

Frederick William Hope (GB) coined myiasis to refer to diseases of humans originating specifically with dipterous larvae (e.g., botfly, blowfly, fleshfly, and screwfly), as opposed to those caused by insect larvae in general, scholechiasis, which was coined by William Kirby (GB) and William Spence (GB) (914; 1009).

William S. Baer (US), an orthopedic surgeon at Johns Hopkins Medical School during the late 1920s, used maggot therapy to treat a series of patients with osteomyelitis, an infection of bone or bone marrow. The idea was based on an experience in World War I in which two soldiers presented to him with broken femurs after having lain on the ground for seven days without food and water. Dr. Baer could not figure out why neither man had a fever or signs of sepsis. He observed: "On removing the clothing from the wounded part, much was my surprise to see the wound filled with thousands and thousands of maggots, apparently those of the blow fly. The sight was very disgusting, and measures were taken hurriedly to wash out these abominable looking creatures." However, he then saw that the wounds were filled with “beautiful pink granulation tissue” and were healing well (64).

Throughout recorded history maggots have been used therapeutically to clean out necrotic wounds, an application known as maggot therapy. Maggot therapy – also known as maggot debridement therapy (MDT), larval therapy, larva therapy, or larvae therapy – is the intentional introduction by a health care practitioner of live, disinfected green bottle fly maggots (larvae) into the non-healing skin and soft tissue wound(s) of a human or other animal for the purpose of selectively cleaning out only the necrotic (dead) tissue within a wound in order to promote wound healing. Although maggot therapy has been used in the US for the past 80 years, the FDA approved it as a “medical device” only in 2004 (along with leeches in the same year). Maggots are approved for treating neuropathic (diabetic) foot ulcers, pressure ulcers, venous stasis ulcers, and traumatic and post-surgical wounds that are unresponsive to conventional therapies (1635). The American Medical Association and Centers for Medicare and Medicaid Services recently clarified the reimbursement guidelines to the wound care community for medicinal maggots, and this therapy may soon be covered by insurance (17).

 

Jules-Gabriel-François Baillarger (FR), while engaged in clinicopathological correlations with simple methodology, divided the cerebral cortex into six layers of alternate white and grey lamina. He was able to satisfy himself that the white lines seen by Francisco Gennari in the occipital area could be traced in all parts of the cortex, although they were far less conspicuous anteriorly than posteriorly. This continuation of Gennari's line has therefore come to be known as the "external line or white stripe of Baillarger". He discussed the connections between gray matter of the cerebral cortex and the internal white matter. He also demonstrated that the surface of the human brain in comparison to its own volume is less than that in smaller animals and that as a compensatory measure larger brains undergo greater fissuration than smaller ones – in short that the difference in external form of lissencephalic and gyrencephalic brains is explicable on the basis of the geometric law of volumes, that the volume increases as the cube of the diameter while the surface increases as the square (68).

Robert Remak (PL-DE) used microscopic observations to confirm Baillarger’s 6-layered cortex of the brain. He was the first to illustrate these layers (1592).

 

Pierre Adolphe Piorry (FR) and Sébastien Didier L’Héritier (FR) coined the term uraemia (uremia) to describe the condition in renal failure patients characterized by retention of products of urine in the blood (181; 1468).

Friedrich Theodor Frerichs (DE) described the clinical uremic syndrome and accepted a toxic mechanism as its etiology (709).

Joseph Picard (FR) developed a sensitive method to detect blood in urea. He was able to detect a 40% fall in urea concentration occurring between renal artery and vein (1467). This work along with Frerichs’ made popular the concept and the term uremia.

Victor-Timothée Feltz (FR) and Eugene Ritter (FR) declared that retained potassium is the uremic poison (647).

 

Moritz Heinrich Romberg (DE) wrote the first systematic treatise in neurology and a milestone in the development of clinical neurology. It included a classic on achondroplasia (congenital rickets). He and Peter Frank were pioneers in the study of the spinal cord. He described the classic, Romberg sign (swaying of the body with the feet close together and the eyes closed: a sign of locomotor ataxia) and stated that no ataxic can stand still with eyes shut. Patients with the tabes dorsalis of syphilis typically sway or lose their balance. He described Romberg's sign as: "The gait begins to be insecure... he puts down his feet with greater force...The individual keeps his eyes on his feet to prevent his movements from becoming still more unsteady. If he is ordered to close his eyes while in the erect posture, he at once commences to totter and swing from side to side; the insecurity of his gait also exhibits itself more in the dark." (1623; 1624)

 

Jonathan Mason Warren (US), about 1840, was one of the first surgeons to use free skin grafts. Small, entirely detached pieces of skin were taken from the arm or thigh to fill in gaps in a wound (2095).

 

Washington Joseph Duffee (US), in 1840, performed a primary amputation at the hip joint in a case of coxalgia. The patient experienced a full recovery (1270).

Edward Shipper (US) performed a successful amputation at the hip joint during the American Civil War (1381).

 

Richard Owen (GB), beginning in 1840, produced works of the highest importance in paleontology (1385; 1387; 1390; 1393; 1394).

 

John Phillips (GB), a nephew of William Smith (GB), proposed the geologic eras: Paleozoic (ancient life), Mesozoic (middle life), and Kainozoic which became (Cenozoic) (recent life) (1464). See, Smith in 1799.

 

1841

Rudolf Albert von Kölliker (CH) traced the histogenesis of the spermatozoa in invertebrates and proved that they are differentiated tissue cells and not parasites as thought by some. He also concluded that the physical basis of inheritance must be the chromosomes (1998).

Rudolf Albert von Kölliker (CH) demonstrated that spermatozoa of higher organisms are cellular products of the organism. He also extended this finding to the ovum, from which the organism is derived by cell division (2000).

 

Robert Remak (PL-DE) provided experimental evidence that only the division of pre-existing cells produces body cells of animals. He was probably the first to describe division of the cell nucleus and to observe the multiplication of vertebrate cells outside the body (1590; 1591; 1599; 1601).

 

Franz Joseph Andreas Nicolas Unger (AT) concluded that the growth of all parts of the plant is normally driven by meristematic cell division (1893; 1894).

 

The Magendie Commission (FR) reported, based on experiments using dogs, that no extract of bone could replace meat in the diet. Gelatin, albumen, and fibrin, taken separately, nourish animals for a very limited period. The seasoning of foods did not improve their nutritional value. Normal muscular flesh with its attendant gelatin, albumen, fibrin, fats, and salts supply sufficient nutrients for prolonged survival (later proved incorrect). Raw bones supply sufficient nutrients for prolonged survival but must be eaten in large quantities. Harsh treatment of meat and bone with high temperatures or acids substantially reduces their nutritive value. Gluten of wheat (Triticum spp.) or maize (Oryza sativa) supported prolonged survival (later proved incorrect). Fats alone cannot sustain life over a prolonged period (1185). (60)

 

Alexander Ure (GB) performed the first human metabolic study when he administered benzoic acid to himself and to volunteers. He succeeded in isolating large quantities of hippuric acid from their urine. Ure thus must be given the credit of being the first one to discover a biotransformation of a foreign compound. He proposed the use of benzoic acid for the treatment of gout (1897).

Friedrich Wöhler (DE) and Friedrich Theodor Frerichs (DE) confirmed this work using dogs (2158; 2159).

 

Gabriel Gustav Valentin (DE-CH) made the first observation of a trypanosome in the blood. The host animal was a trout (1899).

 

Friedrich Gustav Jacob Henle (DE), F. Berger (FR), Alfred Tulk (GB), and Carl Gustav Theodor Simon (DE) independently discovered Demodex folliculorum, the mite of hair follicles (108; 876; 1745; 1889).

 

Johannes Petrus Müller (DE) and Friedrich Gustav Jacob Henle (DE) made a thorough study of the cyclostomata, which they placed among the fishes. This work established the outline of elasmobranch taxonomy for the remainder of the 19th century (1315).

 

Martin Heinrich Rathke (DE) was the first to describe the lancet fish (Amphioxus lanceolatus). It had been considered to be the larvae of a mollusk (1568).

Johannes Petrus Müller (DE), Anders Adolf Retzius (SE), and Gustaf Magnus Retzius (SE) carefully studied and described Branchiostoma lanceolatum (Amphioxus lanceolatus, i.e., the lancelet) (1309; 1314; 1603).

 

Ferdinand Karl Franz von Hebra (AT) permanently shed the term “lepra” from the description of psoriasis, which ultimately separated the two diseases from one another once and for all. ref

 

Johann Friedrich Dieffenbach (DE) reported on 140 cases of tenotomy for treatment of clubfoot (528).

 

Valentine Mott (US), in 1841, removed a large fibrous growth from the nostril by dividing the nasal and maxillary bones (1280).

 

Richard Owen (GB), in his report to the Eleventh Meeting of the British Association for the Advancement of Science, coined the word dinosaur. "The combination of such characters, some, as the sacral ones, altogether peculiar among reptiles, others borrowed, as it were, from groups now distant from each other, and all manifested by creatures far surpassing in size the largest of existing reptiles, will, it is presumed, be deemed sufficient ground for establishing a distinct tribe or sub-order of Saurian reptiles, for which I would propose the name Dinosauria." He put together two Greek words: deinos meaning terrible and sauros meaning lizard. The result has been a favorite of paleontologists and children ever since. The original dinosaurs of this new group were Megalosaurus, Iguanodon and Hylaeosaurus (1386; 1866). See, Gideon Algernon Mantell, 1822 and William Buckland, 1824.

Although Richard Owen (GB) was an outstanding anatomist and museum curator he was not above pirating the work of others; such as that of Gideon Algernon Mantell (316).

 

Roderick Impey Murchison (GB) defined the Permian Period in geological history; named after the strata of the Perm region in Russia (1318).

 

1842-1844

Scotland experiences an epidemic of relapsing fever.

 

1842

Julius Robert Mayer (DE) was a brilliant theorist who presented the mechanical theory of heat. James Prescott Joule (GB) later received credit for this concept because of his many elegant experiments (982). Julius Robert Mayer also presented a logical argument for the conservation of energy in his law of the equivalence of heat and work. Hermann Ludwig Ferdinand von Helmholtz (DE) later received credit for this idea because of his experiments in which he proved mathematically that all forms of energy, such as heat, light, electricity, and chemical phenomena, can be transformed from one form to another but are indestructible as well as impossible of creation. He said that this conservation of energy is a fundamental principle of physics (1984). Mayer included living phenomena in the realm of energy conservation.

Julius Robert Mayer (DE) suggested that solar energy is the ultimate source of all energy on the earth, both living and non-living. He proposed that solar energy is derived from the slow contraction of the sun or from the fall of meteors into the sun. In either case kinetic energy was being converted into radiant energy. Hermann Ludwig Ferdinand von Helmholtz (DE) and William Thomson (Lord Kelvin) (GB) received credit for this idea (1221-1223; 2044).

Karl Friedrich Mohr (DE) was the first to record the concept of the conservation of energy (1259).

 

Johann Justus von Liebig (DE) noted that, "The blood corpuscles contain an iron compound. From the invariable presence of iron in red blood it must be concluded that it is essential for animal life, and… there can be no doubt that they (corpuscles) assume a role in the respiratory process." He discussed the tendency of some iron compounds to take up oxygen, and for the products to be reduced through the loss of oxygen… He called attention to the fact that in Prussian blue (ferrocyanide and ferricyanide) iron is present in combination "with all the organic constituents of the animal body: hydrogen and oxygen (water), carbon and nitrogen (cyanogen)" and concluded that… "blood corpuscles of the arterial blood contain iron compound that is saturated with oxygen, and in which the living blood loses its oxygen during the passage through the capillary vessels; the same occurs when blood is taken from the body and begins to decompose (begins to putrefy); the oxygen-rich compound is transformed by the loss of oxygen (reduction) into a less-oxygenated compound. One of the resultant products of oxidation is carbonic acid." He helped establish the view that body heat and vital activity arise out of energy derived from the oxidation of foodstuffs within the body and declared fats and carbohydrates—rather than carbon and hydrogen—the fuels of the body. He introduced the concept of stoffwechsel (metabolism) (2032; 2033). This book is regarded as the first formal treatise on organic chemistry as applied to physiology and to pathology.

 

Eilhard Mitscherlich (DE) used a parchment filter to divide a sugar solution into two compartments, one in contact with yeasts the other deprived of contact with yeast cells. Fermentation only took place where yeasts were in contact with sugar. He also found that yeast extract could convert cane sugar into a levorotatory sugar (1255).

Augustin Pierre Dubrunfaut (FR) then showed the levorotatory sugar to be a mixture of glucose and fructose (560).

 

Henri Milne-Edwards (FR) and Pierre-Joseph van Bénéden (BE) first observed ascidian tadpoles. Because they had reared them from eggs, both workers recognized that they were larval ascidians (1251; 1901). Note: Ascidiacea, commonly known as the ascidians, tunicates, and sea squirts.

 

John Edwards Holbrook (US), with the publication of North American Herpetology, became the leading American zoologist (908).

 

John Gill (GB), while working in the Madura region of India, in 1842, was the first to describe mycetoma, which later became known as Madura foot or maduromycosis (403; 748).

Alexandre Joseph Emilé Brumpt (FR) showed that several different fungi could cause the same clinical picture. He originated the Genus Madurella at this time (314).

Albert J. Chalmers (GB) and John B. Christopherson (GB) coined the term maduromycoses to refer to mycetomas of fungal etiology (358).

 

David Gruby (HU-FR) described ectothrix (Trichophyton ectothrix) invasion of the beard and scalp causing the disease Sycosis barbae (Barber's itch) (808). This organism would later be named Trichophyton mentogrophytes.

 

David Gruby (HU-FR) gave the first accurate description of Microsporon audouinii, the fungus of Willan's porrigo decalvans, tinea tonsurans, and Gruby's disease (809).

 

David Gruby (HU-FR) described endothrix hair invasion by Herpes (Trichophyton) tonsurans (810). This is called Tinea capitis (Ringworm of the scalp).

 

Hermann Ludwig Ferdinand von Helmholtz (DE) discovered that the nerve fibers originate in the ganglion cells. For the first time the connection between nerve fibers and nerve cells was established (1983). This represents the origin of the histological basis of nervous physiology and pathology.

Rudolph Wagner (DE) demonstrated the link between the ganglion cells of the brain and the peripheral nerve fibers (2069).

 

Charles Chossat (FR) studied the effect of diet on bone formation in pigeons. He found that birds on a wheat (Triticum spp.) only diet did not produce normal bones. If calcium carbonate was added to the wheat diet their bones were normal (374; 375).

 

Henry Bence Jones (GB) asserted that sodium chloride is indispensable in the human diet (978).

Jean Baptiste Joseph Dieudonné Boussingault (FR) used oxen to prove experimentally what man had known empirically for centuries, that is, that animals require sodium chloride in their diet (216-218).

 

Friedrich Heinrich Bidder (LV-DE) and Alfred Wilhelm Volkmann (DE) showed the sympathetic nervous system to consist largely of small, medullated fibers originating from the sympathetic and spinal ganglia (151).

 

William Bowman (GB) published his theory of urinary secretion and described the capsule which surrounds the “bare or naked system of capillaries,” called the Malpighian body (glomerulus), and is continuous with the uriniferous tubule (227). This capsule now bears his name as Bowman’s capsule.

Karl Friedrich Wilhelm Ludwig (DE) presented his physical theory of urine secretion by which a significant amount of liquid is removed from the blood by pressure in the glomerulus then partially recaptured by endo-osmotic flow from the urinary canals into the surrounding blood vessels. “The glomerular filtrate is driven…into the urinary vessels and thereby comes in contact with the blood which is flowing in the narrow vessels…and…has originated from the glomerulus [and] would draw water from the urinary canals into the blood vessels whereby the urine would become concentrated” (1151; 1152).

 

Alfred Francois Donné (FR) was the first to describe blood platelets or "globulins" (540). At the time he thought that globulins originated from lymph (les globulins du chyle) and in the late 1800s scientists described platelets variously as being precursors or disintegration products of erythrocytes, leukocytes, or fibrin and even as bacteria.

Maximillian Johann Sigismund Schultze (DE) was the first to describe the four different types of blood leukocytes corresponding to what we now recognize as the lymphocyte, the monocyte, the eosinophil, and the neutrophil. He also provided a description of the blood platelet (1708).

Carl Heitzmann (HU-AT-US) was one of the first to describe blood platelets as hematoblasts, thinking them to be precursors of erythrocytes (863).

Georges Hayem (FR) reported the first accurate platelet counts. The platelet numbers he reported do not differ significantly from those reported as normal today (645; 854; 855).

Giulio Cesare Bizzozero (IT) was the first to describe platelets as distinct blood elements referring to them as petites plaques. Because he used living animals for his studies, he was able to indicate the relationship of platelets to thrombosis and he described the changes platelets underwent when activated as "viscous metamorphosis." He said that in a blood clot, there is first the platelet thrombus followed by the more massive fibrin thrombus (179; 180).

Carl Joseph Eberth (DE) and Curt Schimmelbuch (DE) induced thrombus formation in mesenteric vessels of animals and regarded platelet agglutination as the initial phase of thrombosis (607).

James Homer Wright (US) described megakaryocytes as the cell of origin of platelets—he called them “cell plates” (2170).

William Waddell Duke (US) proposed that the bleeding time is closely correlated to platelet number and described a method for determining the bleeding time and coagulation time (576; 577). See, Milian, 1901.

James Homer Wright (US) and George Richards Minot (US) found that platelet viscous metamorphosis may occur before fibrin formation (2171).

Karl Albert Ludwig Aschoff (DE) classified the etiopathogenic components of thrombosis into three groups: parietal factors, hemostasis, and blood dyscrasia disorders (50).

 

Theodor Ludwig Wilhelm Bischoff (DE) provided the most comprehensive research into the history of the development of the ovum in Mammalia, which had been done so far. He specifically studied ovum development in man, dogs, guinea-pigs, and roe-deer (170-174).

 

William E. Clark (US), in January 1842, used ether to anesthetize a patient whose tooth was then extracted by doctor Elijah Pope (US) (1165). This is the first recorded use of ether as an anesthetic.

Crawford Williamson Long (US) was the first physician to use ether as an anesthetic in surgery. "Since '42, I have performed one or more surgical operations annually, in patients in a state of etherization…. From one [patient] I removed three tumours the same day…. I amputated two fingers of a negro boy." (1133)

Charles Thomas Jackson (US) is one of the people who claimed to have been the first to use ether as an anesthetic. Jackson's claims for priority were substantially as follows: He had already experimented on the anesthetic properties of chloroform and of nitrous-oxide gas, and previous to the winter of 1841-'2, having received some perfectly pure sulfuric ether, he tried its effects upon himself, administering it with a mixture of atmospheric air, and inhaled it to such an extent as to lose all consciousness, without suffering any of the dangerous or disagreeable consequences that had hitherto attended the inhalation of impure sulfuric ether unmingled with atmospheric air. In 1852 a memorial was presented to congress, signed by 143 physicians of Boston and its vicinity, ascribing the discovery exclusively to Dr. Jackson. Jackson never published.

 

Charles Oscar Waters (US) described a form of chorea (magrums), including an accurate description of its progression, and the strong heredity of the disease (582). This later became known as Huntington's disease.

Johan Christian Lund (NO) was the first to describe setesdalsrykkja or rykkja, i.e., “jerking disease” (Huntington’s disease) (1156; 1157).

George Huntington (US) wrote his classic paper in which he diagnosed hereditary chorea (now called Huntington’s disease). He noted the familial nature of the disorder and provided a graphic description of it (939).

William Bateson (GB) used the pedigrees of affected families to establish that Huntington's disease did have an autosomal dominant inheritance pattern (87). This was the first human genetic disease inherited in the autosomal dominant mode to be uncovered.

Alois Alzheimer (DE) recognized cortical loss in Huntington’s but emphasized that it was striatal damage that caused the motor disturbances (29).

James F. Gusella (US), Nancy S. Wexler (VZ), P. Michael Conneally (US), Susan L. Naylor (US), Mary Anne Anderson (US), Rudolph E. Tanzi (US), Paul C. Watkins (US), Kathleen Ottina (US), Margaret R. Wallace (US), Alan Y. Sakaguchi (US), Anne B. Young (VZ), Ira Shoulson (VZ), Ernesto Bonilla (VZ), and Joseph B. Martin (US) of The US–Venezuela Huntington's Disease Collaborative Research Project discovered the approximate location of a causal gene for Huntington's disease (817; 818).

Marcy E. MacDonald (US), Christine M. Ambrose (US), Mabel P. Duyao (US), Richard H. Myers (US), Carol Lin (US), Lakshmi Srinidhi (US), Glenn Barnes (US), Sherryl A. Taylor (US), Marianne James (US), Nicolet Groot (US), Heather MacFarlane (US), Barbara Jenkins (US), Mary Anne Anderson (US), Nancy S. Wexler (US), James F. Gusella (US), Gillian P. Bates (GB), Sarah Baxendale (GB), Holger Hummerich (GB), Susan Kirby (GB), Mike North (GB), Sandra Youngman (GB), Richard Mott (GB), Gunther Zehetner (GB), Zdenek Sedlacek (GB), Annemarie Poustka (GB), Anna-Maria Frischauf (GB), Hans Lehrach (GB), Alan J. Buckler (US), Deanna Church (US), Lynn Doucette-Stamm (US), Michael C. O'Donovan (US), Laura Riba-Ramirez (US), Manish Shah (US), Vincent P. Stanton (US), Scott A. Strobel (US), Karen M. Draths (US), Jennifer L. Wales (US), Peter Dervan (US), David E. Housman (US), Michael Altherr (US), Rita Shiang (US), Leslie Thompson (US), Thomas Fielder (US), John J. Wasmuth (US), Danilo Tagle (US), John Valdes (US), Lawrence Elmer (US), Marc Allard (US), Lucio Castilla (US), Manju Swaroop (US), Kris Blanchard (US), Francis S. Collins (US), Russell Snell (GB), Tracey Holloway (GB), Kathleen Gillespie (GB), Nicole Datson (GB), Duncan Shaw (GB) and Peter S. Harper (GB) discovered the precise causal gene for Huntington's disease at 4p16.3, making this the first autosomal disease locus found using genetic linkage analysis (1171).

 

A Mr. Walker (GB) reported on a case in which the patient exhibited sympathetic ophthalmia. The right eye sustained such a severe laceration that sight was lost in that eye. Six months later the left eye exhibited inflammation and sight was slowly lost in that eye also. Some several months after inflammation subsided in the left eye an operation to restore vision in it was successful (1).Note: This was not the first clinical case of sympathetic ophthalmia, however it was one of the first cases in which sight was successfully restored to the sympathetic eye.

 

Domenico Antonio Rigoni-Stern (IT) examined the death records of the city of Verona for the years of 1760 to 1839. He found that cancer of the cervix of the uterus was rare in nuns, but the nuns were afflicted with a veritable epidemic of breast cancer. Housewives had breast cancer far less frequently than nuns, but cancer of the cervix was widespread among them (1611; 1612).

Dimitrios Trichopoulos (GR-US), Brian MacMahon (US), and Philip Cole (US) compared the reproductive experience of women with breast cancer with that of women of comparable ages who did not have it. They found that a woman who had a live born infant at an early age was afforded considerable protection against breast cancer when compared to women who had not delivered such an infant while they were young. They also showed that a woman who had never given birth to a full term, live born infant was twice as likely to develop breast cancer as a woman who had borne one at any age (1879).

 

Norman Chevers (GB) described aortic subvalvular stenosis (365).

 

James Syme (GB) performed his first innovative ankle disarticulation (1659).

 

Pierre Michel Toussant de Serres (FR) wrote the first scientific study of animal migrations (509).

 

Johannes Japetus Smith Steenstrup (DK) discovered the possibility of using the subfossils (not yet truly fossilized) of the post glacial as a means of interpreting climate changes and correlated vegetation change, which he called succession in the recent past (447; 1795).

 

Archiv für Physiologische Heilkunde was founded.

 

Zeitschrift für Rationelle Medicin was founded.

 

1843

"The plant cell, like the animal cell, is a type of laboratory of cellular tissues that organize themselves and develop within its innermost substance; its imperforate walls, to judge from our strongest magnifying instruments, have the property of drawing out by aspiration from the ambient liquid the elements necessary for its elaboration. Thus, they have the property of acting as a sorter, of admitting certain substances and preventing the passage of others, and consequently of separating the elements of certain combinations in order to admit only a portion of them.

Since disease originates in the elementary cell, the organization and microscopic functions of which reproduce the general organization exactly and in all its relationships, nothing is more suited to simplifying the work of classification and of systematic division than to take the elementary cell as the basis of division." Francois-Vincent Raspail (FR) (1552)

With thinking such as this Raspail helped point the way to the cell theory and to cellular pathology.

 

Adolph Wilhelm Hermann Kolbe (DE) carried out the second "total synthesis" of an organic compound (acetic acid) from truly inorganic precursors. Carbon disulfide was the starting material (1031; 1032).

 

Jean Baptiste André Dumas (FR) published the empirical formula for glucose (579).

 

Joseph Redtenbacher (CZ) distilled glycerol with a small addition of phosphorus pentoxide and obtained acrolein (acrylic aldehyde), which he identified with the highly irritating vapors formed when fats are heated to decomposition. This odor formation was subsequently employed as a qualitative test for fats (1578).

 

Johann Justus von Liebig (DE) surmised that organic acids might be the intermediate substances on the path of carbon from carbon dioxide (CO2) to carbohydrates. He proposed that various organic acids, such as oxalic, malic, tartaric and citric acids, were the intermediates in the stepwise reduction of CO2 to carbohydrates (1340; 1430; 2035).

 

Johann Justus von Liebig (DE) proposed that carbohydrates could serve as precursors to fats. He suggested this based on his observation that farmers fattened their cattle by feeding them grains. He noted that to make fat from sugar would simply require the removal of oxygen from sugar molecules (2034).

DeWitt Stetten, Jr. (US) and George E. Boxer (US) concluded from their experiments that in animals fed a high carbohydrate diet, fatty-acid formation is a more important pathway in glucose utilization than glycogenesis (1801).

 

Julius Vogel (DE) showed that cholesterol is present in atherosclerotic plaques (1943).

 

George Busk (GB), in 1843, was the first to describe a clinical case of fasciolopsiasis (giant intestinal fluke). He found 14 flukes in the duodenum of a sailor from eastern India during an autopsy. The discovery was not published.

George Budd (GB) clearly documented this fluke. It was later named Fasciolopsis buski by others and recognized as the trematode fluke causing fasciolopsiasis (321; 644).

Thomas Spencer Cobbold (GB) described this intestinal fluke in detail (386).

Claude Heman Barlow (US) described the life cycle of the human intestinal fluke, Fasciolopsis buski, he studied in the Shaohsing region of the Chekiang Province of China (77).

 

Angelo Dubini (IT), in 1838, observed a “new human intestinal worm” following the dissection of the corpse of a peasant woman who had “died of croupous pneumonia”. This hookworm is the agent causing ankylostomiasis, the hookworm disease. He named the hookworm, Ancylostoma duodenale (557; 558).

Theodor Maximillian Bilharz (DE) proposed a connection between hookworms (Ancylostoma) and Egyptian chlorosis (159).

Wilhelm Griesinger (DE) recognized Ancylostoma as the cause of Egyptian chlorosis (798; 799).

R.J. Lee (GB) was the first to describe cutaneous larva migrans (CLM) which he called "the creeping eruption" (1092). Today, it is one of the most common helminth (hookworm) infections (usually caused by Ancylostoma braziliense) acquired from subtropical and tropical regions of the world.

Camillo Bozzolo (IT) introduced thymol as a treatment for hookworm infestation (229).

Arthur Looss (DE), working in Egypt, was dropping cultures of hookworm larvae into the mouths of guinea pigs when he spilled some of the culture onto his hand. He noticed that it produced an itching and redness and wondered if infection would occur this way. He began examining his feces at intervals and, after a few weeks, found that he was passing hookworm eggs. He elucidated the entire life cycle of the parasitic hookworm nematode called Ancylostoma duodenale (1136-1139).

 

James Braid (GB) coined the terms hypnosis, hypnotism, hypnotize, and hypnotist (235).

 

Jean Louis Rodolphe Agassiz (CH-US) completed Recherches sur les Poissons Fossiles… describing the fossil fish of the world. This single monograph increased tenfold the formally described vertebrates known to science (20).

 

Pierre-Joseph van Bénéden (BE) founded the first marine biological laboratory. It was located at Ostend, Belgium.

 

1844

Johann Justus von Liebig (DE) wrote Bemerkungen uber das Verhaltniss der Thierchemie zur Thier -Physiologie, which united the fields of chemistry and physiology (2036).

 

Heinrich Gustav Magnus (DE) and Julius Bodo Unger (DE) isolated guanine from the guano of birds. This was forty years prior to its recognition as a nucleic acid constituent (1191; 1895; 1896).

 

Johann Florian Heller (AT) devised the "Ring Test" for albumin (864).

 

Johann Florian Heller (AT) devised the caustic "Potash Test" for sugar in urine (865).

 

Théophile Jules Pelouze (FR) and Amédée Gélis (FR) were the first to describe the synthesis of a natural neutral lipid, tributyrin, by reacting butyric acid with glycerine in the presence of concentrated sulfuric acid (1447).

 

Nicolas-Theodore Gobley (FR) described the isolation from egg yolk of a substance, which contained both nitrogen and phosphorus in addition to glycerol and fatty acids. He called it lecithin (Gk. lekithos, egg yolk) (752-754; 756).

Ernst Felix Immanuel Hoppe-Seyler (DE) rediscovered lecithin, a fat (phospholipid) containing nitrogen and phosphorus (917).

 

Carl Schmidt (DE) coined the term kohlenhydrate, i.e., carbohydrate to include sugars, starches, and other natural products whose precipitates contained carbon plus hydrogen and oxygen in the same ratio as in water (CH2O) (1686). Schmidt was the first to demonstrate the presence of sugar in the blood

 

Karl Friedrich Gaertner (DE) distinguished spores from seeds, and endosperm from cotyledons. He noted the distinction between the uniformity of the first hybrid generation and the diversity of later generations, and reported hybrid vigor (723-725).

 

Jean Baptiste Joseph Dieudonné Boussingault (FR) performed experiments, which showed that animals couldn’t live on beetroots or potatoes (Solanum tuberosum) alone without sustaining a weight loss. He concluded that animals could not use nitrogen from the atmosphere to supplement inadequate protein in their diet (213).

 

Jean Baptiste Joseph Dieudonné Boussingault (FR) and Jean-Francois Persoz (FR) offered experimental proof that animals can synthesize fat from carbohydrate (212; 1451).

 

Eduard Friedrich Wilhelm Pflüger (DE) recognized that hypoxia stimulates ventilation of the lungs (1463).

 

Max Josef Pettenkofer (DE) devised a qualitative test for bile salts (cholic acid) (1454).

 

Max Josef Pettenkofer (DE) discovered kreatinin (creatinine) in human urine then devised a qualitative test for it (1455).

Max Jaffe (DE) reported that creatinine in a solution of alkaline picrate produced an intense red color. He found the amount of precipitate directly proportional to the creatinine concentration, he also noted that the reaction was highly nonspecific and could be observed with many other organic compounds (974). See, Folin 1914.

 

Agostino Bassi (IT) suggested that parasites were the causes of various diseases (including plague, smallpox (red plague), syphilis, and cholera) and advocated methods of prevention. He became the founder of the doctrine of parasitic microbes (82).

 

Joseph Lister (GB), in 1844, made clinical use of Penicillium saying, "Should a suitable case present, I shall endeavor to employ Penicillium glaucum and observe if the growth of the organisms be inhibited in the human tissues." Such an opportunity did arise, and he reported using Penicillium to great effect on a patient injured in a road accident (936).

John Tyndall (GB), in 1875, described to the Royal Society in London how a species of Penicillium had made several bacteria burst open and die (1891).

Ernest Duchesne (FR) described in his dissertation the effectiveness of Penicillium glaucum in animals injected with normally fatal doses of pathogenic bacteria. In a subsequent article he emphasized the therapeutic value of Penicillium (567; 568).

André Gratia (BE) and Sara Dath (BE) mentioned that a Penicillium strain exerted a highly bacteriolytic activity against anthrax-causing bacteria (788).

Georges Papacostas (FR) and Jean Gaté (FR) used the term antibiotic and reported clinical applications of antibiotic substances (1412).

Alexander Fleming (GB) observed that some mold contaminants of his plates of Staphylococcus aureus exerted a specific antagonism toward the bacteria. He postulated that the mold Penicillium produced a potent antibacterial agent, naming the agent penicillin and observing that it killed gram-positive bacteria more effectively than gram-negative bacteria (682).

Cecil George Paine (GB) was the first person to obtain effective cures with the drug. In 1930, he administered crude penicillin topically to successfully treat patients with eye infections at Sheffield Hospital. In this crude form it could not successfully treat many infections. Note: Paine never published this work.

Ernst Boris Chain (DE-GB), Howard Walter Florey (AU-GB), Arthur Duncan Gardner (GB), Norman G. Heatley (GB), Margaret Augusta Jennings (GB), Jena Orr-Ewing (GB), A. Gordon Sanders (GB), Edward Penley Abraham (GB), and Charles M. Fletcher (GB) developed the cultural and chemical methodology to produce pure penicillin from Penicillium and found that it displayed potent in vivo antimicrobial activity against certain pathogens. This purified antibiotic was first used clinically on an Oxford, England policeman (Albert Alexander) suffering from staphylococcal pyemia (7; 357).

Anne Miller (US), in 1942, became the first American civilian patient to be successfully treated with penicillin. She was lying near death at New Haven Hospital in Connecticut, after miscarrying and developing an infection that led to blood poisoning.

Kenneth Bryan Raper (US), working at the Northern Regional Research Laboratory, isolated Penicillium chrysogenum strain NRRL 1951 from a moldy cantaloupe brought to him in 1943 by Mary Hunt, a Peoria, Illinois housewife. This strain could produce large quantities of penicillin in submerged culture and subsequently became the parent of most all strains used in the production of penicillin. Once larger quantities of the antibiotic were available it was used to treat war casualties starting in Tunisia and Sicily in 1943.

 

Robert Remak (PL-DE) was the first to describe the intrinsic nerve ganglia of the heart (Remak’s ganglia). This helped explain the relatively autonomous nature of the heart beat (1592).

Robert Remak (PL-DE) discovered the nerve ganglion located in the sinus venosus of the frog’s heart (1594).

Karl Friedrich Wilhelm Ludwig (DE) discovered the ganglion cells of the interauricular ganglion of the heart (Ludwig's ganglion) (1154).

Friedrich Heinrich Bidder (LV-DE) discovered two large masses of ganglion cells at the juncture of the auricles and ventricles of the heart (148).

 

Rudolf Albert von Kölliker (CH) demonstrated the relative independence of the sympathetic nervous system (1999).

 

Claude Bernard (FR), in one of the earliest cardiac catheterizations, inserted a mercury thermometer into the carotid artery of a horse and advanced it through the aortic valve into the left ventricle to measure blood temperature. He adapted this experiment over the next forty years for measuring intracardiac pressures in a variety of animals. It was Bernard who coined the phrase cardiac catheterization (1281).

 

James Young Simpson (GB), in 1844, introduced into obstetrical practice dilatation of the cervix uteri for diagnostic purposes (1752; 1758; 1760). He was the first to use chloroform in obstetrics and the first in Britain to use ether (1755). Simpson introduced the terms ovariotomy and coccydynia (1760).

 

Auguste Nélaton (FR) placed alcohol-soaked dressings on open wounds and reported a decreased infection rate (1331).

 

Theodor Ambrose Hubert Schwann (DE), using an artificial biliary fistula in a dog, established that bile from the liver and gall bladder is indispensable to digestion (1723).

 

Karl Moritz Gottsche (DE), Johann Bernhard Wilhelm Lindenberg (DE), and Christian Godfried Daniel Nees von Esenbeck (DE) brought together in one publication the descriptions of the world’s known hepatics (liverworts); some 1,600 species (777).

 

Hermann Schlegel (NL) was the first to use a third name—ternary nomenclature—added to the Linnean binomial when he used it to designate geographic variants among birds (1742).

 

Friedrich A. Puchelt (DE) gave the first clinical report describing astereogenesis or failure to identify the form of an object being held (1519).

 

Arthur L. Wigan (GB) wrote philosophically about each hemisphere of the brain housing its own distinct mind. Wigan wrote that the two minds ordinarily worked in a unified, highly adaptive manner, but also possessed the ability to function independently (2128).

Walter Edward Dandy (US) argued that no symptoms follow splitting of the corpus collosum (473).

Roger Wolcott Sperry (US), Ronald Myers (US), Joseph E. Bogen (US), Philip J. Vogel (US), and Michael S. Gazzaniga (US) reported on cases of cerebral commissurotomy (brain splitting) in cats, monkeys and epileptic patients in whom the corpus callosum — the bundle of axons fibers that connects the two brain hemispheres — had been severed to prevent seizures. Several tests revealed how the two brain hemispheres hold independent streams of conscious awareness, perceptions, thoughts, and memories and, importantly, that neuronal connections are formed and maintained with a high degree of precision (190; 191; 1320; 1778; 1781).

Roger Wolcott Sperry (US) reported that under controlled testing conditions, the left hemisphere showed that it is much more verbal than the right, as well as more analytic and more rational. The right hemisphere, in turn, came out being more holistic, emotional, impulsive, and artistic (1779; 1780).

Michael S. Gazzaniga (US) later wrote, "Results accumulated over a period of six years demonstrated that the cortical commissures were critical to the inter-hemispheric integration of perceptual and motor function. These studies also revealed that the mute right hemisphere was specialized for certain functions that deal with nonverbal processes, while, not surprisingly, the left hemisphere was dominant for language. For the first time in the history of brain science the specialized functions of each hemisphere could be positively demonstrated as a function of which hemisphere was asked to respond." (1106). This work forms the basis of human developmental neurobiology and psychobiology.

 

Ernst Maria Johann Karl von Feuchtersleben (AT), in 1844, introduced the term psychosis in its modern context during lectures on psychiatry (1971).

 

Robert Chambers (GB), first writing anonymously and then under the pseudonym Samuel Richard Bosanquet, introduced the idea of evolution to the wider public in a popular book. He argued for an ancient solar system begun in fire, then coalescing under gravity as it cooled and geological processes, violent at first, then gradually subsiding over eons. He reasoned that life arose by a natural and material process with simple forms gradually giving rise to more complex forms. Man was a product of this process (2; 200).

 

c. 1845

Rudolf Buchheim (EE), in Dorpot, Estonia, set up the world’s first laboratory devoted to the study of the actions of drugs (418).

 

1845

"What exact relation disease of the kidney bears to hypertrophy of the heart, we do not know even yet. But the two are too often coincident in the same subjects for them not to bear some, and that a very important, relation to each other." Peter Mere Latham (1073)

 

Alfred Francois Donné (FR) produced the first engravings from photomicrographs, in this case, daguerreotypes (542). He had shown these photomicrographs to the French Academy of Science in 1839.

 

Raffaelle Piria (IT) described salicin (salicyl alcohol glucoside) from willow bark (1469).

 

Raffaelle Piria (IT) showed that aspartic acid is converted to malic acid upon treatment with nitrous acid (1470).

Victor Dessaignes (FR) made aspartic acid from malic acid or fumaric acid (520).

 

John F.W. Herschel (GB) described the fluorescent properties of quinine sulfate ushering in the 'modern' period for observing fluorescence and realizing its nature (889).

George Gabriel Stokes (GB) described a vast collection of fluorescent substances, from quinine sulfate to Oporto wine. It was he who coined the word fluorescence to describe light emission induced during excitation (1804).

Johann Friedrich Wilhelm Adolf Baeyer (DE) synthesized the first fluorescent dye, fluorescein (65; 66).

 

Carl Schmidt (DE) discovered that the tunicates contain cellulose (1687). This was unexpected since cellulose was thought to be unique to plants.

 

Nicolas-Theodore Gobley (FR) described in egg yolk and brain the presence of a phosphorus-containing fraction, which gave by hydrolysis oleic acid, margaric acid, and phosphoglyceric acid (755; 757; 758). This represents the discovery of phospholipids.

Johannes Ludwig Wilhelm Thudichum (DE-GB) coined the term phosphatide to designate phosphorus-containing lipids (1849).

 

Julius Robert Mayer (DE) was the first to state that the fundamental physical function of photosynthesis is the conversion of light energy into chemical energy saying: "Nature has put itself the problem of how to catch in flight light streaming to the Earth and to store the most elusive of all powers in rigid form. The plants take in one form of power, light; and produce another power, chemical difference." (1222).

 

John Reid (GB) and Pierre-Joseph van Bénéden (BE) independently discovered entoproct larvae (1586; 1900). Note: Entoprocta (Kamptozoa) is an enigmatic, acoelomate, tentacle-bearing phylum with indirect development, either via a swimming- or a creeping-type larva and still debated phylogenetic position within Lophotrochozoa.

 

Johann Dzierzon (PL), a Polish apiarist, discovered the phenomenon of parthenogenesis in bees and designed the first successful movable-frame beehive (603).

 

Apollinaire Bouchardat (FR) and Claude M.S. Sandras (FR) detected a diastase in the pancreas but were unsure of their findings (204).

 

Ernst Heinrich Weber (DE) and Eduard Friedrich Wilhelm Weber (DE) shared the discovery of the inhibitory power of the vagus nerve upon the beating of the heart in frogs, fish, birds, cats, dogs, and rabbits (2098; 2101).

 

Robert Dundas Thomson (GB) was the first to show that after a meal rich in fat, the blood level of fat will rise (1846).

Robert Dundas Thomson (GB) and Friedrich Knapp (DE) are credited with devising a method for determining animal rations, using data from chemical analysis of foods and excreta (1847).

 

Miles Joseph Berkeley (GB) insisted that a fungus, Botrytis, (later names Phytophthora infestans [Mont.] De Bary) was responsible for the potato blight which attacked the crops of Great Britain and Ireland in 1845. He did not, however, prove this. Berkeley was a leader in taxonomic mycology in England where he named approximately six thousand new species of fungi (112; 114-117; 1765).

Heinrich Anton de Bary (DE) and Mikhail Stepanowitsch Woronin (DE) reported having inoculated spores of Phytophthora infestans (formerly Peronospora infestans) on healthy potato leaves and observed the penetration of the leaf and the subsequent growth of the mycelium that affected the tissue, the formation of conidia, and the appearance of the characteristic black spots of the potato blight. He also did similar experiments on potato stalks and tubers. He watched conidia in the soil and their infection of the tubers, observing that mycelium could survive the cold winter in the tubers. From all these studies, he concluded that organisms could not be generated spontaneously (489; 491; 495).

 

Bernhard Rudolf Konrad von Langenbeck (DE), in 1845, was the first to report a case of what would later be called human actinomycosis. It occurred in a patient with vertebral caries and was attributed to a fungus. James Adolf Israel (DE) later reported Langenbeck’s discovery (967).

Otto Bollinger (DE) was the first to recognize tumors (lumpy jaw of cattle) caused by Actinomyces bovis as a specific parasitic disease. At this time the organism had neither been named nor classified (197; 198).

Karl Otto Harz (DE), at the suggestion of Otto Bollinger (DE), studied parasitic material isolated from osteosarcomic tumors, lumpy jaw, in cattle and gave it the name Actinomyces bovis because of the ray-like structure of its growth in the tissues (848).

James Adolf Israel (DE) and Emil Ponfick (DE) discovered granules in human autopsy material and described actinomycosis in humans. Thoracic actinomycoses was described for the first time in the 1882 reference (967-969; 1489; 1490).

James Adolf Israel (DE) described human cases of actinomycosis very similar to lumpy jaw in cattle (967).

Max Wolff (DE) and James Adolf Israel (DE) delineated the anaerobic nature of actinomyces (Actinomyces israelii), obtained axenic cultures of Actinomyces israelii from man, and proved their pathogenicity by animal inoculations (2166).

Selman Abraham Waksman (RU-US) showed that actinomyces are gram-positive bacteria (2071).

 

John Leconte (US) described the nervous system of the alligator (1091).

 

Johann Franz Simon (DE) performed clinical trials for the treatment of chlorosis (green sickness, a peculiar anemia most likely to affect young girls about the time of puberty) by iron administration. He monitored the patient’s progress and the success of the treatment by analyzing the blood for hematin (iron protoporphyrin) content (1747). Chlorosis is very likely the equivalent of what is today called iron deficiency anemia.

Knud Helge Faber (DK) gave a clinical description of achylia gastrica (atrophic gastritis) which is very suggestive of iron deficiency anemia (635).

 

Karl Theodor Ernst von Siebold (DE) and Hermann Friedrich Stannius (DE) were the first to introduce the taxa Arthropoda, Rhizopoda, and define the taxon Protozoa in their present sense. They made it quite clear that protozoa are single cell life forms and established Protozoa as the basic phylum of the animal kingdom. They were the first to study cilia and showed that they could be used for locomotion (2061; 2062).

 

John Goodsir (GB) established that cells are the active structures involved in glandular secretion, and observed that bone-forming properties reside with certain corpuscles (cells) found within osseous tissue. This represents the foundation of the study of osteogenesis, as distinct from descriptive osteology. He determined that the cell nucleus must be regarded as the central organelle of the cell (772; 773). Rudolph Ludwig Karl Virchow (DE) dedicated his 1859 book, Cellularpathologie, to Goodsir because of his advances of knowledge at the cellular level.

 

Hermann Lebert (DE) wrote Physiologie Pathologique, one of the earliest and most important atlases of pathological histology. This work played an important role in introducing the idea of cellular pathology (1087).

 

Robert Bentley Todd (IE-GB), by inductive reasoning and not experiment, was the first to call attention to the functions of the posterior columns of the spinal cord. "The posterior columns of the spinal cord may be in part commissural between the several segments of the cord, serving to unite them and harmonize them in their various actions, and in part subservient to the function of the cerebellum in regulating and coordinating the movements necessary for perfect locomotion." (1862)

Charles-Édouard Brown-Séquard (FR) proved by experiment the truth of Todd’s view. See, Brown-Séquard, 1855.

 

Robert Bentley Todd (IE-GB) discussed the role of the cerebral cortex in mentation (the thinking process), corpus striatum in movement, and midbrain in emotion (1862). Verify

 

Karl Wilhelm Ludwig Bruch (DE) wrote his dissertation on rigor mortis (311).

 

1846-1851

Sweden experiences an epidemic of cerebrospinal meningitis.

 

1846

"We must assume also that the organization of this substance is the process that inaugurates the formation of new cells. It therefore seems justifiable for me to propose a name that refers to its physiological function: I propose the word protoplasma." Hugo von Mohl (2051).

"It has long been an important problem in medical science to devise some method of mitigating the pain of surgical operations. An efficient agent for this purpose has at length been discovered. A patient has been rendered completely insensible during an amputation of the thigh, regaining consciousness after a short interval. Other severe operations have been performed without the knowledge of the patients." Henry Jacob Bigelow (156)

 

Auguste Laurent (FR) developed at length the difference between an atom and a molecule (1078).

 

Johann Justus von Liebig (DE) produced crystals of the amino acid tyrosine (Gk. tyros, cheese) by treating casein with alkali (2037; 2038).

Friedrich Bopp (DE), a colleague of von Liebig’s, studied tyrosine much more carefully (199).

 

Claude Bernard (FR) isolated lipase from pancreatic juice (128). Note: The publication lagged the discovery by ten years.

 

Augustin Pierre Dubrunfaut (FR) discovered maltose as a major product of the breakdown of starch by diastase (amylase) and described it as a disaccharide (559). He determined that acid would convert cane sugar into a mixture of sugars called invert sugar. He later showed that the invert sugar mixture included dextrose (glucose) and levulose (fructose) (560).

 

Hugo von Mohl (DE) studied the contents of plant cells and used the word protoplasm (coined by Purkinje), to designate the physical basis of life (2051). See, Purkinje, 1839.

 

Eben Norton Horsford (US) developed a method for the quantitative estimation of plant fiber (923).

 

Jean Baptiste Joseph Dieudonné Boussingault (FR) performed experiments, which allowed him to calculate the minimum amount of dietary calcium phosphate necessary for normal skeletal development in the pig (215).

 

Max Josef Pettenkofer (DE) produced one of the earliest pieces of evidence that food has a determining influence on the composition of urine (1456). Verify

 

Golding Bird (GB) weighed and determined the specific gravity of 24-hour urine collections. From these values and a formula, he calculated the quantity of solids in 1000 grains of urine. He also used nitric acid to test for bile in urine (a green color), and a simple polariscope or alkaline copper reagent for glycosuria (166).

 

Heinrich Freidrich Link (DE) presented a classification of inflorescence types and determined that filaments of lichens and fungi consist of cells (2183).

 

Johannes Petrus Müller (DE) described phoronids (horseshoe worms) as larvae before they were known as adults. He found large numbers of actinotrochs at Heligoland, to which he assigned genus and species names: Actinotrocha branchiata (1310-1312).

Thomas Strethill Wright (GB) discovered adult phoronids (2172).

Alexander Onufrievich Kowalevsky (RU) clarified the relationship between larvae and adults in the phoronids (1037).

 

The first Hereford Herd Book was published and later adopted by the 'Hereford Herd Book Society', founded in 1878.

 

John James Audubon (US) and John Bachman (US) authored Viviparous Quadrupeds of North America, which, among other things, gave the first substantial report on the mammals of North America (56).

 

Karl Friedrich Wilhelm Ludwig (DE) adapted the kymograph (cymograph) to record physiological phenomena. Ludwig made his first tracing on December 12th, 1846 (1153). Physiologists first used kymographs for recording blood pressure. The detailed study of blood pressure made possible in this way showed that blood circulation could be explained in terms of ordinary mechanical forces. This machine represents one of the first graphic methods employed in experimental physiology. Ludwig by simultaneously recording the pulse wave and respiratory pattern became the first to described sinus arrhythmia in 1847. Note: Thomas Young (GB) invented the kymograph (wave writer) in 1807 (2176). His invention was suggested by the work of Jean Marie Constant Duhamel (FR) who attached a pencil to a tuning fork in such a way that it reproduced the vibrations of the tuning fork as a wavy line. This was also the precursor of the phonograph.

 

Peter Ludwig Panum (DK) is responsible for our modern understanding of the epidemiology of measles. Having been sent by the Danish government to study an outbreak of the disease on the remote Faeroe Islands in the North Atlantic he demonstrated that measles is contagious, discerned both the length of the incubation period, and the establishment of a lifelong immunity in those who recovered from this infection.

"If among 6,000 cases of which I myself observed and treated about 1,000, not one was found in which it would be justifiable, on any grounds whatever, to suppose a miasmatic origin of measles, because it was absolutely clear that the disease was transmitted from man to man and from village to village by contagion, whether the latter was received by immediate contact with a patient, or was conveyed to the infected person by clothes, or the like, it is certainly reasonable at least to entertain a considerable degree of doubt as to the miasmatic nature of the disease…. It is beyond doubt that the surest means of hindering the spread of the disease, is to maintain quarantine." (1411) Note: This is a republishing of Panum’s classic paper in 1846

Francesco Cenci (IT), in 1901, was the first to use convalescent serum to induce passive immunization in individuals exposed to measles (rubeola) (355).

Charles Nicolle (FR) and Ernest Conseil (FR) confirmed the effectiveness of the passive immunization procedure for measles (rubeola) (1341).

 

Carl Ferdinand Eickstedt (DE) discovered that Tinea versicolor is the cause of Pityriasis versicolor, a superficial dermatomycosis in man (622).

Theodorus Slyter (DE) made the same discovery (1763).

Henri Baillon (FR) named the causative agent Malassezia furfu (70).

 

Ferdinand Karl Franz von Hebra (AT), in 1846, under the name of seborrhea congestiva described disc-shaped patches and introduced the butterfly simile for the malar rash.

Pierre-Louis Alphée Cazenave (FR) gave the first good clinical description of what he named lupus erythemateaux (lupus erythematosus) (350-352). His teacher Laurent-Théodore Biett (CH-FR) had identified a special variety within the erythema genus, the erythema centrifugum or centrifugal erythema; and the variety of lupus that destroys on the surface (154). See, J. Darier 1916-1917. See, Hargraves 1948. Note: The name “lupus” (Latin, wolf) described the cutaneous lesions of lupus that could ulcerate and eat away at the patient, as would a carnivorous animal.

Moriz Kohn Kaposi (HU), in 1872, subdivided lupus into the discoid and systemic forms and introduced the concept of systemic disease with a potentially fatal outcome.

 William Osler (CA) also described lupus at a slightly later date, calling it erythema exudativum multiforme (1379).

 

William Thomas Greene Morton (US) successfully anesthetized the patient Edward Abott by the administration of ether while Dr. John Collins Warren (US) removed a tumor from the patient’s neck. This operation, performed at Harvard's Massachusetts General Hospital in Boston, received widespread recognition and although it postdates William E. Clark’s and Crawford Williamson Long’s anesthetic use of ether by five years it is nevertheless considered the inauguration of the era of painless surgery (156; 2093; 2094).

Oliver Wendell Holmes (US) wrote Morton suggesting that the word anesthesia be used to refer to the process of inducing insensibility in the patient. Holmes did not coin this word; it was used by the ancient Greeks (Gk. no pain) (1198).

 

John Hutchinson (GB) developed the spirometer for measuring capacity of the lungs (940).

 

F. Wild (DE) and Karl Friedrich Wilhelm Ludwig (DE) were the first to establish an isolated heart preparation; they connected the aorta of a killed animal with the carotid artery of a living donor animal, thus maintaining perfusion of the coronary arteries of the recipient heart. This was an empty, beating, non-ejecting mammalian heart preparation. Regular beating could be maintained for a very long period of time if blood clotting was prevented (2129).

Elie de Cyon (LT-DE-RU-FR) and Karl Friedrich Wilhelm Ludwig (DE) were the first to excise a heart (frog) and to keep it in this completely isolated perfused state for a long period of time. They used it to make the first report on the effects of temperature changes on the function of the isolated (frog) heart (503). Note: de Cyon was born Ilya Fadeyevich Tsion in Lithuania.

 

Henry Newell Martin (GB-US), using the dog, was the first to devise a mammalian heart-lung preparation from which the systemic circulation was cut off and replaced by an artificial system (1212).

Oscar Langendorff (DE), using cats, rabbits and dogs, was the first to excise a mammalian heart, perfuse it, and keep it alive for several hours (1067; 1068).

Stanley J. Sarnoff (US), Eugene Braunwald (AT-US), George H. Welch, Jr. (US), Robert B. Case (US), Wendell N. Stainsby (US), and Radi Macruz (BR) used a novel blood-perfused preparation consisting of an isolated dog heart supported by a donor dog. This preparation maintained cardiac performance, allowing critical evaluation of the effect of varying heart rate, aortic pressure, and preload on oxygen consumption in the left ventricle (1662).

James R. Neely (US), H. Liebermeister (DE), Edward J. Battersby (US), and Howard E. Morgan (US), using the rat, converted the Langendorff heart into a working mode. They were able to conduct experiments for up to 3 h without deterioration in function, while manipulating ventricular pressure development, filling pressure, and heart rate and observing the effect on oxygen consumption and flow (1330). Various laboratories for studies on cardiac metabolism and coronary regulation, applying conventional chemical methods and nuclear magnetic resonance techniques, have used this preparation.

Both Sarnoff and Neely identified the importance of the tension-time index (average ejection pressure of the left ventricle multiplied by the duration of ejection) and peak systolic pressure as strong correlates of oxygen consumption, while changes in cardiac output and work were not.

 

Willard Parker (US), in 1846, performed cystotomy to relieve inflammation and rupture of the bladder (1417).

 

The third great pandemic of Asiatic cholera began (326).

 

Rudolf Ludwig Karl Virchow (DE) and Benno Ernst Heinrich Reinhardt (DE), founded a new journal, Archiv für Pathologische Anatomie und Physiologie und für Klinische Medizin [Archives of Pathological Anatomy and Physiology and of Clinical Medicine]. Later it became Virchow’s Archiv.

 

1847-1849

A dysentery (the "bloody flux") epidemic occurs in the United States (28).

1847

"One cannot say that, again with the help of the press, "the truth" can overcome the lie and the error. O, you who say this, ask yourself: Do you dare to claim that human beings, in a crowd, are just as quick to reach for truth, which is not always palatable, as for untruth, which is always deliciously prepared, when in addition this must be combined with an admission that one has let oneself be deceived! Or do you dare to claim that "the truth" is just as quick to let itself be understood as is untruth, which requires no previous knowledge, no schooling, no discipline, no abstinence, no self-denial, no honest self-concern, no patient labor!" Søren Kierkegaard (1002)

 

Lambert Heinrich Joseph von Babo (DE) discovered that the vapor pressure of a liquid is lowered by dissolving a substance in it, and that the relative lowering of the vapor pressure is proportional to the concentration of the solution (1948).

 

Marie-Jean-Pierre Flourens (FR) found that chloroform has a similar anesthetic effect to ether (696). See, Samuel Guthrie, 1831.

 

Johann Justus von Liebig (DE) isolated and named inosinic acid (Gk. inos, muscle or sinew). He obtained it from muscle extracts (2038).

Franz Haiser (DE) discovered that inosinic acid yielded hypoxanthine on acid hydrolysis, suggesting its relationship to the nucleic acids, and that it contains phosphorus as phosphate. He also concluded that there was a third component which he did not identify (823).

 

Apollinaire Bouchardat (FR) cleaved inulin and from it purified levulose (fructose) (716).

 

Johann Florian Heller (AT) was the first to note the retention of chlorides in pneumonic urine (866).

 

Johann Justus von Liebig (DE) discovered that the blood and lymph have a relatively high concentration of sodium and a low concentration of potassium while in the extravascular tissues the reverse is true (2039).

 

C. Blondeau (FR) made a study of various fermentations other than the vinous. He examined the lactic, butyric, acetic, urea, and what he called fatty fermentations. Blondeau concluded that they were all caused by vegetable growths, and suggested that different fungi caused different fermentations (186).

 

George Henry Kendrick Thwaites (GB) discovered conjugation in the diatoms and was the first to regard conjugation as a sexual process without qualification. He considered the diatoms to be algae (1853; 1854).

 

Rudolf Albert von Kölliker (CH) reported that involuntary nonstriated muscle fibers in the walls of blood vessels are elongated nucleated cells and noted that this type of cell has wide distribution within the body (2001).

Joseph Lister (GB) observed such cells in the arterioles of the frog's web and confirmed Kölliker’s findings. Lister also showed that these cells occur in mammals as well as amphibians (1126).

 

Friedrich Stein (DE) made a histological study of the female reproductive organs of beetles. This represents the beginning of the modern study of the insect ovary (1797).

 

Karl Bogislaus Reichert (DE) observed cell division of zygotes in the nematode Strongylus auriculatus and noted that the nuclear membrane disappeared prior to division. He introduced the cell theory into embryology, proving that the segments of the fertilized ovum in the mulberry stage develop into cells and that all the organs develop from these cells (1585).

 

Heinrich Frey (DE) and Karl Georg Friedrich Rudolf Leuckart (DE) separated the coelenterates from the echinoderms emphasizing that although both displayed radial symmetry they were not closely related. Leuckart denied the existence of taxons founded on negative character states and established the taxon Coelenterata (Gk. koilos, cavity, enteron, intestine). It included sponges and ctenophores (710; 711). Leuckart is commemorated by Leuckartiara Hartlaub, 1913; Podon leuckartii G.O. Sars, 1862; Protohydra leuckarti Greeff, 1870; Mesocyclops leuckarti (Claus, 1857); Sphaeronella leuckartii Salensky, 1868; Arachnomysis leuckartii Chun, 1887; Protodrilus leuckartii Hatschek, 1882; Chromadorita leuckarti (de Man, 1876); and Sabelliphilus leuckarti Kossmann, 1877.

 

Karl Georg Lucas Christian Bergmann (DE) and Joel Asaph Allen (US) independently proposed very similar rules in zoology. Bergmann’s rule states that body weight tends to a minimum in warmer regions, increases to a certain threshold as temperature declines, and then falls off again as temperature falls further. Birds and mammals in cold regions have been observed to be bulkier than individuals of the same species in warm regions. It was proposed to account for an adaptive mechanism to conserve or to radiate body heat, depending on climate. Allen’s rule states that animals adapted to cold have smaller and shorter limbs and other protruding body parts (26; 109).

 

Jean Jacques Marie Cyprien Victor Coste (FR) in his studies of various mammals, birds, and fishes discovered that partial division of the ovum begins during its descent through the oviduct (440).

 

Daniel Cornelius Danielssen (NO) and Carl Wilhelm Boeck (NO) published Om Spedalskhed (About Leprosy) which became the foundation of the modern medical history of leprosy (474).

 

Dairo Fujii (JP), in 1847, properly recorded Katayama disease (a schistosomiasis caused by Schistosoma japonicum). The report did not become available until 1909 (719).

Kenji Kawanishi (JP) described an acute disease associated with schistomiasis in a village called Katayama, located in the Hiroshima Prefecture.The syndrome of acute allergy associated with developing schistosomes is now associated with the name of this village (999).

Eitoku Mashima (JP) discovered the pathogenic agent of Japanese schistosomiasis when he found the ova of Schistosoma japonicum in the liver at autopsy (1215).

Tokuho Majima (JP) found schistosome eggs in patients with Katayama disease and associated the pathological changes with the presence of the schistosome eggs (1197).

Fujiro Katsurada (JP) discovered, described, and named the parasitic trematode Schistosoma japonicum (997; 998).

John Catto (GB) described Schistosoma cattoi, a new blood fluke of man (346).

Yoneji Miyagawa (JP) found that the young worms of Schistoma japonicum penetrate actively into the skin of the host, especially into the lymphatic spaces, and then for the most part they invade the blood capillaries or the small peripheral veins, and later gather in the right side of the heart. Some of them in the skin tissue pass by way of the lymphatic vessels to the lymphatic glands, in which latter some are arrested and killed, the others being able to pass through, finally reaching the right side of the heart. The worms in the right side of the heart now make the lung-journey following the blood stream, then return to the left side of the heart, finally reaching the branches of the portal veins in the liver by way of the aorta and the vessels of the intestinal walls, or by the arteria hepatica (1256).

Keinosuke Miyairi (JP) and Masatsugu Suzuki (JP) discovered that snails are the intermediate host of Schistosoma japonicum (1257; 1258).

 

Joseph Hyrtl (AT) published his Handbuch der Topographischen Anatomie (Handbook of Topographic Anatomy), the first textbook of applied anatomy of its kind ever issued. Other important anatomy books by him include Lehrbuch der Anatomie des Menschen, Handbuch der Praktischen Zergliederungskunst als Anleitung zu den Sectionsubungen und zur Ausarbeitung, Anatomischer Praparate, and Onomatologia Anatomica. While Chair of Anatomy at the University of Vienna he achieved worldwide fame as a teacher (959-962).

 

Ernst Wilhelm Brücke (DE) discovered a ciliary muscle later named for him (313).

 

Alexander Ecker (CH) described erythrocytes inside of rabbit spleen cells. He speculated that the red cells might have been "jammed" into the larger cells by circulatory forces (608).

 Kranid Slavjansky () reported the presence of coal dust within alveolar macrophages from miners’ lungs (1762).

Nathanael Lieberkühn (DE) showed that leukocytes could ingest erythrocytes (1119).

Felix Victor Birch-Hirschfeld (DE) found that leukocytes might take up bacterial cocci injected into the blood stream (165).

William Osler (CA) found erythrocyte-containing leukocytes in the blood of humans with various diseases (1377).

Giulio Cesare Bizzozero (IT) described the clearance of carbon particles by cells of lymph nodes and the internalization of small leukocytes by larger leukocytes in the spleen (176). Verify

Giulio Cesare Bizzozero (IT) described the process of efferocytosis in an experimental model performed in the inflamed anterior chamber of the eye of rabbits. He described the pus: "If one investigates the exudate, it is possible to detect, in addition to the white blood suppurative cells, different bigger cellular elements (30–50 μm) and containing in addition to the nucleus a variable number (from 1 to 20 and more) of round corpuscles, which in terms of morphology and chemical reaction look exactly like the other suppurative cells." (177)

He concluded his second report by stating: "In summary, my observation showed the presence of big cells able to engulf white blood suppurative cells or red blood cells in their contractile protoplasms. This represents a way through which the pus or blood is absorbed from the anterior chamber." (178)

 

James Young Simpson (GB) and Jacob Bell (GB) introduced the use of chloroform as an anesthetic in obstetrics. After testing it on themselves it was given to a woman during childbirth (1753; 1754; 1756).

 

Jean-Marc Dupuy (FR) and Nikolai Ivanovich Pirogov; Nicholas Ivanovich Pirogoff; Nikolai Iwanowitsch Pirogow (RU) discovered rectal anesthesia (583; 1472; 1508).

Daniel Molière (FR) and Alex Iversen (DK) reintroduced rectal anesthesia (1261).

 

Robert Bentley Todd (IE-GB), in 1847, coined locomotor ataxy (tabes dorsalis), by which he began the separation and classification of spinal diseases. Prior to this all spinal diseases were labeled paraplegias.

"Two kinds of paralysis may be noticed in the lower extremities, the one consisting simply in the impairment or loss of voluntary motion, the other distinguished by a diminution or total loss of the power coordinating movement. In he later form, while considerable voluntary motion remains, the patient finds great difficulty in walking and his gait is so tottering and uncertain that his centre of gravity is easily displaced… In two examples of this variety of paralysis I ventured to predict disease of the posterior columns, the diagnosis being founded upon the views of their functions which I now advocate, and this was found to exist on a postmortem inspection; and in looking through the accounts of recorded cases in which the posterior columns were the seat of the lesion, all seem to have commenced by evincing more or less disturbance of the locomotive powers, sensation being affected only when the morbid change of structure extended to and more or less involved the posterior roots of the spinal nerves." (1861)

 

Jaques Francois Édouard Hervieux (FR) reported on autopsies of 44 jaundiced infants. His descriptions of pathoanatomical findings were very detailed and systematic. A number of his clinical observations are still thought to be accurate today, such as the essentially benign nature of neonatal jaundice in most cases, the appearance of neonatal jaundice during the first 2 to 4 days of life as well as its disappearance within 1 to 2 weeks, and the cephalocaudal progression of jaundice. He described jaundice of the brain in 31 of his 44 autopsied cases, with variable intensity of staining (890). Christian George Schmorl (DE) would name this yellow staining of certain areas of the brain kernicterus (1688).

 

Golding Bird (GB) and John Hilton (GB) recorded the first operation for intestinal strangulation of the small intestine. No anesthetic was used; the patient died nine hours afterward (167).

 

Moritz Schiff (DE) investigated the effects of section of the vagus on respiration (1678).

 

Hundreds of Cayuse Indians in the Pacific Northwest were killed by measles. This tribe had never been exposed to measles previously, and missionaries were blamed for introducing it. One missionary near present-day Walla Walla tried to provide them with food and medicine, but the Indians thought he was making it worse, and killed him, his wife and twelve others at the mission, and took several others hostage. Several years of conflict followed (1030).

 

Archiv für Pathologische Anatomie was founded.

 

The American Medical Association was founded then reorganized in 1901 (1198).

 

David Jones Peck (US) was the first African-American man to receive the Doctor of Medicine degree from a United States medical school (Rush Medical College).

 

1848

"Science is the labor of mind applied to nature." Friedrich Wilhelm Heinrich Alexander von Humboldt (DE) (1994).

 

“In considering the study of physical phenomena, not merely in its bearings on the material wants of life, but in its general influence on the intellectual advancement of mankind, we find its noblest and most important result to be a knowledge of the chain of connection, by which all natural forces are linked together, and made mutually dependent upon each other; and it is the perception of these relations that exalts our views and ennobles our enjoyments.” Friedrich Wilhelm Heinrich Alexander von Humboldt (DE) (1994).  

 

Auguste Laurent (FR) and Charles Frédéric Gerhardt (FR) indicated that leucine and glycine have very similar chemical properties and they considered both to be part of a homologous series having the composition CnH2n+1NO2 (1079).

 

Benjamin Collins Brodie, Jr. (GB) determined that beeswax consists primarily of the solid alcohols: cerotic, myricine, and ceroleine (290-292). This work proved the existence of solid alcohols that were homologous with known liquid alcohols, and it had important implications for the understanding of animal metabolism.

 

Louis Pasteur (FR), while professor of physics in Dijon, made the discovery that formed the starting point for all his later brilliant studies in bacteriology, viz., that by means of fermentation he could produce, then separate, the two varieties of tartaric acid distinguished by their capacity to rotate a plane of polarized light in opposite directions. He had already separated these types on a crystallographic basis (1844). One form, he found, was destroyed by fermentation, the other not. This discovery opened the way for a more exact understanding of the essential nature of ferment action. From this he developed an intense interest in the subject of fermentation in general (1422-1424).

 

Rudolf Albert von Kölliker (DE) proved the act of phagocytosis beyond doubt when he observed the heliozoan, Actinophrys sol. in the act of ingesting another protozoan (2004).

 

Per Henrik Malmsten (SE) discovered that Trichophyton tonsurans causes a human dermatomycosis (1200).

 

Claude Bernard (FR) and Charles-Louis Barreswil (FR) suggested that the production of glucose by the liver (glycogenesis) is a characteristic of mammalian life and occurs independently of whether glucose is in the diet. They concluded that "like plants, animals can create and destroy sugar physiologically." (119; 124-126; 136)

Bernard suggested, based on experiments with the hypothalamus, that glucose homeostasis is regulated by a balance of opposing influences originating in the central nervous system (122).

Lawrence A. Frohman (US), Lee L. Bernardis (US), Errol B. Marliss (CA), Lucien Girardier (CH), Josiane Seydoux (CH), Claes B. Wollheim (CH), Yasunori Kanazawa (JP), Lelio Orci (CH), Albert Ernst Renold (CH), and Daniel Porte, Jr. (US) showed that while Bernard was not completely correct the islets of Langerhans are richly endowed with autonomic nerve endings containing both adrenergic and cholinergic synaptic vesicles, and gap junctions between nerve endings and islet cells have been observed. Stimulation of the ventromedial nucleus of the hypothalamus and of the sympathetic nerve to the pancreas causes an outpouring of glucagon and limits insulin secretion, as does the administration of catecholamines. Appropriate adrenergic blocking agents block these effects. The effect of adrenergic stimulation is to lower the insulin: glucagon ratio, thereby increasing hepatic glucose production and glycemia (715; 1211).

 

Richard Owen (GB) coined the word homology in its biological context, defining it as "the same organ in different animals under every variety of form and function" and elaborated between homology (homologous) and analogy (analogous) as applied to the anatomical features of animals. He emphasized that all vertebrates have a similar structure, which has adapted in different ways but was not "aimed" in a linear way at man (1388; 1389; 1392).

 

Johann Florian Heller (AT) invented the ureometer for determining specific gravity of urine samples (730).

 

Emil Heinrich du Bois-Reymond (CH-DE) refined old instruments and devised new ones with which he detected the passage of tiny currents in nerve and muscle (e.g., nerve galvanometer). He was able to show that the nerve impulse is accompanied by a change in the electrical condition of the nerve (556).

 

Carl Müller (DE) brought together in one publication the entire world’s mosses, which had been described. It included 2,400 species of Mucsi and only 25 species of Sphagnum (1294).

 

Joseph Dalton Hooker (GB) realized that planktonic diatoms are plants and suggested that they play the same ecological role in the sea that green plants do on land (910).

 

Matthias Jakob Schleiden (DE) published Die Pflanze und ihr Leben, a book that dramatically changed the approach to botany. Microscopic examination was exalted, giving it an importance equivalent to that of systematics. Inductive reasoning was elevated in importance to exceed that of philosophical theorizing (1683).

 

Henri Milne Edwards (FR), based upon respiratory organs, divided the gastropoda into Pulmonés, Opisthobranches, and Prosobranches, now rendered Pulmonata, Opisthobranchia, and Prosobranchia (609).

Johann Wilhelm Spengel (DE), based upon their nervous system, divided the gastropoda into Strepyoneura and Euthyneura. Streptoneura is equivalent to Prosobranchia; Euthyneura embraces Opisthobranchia and Pulmonata (1776).

Edwards is commemorated by Edwardsia de Quatrefages, 1841; Edwardsiella Andres, 1883; Henricia J.E. Gray, 1840; Autolytus edwardsi de Saint-Joseph, 1887; Lophoura edwardsi Kölliker, 1853; Plesiopenaeus edwardsianus (Johnson, 1867); Plesionika edwardsii Brandt, 1851; Dynamene edwardsi Lucas, 1849; Grapsicepon edwardsi Giard & Bonnier, 1888; Glossocephalus milneedwardsi Bovallius, 1887; Onisimus edwardsii Krøyer, 1846; Diastylis edwardsi Krøyer, 1841; Neoamphitrite edwardsii de Quatrefages, 1866; Colpaster edwardsi Perrier, 1882; Milnesium Doyère, 1840; Jasus edwardsii (Hutton, 1875), Odontozona edwardsi (Bouvier, 1908), Milneedwardsia Bourguignat, 1877, Boeckosimus edwardsii (Krøyer, 1846), Lithophyllon edwardsi (Rosseau, 1850), and Goniastria edwardsi Chevalier, 1971. Phascolosoma spengeli, and Spengelia Willey, 1898, commemorate Spengel.

 

William Sharpey (GB) described Sharpey's fibers (bone fibers, or perforating fibers) as a matrix of connective tissue consisting of bundles of collagenous fibers connecting periosteum to bone. They are part of the outer fibrous layer of periosteum, entering the outer circumferential and interstitial lamellae of bone tissue (1528). Note: Clementi (IT) claimed priority in this discovery.

 

Hermann Ludwig Ferdinand von Helmholtz (DE) demonstrated that heat production accompanies contraction in muscle (1985).

 

Henry Bence Jones (GB) discovered an unusual protein (albumose) in the urine of patients with softening of the bones-myelopathic albumosuria(979).

Charles Freeman Geschickter (US) and Murray Marcus Copeland (US) found a myeloma protein in serum and distinguished it from the urinary Bence Jones proteins (747).

 

Alfred Baring Garrod (GB) noted the link between gout and hyperuricaemia (731).

 

Josiah Clark Nott (US) advanced the hypothesis that yellow fever is unique and that insects vector it from person to person (1362).

 

Rudolf Ludwig Karl Virchow (DE) first described a lymph node in the left supraclavicular fossa (the area above the left clavicle) as being associated with gastric cancer (1926). The finding of an enlarged, hard node (also referred to as Troisier's sign for Charles Emile Troisier (FR)) has long been regarded as strongly indicative of the presence of cancer in the abdomen, specifically gastric cancer, that has spread through the lymph vessels (1880). Note: This lymph node is called Virchow node or signal node.

 

Johann Ferdinand Martin Hayfelder (DE-RU) was the first German surgeon to use an anesthetic, performing many operations using ether or chloroform. He discovered the anesthetic properties of ethyl chloride (894).

 

John M. Harlow (US) reports a singular incident, which was to change our understanding of the relation between mind and brain. Phineas P. Gage, a 25-year-old railroad foreman, was excavating rock. In preparation for blasting he was tamping powder into a drill hole when a premature explosion drove the tamping iron—1.1m long, 6 mm in diameter, and weighing 6 kg—through his left cheek and out of the vault of his skull with such force that it threw him on his back and fell several rods behind, "smeared with brain." Despite his injuries he remained conscious and only a few minutes later was sitting in an ox cart writing in his workbook. He recognized and reassured Dr. Harlow, who had been summoned to the scene. The wound continued to bleed for two days; then followed a virulent infection that rendered Gage semiconscious for a month. His condition was so poor that a coffin had been prepared. Nevertheless, Dr. Harlow continued treatment, and by the fifth week the infection had resolved, and Gage had regained consciousness. He later developed epilepsy, and in May 1861, 12 years after the injury, he died in status epilepticus.

Immediately after physical recovery Dr. Harlow described Gage as follows: “Remembers passing and past events correctly, as well before as since the injury. Intellectual manifestations feeble, being exceedingly capricious and childish, but with a will as indomitable as ever; is particularly obstinate; will not yield to restraint when it conflicts with his desires.” Dr. Harlow reports that Gage’s employers, “who regarded him as the most efficient and capable foreman ... considered the change in his mind so marked that they could not give him his place again.... He is fitful, irreverent, indulging at times in the grossest profanity (which was not previously his custom), manifesting but little deference for his fellows, impatient of restraint or advice when it conflicts with his desires.... A child in his intellectual capacity and manifestations, he has the animal passions of a strong man.... His mind was radically changed, so decidedly that his friends and acquaintances said he was 'no longer Gage.' (832; 833)

 

Emil Heinrich du Bois-Reymond (CH-DE), using a sensitive galvanometer, described an "action potential" accompanying muscular contraction. He detected the small voltage potential present in resting muscle (resting currents) and noted that this diminished with contraction of the muscle (555).

 

The American Association for the Advancement of Science established Science Magazine, which would become one of the world's foremost science journals.

 

Zeitschrift für Wissenschaftliche Zoologie was founded.

 

1849

"The philosophical study of nature endeavors, in the the vicissitudes of phenomena, to connect the present with the past." Friedrich Wilhelm Heinrich Alexander von Humboldt (DE) (1995)

 

Charles Adolphe Würtz (FR) discovered the aliphatic amines by the action of alkali on cyanic and cyanuric esters (1421).

 

Eugène Auguste Nicolas Millon (FR) introduced a color test for the presence of protein, which bears his name. The reagent was prepared by dissolving mercury in fuming nitric acid and diluting with water. When the reagent was heated in the presence of most proteins it gave a brick-red precipitate (1250).

Otto Nasse (DE) discovered that a positive Millon Test is not confined to proteids and to tyrosine but is a general reaction to all aromatic bodies in which a hydroxyl group is connected with the benzol ring (1326; 1327).

 

Wilhelm Friedrich Karl August Fürst zu Salm-Horstmar (DE) developed a totally inert soil by charring crystalline sugar and demonstrated that normal plants could be grown on this soil provided they were supplied the proper inorganic salts (1654-1656).

 

Alexander Carl Heinrich Braun (DE) helped modify the cell theory by insisting that the plant cell wall is in fact a structure, which entombs the true living part of the cell where physiological activities take place. The wall must give way if the cell is to rejuvenate (reproduce) itself (240; 241; 869).

 

Henri Victor Regnault (FR) and Jules Reiset (FR) were the first to devise a closed system for respiration studies with which they refined Lavoisier’s experiments on measuring the oxygen uptake and carbon dioxide production of animals and calculated the first accurate ratios of carbonic acid gas given off divided by oxygen absorbed. This relationship became known as the respiratory quotient (1581).

 

Claude Bernard (FR) discovered that pancreatic juice and bile performs some function, which is indispensable for the digestion and absorption of fat. This he conceived to be digestion of fat into glycerol and fatty acid, the latter forming soaps, soluble in water and capable of passing the water-permeated membrane of the intestinal villi (120; 121; 128; 129; 131).

Arpad Bokay (HU) gave the first indication of the existence of enzymes in pancreatic juice hydrolyzing lecithin into glycerophosphoric acid, fatty acids and choline (192).

 

Claude Bernard (FR) is credited with originating and applying the phrase internal secretion as it applied to the liver liberating glucose into the bloodstream (126). See, Brown-Séquard, 1856.

 

Heinrich R. Göppert (DE) and Ferdinand Julius Cohn (DE) used carmine and madder (Galium, Ladies’ broomstraw; contains alizarin) with the intention of making the internal contents of Nitella flexilis (alga) more visible (774).

 

Rudolf Albert von Kölliker (CH) showed that nerve fibers are elongated portions of nerve cells (2005). See, Robert Remak, 1838.

 

Hermann Ludwig Ferdinand von Helmholtz (DE) declared nervous transmission to be a material process capable of being measured—invented the myograph—then using the frog made the first measurement of the conduction velocity of a motor nerve axon, finding it to be about one-tenth the speed of sound (1986; 1988).

Julius Bernstein (DE) confirmed the work of Helmholtz by measuring the time course of the action potential (a phrase coined by him) and producing the first plot of the action potential with the wave moving in both directions from the point of stimulation and with an overshoot (137).

 

Thomas Addison (GB) described patients showing clinical features attributable to adrenal insufficiency: anemia, debility, feebleness of the heart, irritability of the stomach, and changes in skin color. This would become known as Addison's disease (11-13). Note: Some consider this to be the first hormonal disorder to be identified.

Armand Trousseau (FR) suggested that the disease should bear Addison's name (1883). The condition is commonly called adrenocortical insufficiency or Addison’s disease to this day. Causes of adrenal insufficiency can be grouped by the way they cause the adrenals to produce insufficient cortisol.

 

Franz Antoine Pollender (DE), in 1849, observed the microorganism now known as Bacillus anthracis (Gr. anthrakos, meaning coal and referring to the black escher in the cutaneous form of the disease) in the blood and organs of infected animals. He thought they were of a vegetable nature. He did not report his finding until 1855 (1487).

Pierre-Francois-Olive Rayer (FR) inoculated sheep with blood of other sheep dead of anthrax. Microscopically he saw the anthrax bacillus in the blood of the inoculated sheep (1575).

Casimir Joseph Davaine (FR) reasoned that bacteria must be the cause of anthrax because they are consistently present in the disease, the disease can be transmitted by inoculation, and when the bacteria are absent there is no anthrax (479-486).

Heinrich Hermann Robert Koch (DE) traveled to Breslau, Germany to share his discovery of the complete life cycle of the anthrax bacillus, Bacillus anthracis, with Ferdinand Julius Cohn and other prominent German scientists reasoning that the facts about anthrax suggested the possibility of spore formation. Studying the disease in small animals and under primitive conditions in his home he had found that it was transmissible from mouse to mouse in a series of twenty generations, and that the lesions in each member of the series were identical. He worked out the distribution of the bacilli in the bodies of different animals. Placing minute particles of fresh anthrax spleens in drops of sterile blood serum or aqueous humor, he set himself to watch, hour after hour, what took place. His technique was simplicity itself; his apparatus was homemade. After twenty hours he saw the anthrax rods grow into long filaments especially at the edge of the cover glass, and, as he watched, he saw rounded and oval granular bodies appear in the filaments. The bodies became clearer and stood out in the filaments. He realized that they were spores, which had not been seen before. He determined accurately the optimal thermal conditions for spore formation. He succeeded in showing that under suitable conditions the spores again grew into typical anthrax rods. He described and correctly interpreted germination of spores and determined that they are highly resistant to adverse conditions. He realized the epidemiological significance of the resistant spores. He studied septicemia and demonstrated that it and anthrax are different processes. In putrid infusions of vitreous humor he found a bacillus almost identical in appearance as in spore production with the true anthrax bacillus but showed that it does not produce anthrax. From facts such as these he concluded, "Only one kind of bacillus is in position to induce the specific morbid process whereas other schizophytes are not, or, if pathogenic, they act in a manner different from anthrax." He tried to induce anthrax in mice by having them ingest the organism but was unsuccessful. He confirmed Brauell’s observation that the young of pregnant animals, dead from anthrax, are non-infective, and he studied the general pathology of experimental anthrax in a complete and masterly manner. He showed that the septicemic stage is reached only very late in anthrax of mice. Dogs, partridges, and sparrows were not susceptible to the disease (326; 1020).

Heinrich Hermann Robert Koch’s (DE) culturing of the anthrax bacillus on the aqueous humor of an ox’s eye, description of its life history, and reproduction of the disease state with an axenic culture of the microorganism is one of the most significant events in the history of biology and medicine. It represents among other things the first proof that a specific disease, anthrax, can be caused by a specific microorganism.

Heinrich Hermann Robert Koch (DE) purified anthrax by passing it through a series of otherwise healthy animals (1020).

Heinrich Hermann Robert Koch (DE) found that when dealing with non-pigmented organisms and pathogens, broths based on fresh beef serum or meat extracts (bouillions) gave the best bacterial growth (1020).

 

John Snow (GB) concluded that fecal pollution and transfer of the unknown specific agents from the sick to the well through direct contact and through drinking water were responsible for the spread of cholera. His cholera experience began at the age of eighteen years during the first pandemic of the disease, when he saw the miners brought up from some of the coal-pits of Northumberland in the winter of 1831-32, after having profuse discharges from the stomach and bowels, and when fast approaching a state of collapse. In answer to Snow’s inquiry, a relative connected with a colliery near Leeds wrote: "The pit is one huge privy and of course the men," who spend eight to nine hours in the pits, "always take their victuals with unwashed hands." He came to his conclusion through care of patients, painstaking follow-up of case after case, searching for the unknown contacts with earlier cases, and from statistical studies.

The culmination of his studies in the disease came as a result of the Broad Street pump epidemic in London in 1854. There were cases of cholera in London during the year but few in this area until the latter part of August. Within 250 yards of the pump in a two-week period from August 31, upwards of five hundred fatal cases of Asiatic cholera occurred. By laborious questioning Snow found that most of the cases were among people in the habit of drinking from the Broad Street pump and that few of those in the neighborhood who used other water acquired the disease. Further studies gave convincing evidence: the brick work of the well and nearby cesspool—only 2 feet 8 inches away! —was in a badly decayed condition and the well was at a lower level showing drainage from cesspool to the well. It is in this work that he originated the concept of the well carrier (1769; 1770; 1772).

John Snow (GB) wrote, "…[T]he disease is communicated by something that acts directly on the alimentary canal, the excretions of the sick at once suggest themselves as containing some material which, being accidentally swallowed, might attach itself to the mucous membrane of the small intestines, and there multiply itself…" (1770)

 

Heinrich Hermann Robert Koch (DE) while studying cholera in Egypt demonstrated the importance of the concept of the well carrier first proposed by John Snow (1025).

 

G. Gros (RU) discovered a parasitic amoeba in the tartar of the teeth (likely Endamoeba gingivalis). This possibly represents the first discovery of a parasitic amoeba living as a parasite on man (801).

 

Thomas Henry Huxley (GB) wrote one of the first scholarly papers on the medusa. He noted: a lack of blood and blood vessels, a body plan composed of two tissue layers (ectoderm and endoderm) physiologically analogous to the serous and mucous layers in a typical embryo, that the Medusae, Hydroids, and Sertularian polyps possess body plans similar enough to justify their being grouped together, and that some of these Coelenterata are complicated colonial forms of typical medusae double-membrane structures, i.e., Portuguese man-of-war (941; 950).

 

Arnold Adolph Berthold (DE) observed that the atrophy of a rooster’s comb, which normally follows castration, could be prevented if the testes were transplanted to another location in the rooster. Berthold realized that there was some type of gonadal secretion involved, "…. so, it follows that the interaction in question is exerted through the productive relationship of the testes, that is, through their action on the blood and next through a consequent action of the blood on the organism as a whole…" (140) The real significance of testicular secretion into the blood became evident and thus Berthold should be viewed as the founder of the concept of endocrine function. See, Théophile de Bordeu, 1775.

 

Robert Bentley Todd (IE-GB), in 1849, described epileptic hemiplegia, which came to be called Todd's paralysis (1864).

 

Charles Emmanuel Sédillot (FR) performed the first successful gastrostomy (creation of an artificial gastric fistula). The patient died within a few hours (1730).

 

Edgar Allan Poe (US) most likely died of complications brought on by severe alcoholism (1174).

 

The third major pandemic of cholera, again starting in Bengal, reached Europe and the U.S. in 1848-49. In 1854, the worst year, 23,000 died in Britain alone. In that same year, British physician John Snow succeeded in identifying contaminated water as the transmitter of the disease, a breakthrough in eventually bringing cholera under control (1030; 1337).

 

Elizabeth Blackwell (GB-US) was the first woman in the modern era to receive a medical degree. Blackwell pursued medical studies independently beginning in 1844. In 1847, she began studying under the tutelage of Dr. Samuel H. Dickson, a professor at Charleston Medical College. Although she applied to more than 25 medical schools, only Geneva Medical School (now Hobart and William Smith Colleges) accepted her. She endured much ridicule and prejudice but still graduated at the head of her class in 1849. Blackwell established a practice in New York but by 1869, she had decided to settle permanently in England (2142).

 

William Bowman (GB) authored the first work to include a sound description of the microscopic anatomy of the eye and the ciliary ('Bowman's') muscle (228).

 

Henry Willard Williams (US), in 1849, introduced the use of sutures for cataract surgery by using a shortened sewing needle threaded with a strand of fine glover's silk (2137).

 

Ludwig Traube (DE) and Friedrich Wilhelm Felix von Bärensprung (DE), in 1849 or 1850, introduced measurement of body temperature as a routine clinical examination method (1874).

 

Guillaume Benjamin Amand Duchenne de Boulogne (FR) and Francois-Amilcar Aran (FR) described a condition— Aran-Duchenne spinal muscular atrophy— characterized by chronic progressive wasting of muscles with subsequent weakness and paralysis (41; 561; 562).

Jules Bernard Luys (FR) later found this condition to be caused by degeneration of the anterior horn cells of the spinal cord (1161).

 

Luther V. Bell (US) reported an affliction characterized by wild delirium, general disturbances of the psychic functions and of the motor functions and fever. He found slight cerebral and meningeal engorgements on autopsy (99). Note: Called acute peripheral encephaliys, phrenitis mania gravis, typhomania, acute delirium, delirium grave, and Bell’s disease it is caused by acute hyperemia and subsequent inflammatory changes in the cerebral cortex.

 

Robert William Smith (IE) wrote a treatise on cases of generalized neurofibromatosis (von Recklinghausen’s disease) 33 years before Recklinghausen’s account (1767).

Friedrich von Recklinghausen (DE) reported on cases of multiple fibroma and multiple neuroma (2055). Note: Ribera (IT) may have been the first to observe neurofibromatosis. Johann Gotfried Rheinhardt (DE) in1793, observed neurofibromatosis.

 

James Young Simpson (GB) introduced into obstetrical practice the long obstetrical forceps (1757), and the use of iron wire sutures (1759).

 

c. 1850

Emmanuel Geoffroy (FR) isolated nicouline (rotenone) from a specimen of Robinia nicou, now called Lonchocarpus nicou, while traveling in French Guiana. This was posthumously published in 1895 (744).

The earliest recorded insecticidal use of rotenone was against leaf-cutting caterpillars in 1848 (1658; 2091).

Kazuo Nagai (JP) isolated the active principle from Derris chinensis and named it rotenone (1321).

Frederick B. La Forge (US), Herbert L. Haller (US), and Lloyd E. Smith (US) determined the chemical structure of rotenone (1056).

Youssef Hatefi (US) found that rotenone works by interfering with the electron transport chain in mitochondria. Specifically, it inhibits the transfer of electrons from iron-sulfur centers in complex I to ubiquinone. This prevents NADH from being converted into usable cellular energy in the form of ATP (849).

 

 

The third plague pandemic began in China in the 1850s and spread slowly until it reached the seaports in the 1880s, then spread more rapidly around the world, striking particularly hard in India, Egypt and North Africa, and South America. The continental U.S. was largely spared, but Hawaii suffered a severe outbreak in 1899, and San Francisco was affected in 1900-1904, and again in 1907-1909. The second outbreak in San Francisco was exacerbated by unsanitary conditions following the earthquake of 1906. Sporadic outbreaks continued worldwide for years, and officially this pandemic was not considered over until 1959 (1030).

 

1850

"There rolls the deep where grew the tree,

O Earth what changes hast thou seen!

There where the long street roars, hath been

The stillness of the central sea." In Memoriam by Alfred Lord Tennyson

 

Rudolf Julius Emanuel Clausius (PL-DE) noted that heat is destructible and examined how it can be converted to work with the flow of heat from a warm body to a cold one. He concluded that entropy, a concept he introduced, must inevitably increase in the universe (379-381; 1188).

 

Max Schulze (DE) discovered a solution of zinc chloride-iodine-potassium iodide in water useful for testing for cellulose, which it colors blue (1713). Note: Schulze’s reagent

Ludwig Radlkofer (DE), in 1855, published precise instructions for preparation of Schulze’s reagent (1536).

 

Harry Stephen Meysey Thompson (GB) and John Thomas Way (GB) invented ion-exchange chromatography and ion chromatography methods when they treated various clays with ammonium sulfate or carbonate in solution to extract the ammonia and release calcium (1832; 2097). In 1927, the first zeolite mineral column was used to remove interfering calcium and magnesium ions from solution to determine the sulfate content of water.

Edward R. Tompkins (US), Joseph X. Khym (US), Waldo E. Cohn (US), Warren C. Johnson (US), Laurence L. Quill (US), and Farrington Daniels (US) initiated the modern era of ion-exchange chromatography and ion chromatography in their quest to purify radioisotopes and rare earths for the Manhattan Project. A technique was required to separate and concentrate the radioactive elements needed to make the atom bomb. Researchers chose adsorbents that would latch onto charged transuranium elements, which could then be differentially eluted. Ultimately, once declassified, these techniques made available new ion exchange resins to develop the systems that are often used today for specific purification of biologicals and inorganics (976; 1865).

Waldo E. Cohn (US) then applied the technique to separation of mononucleotides (399).

 

Ludwig Ferdinand Wilhelmy (DE) described the first measurements of the velocity of a chemical reaction in a homogeneous medium; he polarimetrically determined the rate of inversion of cane sugar in the presence of various acids (2131).

 

Adolf Friedrich Ludwig Strecker (DE) synthesized alanine via the cyanohydrin reaction from acetylaldehyde. He proposed the term alanine, which contains the first syllable of the word aldehyde (1815). Alanine is also called aminopropionic acid.

Paul Schützenberger (FR) was the first to find alanine in proteins (silk and ovalbumin) (1714; 1715).

Theodor Weyl (DE), in 1888, isolated alanine from acid hydrolysis of silk fibroin (2120).

 

Jean Baptiste Joseph Dieudonné Boussingault (FR) was the first to devise a methodology whereby nitrogen of ammonia salts and nitrogen as urea could be distinguished in urine (219).

 

Charles Adolphe Würtz (FR) obtained methylamine by treating casein with alkali (2173).

 

Johannes Petrus Müller (DE) described echinoderm metamorphosis (1313).

Johannes Petrus Muller (DE) described "Muller's larva" of polyclad turbellarians and observed its metamorphosis into the juvenile worm (1313).

 

Johann Joseph Scherer (DE) isolated and named sarkin (hypoxanthine) from the pulp of the spleen and heart muscle (1677). Hypoxanthine was later shown to be a secondary product formed by the deamination of adenine.

 

Franz von Leydig (DE) discovered the interstitial cell in the seminiferous tubules and in the mediaseptum and connective tissue septa of the testes (2017; 2019). These cells, called Leydig’s cells, are believed to produce the male hormone testosterone, which determines male secondary sexual characteristics.

 

Richard Owen (GB), in 1850, described 'a small, compact yellow glandular body attached to the thyroid' during the dissection of an Indian rhinoceros. Note: Doubtless the parathyroid gland (1391).

Ivar Sandström (FI) described what he called the ‘glandulae parathyroidae’ in the dog, cat, rabbit, ox, horse and man (1661).

William Stewart Halsted (US) noticed the ‘disastrous results from the loss of the glands’ and the resulting tetany, as did Billroth (828; 2162).

Alfred Kohn (CZ) by means of preparing numerous serial sections through the thyroid gland and adjacent organs of the neck in humans and several mammals, Kohn demonstrated the presence of four parathyroids in cat and rabbit: two external parathyroids and two parathyroids within the thyroid gland, one within each lobe (1027; 1028).

Giulio Vassale (IT) and Francesco Generali (IT) demonstrated in their first animal experiments that removal of all parathyroid glands in cat and dog, leaving the thyroid gland intact, ended in fatal tetany in both species (1909; 1910).

Marcel Eugène Émile Gley (FR) observed that the tetany and death caused by experimental thyroidectomy in dogs occurred only if the excised material included the glands described by Sandström (749).

Max Askanazy (CH) published the first report of a patient with bone disease associated with a large parathyroid tumor (52).

Jacob Erdheim (AT) documented that patients who died with osteomalacia and osteitis fibrosa cystica had enlarged parathyroid glands at autopsy (629). Note: His conclusion that their enlargement resulted from compensatory hyperplasia was erroneous, but his observation was key in making the association between bone disease and abnormalities of the parathyroid glands.

William Maccallum (US), Carl Voegtlin (US), and K.M. Vogel (US) initially felt that the parathyroids neutralised a circulating toxin, but subsequent careful experimentation led them to the correct conclusion that the parathyroids controlled calcium metabolism. They demonstrated that the low blood calcium (hypocalcaemia) and tetany that followed removal of the parathyroids could be controlled by calcium administration, concluding that parathyroid tetany was a direct result of calcium deficiency (1168-1170).

Friedrich Schlagenhaufer (AT) suggested that an enlarged parathyroid might be the cause of bone disease and not the result of it (1679).

John Bland-Sutton (GB), c. 1917, carried out an intentional parathyroidectomy for a parathyroid tumor. operating on woman with airway obstruction: “I removed the rounded body, as big as a cherry, situated below the lower angle of the thyroid gland on the left side of the trachea. It had the microscopic features of a parathyroid.” ref

Adolf M. Hanson (US) developed a method of extracting a stable substance (hormone) from bovine parathyroid glands that could successfully treat tetany (829).

James Bertram Collip (CA) was also convinced that the parathyroid glands contained a calcium-regulating hormone. He independently developed his own technique for extracting this substance and testing it clinically. He named it parathyrin (408-411).

 

Augustus Volnay Waller (FR-GB) and Julius Budge (DE) contrasted degenerating nerves with unaffected nerves and demonstrated that when nerve fibers are cut the distal portions of the fiber degenerate. This made it possible to trace the course of fibers through the nervous system and demonstrated the importance of the nucleus in the regeneration of fibers. He also found that healthy nerve cells swell in water while damaged cells lost this power (possibly the first account of the loss of semi-permeability in a damaged cell) (2080-2082; 2086; 2088; 2089).

 

Georg Liebig; Georg von Liebig (DE) published a study on isolated frog muscle activity under aerobic and anaerobic conditions in which he concluded… "in the process of respiration the blood really acts only as a means to effect the transport of gases to the capillaries and back, and that in the capillaries there occurs not the formation of carbonic acid, but only the exchange through the walls of the blood vessels of that already formed for the oxygen of the blood … [T]he formation of carbonic acid from a part of the respiratory oxygen … proceeds in the body not within the capillary vessels, but outside them in the muscle tissue." (1120)

 

Karl Friedrich Wilhelm Ludwig (DE) and Friedrich Wilhelm Noll (DE) proposed that lymph is formed by the diffusion of fluids from the blood vessels through the vessel walls into the surrounding tissues, the motor power being the capillary blood pressure. The ultimate cause of the lymph flow must be looked for in the energy of the heart’s contraction (1352).

 

Claude Bernard (FR) and Théophile-Jules Pelouze (FR) showed that curare destroys the communication between nerve and muscle but that the muscle still responds to stimuli (123; 1446). Bernard went on to show that curare selectively abolishes the action of the motor nerves by stopping the transmission of impulses from motor nerves to voluntary muscles but has no effect on the sensory nerves (130; 132).

Edme Félix Alfred Vulpian (FR) demonstrated that curare interrupts the communication between the nerve fibers and the muscle fibers and does not act on the central nervous system (2066).

 

Franz von Leydig (DE) reported intracellular microorganisms within the cells of aphids and scale insects (he did not realize what they were). This was the first study of this type (2016; 2018). These intracellular collections of microorganisms have been called symbiotic organs, pseudovitelli, green bodies, bacteriosomes, mycetoms, and mycetomes.

F. Blochmann (DE) was the first to be convinced that mycetomes contain bacteria (183-185).

Paul Buchner (DE) recognized that the bacteria of mycetomes are symbiotic with their host (317).

 

Ferdinand Julius Cohn (DE) described the developmental and sexual cycles of the algae Protococcus pluvialis (1850), Stephanosphaera pluvialis (1856), Sphaeroplea annulina (1855), and Volvox globator (1875) and of the lower fungi Pilobolus crystallinus and Empusa (Entomophthora) muscae (392; 393; 395-398).

 

Pierre-Joseph van Bénéden (BE) deduced that bladderworms (cystici), which had hitherto been regarded as a separate class of helminths, were simply larval tapeworms. He coined the terms scolex and strobila in reference to tapeworms (1902).

 

Maximillian Johann Sigismund Schultze (DE) was the first to render a correct account of the general anatomical features of the nemertines. Schultze knew the use of the proboscis, distinguished between the armed and unarmed types of proboscis, discovered the nephridia, and defined the nemertines—which he called Rhynchocoela or Nemertina—as turbellarians with an anus and an eversible proboscis (1705). These animals are commonly called ribbon worms.

 

Félix Dujardin (FR) identified and described the mushroom bodies (corpora pedunculata) in the hymenopteran brain (bee, bumblebee, sphex, ant, fruitfly Drosophila melanogaster, etc.). He postulated for the first time that they were the site of intelligence (575). Note: This major discovery will eventually prove to be almost accurate, as these structures are now considered the place where memory and many other behaviors are formed and processed in invertebrates (1812).

 

Robert Remak (PL-DE) described the role of different embryonic germ layers for organogenesis and reduced von Baer’s four germ layers to three and named them: ectoderm (outer skin), mesoderm (middle skin), and endoderm (inner skin) (1595).

 

Moritz Hoffa (DE) and Karl Friedrich Wilhelm Ludwig (DE) described strange unregulated actions of the ventricles (later called ventricular fibrillation) during experiments with strong electrical currents across the hearts of dogs and cats. They demonstrated that a single electrical pulse could induce fibrillation (903).

 

Ernst Heinrich Weber (DE) described the role of elasticity and resistance in the vascular system as a determinant of the distribution and rate of flow of blood throughout the body (2099). In 1827, he had described the effect of the elasticity of blood vessels in transforming the pulsatile movement of the blood in the aorta into a continuous flow of blood in arterioles and capillaries. He demonstrated that the pulse wave traveled at 9.24 meters per second and arrived in the feet a fraction of a second after it reached the jaw.

 

Marshall Hall (GB) coined the phrase spinal shock (825).

 

Daniel Drake (US) wrote, A Systematic Treatise, Historical, Etiological and Practical, on the Principal Diseases of the Interior Valley of North America, as they Appear in the Caucasian, African, Indian, and Esquimaux Varieties of its Population, one of the unique medical treatises of the 19th century (552). Drake is considered one of the greatest American teachers of medicine.

 

Lamuel Shattuck (US), Nathaniel Banks, Jr. (US), and Jehiel Abbott (US) presented their Report of the Sanitary Commission of Massachussetts: Report of a General Plan for the Promotion of Public and Personal Health. The report, based on a sanitary survey of Boston in 1845, contains such far-reaching recommendations as: We recommend that provision be made for obtaining observations on the atmospheric phenomena, on a systematic and uniform plan at different stations within the Commonwealth. We recommend that measures be taken to prevent, as far as practicable, the smoke nuisance. We recommend that, in laying out new towns and villages and in extending those already laid out, ample provision be made for a supply, in purity and abundance, of light, air, and water; for drainage and sewerage, for paving, and for cleanliness.

The report also contains far-reaching recommendations relative to housing, schools, occupational health, and adulterated food and drugs, and recommended that persons be specially educated in sanitary science (1737). In 1948, Charles-Edward Amory Winslow (US) called the Shattuck Report, as it is popularly called, “Perhaps the most significant single document in the history of public health… I know of no single document in the history of that science quite so remarkable in its clarity and completeness and in its vision of the future.”

 

Pierre Paul Broca (FR) described the venous spread of cancer independently of Carl von Rokitansky (CZ-AT) (286; 2056; 2058).

 

William MacIntyre (GB) was the first to describe multiple myeloma (1172).

 

William Detmold (US) opened the lateral sinus of the brain for abscess (521).

 

Alcide Dessalines d’Orbigny (FR) is considered the founder of the science of micropaleobotany. His most important work was the founding of the science of stratigraphical paleontology based on observations of exposed fossil-bearing strata in the Paraná Basin of South America between 1826 and 1834. He studied small marine fossils, pollen, grain and spores found in sedimentary rocks for dating stages. Like his mentor Cuvier, he found that some fossils occurred only in certain layers of a geological formation. He used these fossils to subdivide what we today call the Jurassic into twenty-seven stratigraphic stages, each with its particular fossils (467; 468).

 

An extensive epidemic of dengue fever began in Charleston, SC, and then spread to Savannah, Augusta, New Orleans, Mobile, Galveston, and other southern coastal cities (1030).

 

1851

"It was to Hofmeister, working as a young man, an amateur and enthusiast, in the early morning hours of summer months, before business, at Leipzig in the years before 1851, that the vision first appeared of a common type of Life-Cycle, running through Mosses and Ferns to Gymnosperms and Flowering Plants, linking the whole series in one scheme of reproduction and life-history." Arthur Harry Church (1167). See, Hofmeister below.

 

Alexander William Williamson (GB) was the first person to formulate the concept of chemical reactions reaching an equilibrium state; introducing the phrase dynamic equilibrium. He was also the first person to clearly demonstrate that a specific intermediate compound formed in a catalyzed reaction. This was the formation of ethyl sulfate when sulfuric acid catalyzed the conversion of ethyl alcohol to ethyl ether (2139).

 

Francois Verdeil (FR) suggested a relationship between chlorophyll and heme upon chemical conversion of chlorophyll to a red pigment (1915).

 

Peter Ludwig Panum (DK) found that protein separated from serum and egg white on dilution and the addition of acid. The blood serum gave a precipitate, which would redissolve in water while the egg albumin gave no precipitate. This was one of the first clues that proteins may exist in various chemical types (1407; 1408).

 

Louis René Tulasne (FR), Charles Tulasne (FR) and Heinrich Anton de Bary (DE) described the life history of certain fungi and developed the doctrine of ‘alternation of generations’. They used the terms pleomorphism or heteromorphism to express the observed fact that one and the same fungus appeared in several forms, not only as regards the vegetative but also the fructifying organs, and it was soon shown that this is a widespread type of development, especially among the fungi which cause disease in cereals (490; 492; 494; 1885).

 

Wilhelm Friedrich Benedikt Hofmeister (DE) authored Vergleichende Untersuchungen [Comparative Researches], which represented the beginning of modern work with the Bryophyta (liverworts and mosses) and Pteridophyta (ferns, horsetails, and club-mosses). Here for the first time was a general account of the life histories and reproductive structures of the main types of Bryophyta and Pteridophyta together with some reference to the gymnosperms. Descriptions of prothalli, sex organs, developmental stages in many ferns, and alternation of sexual and asexual generations in plants was here described for the first time. Hofmeister explained how seeds are formed: the megaspore is not released from the megasporangium but germinates there. The pollen grains correspond to the spores of vascular cryptogams (907).

 

Theodor Hartig (DE) furnished the first exact description of the descending sap flow in the 1851 edition of his father’s book, Lehrbuch für Forster und die es Werden Wollen (835).

 

Theodor Hartig (DE) discovered and described sieve tubes in plants (836).

 

Edward Desor (CH-US) published observations on development of a planktotrophic starfish (519).

 

Carl Vogt (DE) recognized the leeches as annelids, placed the nematodes, gordiaceans, acanthocephalans, and gregarines under the class Nematelmia and the true flatworms plus the nemertines under the class Platyelmia (1944).

Karl Gegenbaur (DE) altered Vogt’s names to Platyelminthes and Nemathelminthes. His Platyelminthes contained three groups: Turbellaria (including nemertines), Trematoda, and Cestoda (742).

Ernst Ehlers (DE) put the nemertines in their own group, the Nemertina (613).

Charles Sedgwick Minot (US) then removed the nemertines and placed them in their own phylum (1252).

Libbie Henrietta Hyman (US) emphasized that the etymologically correct form Platyhelminthes (Gk. platys, flat, helminthes, worms) should be used (958).

 

Heinrich Müller (DE) discovered what he characterized as a red pigment in the retina (rhodopsin, or visual purple). He thought it likely to be hemoglobin (1295).

Franz Christian Boll (DE), Wilhelm Friedrich Kühne (DE), and Carl Anton Ewald (DE) independently discovered the visual pigment (rhodopsin) and described the effect of light on this visual pigment found in rod cells of the eye. They showed that light bleaches the visual pigment first to a visual yellow then to a visual white. Kühne solubilized visual pigment (rhodopsin) and suggested that it is a protein (193-195; 929; 1046; 1047; 1050). Boll called the pigment sehrot (visual red). Ewald and Kühne coined the term sehpurpur (visual purple) and gave the pigment its present name, rhodopsin (634; 1049).

Selig Hecht (PL-US) and Edward G. Pickels (US) formally identified visual purple (rhodopsin) as a protein (857).

 

Carl Ferdinand Arlt (AT) presented the proof that shortsightedness is normally a consequence of an elongation of the sagittal axis of the eye (46).

 

Ernst Reissner (LV) discovered the membrane in the cochlea that now bears his name (1588). This discovery allowed the cochlea to be divided into three scalae (media, tympani, and vestibularis).

Alfonso Giocomo Corti (IT) described the cochlear receptor organ in the inner ear (organ of Corti) and in the process discovered the hair cells, the rods (arches) of Corti, and the tectorial membrane (Corti’s membrane) and discussed methods for fixing and staining mammalian epithelial cells of the cochlea. He used chromic acid, ether, acetic acid and alcohol as fixatives with a water/alcohol/carmine mixture as his stain (435).

Gustaf Magnus Retzius (SE) observed the nerve ending on the hair cells (1602).

Rafael Lorente de Nó (ES) showed that each inner hair cell is innervated by one or two nerve fibers and that each nerve fiber branches to innervate only a few hair cells (1140).

 

Hermann Ludwig Ferdinand von Helmholtz (DE) pioneered the scientific study of perception. In 1850, he invented an ophthalmoscope to see within the eye, an ophthalmometer to measure the curvature of the eye, and in 1852 the phakoscope for studying the changes in lens during accommodation, revived and expanded Young’s three-color vision theory (there are three fundamental color sensations—red, green, and blue), and advanced the theory that the ear detects differences in pitch through the action of the cochlea in the inner ear (1987; 1989; 1990). He discovered that light which impinges directly on the optic nerve does not give rise to any sensation, but that it must fall on a nerve ending in the retina before it can be perceived.

 

Thomas Henry Huxley (GB) confirmed that the life cycle of Salpa goes through an alternation of solitary and chain-like colonial generations. He discovered that the solitary form is the product of the sexual generation and the colonial form results from budding (asexual). He determined that the tail of Appendicularia is a retained larval feature lost by most adult ascidians. He named the phylum Coelenterata (870; 942; 944-946; 948; 951; 952; 954; 956).

 

Thomas Henry Huxley (GB) popularized the phrase describing protoplasm as the physical basis of life. See, Hugo von Mohl, 1846. Huxley was the greatest champion of Darwin’s theory of evolution (957).

 

Josef von Gerlach (DE) was the first to show that human skin uses oxygen from ambient air (1976).

 

Friedrich Theodor von Frerich (DE) theorized that uremic intoxication results from the action of a plasma enzyme on the increased amount of urea thus leading to the release of ammonium carbonate (1975).

 

Frans Cornelis Donders (NL) introduced the use of cylindrical and prismatic glasses in impaired vision. He investigated the differentiation of presbyopia and hyperopia, along with the movements of the eye and the accommodation of the pupil. He devised sets of letters of different sizes for testing a patient’s visual acuity.

In his book, On the Anomalies of Accommodation and Refraction of the Eye, Donders separated the errors of refraction (the bending of light) from those of accommodation (the change in the shape of the lens of the eye to maintain focus). He described the errors in refraction as constant, resulting from light being focused at a constant point either behind or in front of the retina, depending on the shape of the eye. Errors in accommodation, he discovered, result from defects in the eye’s focusing machinery, such as weak ciliary muscles (the muscles that change the shape of the lens) or a hardening of the lenses. These achievements labeled Donders as one of the first scientists who explored the physical field of the eye (534-537).

 

Karl Friedrich Wilhelm Ludwig (DE), Emil Becher (DE), and Conrad Rahn (DE) discovered innervation of the submaxillary gland and showed that its secretion is not dependent on blood pressure but rather the gland cells respond like muscle cells to special nerves (94; 1072; 1540). In 1856, Ludwig found that stimulation of the lingual-chorda tympani would cause secretion by the submaxillary gland (1155).

 

Pierre Paul Broca (FR) described muscular dystrophy as a primary affliction of muscle (287).

 

Thomas Addison (GB) and William Whitey Gall (GB) described hypercholesterolemia (FH) (14).

Thomas Addison (GB) described a case of xanthoma diabeticorum as follows: "an eruption somewhat suddenly appeared on the arms…. It consisted of scattered tubercles of various sizes, some being as large as a small pea…. When incised with a lancet they were found to consist of firm tissue…. They were of a yellowish colour, mottled with a deepish rose-tint…. [At the beginning of March] many of the tubercles began to subside." (2135)

C. Hilton Fagge (GB) described a case of xanthomastosis with cardiovascular symptoms (636).

Julius Ehrmann (DE) described xanthomas and lipomas (621).

Georg Lehzen (DE), and Karl Knauss (DE) reported the case of an 11-year-old girl presenting with the steadily growing xanthomas, accompanied by cardiovascular symptoms. She died suddenly, and the post-mortem study revealed exanthomatous deposits in the aorta, with the narrowing isthmus, as in other large arteries. Later, her sister manifested similar skin changes, highlighting this case to be an early report on homozygous familial hypercholesterolemia (1094).

 

Bernard Nièpce (FR) was the first to report pituitary hyperplasia following primary hypothyroidism. He observed the enlargement of sella turcica (bony structure around the pituitary gland) in cretins with hypothyroidism (1345).

 

Ernst Gustav Benjamin von Bergmann (DE), beginning around 1851, popularized aseptic surgery as an outgrowth of the growing appreciation of the importance of infected persons as the primary source of sepsis. This was an important transition from the clumsy antiseptic technique to the more practical aseptic technique (1955-1957). Note: Bergmann’s assistant, Kurt Schimmelbusch (DE) helped to make the term ‘asepsis’ a widespread and powerful means of branding the new approach. Because of this he is often counted among the inventors of asepsis.

Hermann Kümmell (DE) crusaded in Germany for the routine of scrubbing up for surgery (1051).

Paul Fürbringer (DE) came up with a standardized procedure in which he first washed his hands with soap, then alcohol, and finally an antiseptic substance. Fürbringer’s laboratory-based procedure was quickly adopted as the “gold standard” of hand disinfection in surgery (720; 721).

William Stewart Halsted (US) had rubber gloves specially made for operating. This article makes the first mention of their use in an operating room (827).

Jan Mikulicz-Radecki; Johannes von Mikulicz-Radecki (PL-AT) described the use of sterilized linen gloves and a mouth covering of gauze during surgery (2046).

 

Jean-Pierre Falret (FR) described a condition he called la folie circulaire (circular insanity), of which a patient would experience cycles of manic excitement and cycles of depression. Falret's description is the earliest documented diagnosis of what today is known as a bipolar affective disorder (638; 639).

Jules-Gabriel-François Baillarger (FR) described a psychiatric disorder involving both manic and depressive episodes in the same individual, a condition that he referred to as folie à double forme (dual-form insanity) (69).

 

The journal Wiener Medicinische Wochenschrift was founded.

 

1852

"Fear not! Life still leaves human effort scope.

But, since life teems with ill,

Nurse no extravagant hope". Mathew Arnold (48)

 

"By felling the trees which cover the tops and sides of mountains, men in all climates seem to bring upon future generations two calamities at once; want of fuel and a scarcity of water." Friedrich Wilhelm Heinrich Alexander von Humboldt (DE) (1996).

 

Edward Frankland (GB) was the first to study and synthesize organometallic molecules. During his reading of a paper to the Royal Society on organic metallic compounds he made the empirical observation that elements possess fixed combining powers, or "only room, so to speak, for attachment of a fixed and definite number of the atoms of other elements." (705) This is the theory of valence, although the term valence or valency began to be used only after 1865.

Alexander Crum-Brown (GB) developed a system of graphic formulation of compounds which is essentially identical with that used today. His formulae were the first to show clearly both the valency and the linking of atoms in organic compounds (451).

Carl Hermann Wichelhaus (DE) introduced the term valenz (valence) to denote what had been termed saturating capacity or atomicity (2122).

Richard Wilhelm Heinrich Abegg (PL-DE) proposed that a chemical reaction became the transfer of electrons and that chemical bonds became the attraction between opposite electric charges. (It turned out that he was correct for ionic bonding but not for covalent bonding.) He suggested that the outer electron shell governs the chemical properties of an atom. Noting that the inert gases (neon, argon, krypton, xenon, and radon) all have eight electrons and are especially stable, he proposed what become known as Abegg's rule: the difference of the maximum positive and negative valence of an element tends to be eight (5).

Walther Kossel (DE) and Gilbert Newton Lewis (US) independently proposed the electronic theory of valency and brought the new atomic physics to bear on chemical problems. This theory led organic chemists and biologists to consider reactions in their domain as involving electronic mechanisms. Lewis developed a theory that focused on the significance of valence electrons (electrons in the outer shell) in chemical reactions and in bonding. Lewis’s theory of the cubical atom layed the groundwork for Irving Langmuir’s (US) octet rule, in which atoms form bonds by losing, gaining, or sharing enough electrons to have the same number of valence electrons (eight) as the nearest noble gas in the Periodic Table. The bond formed is ionic or covalent depending on whether the electrons are transferred or shared between atoms (1036; 1069; 1070; 1115; 1402).

Erich Armand Arthur Joseph Hückle (DE) proposed the molecular orbital theory (930-935).

Rudolph Pariser (US), Robert Ghormley Parr (US), and John Anthony Pople (GB) presented the Pariser-Parr-Pople method of molecular orbital computation (1414-1416; 1491).

Roald Hoffmann (PL-US) developed the extended Hückel method, a molecular orbital scheme which allowed the calculation of the approximate sigma- and pie-electronic structure of molecules, and which gave reasonable predictions of molecular conformations and simple potential surfaces (904).

 

Raffaelle Piria (IT) described populin (benzoyl salicin) from the bark and leaves of the poplar tree (1471).

 

Ludwig T. Stawiarski Teichmann (PL) isolated an iron-containing molecule from the blood of various animal types. He named it haemin (1825). Today we know this was hemoglobin and that he demonstrated it contains iron.

 

Charles Naudin (FR) compared the origin of species in nature with that of varieties under cultivation. "We do not believe that, when nature created species, it acted in a way any different from the way we act to create varieties; in plain words, we have transposed nature’s processes into our practice. What is this process? It is to attune each member with the whole by assigning to it the function it must carry out in the general organism of nature, a function that is its raison d’etre." (1329)

 

 Augustus Volnay Waller (FR-GB) confirmed Fontana’s discovery that cut nerve cells could regenerate by demonstrating that regeneration begins proximally to the point of the cut and not distally to it then concluded that the cell body was necessary for cell survival and regeneration (2082). Franz Nissl (DE) would experimentally support this finding (1350).

Waller established that all nerve fibers are filiform cellular processes connected with cell bodies (ganglionic corpuscles) and that the fibers degenerate if separated from their cell bodies (2083).

He expressed his concept of the nutritive influence of nerve cell bodies on the whole fiber and concluded that the nutritive cell bodies for the sensory fibers are in the spinal ganglia, while, for the motor fibers, the nutritive cell bodies are in the anterior horns of the spinal cord (2084).

He obtained experimental evidence for a perennial intraneuronal equilibrium between anabolism and catabolism (2085).

 

Hermann Friedrich Stannius (DE) performed experiments on animals, which indicated that the heartbeat can be inhibited by the vagus nerve and that the heart contains a pacemaker. He tied ligatures between the sinus venosus and the atrium, and between the atrium and the ventricles of a frog heart and demonstrated that the sinus is the pacemaker of the heart, yet the atria and ventricles are capable of independent, spontaneous contractions (1787; 1788). See, Ernst Heinrich Weber and Eduard Friedrich Weber, 1845.

Ludwig Traube (DE) also found that if the vagus nerve is cut, the speed of the pacemaker—consequently heart contractions— cannot be slowed (1871; 1872; 1874).

 

Ludwig Traube (DE), in 1852, produced the first graphic presentation of a fever course with simultaneous recording of pulse and respiratory frequency (1874).

 

Friedrich Heinrich Bidder (DE) and Carl Schmidt (DE) showed the importance of bile in emulsifying fat in the intestine (149).

 

Karl Vierordt (DE) invented the first quantitative method of counting erythrocytes. He found that man averages 5,714,400 erythrocytes per cubic centimeter (1919-1921).

Hermann Welcker (DE), in 1853, counted white blood cells in man and reported 12,133/ cubic millimeter (382).

Hermann Welcker (DE), in 1854, counted the cells in a patient with chlorosis (an old word for what is probably our modern iron-deficiency anemia) and found that an anemic patient had significantly fewer erythrocytes than a normal person (1898).

Hermann Welcker (DE), c. 1854, made hemoglobin estimates (382).

Antony Cramer (NL) introduced dilution and counting chambers for blood cells (448).

Magnus Gustaf Blix (SE), in 1885, offered an alternative to counting erythrocytes. He suggested the use of centrifugal force to pack the red cells together and to estimate their number by measuring their volume (1626).

Pierre Carl-Joseph Potain (FR), in 1867, invented the blood-diluting pipette (382).

Georges Hayem (FR) reported the first accurate platelet counts. The platelet numbers he reported do not differ significantly from those reported as normal today (854).

William Richard Gowers (GB) invented the modern hemocytometer (778).

William Richard Gowers (GB) developed a method for determining the quantity of hemoglobin in a blood sample (780).

Sven Gustaf Hedin (SE) developed a machine for measuring packed erythrocytes—the "hämatokrit"(hematocryte) (858).

Leon L. Blum (US) laid down certain principles of turbidometry, which led to the use of light transmission as a method of counting blood cells (187; 188).

 

Rudolf Albert von Kölliker (CH) wrote, Handbuch der Gewebelehre des Menschen, fur Aerzte und Studirende, the first textbook of histology. Many of his descriptions have never needed correction (2006). Here he found that more than one nucleus occurs in the giant cells (polykaryocytes) of bone marrow and in certain nerve cells.

 

Rudolph Wagner (DE) and Georg Meissner (DE) discovered the encapsulated receptors for touch located in the connective tissue papillae of the skin. They conceived the stimulation of these tactile corpuscles as pressure changes in the skin, which in turn triggered neural responses (1235; 2070). In their honor these touch receptors are called Wagner’s corpuscles and Meissner’s corpuscles.

 

George Meissner (DE) gave the first description of Tinea ungium with detection of fungi in the nail material as the etiologic cause (1236).

Rudolf Ludwig Karl Virchow (DE) named this disease onychomycosis (1929).

 

Rudolph Ludwig Karl Virchow (DE) became convinced that connective tissue cells secrete fibrillar intercellular substance (1927).

 

Robert Remak (PL-DE) argued that proliferation of cells to form tissues is accomplished by cell division and that pathological new growths in animals represent cells arising from previously existing cells (1596; 1598; 1599).

 

Clemens Heinrich Lambert von Babo (DE) demonstrated the rapid separation of blood-corpuscles from the serum by centrifugation (1947).

 

Maximilian Perty (DE) created Ciliata for the ciliated forms of protozoa (1452).

 

Thomas Henry Huxley (GB) grouped all the free-swimming tunicates he had studied into the same group, Ascidiacea (sea squirts), based upon their typical structure (943; 953).

 

Rudolf Albert von Kölliker (CH) described how motor nerves originate from the neurons in the nucleus glossopharyngei (anterior horn) of the spinal cord (2006).

 

Karl Friedrich Wilhelm Ludwig (DE) and Adolf Beutner (DE), in 1850, were the first to measure blood pressure in the pulmonary artery. They used cats, rabbits, and dogs in which they opened the left pleural cavity then inserted a manometer directly into the pulmonary artery (145).

Karl Friedrich Wilhelm Ludwig (DE) was the first to carry out in hemodynamics the differentiation between measurements of lateral versus retrograde pressure. Ref

 

Charles-Édouard Brown-Séquard (FR) foreshadowed Claude Bernard's discovery of the vasomotor system by showing in the rabbit that stimulation of the cervical sympathetic causes blanching of the ear (305-307).

 

Auguste Nélaton (FR) first described a disease characterized by sensory disorders of the lower extremities, leading to perforating ulceration of the feet and destruction of the underlying bones (1332). This disease would be called Nélaton's syndrome.

Eric Perrin Hicks (GB) reported a family as having hereditary perforating ulcer of the foot. Derek Ernest Denny-Brown (NZ-GB-US) reported on this same family and called the disease hereditary sensory radicular neuropathy. It is today called hereditary sensory neuropathy type 1 (HSN1) and recognized as the most common dominantly inherited degenerative disorder of sensory neurons (517; 896).

 

Karl Theodor Ernst von Siebold (DE) first identified Hymenolepis nana syn. Rodentolepis nana, the dwarf tapeworm, as a human parasite in 1852 (1175).

Charles Wardell Stiles (US) identified an identical parasite with a rodent host and named it Hymenolepis fraterna syn. Rodentolepis fraterna (1802).

Hymenolepis is a genus of cyclophyllid tapeworms responsible for hymenolepiasis. They are parasites of humans and other mammals.

 

Theodor Maximillian Bilharz (DE) described heterophyiasis, infection with the intestinal fluke Heterophyes heterophyes, which is acquired by eating infected raw or undercooked fish from freshwater or brackish water (158).

 

Theodor Maximillian Bilharz (DE) described hymenolepiasis as an infestation by one of two species of tapeworm: Hymenolepis nana or H. diminuta (158). Alternative names are dwarf tapeworm infection and rat tapeworm infection. The disease is a type of helminthiasis which is classified as a neglected tropical disease.

 

Edward Meryon (GB) was the first to give a definite description of what is called Duchenne-Griesinger disease. He reported on four affected brothers whom he had studied for several years. He took pains to conduct postmortem microscopic examinations of muscle tissue from the brothers observing the utter destruction of muscle fibers, as well as, the breakdown of the surrounding muscle sheath, a feature that Duchenne would fail to note (1241). Also known as: Duchenne de Boulogne muscular dystrophy, Duchenne's muscular dystrophy (DMD), Duchenne's myodystrophy, Duchenne's pseudohypertrophic muscular dystrophy, Duchenne's syndrome, and Griesinger's disease or syndrome. DMD was the first well-characterized muscular dystrophy.

Wilhelm Griesinger (DE) and Guillaume Benjamin Amand Duchenne (FR) described a condition—Duchenne-Griesinger disease— characterized by weakness and pseudohypertrophy of the affected muscles. The disease begins in childhood, usually between 2 and 6 years, is progressive, and affects the shoulder and pelvic girdle muscles (564; 800). In the 1868 article he mentions his use of a biopsy procedure to obtain tissue from a living patient for microscopic examination. Duchenne constructed a biopsy needle (Duchenne’s trocar), which made possible percutaneous muscle biopsies without anesthesia.

William Richard Gowers (GB) recognized that only boys are affected, and that the disease is transmitted through healthy mothers to their sons (779). See, Broca, 1851.

 

Carl von Rokitansky (CZ-AT) wrote his excellent monograph on diseases of the arteries in which he described, but did not name, polyarteritis nodosa in a 23-year-old man with a 5-day history of fever and diarrhea (2057).

Carl Philipp Adolf Konrad Kussmaul (DE) and Rudolf Maier (DE) were the first to use the phrase periarteritis nodosa and provide its classic description, the prototype of systemic necrotizing vasculitis (1054).

Pearl M. Zeek (US) made the first attempt to classify vasculitis by subdividing it into five categories: hypersensitivity angiitis, allergic granulomatous angiitis, rheumatic arteritis, periarteritis nodosa, and temporal arteritis. This first classification of vasculitis did not include granulomatosis with polyangiitis (GPA – formerly Wegener’s granulomatosis) or Takayasu arteritis (2178).

J. Davson (GB), J. Ball (GB), and Robert Platt (GB) described a microscopic form of periarteritis nodosa that was later recognized as being closely related to GPA and to eosinophilic granulomatosis with polyangiitis (EGPA – formerly Churg-Strauss syndrome) (487).

Note: The most widely accepted way of classifying systemic vasculitis includes the predominant size of affected vessels, the association with an etiological agent (i.e., primary or secondary vasculitis) and the presence of antineutrophil cytoplasmic antibodies (ANCA).

 

William Senhouse Kirkes (GB) discussed embolia as follows: "The effects produced and the organs affected will be…determined by the side of the heart from which the fibrinous masses have been detached; for if the right valves have furnished the source of the fibrine, the lungs will bear the brunt of the secondary mischief, displaying it in coagula in the pulmonary arteries…but if…the left valves are affected, the mischief…may fall on any systemic part, but especially…the brain, spleen, and kidneys." (1012)

Rudolph Ludwig Karl Virchow (DE) demonstrated that masses in the blood vessels resulted from thrombosis (a term he coined) and that portions of a thrombus could become detached to form an embolus (also his term). An embolus set free in the circulation might eventually be trapped in a narrower vessel and lead to a serious lesion in the neighboring parts. He also explained the genesis of thrombophlebitis and was one of the first to recognize lung-and cerebral embolisms (1936; 1938; 1939).

 

Nikolai Ivanovich Pirogov; Nicholas Ivanovitch Pirogoff (RU) produced the first significant publication on the technique of frozen sections. He thought he had invented the technique, not realizing that Pieter de Riemer (NL) had priority. See, Pieter de Riemer, 1818.

This is also a major work on topographical anatomy and laid a firm foundation for that field as a special area of science having great practical significance for surgery (1473).

 

James Marion Sims (US), in 1845, surgically repaired vesicovaginal fistula used lateral positioning of the patient, invention of a special curved speculum, and use of silver sutures and a silver catheter to operate successfully for vesicovaginal fistula, a frequent and distressing complication of childbirth seen particularly among the poor (1761).

 

John Simon (GB) performed the first uretero-intestinal anastomosis, an operation for directing the orifices of the ureters into the rectum (1746).

 

1853

"Never, in any circumstances, is an optically active compound produced by a non-living body, while almost all the substances elaborated by nature in vegetable organisms are asymmetrical, in the manner of tartaric acid." Louis Pasteur (1425)

 

Charles Frédéric Gerhardt (FR) coined the word glyceride in reference to simple compounds forming fats and oils (746).

 

Patrick J. Duffy (GB) found that crystallized tristearin, a triacylglycerol, has three melting points (570).

 

Johann Justus von Liebig (DE) isolated and named kynurenic (Gk. kynos, dog + ouron, urine) acid from dog’s urine (2040).

 

Ludwig Karol Teichmann- Stawiarski (PL) showed that hemin could be crystallized from dried blood using sodium chloride and hot glacial acetic acid (1824). By 1857 this had been introduced into forensic chemistry as a test for blood.

 

Johannes Petrus Müller (DE) and Ferdinand Julius Cohn (DE) independently separated out the flagellated protozoa into their own group, Flagellata (394).

Karl Moritz Diesing (AT) suggested the name Mastigophora to replace Flagellata (530).

 

Gustave Adolphe Thuret (FR) and Joseph Decaisne (FR) showed that in Fucus, a brown marine alga, the eggs had to be activated by sperms before they could germinate, i.e., microgonidia (or spermatozoids) attach themselves to the macrogonidia (or egg cell). This represents the discovery of sexual reproduction among the cryptogams (1850-1852).

Ferdinand Julius Cohn (DE) described the life history of the alga Sphaeroplea annulina (395).

Nathanael Pringsheim (DE) confirmed sexuality in the alga and demonstrated alternation of generations using Vaucheria, Oedogonium, Coleochaete, and Pandorina (1495-1507). Pringsheim is honored with the genus Pringsheimiella.

Ladislav Josef Celakovsky (CZ) proposed his "antithetic theory", according to which a regular alternation of generations occurs in the higher cryptograms only through the interpolation of a new, nonhomologous generation (the sporophyte) arising from the division and progressive sterilization of the zygote of a primitive, sexually reproducing plant (353; 354).

 

Louis René Tulasne (FR) and Charles Tulasne (FR) demonstrated that a species of fungal rust produces more than one type of spore and that some rusts had three spore forms, which we now call urediospores, teliospores, and sporidia (basidiospores) (1886-1888).

 

Philipp Bruch (DE), Wilhelm Philip Schimper (DE), and Theodor Gümbel (DE) authored Bryologia Europea, one of the most important bryological floras ever published. It set the standard in illustration and description for all subsequent works (312).

 

August David Krohn (RU-DE) was the first to describe double-shelled echinospira larvae (1041).

 

John Graham Dalyell (GB) performed th first embryological study of flatworms (471).

 

John Snow (GB) presented the intellectual framework on which his discoveries of the decade or two later were to be built. His most notable application of this framework was his work on the epidemiology of cholera (1771).

 

Thomas Henry Huxley (GB), based on comparative anatomy and comparative embryology (largely from Karl Ernst von Baer), concluded that the Cephalopoda, Gastropoda, and Lamellibranchiata are all members of a larger common group (947; 955).

 

Edme Félix Alfred Vulpian (FR) reported on the origins of cranial nerves III to X (2064).

 

Ernst Adolf Coccius (DE) was the first to describe a break in the retina of the eye and subsequently made the association with retinal detachment (387).

Jules Gonin (CH) was the first to observe the importance of retinal tears in the development of retinal detachments (765; 766).

Jules Gonin (CH) pioneered the procedure of ignipuncture and thermocautery, the first successful surgery for the treatment of retinal detachments (767; 768).

 

Karl Vierordt (DE) invented a non-invasive sphygmograph for recording the pulse (1923). It was presented at a meeting in 1853.

Karl Vierordt (DE) established the modern method of estimating blood pressure by adding weights to a sphygmograph (sphygmos is Greek for pulse) (1922; 1923).

Samuel Siegfried Karl von Basch (CZ), in 1881, invented the sphygmomanometer. His device consisted of a water-filled bag connected to a manometer. The manometer was used to determine the pressure required to obliterate the arterial pulse (1954).

 

Augustus Volnay Waller (FR-GB) discovered the vasoconstrictor action of the sympathetic nervous system (2087).

 

Smallpox (red plague) was introduced to Hawaii by a ship arriving from San Francisco. At least 2500, and possibly as many as 5,000 people died (1030).

 

1854-1860

Sweden and Germany experienced an epidemic of cerebrospinal meningitis.

 

1854

"Dans les champs de l’observation, le hasard ne favorise que les esprits préparés [In the fields of observation, chance favors only the prepared minds]." Louis Pasteur {Vallery-Radot, 1909 #3757}

 

Pierre Eugène Marcellin Berthelot (FR) described glycerol as a tribasic alcohol and successfully combined it with fatty acids to yield mono- di- and triacylglycerols (138).

Richard G. Jensen (US), Joseph Sampugna (US), R.L. Pereira (US), Ramesh C. Chandan (US), and Khem M. Shahani (US) described for the first time the synthesis of mixed acid triacylglycerols (975).

 

Rudolf Ludwig Carl Virchow (DE) was the first to use the term amyloid to name the tissue condition other pathologists had called lardaceous or cholesterin disease (Speck- oder Cholesterin-krankheit), wax-spleen (Wachs-milz), sago spleen, and lardaceous spleen (1228; 1930; 1931).

Alan S. Cohen (US) and Evan Calkins (US) discovered that amyloid has a precise fibrous ultra- structure (391).

Alan S. Cohen (US) layed out the basic pathobiologic, immunologic, and biochemical factors involved in the genesis of amyloidosis, as well as, the clinical behavior of the disease. This has led to a clearer understanding of its manifestations. The description of the various clinical types including an analysis of the increasing numbers of heredofamilial amyloidoses has led to a greater awareness of their presence and greater interest in their diagnosis (388-390).

 

Pierre Eugène Marcellin Berthelot (FR) was among the first to accomplish in vitro synthesis of fats, formic acid, methyl alcohol, ethyl alcohol, methane, benzene, and acetylene. He was the first to synthesize organic substances, which did not occur in nature (138; 139). He coined partition coefficient, explosion wave, saccharose, and acetylene. He was the first to use the word synthesis to mean the production of organic compounds from their elements.

 

Hermann Welcker (DE) was the first to determine the total blood volume and the size, number, surface area, and volume of the normal blood cells (2110-2112).

 

John Bennett Lawes (GB) and Joseph Henry Gilbert (GB) used animal feeding experiments to clearly demonstrate that proteins from cereal and legume seeds differed in nutritive value (1080).

 

The first recorded recommendation of sodium chloride as an herbicide occurred in Germany (1858).

 

The first documented insecticide in the United States was a sulfur-tobacco dip employed to control sheep scab in 1854. The sheep mite, Psoroptes communis causes sheep scab (1733). The inventor is unknown. The Department of Agriculture was not established until 1862.

 

Wilhelm Reuling (DE) used logwood (hematoxylin) paper indicator to show that ammonia is not excreted through the lungs during breathing (1604).

 

Nathanael Pringsheim (DE) noticed that solutions of salts, acids, and sugar caused the zellinhalt (cell contents) to collapse inwards away from the cell wall (1494).

Karl Wilhelm Nägeli (CH) discovered that many plant cells respond to a hypertonic solution by retracting their contents. He attributed this to a semi-permeable cell membrane allowing water to leave the cytoplasm (1324).

Hugo Marie de Vries (NL) named and defined plasmolysis as the detachment of the living protoplasm from the cell wall through the action of aqueous solutions. He realized that the membrane responsible for this osmotic phenomenon is at the boundary of the vacuole, and since it was involved in the maintenance of turgor, he named it the tonoplast (510; 511).

 

Theodor Hartig (DE), while observing sieve plates, in 1837, was probably the first to see plasmodesmata or connective strands that sometimes connect cells (840).

Eduard Adolf Strasburger (PL-DE) introduced the name of plasmodesmen (singular plasmodesma) (1811), The Greek plural, plasmodesmata, became the preferred term (1811).

 

Heinrich Georg Schroeder (DE) and Theodor von Dusch (DE) heated an infusion and the air over it in a flask. The flask was connected to both a sterile tube containing sterile cotton wool and a vacuum tank called a gasometer where the vacuum was produced by lowering the water level. Once the infusion and all associated apparatus cooled the vacuum tank was used to draw fresh air through the cotton wool and into the infusion flask. Meat infusions and malt broths treated this way remained free of putrefaction for approximately one month. If the cotton wool was not in place a similarly treated infusion became putrid by the second week. These two scientists were the first to use cotton plugs to prolong the sterility of culture flasks and tubes. They were not successful, however, in preventing contamination of milk or of meat, without the addition of water, even when the air entering the flask had been filtered through cotton wool (1701-1703).

 

Filippo Pacini (IT), during the cholera epidemics in Florence 1854-1855, microscopically examined the blood and feces of those afflicted with the disease as well as the changes of the intestinal mucosa of cholera corpses. This work was done with the support of his assistant, Francesco Magni (IT). These investigations proved the presence of millions of rod-shaped corpuscles, which he considered to be microbes, and so named them. He stated that cholera is a contagious disease characterized by destruction of the intestinal epithelium, followed by extreme loss of water from the blood (for which condition he later recommended, in 1879, the therapeutic intravenous injection of saline solution). Pacini went on to declare that the intestinal injuries common to the disease were caused by living microorganisms— which he called "vibrions"; he further provided drawings of the vibrions that he had observed microscopically in abundance in the intestine of cholera victims (1396; 1397).

Heinrich Hermann Robert Koch (DE) later isolated the cholera vibrio from cases of cholera in Egypt. Then, while in the Orient as the head of the German Cholera Commission, he discovered the cholera vibrio in the intestinal discharges of cholera patients, thereby providing a ready and successful means of quarantine control of the disease (1021-1024). This microorganism has gone by various names including: Spirillum cholerae asiaticae (Koch), Spirillum cholerae, Vibrio cholerae, the comma bacillus, and Vibrio comma (Bergey). In 1965 the international committee on nomenclature adopted Vibrio cholerae Pacini 1854 as the correct name of the cholera-causing organism.

 

George Newport (GB) and George Viner Ellis (GB) proved that it was the sperm cells, not the fluid, in semen, which fertilized frog eggs. He also found that the point of sperm entry determines the plane of the first cleavage and the axis of the developing embryo (1336).

 

Robert Remak (PL-DE) was the first to use hardening agents to improve definition of histological preparations (1597).

 

Theodor Hartig (DE) reported on staining plant chlorogen granules (chlorophyll) and other parts of the cell using gamboge, carmine, cinnabar, copper sulfate, litmus, and ink (837-839).

 

Rudolf Ludwig Karl Virchow (DE) discovered the neuroglia (1928).

 

John Simon (GB) was a physician and public health reformer of great importance in the 19th century. The eight annual reports that Simon presented to the Corporation of London are the most famous health reports ever written. They led directly to the Sanitary Act of 1866 and the great Public Health Act of 1875. The latter provided a complete sanitary code upon which the present system is still founded. The good public health infrastructure in western countries is his legacy.

 

Maximilianus Carolus Augustus Flinzer (DE) introduced the use of silver stain in his study of the cornea (695).

Bartolomeo Camillo Golgi (IT) discovered the silver nitrate stain for nerve cells (763). See, Golgi, 1873

Louis-Antoine Ranvier (FR), and Santiago Ramón y Cajal (ES) popularized the use of silver stains in neurological histology (1544; 1545).

 

Louis Pierre Gratiolet (FR) deduced that the two sides of the brain control movement of the opposite sides of the body. He decided that the progression on intellect was clear from lower vertebrates to cats to dogs to monkeys and to man (789).

 

William Stokes (IE) wrote, The Diseases of the Heart and the Aorta, which contains a very early account of aortic valve stenosis, paroxysmal tachycardia and a description of Cheyne-Stokes respiration. In 1846 he described bradycardia, Cannon waves, and heart block, since known as Adams-Stokes disease (1805; 1806).

Giovanni Battista Morgagni (IT), Thomas Spens (GB), and Robert Adams (IE) had previously described cases of Adams-Stokes disease; the description by Adams being the most scientific (10; 1267; 1777).

 

Rudolf Peter Heinrich Heidenhain (DE) demonstrated that the vagus nerve functions to regulate heart activity; the automatic activity seemed to him to originate in the ganglia of the heart (860).

 

Nikolai Ivanovich Pirogov; Nicholas Ivanovitch Pirogoff (RU) pioneered the medical use of plaster casts during the Sevastopol campaign of the Crimean War in 1854. He conceived the idea while observing the work of a sculptor (1475; 1476).

 

Nikolai Ivanovich Pirogov; Nicholas Ivanovitch Pirogoff (RU) invented an osteoplastic foot amputation at the ankle, removing a portion of the os calcis. The foot is severed so that part of the heel bone is left in the stump to give added support to the lower ends of the leg bones (1474).

 

Gabriel Colin (FR), to explore the psychology and physiology of the horses' feeding process, experimented with various foods and chemicals (404).

 

Ferdinand Vandeveer Hayden (US) and Fielding Bradford Meek (US), near the confluence of the Missouri and the Judith Rivers, collected unusual teeth later determined by paleontologist Joseph Leidy (US) to be those of the dinosaurs Trachodon, Troodon and Deinodon, making the 1854 expedition by Hayden and Meek the first in North America to uncover dinosaur remains (1096).

 

Aerztliches Intelligenz-Blatt was founded. It became Müncher Medicinsche Wochenschrift in 1886.

 

Archiv für Ophthalmologie was founded; later renamed Albrecht von Graefe’s Archiv für Ophthalmologie, then Graefe's Archive for Clinical and Experimental Ophthalmology.

 

Europe experiences a pandemic of dysentery (the "bloody flux").

 

1855

"Plants, in a state of nature, are always warring with one another, contending for the monopoly of the soil, - the stronger ejecting the weaker, - the more vigorous overgrowing and killing the more delicate. Every modification of climate, every disturbance of the soil, every interference with the existing vegetation of an area, favours some species at the expense of others." John Dalton Hooker (GB) (912)

"For researches [on circulatory changes] the Frog—that arch-martyr to science—affords the most convenient subject." Carl Wedl (2103)

 

Hermann Emil Fischer (DE) isolated valine from casein and determined its structural formula (677). Fischer named it valine from the Latin validus meaning healthy (678).

 

Charles Adolphe Würtz (FR) proposed the correct formula for glycerol (2174).

 

Friedrich Gaedcke (DE) announced that he had isolated the active alkaloid ingredient from coca (Erythroxylum coca) leaves. He named it erythroxyline (cocaine) (722).

Albert Niemann (DE) is also credited with isolating cocaine from coca plant tissue in 1859 and coining the name cocaine (1343; 1344).

 

In 1855, Germany introduced sulfuric acid as a herbicide in cereals and onions (2182).

 

Gottleib Carl Haubner (DE) was the first to conduct digestion trials and to discover that fiber (cellulose) is, in fact, partly digestible (850).

Wilhelm Henneberg (DE) and Friedrich Stohmann (DE), 1860-1864, conducted trials to determine the digestibilities of all the proximate constituents for a variety of feeds. In 1864, they showed, among other things, that in some cases the crude fiber was more digestible than the nitrogen-free extract (NFE), and that the indigestible portion of the NFE was a noncarbohydrate constituent called lignin (881).

Wilhelm Henneberg (DE) and Fridrich Strohmann (DE) studied the fate of cellulose in the food of herbivores and found it to have a high nutritional value. They speculated that microbial action converted cellulose into organic acids, and methane (882).

Hans Pringsheim (CZ-GB) demonstrated that certain cellulose-digesting bacteria liberate simple sugars, and disaccharides (1493).

 

William Senhouse Kirkes (GB) published a study of apoplexy in Bright's disease. He pointed clearly to the role of raised intra-arterial tension in the causation of arterial disease (1013).

Ludwig Traube (DE) discovered that hypertension often contributes to advanced kidney disease as do fluid and electrolyte disturbances (1873).

F.M. von Heymann (DE) described the optic changes of malignant hypertension (1992). Later called hypertensive neuroretinopathy by Fishberg and Oppenheimer in 1930.

Frederick Henry Horatio Akbar Mahomed (GB) was also one of the originators of the concept that high blood pressure could damage the kidneys and blood vessels (1194; 1196).

 

Theodor Hartig (DE) described aleurone grains and discovered the nucleus in aleurone cells and was the first to describe it as the basic component of cells (841; 842).

 

Karl Gotthelf Lehmann (DE) reported that fats taken in during a meal are unaltered in the stomach then passed across the intestinal wall of the duodenum where they appear as fat globules in the lacteals. He was mystified as to how fats crossed the membranes of cells near the lacteals (1093).

 

Richard Ladislaus Heschl (AT) described the transverse gyri in the temporal lobe (Heschl's gyri) (891). This anatomical structure processes incoming auditory information.

 

Richard Liebreich (DE) was the first to describe central retinal vein occlusion (1122).

 

John Russell Reynolds (GB) described the symptomology of tabes dorsalis (locomotor ataxia) (1605).

 

Heinrich Adolf Rinne (DE) developed a hearing test (Rinne test) conducted with a tuning fork that is used to test and compare a patients' hearing via air conduction (normal process) or by way of bone conduction (sound to the inner ear through the mastoid). He reasoned that if a person hears a sound for a longer period of time through bone conduction than through air conduction, a disease is present somewhere in the conduction apparatus (1613).

 

Auguste Louis Jules Millard (FR) and Adolphe-Marie Gubler (FR) described ventral pontine syndrome (Millard–Gubler syndrome) with symptoms that result from the functional loss of several anatomical structures of the pons, including the sixth and seventh cranial nerves and fibers of the corticospinal tract. Paralysis of the abducens (CN VI) leads to diplopia, internal strabismus (i.e., esotropia), and loss of power to rotate the affected eye outward), and disruption of the facial nerves (CN VII) leads to symptoms including flaccid paralysis of the muscles of facial expression and loss of the corneal reflex. Disruption of the corticospinal tract leads to contralateral hemiplegia of the extremities (811).

 

Guillaume Benjamin Amand Duchenne (FR) developed a meticulous neurological examination to which he added electrical stimulation as a diagnostic test in localization. In 1855, he localized the lesion in polio to the anterior horn cells. His great book in 1867 was a kinesiology of the entire muscular system (563; 565; 566).

 

William Smellie (GB), Guillaume Benjamin Amand Duchenne (FR) and Wilhelm Heinrich Erb (DE) described Erb's or Erb–Duchenne palsy as a form of brachial plexus palsy most common in dystocia, an abnormal or difficult childbirth or labor (566; 628; 1764).

 

Christian Albert Theodor Billroth (DE-AT) wrote a monograph on colonic polyps recognizing the relationship between adenomatous polyps and colorectal cancers (160). He was the first surgeon to excise a rectal cancer and by 1876 he had performed 33 such operations.

 

Alexander Theodor von Middendorff (RU) wrote Die Isepiptesen Russlands, an account of bird migration in Russia (2045).

 

Matthew Fontaine Maury (US) wrote the first textbook on oceanography (1220).

 

1856-1870

A pandemic of diphtheria spreads into Europe, North America, and Australia. It coincides with a deficiency of rain in these regions (1338).

Victor John Fourgeaud (US), a San Francisco doctor and legislator, described a diphtheria epidemic in California (702).

 

1856

William Henry Perkin (GB) patented the aniline dye tyrian purple (mauve) (377).

Jakub Natanson (PL) heated aniline in the presence of chloride of ethylene resulting in a mixture that took on a rich blood-red color. This was most likely the dye rosaniline (basic fuchsine or magenta I) (1328).

Georg Christian August Wilhelm Hofmann (DE) obtained fuchsine by the reaction of carbon tetrachloride with aniline. Later he isolated rosaniline, and from it, produced a series of violet dyes including aniline blue (triphenyl rosaniline) (1993).

François-Emmanuel Verguin (FR), between 1858 and 1859, found that reaction of aniline with stannic chloride gave a fuchsia, or rose-colored dye, which he named fuchsin (magenta or roseine) (1529). Fuchsine is still in use as a biological stain, especially in the periodic acid-Schiff's reagent for detecting aldehydes.

Edward Chambers Nicholson (GB) discovered a better route to the red in which arsenic acid was employed in the oxidative condensation of commercial aniline. He called this dye roseine (905).

Henry Medlock (GB), in 1860, patented a process for making roseine almost identical to that of Nicholson.

Charles Girard (FR) and Georges de Laire (FR), in 1860, produced spirit-soluble blue (aniline blue or Lyon blue) by heating magenta with aniline (19).

 

Jean Baptiste Joseph Dieudonné Boussingault (FR) showed that nitrates are the most suitable form of nitrogen supply for plants (220). He was commemorated by the genus Boussingaultia.

 

Leon Semenowitj Cienkowski (PL-RU) convincingly demonstrated that morphological and physiological processes on the border of animals and plants coincide and no clear characters, delimitating the two kingdoms, can be found (376).

 

Arnold Cloetta (CH) discovered inosite, taurin, leucin, and uric acid in animal lung tissue (383; 384).

 

Francois Remy Lucien Corvisart (FR), in 1856, was the first to prove the digestion of proteins by pancreatic juice (trypsin) (439).

Alexander Jakovlevich Danilevsky; Alexander Jakovlevich Danielewski (RU) experimentally separated trypsin from pancreatic amylase by differential adsorption (475).

Wilhelm Friedrich Kühne (DE) coined the term enzyme (Greek énzymos = leavened) to describe the pancreatic protease (trypsin). He also coined the name trypsin (1044; 1045).

Wilhelm Friedrich Kühne (DE) found that an alkaline environment does, in fact, aid the proteolytic action of trypsin (1048).

John Howard Northrop (US) and Moses Kunitz (RU-US) were the first to crystallize the enzyme trypsin (1357-1361).

 

John William Draper (US) was one of the first to produce photomicrographs, taking pictures of what he saw under a microscope and reproducing them in a book on physiology (554).

 

Angelo Malestri (IT) and Emilio Cornalia (IT) were the first to describe inclusion viruses. They found the inclusion bodies in diseased silkworms (433; 1199).

 

Peter Ludwig Panum (DK) discovered bacterial endotoxin (1409; 1410).

Richard Friedrich Johannes Pfeiffer (DE) coined the term endotoxin to describe a substance produced by Gram-negative bacteria that could provoke fever and shock in experimental animals (1461).

Alexander Besredka (RU-FR) demonstrated that – provided appropriate immunization schedules were applied anti-endotoxin antibodies could be engendered which were capable of neutralizing the poisonous effects of endotoxin (144). Note: In the decades that followed, endotoxin was chemically characterized and identified as a lipopolysaccharide (LPS) produced by most Gram-negative bacteria.

 

Claude Bernard (FR) discovered the presence of a starch-like substance in the mammalian liver, which he called animal starch (glycogen). He identified animal starch as the source of endogenous glucose and noted that it is converted into maltose by the action of salivary enzyme, pancreatic juice, malt extract, and yeast cells (127; 133-135). Note: The liver's secretion of glucose into the blood stream in 1857d is the origin of the doctrine of internal secretion.

 

Rudolf Albert von Kölliker (CH) made the first detailed study of muscle cell granules or sarcosomes (later mitochondria) and recognized their wide distribution in the animal kingdom (2009-2012).

Carl Benda (DE) coined the name fädenkörner or mitochondrien (from the Greek meaning thread granule) to identify rod-like structures found in cytological preparations by Richard Altmann (DE). Altmann had called them bioblasts and regarded them as microorganisms; Benda recognized them as cellularß organs (27; 101). Today we call them mitochondria (sing. mitochondrium). Benda developed a staining technique using crystal violet and alizarin, which was especially good at demonstrating mitochondria (102).

Leonor Michaelis (DE-US) discovered that mitochondria can be stained selectively and supravitally with a dilute solution of Janus green, the method of choice until 1952 (1247).

Margaret Reed Lewis (US) and Warren Harmon Lewis (US) described and pondered the location of mitochondria within cells (1116).

 

William Starling Sullivant (US) and Leo Lesquereux (CH-US) wrote two important books on North American mosses (1818; 1819).

 

George Engelmann (DE-US) produced two outstanding monographs on the Cactaceae of the Southwestern United States and adjacent Mexico (626; 627).

 

Felix Joseph Henri de Lacaze-Duthiers (1856-57) reared scaphopod embryos (tusk shells) through 35 days of larval life and described the trochophore and veliger larvae (1058).

Alexander Onufrievich Kowalevsky (RU) later provided a more detailed description of the trochophore and veliger larvae (1038).

 

Maximillian Johann Sigismund Schultze (DE) localized mammalian olfactory receptors high in the nasal cavity in a small area in the superior turbinated bone where there resided special cells with hair-like processes. Because the area has a distinctive yellow color in humans it was given the name locus luteus (1706).

Bartolomeo Camillo Golgi (IT) identified the endings of the olfactory fibers, the granular cells, and the large mitral cells, along with the arborizations within each glomerulus (760-762).

David Ferrier (GB) concluded from experiments in monkeys that lesions in the temporal lobes often affected the sense of smell (649).

Santiago Ramón y Cajal (ES) found that the olfactory tract projects back without crossing to end primarily in the temporal prepyriform cortex and in the corticomedial nuclei of the amygdala (1544).

 

Charles-Édouard Brown-Séquard (FR) proved that removal of both suprarenal (adrenal) glands from dogs invariably led to an exaggerated Addison’s disease and inevitably death. This argued for ductless glands and the doctrine of internal secretion (308; 309).

 

Eduard Jäger von Jaxtthal (AT) was the first to report diabetic macular changes in the form of yellowish spots and extravasations that permeated part or the whole thickness of the retina (1997).

Edward Nettleship (GB) provided the first histopathological proof of "cystoid degeneration of the macula" in patients with diabetes (1334).

Wilhelm Manz (DE) described the proliferative changes occurring in diabetic retinopathy and the importance of tractional retinal detachments and vitreous hemorrhages (1204).

Arthur James Ballantyne (GB) and Arnold Lowenstein (IL) provided more evidence suggesting that diabetic retinopathy represents a unique vasculopathy (74).

 

F.M. von Heymann (DE) was the first to describe retinal cytoid bodies (in collaboration with Zenker in a case of Bright’s disease) thinking that they were degenerate ganglion cells (1991).

Heinrich Müller (DE) correctly claimed that retinal cytoid bodies were swollen varicose nerve fibers (1298).

 

Rudolf Albert von Kölliker (CH) and Heinrich Müller (DE) were the first to demonstrate that an electrical current accompanies each heartbeat. They measured the current by applying a galvanometer to the base and apex of an exposed ventricle of a frog’s heart. They also applied a nerve-muscle preparation to the ventricle and observed that a twitch of the muscle occurred just prior to ventricular systole and a much smaller twitch after systole (76; 2013). These twitches would later be recognized as being caused by the electrical currents of the QRS and T waves.

 

Edme Félix Alfred Vulpian (FR) applied a solution of ferric chloride to slices of the adrenal glands and noted that the medulla stained green while the cortex did not. He also noted that the same reaction was given by samples of venous blood leaving the adrenal, but not by arterial blood entering the gland. To account for these observations, he assumed that the medulla synthesized a substance that was liberated into the circulation (2065). That substance turned out to be epinephrine (adrenaline).

Berthold Werner (PL) reported a histochemical reaction associated with the adrenal medulla, the chromaffin reaction, named so for a relatively specific reaction to chromate salts, observed as a brownish deposit after fixation in chromic acid or dichromate salts (2117).

Alfred Kohn (CZ) coined the phrases chromaffin reaction and chromaffin cell (1029).

 

Heinrich Anton de Bary (DE), in 1856, while studying the fungus Sclerotina, demonstrated the action of exoenzyme on the host, and postulated the influence of the host substrate on pathogenicity (493).

 

Rudolph Ludwig Karl Virchow (DE) reported a case of pulmonary aspergillosis (1933).

 

Rudolph Ludwig Karl Virchow (DE) was the first to describe the atheromatous plaque. He viewed atherosclerosis as a vascular inflammatory process (1935).

Nikolaj Nikolajewitsch Anitschkow; Nikolay Nikolaevich Anichkov (RU) noted how high cholesterol levels promoted atherosclerosis (38).

Siegfried Josef Thannhauser (US) and Heinz Magendantz (US) were among the first to associate both atherosclerosis and xanthoma with high serum cholesterol levels (1828).

Robert E. Olson (US), Michael S. Brown (US) and Joseph S. Goldstein (US) reasoned that cholesterol per se is not the agent promoting athrosclerosis because low-density-lipoprotein (LDL), which contains cholesterol, is pro-atherogenic whereas high-density-lipoprotein (HDL), which also contains cholesterol, is anti-atherogenic (298; 1373).

 

Robert Remak (DE) discovered the motor points—the entry points of the nerves into the muscles – essential for stimulating the muscles by electricity (1600).

 

Jakob Ernst Arthur Böttcher (DE) described the nerve ganglion around the cochlear nerve within the internal auditory meatus (203).

 

Adolf Eugen Fick (DE) wrote Die Medizinische Physik, the first book in the world dedicated to medical physics (650).

 

Rudolph Ludwig Karl Virchow (DE) proposed the term erregbarkeit (excitability) for the cell’s capacity to respond to altered nutritive action and reizbarkeit (irritability) for the cells capacity to exhibit contraction, secretion, and conduction (1932).

 

William Budd (GB) published his first paper on typhoid fever. He concluded that the specific element was furnished in the excreta of affected individuals. "The first thing to attract attention after the disorder had become rife in North Tawton, was the strong tendency it showed, when once introduced into a family, to spread through the household." By his studies, Budd showed a high probability that the specific element was in the intestinal discharges from the fever patients and fresh cases occurred through contact with the feces.

In another series, "kept in strict separation from one another, as far as their persons were concerned, the common privy was almost the only connecting link left between them. Neither dirt nor rotting manure cause the fever but some specific element breeding and multiplying in the body and passing to well individuals by various routes." It was not the aesthetically objectionable and disagreeable rotting feces that were responsible for the spread of typhoid but some specific element in the stools from a previous case (323-325).

 

Austin Flint (US) was an exceptional teacher and medical diagnostician. Through his teaching and writing he had a profound positive influence on medicine in America. His books included: Physical Exploration and Diagnosis of Diseases Affecting the Respiratory Organs, Compendium of Percussion and Auscultation, A Manual of Percussion and Auscultation, A Practical Treatise on the Diagnosis, Pathology, and Treatment of Diseases of the Heart, and A Treatise on the Principles and Practice of Medicine (690-694).

 

William Withey Gull (GB) was one of the first to describe the pathological lesions in tabes dorsalis (failure of muscular coordination) and intermittent hemoglobinuria. He and Henry Gawen Sutton (GB) described arterio-capillary fibrosis in chronic nephritis, called Gull-Sutton disease (815). See, Romberg, 1840.

 

In 1856, bones were discovered in a cave in the Neander River Valley near Düsseldorf, Germany by quarrymen who gave them to a local schoolteacher and amateur naturalist, Johan Karl Fuhlrott. Fuhlrott identified them as human and thought them to be very old. He recognized them to be different from the usual bones of humans and showed them to the Professor of Anatomy at the University of Bonn, Hermann Schaaffhausen. Fuhlrott and Schaaffhausen presented papers on the fossils and the geology of the Feldhofer Cave at a meeting of the Niederrheinische Gesellschaft für Natur- und Heilkunde (Lower Rhine Medical and Natural History Society) in Bonn in 1857. They published independently at a later date (718; 1674).

William King, professor of geology at Queens College in Galway, Ireland, presented a paper in 1864 where he argued the Neanderthal fossils of Fuhlrott and Schaaffhausen belonged to an extinct species of early human that he named Homo neanderthalensis (Homo sapiens neanderthalensis). He named them for the Neander River Valley (tal = river in German) (1007; 1008). Note: This specimen probably lived around 80K B.C.E.

 

1857

"Disease is from of old and nothing about it has changed. It is we who change, as we learn to recognize what was formerly imperceptible." Jean-Martin Charcot (FR) (360)

 

Claude-Félix-Abel Niepce de Saint-Victor (FR) noticed a fogging of silver chloride emulsions by uranium salts. He reported that the blackening of the emulsions occurred even when they were separated from the uranium salts by thin sheets of paper. He did not appreciate the true nature of the phenomenon that probably represents the first observation of radioactivity. Even in that early period, Niepce de Saint-Victor already knew that the radioactive emission was spontaneous, and not due to phosphorescence because it was detectable even months after exposure to sunlight. Note: A case can be made that Niepce de Saint-Victor discovered radioactivity 40 years before Becquerel(1346-1349).

 

Eduard Schweizer (CH) developed a solution of cupric oxide in ammonia, which would dissolve cellulose without decomposing it (1726).

 

Franz von Leydig (DE) was the first person to state in unequivocal terms that the cell wall is not a necessary constituent of the cell. “. . . not all cells are of bladder-like nature; a membrane separable from the contents is not always distinguishable. For the morphological idea of a cell one requires a more or less soft substance, primitively approaching a sphere in shape, and containing a central body called a kernel (nucleus). The cell-substance often hardens to a more or less independent boundary-layer or membrane, and the cell then resolves itself, according to the terminology of scholars, into membrane, cell contents, and kernel (2019). Note: This book gives the best account of the rapid growth of comparative microscopical anatomy in the two decades following Schwann’s discoveries.

 

Charles Edward Isaacs (US) discovered that, “… the Malpighian tuft or coil is covered by oval, nucleated cells, which are differently affected by chemical reagents from those which line the capsule, and consequently have a different organization. The Malpighian tuft is evidently, then, a glandular structure, every way adapted for the separation of the proximate elements of the urine…” (964). Note: Malpighian tuft or coil = glomerulus

 

Georg Meissner (DE) discovered the submucosal nervous plexus consisting of small aggregations of ganglion cells, innervating the submucosa of the alimentary tract (1237). These became known as Meissner’s plexus.

 

Karl Wilhelm Nägeli (CH) introduced the term Schizomycetes (fission fungi) as a collective designation for the bacteria (1323).

 

Louis Pasteur (FR) produced fermentation in a solution, which did not contain gluten (albumin). He showed that in a solution of pure sugar with a small quantity of ammonium phosphate and chalk, cloudiness appears, and gas was evolved. As the fermentation proceeded the ammonia disappeared, phosphates and calcium salts were dissolved, lactate of calcium was formed, and lactic yeast settled to the bottom. He showed that in all probability the lactic ferment came from the air, for when he used sterile solutions of the various ingredients and allowed only heated air to enter, neither lactic fermentation nor lactic acid nor infusoria appeared; the fluid remained barren. He also showed that some of the sugar was incorporated into cellular material (1426; 1427).

 

Jean Baptiste Payer (FR) wrote an outstanding treatise on floral anatomy (1435). His work along with that of van Tieghem below represents the origin of the scholarly treatment of floral anatomy.

Philippe Edouard Léon van Tieghem (FR) defined the plant as having three distinct parts, the stem, the root, and the leaf. He studied the origin and differentiation of each type of plant tissue. He studied the gross anatomy of the phanerogams (plants with reproductive organs) and the cryptogams (plants without reproductive organs), such as mosses and ferns. Van Tieghem created a plant anatomy founded on the homologies of tissues and on their origin from the initial cells (1643; 1904-1908).

 

Franz von Leydig (DE) described a large secretory cell, found in the epidermis of fishes and larval amphibians. This mucous cell is peculiar in that it does not pour its secretion over the surface of the epithelium. Von Leydig believed that its function was to lubricate the skin; the cell now bears his name (2019).

 

Karl Wilhelm Nägeli (CH) described spores that were probably Nosma bombycis, a microsporidian, when he investigated an outbreak of a disease called pébrine in the silkworm, Bombyx mori (1322).

Édouard-Gérard Balbiani (FR) placed Nosema bombysis, the causative agent of pébrine a disease of silkworms, in the class Sporozoa (73).

 

Friedrich August Brauell (DE), a professor of veterinary medicine, carried out several inoculations to demonstrate the transmissibility of anthrax to sheep by means of human or horse anthrax blood. Dogs and fowl were found to resist infection. He found rod shaped objects in the anthrax bloods but did not regard them as unique to anthrax (238; 239).

 

David Livingstone (GB) is credited with the first account in 1857 of a malady associated with the bite of soft ticks in Angola and Mozambique (1129).

Otto Obermeier (DE), in 1873, first described the disease-causing ability and mechanisms of spirochetes, but was unable to reproduce relapsing fever in inoculated test subjects and thereby unable to fulfill Koch's postulates (456).

Relapsing fever was not successfully produced in an inoculated subject until 1874 (456).

Albert R. Cook ( GB), Joseph Everett Dutton (GB), John Lancelot Todd (GB), Philip H. Ross (GB), A.D. Milne (GB), Joseph Everett Dutton (GB), John Lancelot Todd (GB), and F.C. Wellman (GB) outlined the cause of relapsing fever and its relationship with ticks (420; 602; 1627; 2113).

Frederick George Novy (US) and Rudolph E. Knapp (US) reported the cause of tick-borne relapsing fever across central Africa was Spirillum duttoni (1364). Note: This microorganism was subsequently named Spirillum duttoni, then Borrelia duttoni .

 

Willoughby F. Wade (GB) and Julius Jacobson (DE) characterized cases of syphilitic retinitis (973; 2067).

 

Charles Locock (GB), obstetrician to Queen Victoria, was reported to have commented that during the past 14 months he had used potassium bromide to successfully stop epileptic seizures in all but one of 14 or 15 women with hysterical or catamenial epilepsy at a meeting of the Royal Medical and Chirurgical Society in London on Tuesday 11 May 1857. Edward H. Sieveking (GB) had just previously presented 52 cases of epilepsy (1743).

 

Claude Bernard (FR) found that glucose production continued in diabetic cases where glycogen stores had been depleted. This was the first recognition of the process of gluconeogenesis (133).

 

Friedrich Wilhelm Ernst Albrecht von Graefe (DE) justified iridectomy for glaucoma as follows: “It seemed to me that all the characteristic symptoms [of glaucoma] tended to one point—increase of the intra-ocular pressure…. Supported by these facts and considerations, I considered myself perfectly justified in performing iridectomy in glaucoma; for I knew the favorable action of the operation on the condition of the choroid in regard to its circulation” (1979). In his short career von Graefe performed more than 10,000 eye operations. He died at 42 as undoubtedly the most important ophthalmologist of the 19th century.

 

Nikolaus Friedreich (DE) gave the first description of acute leukemia (713).

 

Thomas Henry Huxley (GB) insisted that as suggestive as they are, comparisons of adult structures are insufficient for the demonstration of homology in animals. Only by studying the embryonic development of the various structures from their earliest stages and determining that they follow the same path of development can we say with certainty that they are homologous (949).

 

Per Henrik Malmsten (SE) described the parasitic ciliated protozoan Balantidium coli, which was later named by Friederich von Stein (DE) (1201; 2063). Balantidium is the only ciliated protozoan known to infect humans. Balantidiasis is a zoonotic disease and is acquired by humans via the fecal oral route from the normal host, the pig, where it is asymptomatic.

 

Marshall Hall (GB) devised a method of artificial respiration. The subject was first placed in the prone position and pressed upon the back, causing an active expiration. He was then turned over on his side, with the shoulder raised, to bring about an active inspiration (826; 1773).

Henry Robert Silvester (GB) developed a new method of resuscitating stillborn children, and for restoring persons apparently drowned or dead. The patient lies on his or her back, with arms raised to the sides of the head, held there temporarily, then brought down and pressed against the chest. Movement repeated 16 times per minute (1744).

Edward Albert Sharpey-Schafer (GB) developed a method for artificial respiration of those who were apparently drowned. Place patient prone and face down turning their head to the side. Kneel across the patient’s waist placing both hands flat on the patient’s lower back. Gradually throw your weight forward onto the patient. Immediately thereafter the operator raises his body slowly to remove the pressure leaving his hands in place. This forward and backward motion is repeated every four to five seconds (1736).

 

Robert Bentley Todd (IE-GB) was the first to describe hypertrophic cirrhosis of the liver accurately, sometimes called Todd’s Disease (1863).

 

Rocco Gritti (IT) described knee disarticulation using the patella as a protective flap (1659).

 

The journal Jahrbücher für Wissenschaftliche Botanik was founded.

 

The city of Uruk, also known as Warka or Warkah, in present day Iraq, is given credit for being the first true city of human civilization. Uruk was the main force of urbanization and state formation during the Uruk period, or 'Uruk expansion' (4000–3200 B.C.E.) in the country of Sumer in the mid-4th millennium B.C.E. William Kennett Loftus (GB) visited the site of Uruk in 1849, identifying it as "Erech", known as "the second city of Nimrod", and led the first excavations from 1850 to 1854 (51; 1132). Note: The legendary king Gilgamesh, according to the chronology presented in the Sumerian King List, ruled Uruk in the 27th century B.C.E.; writing may have originated in Uruk around 3300 BC.

 

English country names and code elements taken from the International Organization for Standardization:

DZ = Algerian; US = American; AR = Argentinian; AU = Australian; AT = Austrian; AT/HU = Austro/Hungarian; BA = Bosnian-Herzegovinian; BE = Belgian; BR = Brazilian; GB = British; BG = Bulgarian; CM = Cameroonian; CA = Canadian; TD = Chadian; CL = Chilean; CN = Chinese; CO = Colombian; CR = Costa Rican; HR = Croatian; CU = Cuban; CY = Cypriot; CZ = Czechoslovakian; DK = Danish; NL = Dutch; EC = Ecuadorian; EG = Egyptian; EE = Estonian; ET = Ethiopian; FI = Finnish; FR = French; DE = German; GR = Greek; GT = Guatemalan; GU = Guamanian; HU = Hungarian; IS = Icelander; IN = Indian; ID = Indonesian; IR = Iranian; IQ = Iraqi; IL = Israeli; IE = Irish; IT = Italian; JP = Japanese; KE = Kenyan; KR = South Korean; KW = Kuwaiti ; LV = Latvian; LB = Lebanese; LT = Lithuanian; LU = Luxembourgian; MK= Macedonian; MG = Malagasy; MT = Maltese; MY = Malaysian; MX = Mexican; NA = Namibian; NZ = New Zealander; NG = Nigerian; NO = Norwegian; PK = Pakistani; PA = Panamanian; PE = Peruvian; PH = Filipino; PL = Polish; PT = Portuguese; PR = Puerto Rican; RO = Romanian; RU = Russian; SA = Saudi Arabian; SN = Senegalese; CS = Serbian-Montenegrin; SK = Slovakian; ZA = South African; ES = Spanish; LK = Sri Lankan; SE = Swedish; CH = Swiss; SY = Syrian; TW = Taiwanese; TH = Thai; TN = Tunisian; TR = Turkish; UG = Ugandan; UA = Ukrainian; UY = Uruguayan; VE = Venezuelan; ZW = Zimbabwean

 

 

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122.     Bernard C. 1849c. Influence du système nerveux sur la production du sucre dans l'économie animale [Influence of the nervous system in the production of sugar in the animal economy]. Ext. Proces-Verbaux Seanes Soc. Philomat. Paris:49-51

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131.     Bernard C. 1856d. Lecons de Physiologie Expérimentale Appliquées à la Médecine [Lessons of Experimental Physiology Applied to Medicine]. Paris: J.B. Ballière et Fils. 510 pp.

132.     Bernard C. 1857a. Lecons sur les Effets des Substances Toxiques et Médicamenteuses [Lessons on the Effects of Toxic Substances and Drugs]. Paris: J.-B. Baillière et fils. 488 pp.

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1933.   Virchow RLK. 1856b. Beiträge zur lehre von dem beim menschen vorkommenden pflanzlichen parasiten [Contributions to the teaching of the occurring when people plant parasites]. Arch. Path. Anat. Physiol. Klin. Med. 9:557-93

1934.   Virchow RLK. 1856c. Die Leukemia [The Leukemia]. In Gesammelte Abhandlungen zur Wissenschaftlichen Medizin [Collected Essays on Scientific Medicine], ed. RLK Virchow. Frankfurt: Meidinger. Number of.

1935.   Virchow RLK. 1856e. Der atheromatös prozess der arterien [The atheromatous process of the arteries]. Wien. Med. Wochenschr. 6:809-12, 25-27

1936.   Virchow RLK. 1858a. Die Cellularpathologie in Ihrer Begründung auf Physiologische und Pathologische Gewebelehre [Cellular Pathology as Based Upon Physiological and Pathological Histology]. Berlin: Verlag von August Hirschwald. 440 pp.

1937.   Virchow RLK. 1859. Recherches sur le développement du Trichina spiralis [Research on the development of Trichina spiralis]. C.R. Acad. Sci., Paris 49:660-2

1938.   Virchow RLK. 1860a. Cellular Pathology as Based Upon Physiological and Pathological Histology. London: Churchill. 511 pp.

1939.   Virchow RLK. 1910. Thrombose und Embolie (1846-1856) [Thrombosis and Embolism (1846-1856)]. Leipzig: J.A. Barth. 237 pp.

1940.   Virey J-J. 1814. Éphémérides de la Vie Humaine. Medical Faculty of Paris, Paris

1941.   Vogel HA. 1815. Versuche über die zersetzung der salze und der metalloxyde durch den zuckerstoff [Experiments on the decomposition of the salts and metallic oxides by the sugar substance]. J. Chem. Phys. 13:162-

1942.   Vogel J. 1838. Physiologisch-Pathologische Untersuchungen über Eiter, Eiterung und die Damit Verwandten Vorgänge [Physiological - Pathological Studies of Pus ,Suppuration and its Related Operations]. Erlangen: Palm & Enke. 238 pp.

1943.   Vogel J. 1843. Icones Histologiae Pathologicae. Tabulae Histologiam Pathologicam Illustrantes [Images of Histology and Pathology. Tables Histologie Pathologic Illustranted]. Lipsiae: Voss. 26 pp.

1944.   Vogt C. 1851. Zoologische Briefe, Naturgeschichte der Lebenden und Untergegangen Thiere [Zoological Letters: Natural History of the Extant and Extinct Animals]. Frankfurt

1945.   Volterra V. 1926. Fluctuations in the abundance of a species considered mathematically. Nature 118:558-60

1946.   von Alberti FA. 1834. Beitrag zu einer Monographie des Bunten  Sandsteins, Muschelkalks und Keupers, und die Verbindung dieser Gebilde  zu einer Formation [Contributions to a Monograph of Colorful Sandstone, Mussel Chalk and Keuper, and Connecting These Structures to a Formation]. Stuttgart und Tübingen: J.G. Cotta. 366 pp.

1947.   von Babo CHL. 1852. Mittheilungen aus dem chemischen laboratorium zu Freiburg. 1. Über die anwendung der centrifugalkraft im chemischen laboratorium [Mittheilungen from the chemical laboratory to Freiburg. 1. From the application of centrifugal force in the chemical laboratory]. Ann. Chem. Pharm. 82:301-11

1948.   von Babo LHJ. 1847. Ueber die Spannkraft des Wasserdampfes in Salzlösungen. Ein Beitrag zur Statik der Atome [About the Clamping Force of the Water Vapor in Salt Solutions. A Contribution to the Statics of the Atoms]. Freiburg. 32 pp.

1949.   von Baer KE. 1827. De Ovi Mammalium et Hominis Genesi [On the Genesis of the Ovum of Mammals and of Men]. Epistola ad Academiam Imperialem Scientiarum Petropolitanum. Lipsiae: Leopold Vossi. 40 pp.

1950.   von Baer KE. 1828. Commentar zu der Schrift: De ovi mammalium et hominis genesi. Epistola ad academiam scientiarum Petropolitanam [Commentary to the scriptures: The mammalium egg and a man's birth chart. Letter to the Academiam Imperialem Scientiarum Petropolitanam]. Z. Organ. Phys. 2:125-93

1951.   von Baer KE. 1828-1837. Uber Entwickelungsgeschichte der Thiere, Beobachtungen und Reflexionen  [On the Developmental History of Animals, Observation and Reflection ]. Konigsberg: Gebrüder Bornträger

1952.   von Baer KE. 1931. The Discovery of the Mammalian Egg and the Foundation of Modern Embryology. Bruges: St. Catherine Press. 330 pp.

1953.   von Baer KE, O'Malley CD. 1956. On the genesis of the ovum of mammals and of man. Isis 47:117-53

1954.   von Basch SSK. 1893. Ueber Latente Arteriosclerose und Deren Beziehung zu Fettleibigkeit, Herzerkrankungen und Anderen Begleiterscheinungen [About Latent Arteriosclerosis and its Relation to Obesity, Heart Disease, and Other Side Effects]. Viena: Urban & Schwartzenberg. 43 pp.

1955.   von Bergmann EGB. 1882a. Die gruppierung der wundkrankheiten [The grouping of the miracle diseases]. Berlin. Klin. Wchnschr. 19:677-9, 701-3

1956.   von Bergmann EGB. 1882b. Ueber antiseptische wundbehandlung [About antiseptic wound treatment]. Dtsch. Med. Wochenschr. 8:559-61, 71-72

1957.   von Bergmann EGB. 1889. Die Chirurgische Behandlung von Hirnkrankheiten [The Surgical Treatment of Brain Diseases]. Berlin: August Hirshwald. 114 pp.

1958.   von Berzelius JJ. 1830. On the composition of tartaric acid and racemic acid (John's Acid from the Vosges Mountains), on the atomic weight of lead oxide, together with general remarks on those substances with have the same composition but different properties. Pogg. Ann. 19, series 1:326

1959.   von Berzelius JJ. 1833. Calculus Urinates [Urinary Stones]. In Traité de Chimie [Treatise of Chemistry], 7:424-. Paris: F. Didot Number of 424- pp.

1960.   von Berzelius JJ. 1833. Traité de Chimie [Treatise on Chemistry]. pp 156. Paris: Didot Ferès

1961.   von Berzelius JJ. 1836a. Considerations respecting a new power which acts in the formation of organic bodies. New Edinb. Philosoph. Journal 21:223-8

1962.   von Berzelius JJ. 1836b. Einige ideen über eine bei der bildung organischer verbindungen in der lebenden natur wirksame, aber bisher nicht bemerkte kraft [Some ideas about organic compounds effective in the formation of living nature, but not yet observed as such]. Jahresber. Fortschr. Physisc. Wiss. 15:237-45

1963.   von Berzelius JJ. 1836c. Quelques idées sur une nouvelle force agissant dans les combinaisons des corps organiques [Some ideas on a new force acting in combinations of organic bodies]. Ann. de Chim. et de Phys. 61:146-51

1964.   von Berzelius JJ. 1836d. Einige ideen über bei der bildung organischer verbindungen in der lebenden natur wirksame, aber bisher nicht bemerkte kraft [Some ideas about education in the organic compounds effective in life forms, but so far not commented upon]. Jahresber. Fortschr. Physisc. Wiss. 15:237-45

1965.   von Berzelius JJ. 1838. Undersokning af Nagra Syror, som Bildas af Organiska Amnen med Svafvelsyra [Investigation of Some Acids, Formed by Organic Substances with Sulfuric Acid]. Stockholm. 70 pp.

1966.   von Berzelius JJ, Wöhler F, Braun J, Wallach O. 1901. Briefwechsel Zwischen J. Berzelius und F. Wöhler [Correspondence Between J. Berzelius and F. Wöhler]. Leipzig: W. Engelmann. 717 pp.

1967.   von Chamisso LA. 1819. De Animalibus Quibusdam e Classe Vermium Linnaeana in Circumnavigatione Terrae : Auspicante Com [Some of the Animals From the Class Vermium Linnaeana in Circumnavigation of the Earth: With Sponsor]. Berolini: Apud Ferd. Dümmlerum

1968.   von Eschscholtz JFG. 1829. System der Acalephen: Eine aus Führliche Beschreibung aller Medusenartigen Strahlthiere [System of Acalephen: A Detailed Description of all Medusa-like Animals of Prey]. Berlin: F. Dummlert. 190 pp.

1969.   von Eschscholtz JFG, Rathke MH. 1829-1833. Zoologischer Atlas [Zoology Atlas] Berlin

1970.   von Euler-Chelpin E, von Euler-Chelpin HKAS, Hellström H. 1928. A-vitaminwirkungen der lipochrome [A vitamin-effects of lipochrome]. Biochem. Z. 203:370-84

1971.   von Feuchtersleben E. 1845. Lehrbuch der Ärztlichen Seelekunde. Als Skizze zu Vortragen Bearbeitet von Dr. Ernst Freiherrn von Feuchtersleben [The Principles of Medical Psychology]. pp 429. Wien: Druck und Verlag von Carl Gerold

1972.   von Frankl-Hochwart L. 1891. Die Tetanie [The Tetany]. Berlin: Hirschwald. 134 pp.

1973.   von Fraunhofer J. 1898. Prismatic and Diffraction Spectra; Memoirs by Joseph von Fraunhofer. New York, London: Harper & brothers. 67 pp.

1974.   von Frerichs FT. 1849. Über hirnsklerose [About cerebral sclerosis]. Arch. Ges. Med. 10:334-50

1975.   von Frerichs FT. 1851. Die Bright’sche Nierenkrankheit und deren Behandlung [The Bright's Kidney Disease and its Treatment]. Braunschweig: FriedrichVieweg und Sohn. 286 pp.

1976.   von Gerlach J. 1851. Ueber das hautathmen [About the hautathmen]. Arch. Anat. Physiol. Wiss. Med.:431-55

1977.   von Goethe JW. 1817. Zur Morphologie [On Morphology]. Stüttgart: J.G. Cotta. 96 pp.

1978.   von Gorup-Besánez EF, Will H. 1876. Fortgesetzte beobachtungen über peptonbildende fermente im pflanzenreiche [Continued observations on the peptone-forming ferments in the plant kingdom]. Ber. Dtsch. Chem. Ges. 9:673-8

1979.   von Graefe FWEA. 1857. Die iridectomie bei glaucom und über den glaucomatösen process [Iridectomy for glaucoma and the glaucomatous process]. Arch. Ophthalmol. 3:456-560

1980.   von Graefe KF. 1970. Carl Ferdinand von Graefe (1787-1840). Plast. Reconstr. Surg. 46:554-63

1981.   von Grotthuss TCJD. 1819. Über die chemische wirksamheit des lichts und der elektricität…[On the chemical action of light and of electricity]. Ann. Phys. und Physik. Chem. 61:50-74

1982.   von Hansemann DP. 1890. Über asymmetrische zelltheilung in epithel krebsen und deren biologische bedeutung [About asymmetric cell division in epithelial cancers and its biological significance]. Arch. Path. Anat. Physiol. Klin. Med. 119:299

1983.   von Helmholtz HLF. 1842. De Fabrica Systematis Nervosi Evertebratorum [The Structure of the Nervous System in Invertebrates]. dissertation. The Royal Friedrich-Wilhelm Institute for Medicine and Surgery, Berlin. 29 pp.

1984.   von Helmholtz HLF. 1847. Über die Erhaltung der Kraft, eine Physikalische Abhandlung : Vorgetragen in der Sitzung der Physikalischen Gesellschaft zu Berlin am 23sten Juli 1847  [On the Conservation of Energy]. Berlin: G.A. Reimer. 72 pp.

1985.   von Helmholtz HLF. 1848. Report on work done on the theory of animal heat in 1846. Fortschr. Physik

1986.   von Helmholtz HLF. 1850b. Über die fortpflanzungsgeschwindigkeit der nervenreizung [About the reproduction speed of nerve irritation]. Arch. Anat. Physiol. Wiss. Med.:71-3

1987.   von Helmholtz HLF. 1851. Beschreibung Eines Augen-Spiegels zur Untersuchung der Netzhaut im Lebenden Auge [Description of an Ophthalmoscope for the Investigation of the Retina in the Living Eye]. Berlin: Förstner. 43 pp.

1988.   von Helmholtz HLF. 1852. Measurement of the rate of transmission of the excitatory process in nerve. Arch. Anat. Physiol. Wiss. Med.:88

1989.   von Helmholtz HLF. 1954. On the Sensations of Tone as a Physiological Basis for the Theory of Music [Die Lehre von den Tonempfindungen als Grundlage für die Theorie der Musik] New York: Dover Publications. 576 pp.

1990.   von Helmholtz HLF. 1962. Helmholtz's Treatise on Physiological Optics [Handbuch der Physiologischen Optik. English] New York: Dover Publications

1991.   von Heymann FM. 1856a. Posterior sclerotico-choroiditis. Archiv. Ophthalm. 2:131-6

1992.   von Heymann FM. 1856b. Ueber amaurose bei brightischer krankheit, und fettdegeneration der netzhaut [About amaurosis in bright's disease, and fat degeneration of the retina]. Arch. Ophthalmol. 2:137-50

1993.   von Hofmann AW. 1858. Recherches pour servir á l'histoire des bases organiques [Contributins to the history of the organic bases]. C.R. Acad. Sci., Paris 47:492-8

1994.   von Humboldt FWHA. 1848. Cosmos: Sketch of a Physical Description of the Universe. pp 76. London: Henry G. Bohn

1995.   von Humboldt FWHA. 1849. Aspects of Nature in Different Lands and Different Climets with Scientific Elucidations. Philadelphia, PA: Lea and Blanchard

1996.   von Humboldt FWHA, Bonpland A. 1852. Personal Narrative of Travels to the Equinoctial Regions of America, During the Years 1799-1804. London: Henry G. Bohn

1997.   von Jaxtthal EJ. 1855-1856. Beitrage zur Pathologie des Auges [Contributions to the Pathology of the Eye]. Wien: Seidel. 33 pp.

1998.   von Kölliker RA. 1841. Beiträge zur Kenntniss der Geschlechtsverhältnisse und der Samenflüssigkeit wirbelloser Thiere, nebst einem Versuch über das Wesen und die Bedeutung der sogenannten Samenthiere [Contributions to the Knowledge of Gender Relations and the Semen of Invertebrate Animals , Together with an Essay on the Nature and Importance of the So-called Seed Animals]. Berlin: Logier. 88 pp.

1999.   von Kölliker RA. 1844. Die Selbständigkeit und Abhängigkeit des Sympathischen Nervensystems, durch Anatomische Beobachtungen Bewiesen von A. Kölliker. Ein Academisches Programm [The Independence and Dependence of the Sympathetic Nervous system, by Anatomical Observations Proved by A. Kölliker . An Academic Program]. Zurich: Im Verlage von Meyer und Zeller. 40 pp.

2000.   von Kölliker RA. 1845. Die lehre von der tierischen zelle [The teaching of the animal cell]. Z. Wiss. Bot.:46-102

2001.   von Kölliker RA. 1847a. Über die struktur un die verbreitung der glatten oder unwillkürlichen muskeln [About the structure and proliferation of smooth or involuntary muscles]. Mitt. Naturf. Ges. Zurich 1:18-28

2002.   von Kölliker RA. 1847b. Die bildung der samenfäden in bläschen als allgemeines entwicklungsgesetz [Creation of common threads in bubble as a act of general development]. Neue Denkschriften der Allgemeinen Schweizerischen Gesellschaft für die Gesammten Naturwissenschaften 8:1

2003.   von Kölliker RA. 1848a. Beiträge zur kenntniss niederer thiere [Contributions to the knowledge of the lower animals]. Z. Wiss. Zool. 1:1-37

2004.   von Kölliker RA. 1848b. Das sonnentierchen, Actinophrys sol. [The little animals of the sun , Actinophrys sol .]. Z. Wiss. Zool. 1:198-217

2005.   von Kölliker RA. 1849. Neurologische Bermerkungen [Neurological Comments]. Z. Wiss. Zool. 1:135-63

2006.   von Kölliker RA. 1852a. Handbuch der Gewebelehre des Menschen. Für Aerzte und Studirende [Handbook of Histology of Man. Made For Physicians to Study]. Leipzig: W. Engelmann. 637 pp.

2007.   von Kölliker RA. 1852b. Zur anatomie und physiologie der retina [At the anatomy and physiology of the retina]. Verh. Phys.-Med. Ges. Würzburg 3:316-36

2008.   von Kölliker RA. 1852c. Mikroskopische Anatomie oder Gewebelehre des Menschen [Microscopic Anatomy and Histology of the Human]. Leipzig: Engelmann. 346 pp.

2009.   von Kölliker RA. 1857. Einige bemerkungen über die endigungen der hautnerven und den bau der muskeln [Some remarks about the terminations of the skin nerves and the construction of the muscles]. Z. Wiss. Zool. 8:311-25

2010.   von Kölliker RA. 1888a. Ueber den bau der quergestreiften muskelfasern [About the construction of striated muscle fibers]. Sitzber. Phys.-Med. Ges. Wurzburg 6:132-8

2011.   von Kölliker RA. 1888b. Zur kenntnis der quergestreiften muskelfasern [To know the striated muscle fibers]. Z. Wiss. Zool. 47:689-710

2012.   von Kölliker RA. 1896. Handbuch der Gewebelehre des Menschen [Manual of Human Histology]. Leipzig: W. Engelmann. 874 pp.

2013.   von Kölliker RA, Müller H. 1856. Zweiter bericht über die im jahr 1854/55 in der physiologischen anstalt der Universität Wurzburg angestellten versuche. VII. Nachweis der negativen schwankung des muskelstroms am naturlich sich kontrahierenden herzen [Second report on the employed in the physiological institution of the University of Wurzburg in the 1854/55 year trying . The course is the heart twitch VII . Evidence of negative fluctuation of the muscle-stream]. Verh. Phys.-Med. Ges. Würzburg 6:528-33

2014.   von Kries JA. 1895. Über die natur gewisser mit den psychischen vorgangen verknupfter gehirnzustande [About the nature of certain of the altered mental processes and the associated brain state]. Z. Psychol. 8:1-33

2015.   von Langenbeck BRK. 1839. Auffindung von pilzen auf der schleimhaut der speiseröhre einer typhus leiche [Finding of fungi on the mucous membrane of the gullet of a typhoid fever corpse]. Froriep's Notizen 12:145-7

2016.   von Leydig F. 1849-1850. Einige bemerkungen über die entwicklung der blattläuse[Some remarks on the development of aphids]. Z. Wiss. Zool. 2:62-6

2017.   von Leydig F. 1850. Zur anatomie der männlichen geschlechtsorgane und analdrüsen der säugetiere [At the anatomy of the male reproductive and anal glands of mammals]. Z. Wiss. Zool. 2:1-57

2018.   von Leydig F. 1854. Zur anatomie von Coccus hesperidum [The anatomy of Coccus hesperidum]. Z. Wiss. Zool. 5:1-12

2019.   von Leydig F. 1857. Lehrbuch der Histologie des Menschen und der Thiere [Textbook of Histology of Man and Animals]. Frankfurt-am-Main: Meidinger. 551 pp.

2020.   von Liebig JJ. 1830. Sur'acide contenu dans l'urine des quadrupèdes herbivores [The acid content in the urine of herbivorous quadrupeds]. Ann. de Chim. et de Phys. 43:188-98

2021.   von Liebig JJ. 1831. Sur un nouvel appareil pour l'analyse des substances organiques; et sur la composition de quelques-unes de ces substances [A new device for the analysis of organic substances; and the composition of some of these substances]. Ann. de Chim. et de Phys. 47:147-97

2022.   von Liebig JJ. 1832a. Sur les combinaisons produites par l'action du chlore sur l'alcool, l'ether, le gaz oléfiant et l'esprit acétique [The combinations produced by the action of chlorine on alcohol ether, olefiant gas and acetic spirit]. Ann. de Chim. et de Phys. 49:146-204

2023.   von Liebig JJ. 1832b. Über die verbindungen welche durch die einwirkung des chlors auf alcohol, aether, ölbildendes gas und essiggeist entstehen [Arise via the connections represented by the action of chlorine on alcohol, ether, oil forming gas, and vinegar spirit]. Ann. Pharm. 1:182-230

2024.   von Liebig JJ. 1834. Analyse der Harnsäure [Analysis of uric acid]. Ann. Pharm. 10:47-8

2025.   von Liebig JJ. 1835a. Sur les produits de l'oxidation de l'alcool [Products of the oxidation of the alcohol]. Ann. de Chim. et de Phys. 59:289-327

2026.   von Liebig JJ. 1835b. Über die produkte der oxydation des alcohols [About the products of oxidation of alcohols]. Ann. Pharm. 14:133-7

2027.   von Liebig JJ. 1837a. Anleitung zur Analyse Organischer Körper [Introduction to the Analysis of Organic Substances]. Braunschweig: F. Vieweg und Sohn. 72 pp.

2028.   von Liebig JJ. 1837b. Über die theorie des essigbildungsprocesses [About the theory of the process of vinegar formation]. J. Prakt. Chem. 12:22

2029.   von Liebig JJ. 1837c. Milchsäure, die säure des sauerkrauts [Lactic acid, the acid of sauerkrauts]. Ann. Pharm. 23:113-5

2030.   von Liebig JJ. 1839. Über die erscheinungen der gährung, fäulniss und verwesung, und ihre ursachen [Concerning the phenomena of fermentation, putrefaction, and decay, and their causes]. Pogg. Ann. 48, series 2:106-50

2031.   von Liebig JJ. 1840. Die Organische Chemie in Ihrer Anwendung auf Agricultur und Physiologie [Organic Chemistry in its Applications to Agriculture and Physiology] Braunschweig: F. Vieweg und Sohn. 352 pp.

2032.   von Liebig JJ. 1842a. Die Organische Chemie in Ihrer Anwendung auf Physiologie und Pathologie [The Animal Chemistry or Organic Chemistry in Application to Physiology and Pathology]. Braunschweig: F. Vieweg und Sohn. 342 pp.

2033.   von Liebig JJ. 1842b. Animal Chemistry: or Chemistry in its Applications to Physiology and Pathology. London: Taylor and Walton. 354 pp.

2034.   von Liebig JJ. 1843a. Die Thier-Chemie Oder die Organische Chemie in Ihrer Anwendung auf Physiologie und Pathologie [The Animal Chemistry or Organic Chemistry in Their Application to Physiology and Pathology]. Braunschweig: F. Vieweg und Sohn. 344 pp.

2035.   von Liebig JJ. 1843b. Animal Chemistry, or Organic Chemistry in its Application to Physiology and Pathology. 111 pp.

2036.   von Liebig JJ. 1844. Bemerkungen uber das Verhältniss der Thierchemie zur Thier -Physiologie [Remarks About the Ratio of Animal Chemistry to Animal Physiology]. In Reden und Abhandlungen [Speeches and Treatises], ed. Jv Liebig:48-82. Leipzig and Heidelerg: C.F. Winter’sche Verlagshandlung. Number of 48-82 pp.

2037.   von Liebig JJ. 1846. Baldriansäure und ein neuer körper aus käsestoff [Valeric acid and a new body made of cheese cloth]. Ann. Chem. Pharm. 57:127-9

2038.   von Liebig JJ. 1847a. Über die bestandteile der flüssigkeiten des fleisches [About the ingredients of the fluids of the flesh]. Ann. Chem. Pharm. 62:281-369

2039.   von Liebig JJ. 1847b. On some new researches in animal chemistry. Extracted from a letter from Professor Liebig to Dr. A. W. Hofmann. Phil. Mag. Series 3 30:412-4

2040.   von Liebig JJ. 1853. Über kynurensäure [About kynurenic acid]. Ann. Chem. Pharm. 86:125-6

2041.   von Liebig JJ. 1870a. Ueber die gaehrung und die quelle der muskelkraft [About the fermentation and the source of muscle strength]. Ann. Chem. Pharm. 153:1-47

2042.   von Liebig JJ. 1870b. Sur la fermentation et la source de la force musculaire [The fermentation and the source of muscle strength]. Ann. de Chim. et de Phys. 23:5-49

2043.   von Ludwig WF. 1836. Über eine in neuerer zeit wiederholt hier vorgekommene form von halsentzündung [About one in recent times here repeatedly put arrived the form of sore throat]. Med. Corresp. Württ. 6:21-5

2044.   von Mayer JR, Joule JP, Carnot S. 1929. The discovery of the law of conservation of energy. Isis 13:18-44

2045.   von Middendorff AT. 1855. Die Isepiptesen Russlands. Grundlagen zur Erforschung der Zugzeiten und Zugrichtungen der Vögel Russlands [The Isepiptes of Russia. Basics for Researching the Migration Times and Directions of the Birds in Russia]. St. Petersburg: Buchdruckerei der K. Akademie der Wissenschaften. 143 pp.

2046.   von Mikulicz-Radecki J. 1897. Das operieren in sterilisierten zwirnhandschuhen und mit mundbinde; ein beitrag sur sicherung des aseptischenVerlaufsvon operationswunden [Operating in sterilized linen gloves and with a mouth covering; a contribution to securing an aseptic course in operative incisions]. Zentralbl. Chir. 24:713

2047.   von Mohl H. 1824. De Palmarum Structure [The Structure of Palms ]. In Historia Naturalis Palmarum, ed. KFPv Martius:I-LII. Leipzig: I.T.O. Weigel. Number of I-LII pp.

2048.   von Mohl H. 1833. Einige bemerkungen über die entwicklung und den bau cryptogamischer gewächse [Some remarks on the development and the construction of cryptogamic plants]. Flora (Jena) 16:33-46, 9-63, 5-78

2049.   von Mohl H. 1835. Ueber die Verbindung der Pflanzenzellen unter Einander [About the Connection of Plant Cells With Each Other]. Tübingen. 24 pp.

2050.   von Mohl H. 1837. Untersuchungen über die Anatomischen Verhältnisse des Chlorophylls [Studies on the Anatomical Relationships of Chlorophyll] Tübingen

2051.   von Mohl H. 1846. Über die saftbewegung im inneren der zellen [About the movement of sap in the interior of cells]. Bot. Zeitung (Berlin) 4:73-8, 89-94

2052.   von Mohl H. 1849. On the structure of the palm stem, Ray Society Reports and Papers on Botany, London

2053.   von Mohl H. 1855. Ueber den bau des chlorophylls [About the construction of chlorophyll]. Bot. Zeitung (Berlin) 13:89-99

2054.   von Recklinghausen A. 1863. Über eiter- und bindegewebskörperchen [About pus and connective tissue corpuscles]. Arch. Path. Anat. Physiol. Klin. Med. 28:157-97

2055.   von Recklinghausen F, Virchow R. 1882. Ueber die Multiplen Fibrome der Haut und Ihre Beziehung zu den Multiplen Neuromen. Festschrift zur Feier des Fünfundzwanzigjährigen Bestehens des Pathologischen Instituts zu Berlin. Herrn Rudolf Virchow [Concerning Multiple Fibromas in the Skin and Their Relationship to Multiple Neuromas. Commenorating the Twenty-fifth Anniversary of the Rudolf Virchow Institute of Pathology in Berlin]. In Virchow's Festschrift:138. Berlin: A. Hirschwald. Number of 138 pp.

2056.   von Rokitansky C. 1842-1846. Handbuch der Pathologischen Anatomie [Handbook of Pathological Anatomy]. Wien: Braumüller & Seidel

2057.   von Rokitansky C. 1852. Über einige der wichtigsten erkrankungen der arterien [About some of the most important diseases of the arteries]. Denkschr. Kais. Akad. Wiss. Math.-Naturwiss. Klass. 4:1-72

2058.   von Rokitansky C. 1854. Manual of Pathological Anatomy. London: The New Sydenham Society

2059.   von Schlechtendal DFL, Schiede CJW, Deppe F, von Chamisso LA. 1830-1834. De Plantis Mexicanis a G. Schiede [Plants of Mexico from G. Schiede]. Berlin

2060.   von Siebold ECJ. 1839. Versuch einer Geschichte der Geburtshülfe [Attempt at a History of Midwifery]. Berlin: Verlag von Theod. Chr. Friedr. Enslin. 368 pp.

2061.   von Siebold KTE. 1854a. Anatomy of the Invertebrates. Boston: Gould and Lincoln. 470 pp.

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